Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase

Gene

lacZ

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei108SubstrateUniRule annotation1
Metal bindingi207SodiumUniRule annotation1
Binding sitei207SubstrateUniRule annotation1
Sitei363Transition state stabilizerUniRule annotation1
Sitei397Transition state stabilizerUniRule annotation1
Metal bindingi422Magnesium 1UniRule annotation1
Metal bindingi424Magnesium 1UniRule annotation1
Active sitei467Proton donorUniRule annotation1
Metal bindingi467Magnesium 1UniRule annotation1
Binding sitei467SubstrateUniRule annotation1
Active sitei543NucleophileUniRule annotation1
Metal bindingi603Magnesium 2UniRule annotation1
Metal bindingi607Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi610SodiumUniRule annotation1
Binding sitei610SubstrateUniRule annotation1
Binding sitei1005SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Encoded oniPlasmid pMH110 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1293264.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669881 – 1029Beta-galactosidaseAdd BLAST1029

Proteomic databases

PRIDEiQ8VNN2.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi469008.ECD_00298.

Chemistry databases

BindingDBiQ8VNN2.

Structurei

3D structure databases

ProteinModelPortaliQ8VNN2.
SMRiQ8VNN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni543 – 546Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VNN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMITPSFPG NSLAVVLQRR DWENPGVTQL NRLAAHPPFA SWRNSEEART
60 70 80 90 100
DRPSQQLRSL NGEWRFAWFP APEAVPESWL ECDLPEADTV VVPSNWQMHG
110 120 130 140 150
YDAPIYTNVT YPITVNPPFV PTENPTGCYS LTFNVDESWL QEGQTRIIFD
160 170 180 190 200
GVNSAFHLWC NGRWVGYGQD SRLPSEFDLS AFLRAGENRL AVMVLRWSDG
210 220 230 240 250
SYLEDQDMWR MSGIFRDVSL LHKPTTQISD FHVATRFNDD FSRAVLEAEV
260 270 280 290 300
QMCGELRDYL RVTVSLWQGE TQVASGTAPF GGEIIDERGG YADRVTLRLN
310 320 330 340 350
VENPKLWSAE IPNLYRAVVE LHTADGTLIE AEACDVGFRE VRIENGLLLL
360 370 380 390 400
NGKPLLIRGV NRHEHHPLHG QVMDEQTMVQ DILLMKQNNF NAVRCSHYPN
410 420 430 440 450
HPLWYTLCDR YGLYVVDEAN IETHGMVPMN RLTDDPRWLP AMSERVTRMV
460 470 480 490 500
QRDRNHPSVI IWSLGNESGH GANHDALYRW IKSVDPSRPV QYEGGGADTT
510 520 530 540 550
ATDIICPMYA RVDEDQPFPA VPKWSIKKWL SLPGETRPLI LCEYAHAMGN
560 570 580 590 600
SLGGFAKYWQ AFRQYPRLQG GFVWDWVDQS LIKYDENGNP WSAYGGDFGD
610 620 630 640 650
TPNDRQFCMN GLVFADRTPH PALTEAKHQQ QFFQFRLSGQ TIEVTSEYLF
660 670 680 690 700
RHSDNELLHW MVALDGKPLA SGEVPLDVAP QGKQLIELPE LPQPESAGQL
710 720 730 740 750
WLTVRVVQPN ATAWSEAGHI SAWQQWRLAE NLSVTLPAAS HAIPHLTTSE
760 770 780 790 800
MDFCIELGNK RWQFNRQSGF LSQMWIGDKK QLLTPLRDQF TRAPLDNDIG
810 820 830 840 850
VSEATRIDPN AWVERWKAAG HYQAEAALLQ CTADTLADAV LITTAHAWQH
860 870 880 890 900
QGKTLFISRK TYRIDGSGQM AITVDVEVAS DTPHPARIGL NCQLAQVAER
910 920 930 940 950
VNWLGLGPQE NYPDRLTAAC FDRWDLPLSD MYTPYVFPSE NGLRCGTREL
960 970 980 990 1000
NYGPHQWRGD FQFNISRYSQ QQLMETSHRH LLHAEEGTWL NIDGFHMGIG
1010 1020
GDDSWSPSVS AEFQLSAGRY HYQLVWCQK
Length:1,029
Mass (Da):116,967
Last modified:March 1, 2002 - v1
Checksum:i46C5DF38322A2F26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ308295 Genomic DNA. Translation: CAC87491.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ308295 Genomic DNA. Translation: CAC87491.1.

3D structure databases

ProteinModelPortaliQ8VNN2.
SMRiQ8VNN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi469008.ECD_00298.

Chemistry databases

BindingDBiQ8VNN2.
ChEMBLiCHEMBL1293264.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEiQ8VNN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_ECOLX
AccessioniPrimary (citable) accession number: Q8VNN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.