Q8VNU8 (HPPA_CHRVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1

Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase

Gene names

Name:	hppA
Synonyms:	vppA

Organism

Chromatium vinosum (Allochromatium vinosum)

Taxonomic identifier

1049 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

Protein attributes

Sequence length	197 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. HAMAP MF_01129
Subunit structure	Homodimer By similarity. HAMAP MF_01129
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›197	›197	K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217016
Regions
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Transmembrane	139 – 159	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
Sites
	Site	29	1	Determinant of potassium independence By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	197	1

Sequences

	Sequence		Length	Mass (Da)
	Q8VNU8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A22803C29E83EA58	FASTA	197	20,155
	10 20 30 40 50 60 AGGIAEMSGL PEDVRKTTDA LDAVGNTTKA VTKGYAIGSA GLAALVLFAD YTHNLEVAGK 70 80 90 100 110 120 MQVFSLSDPA VIIGLFIGGL VPYLFGAMAM EAVGRAAGSV VIEVRRQFRE IKGIMEGTAK 130 140 150 160 170 180 PDYSRAVDLL TKAAIKEMVV PSLLPILVPV AVAIGMNILM GDGAGIRALG GMLIGTIVTG 190 LFVAISMCTG GGAWDNA

References

[1]	"Evidence for a wide distribution of proton-translocating pyrophosphatases among photosynthetic organisms." Perez-Castineira J.R., Losada M., Serrano A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ251726 Genomic DNA. Translation: CAC80903.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01129. PPase-energized_pump. [Tree]
InterPro	IPR004131. PPase-energised_H-pump. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPPA_CHRVI

Accession

Primary (citable) accession number: Q8VNU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	March 1, 2002
Last modified:	May 31, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program