Reviewed, UniProtKB/Swiss-Prot Q8VNW3 (HPPA_CHLAA)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Synonyms:	vppA
Ordered Locus Names:	Caur_1306

Organism

Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) [Complete proteome] [HAMAP]

Taxonomic identifier

324602 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 775	775	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217014
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	65 – 85	21	Potential
	Transmembrane	108 – 128	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	185 – 205	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	288 – 308	21	Potential
	Transmembrane	327 – 347	21	Potential
	Transmembrane	359 – 379	21	Potential
	Transmembrane	413 – 433	21	Potential
	Transmembrane	442 – 462	21	Potential
	Transmembrane	508 – 528	21	Potential
	Transmembrane	555 – 575	21	Potential
	Transmembrane	622 – 642	21	Potential
	Transmembrane	644 – 664	21	Potential
	Transmembrane	710 – 730	21	Potential
	Transmembrane	735 – 755	21	Potential
Sites
	Site	502	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VNW3-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 509F7B985C75D931	FASTA	775	80,486
	10 20 30 40 50 60 MQDLNAVQSL AVWAVLVISL LGIGYAFFIR SQILAQDTGT PKMREVWGFI KTGANAYLSQ 70 80 90 100 110 120 QFRTISILIV ILTFVLAASV FIIPPTTEAV ERFGSKEAAT IWVAIGRAVA FLMGSLFSYA 130 140 150 160 170 180 VGFVGMNVAV EGNVRVAAAA RKGYNPALQV AYKSGSVTGM LTVGLGLLGG TLIFMVFGIA 190 200 210 220 230 240 APDALLGFGF GGSLIALFMR VGGGIYTKAA DVGADLVGKV EAGIPEDDPR NAAVIADLVG 250 260 270 280 290 300 DNVGDCAGMA ADVFESFEVT LVSALILGLV LGDAVVGTIG DGAYDLRFII FPLVLRAIGV 310 320 330 340 350 360 VASVIGNLFV TTDERKRNAM AAMNRGFYIA AGLAIAASAA ATPVFMVNEA TGEVDWRPFF 370 380 390 400 410 420 ATLSGVVLAI VLDKLTEYFT STHFSPVKET SKASQTGSAT NILSGLALGM ESSVWAILVI 430 440 450 460 470 480 SASIFTSVLI YAGEPAATQF TAILYGVSLT GIGMLLLTGN TISMDSFGPI SDNANGIGEM 490 500 510 520 530 540 AGLDKNARNV MDDLDAVGNT TKAVTKGIAI GSAVIAAVAL YGSYFTDVNK VLQQMINEGR 550 560 570 580 590 600 QGIELLASIN VAAPPVFIGL LIGGAVPFLF SALTIRAVSR AAAQIVNEVR RQFRIPGLME 610 620 630 640 650 660 GKVQPDYARA VQISTTAAQK ELISLGLIAV MVPIIVGFTL GVEALGGFLA GIILTGQLMA 670 680 690 700 710 720 VFQANAGGAW DNAKKYIEEG NFGGKHSEPH KAAVVGDTVG DPLKDTAGPA LNPMIKVINL 730 740 750 760 770 VALIIAPIVV TIEPGSPGVI IAMIICGAAL VWAIWQSKRE SEALKEIAQA PASAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of Chloroflexus aurantiacus J-10-fl." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Pierson B.K., Blankenship R.E., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Evidence for a wide distribution of proton-translocating pyrophosphatases among photosynthetic organisms." Perez-Castineira J.R., Losada M., Serrano A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 474-673.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000909 Genomic DNA. Translation: ABY34534.1. AJ251370 Genomic DNA. Translation: CAC80905.1.
RefSeq	YP_001634923.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5828410.
GenomeReviews	Gene locus Caur_1306 in contig CP000909_GR.
KEGG	cau:Caur_1306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PYLFGGI.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_CHLAA

Accession

Primary (citable) accession number: Q8VNW3
Secondary accession number(s): A9W9P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	March 18, 2008
Last modified:	November 3, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)