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Q8VNN2

- BGAL_ECOLX

UniProt

Q8VNN2 - BGAL_ECOLX

Protein

Beta-galactosidase

Gene

lacZ

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei108 – 1081SubstrateUniRule annotation
    Metal bindingi207 – 2071SodiumUniRule annotation
    Binding sitei207 – 2071SubstrateUniRule annotation
    Sitei363 – 3631Transition state stabilizerUniRule annotation
    Sitei397 – 3971Transition state stabilizerUniRule annotation
    Metal bindingi422 – 4221Magnesium 1UniRule annotation
    Metal bindingi424 – 4241Magnesium 1UniRule annotation
    Active sitei467 – 4671Proton donorUniRule annotation
    Metal bindingi467 – 4671Magnesium 1UniRule annotation
    Binding sitei467 – 4671SubstrateUniRule annotation
    Active sitei543 – 5431NucleophileUniRule annotation
    Metal bindingi603 – 6031Magnesium 2UniRule annotation
    Metal bindingi607 – 6071Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi610 – 6101SodiumUniRule annotation
    Binding sitei610 – 6101SubstrateUniRule annotation
    Binding sitei1005 – 10051SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Encoded oniPlasmid pMH110 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10291029Beta-galactosidasePRO_0000366988Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VNN2.
    SMRiQ8VNN2. Positions 19-1029.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni543 – 5464Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VNN2-1 [UniParc]FASTAAdd to Basket

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    MTMITPSFPG NSLAVVLQRR DWENPGVTQL NRLAAHPPFA SWRNSEEART     50
    DRPSQQLRSL NGEWRFAWFP APEAVPESWL ECDLPEADTV VVPSNWQMHG 100
    YDAPIYTNVT YPITVNPPFV PTENPTGCYS LTFNVDESWL QEGQTRIIFD 150
    GVNSAFHLWC NGRWVGYGQD SRLPSEFDLS AFLRAGENRL AVMVLRWSDG 200
    SYLEDQDMWR MSGIFRDVSL LHKPTTQISD FHVATRFNDD FSRAVLEAEV 250
    QMCGELRDYL RVTVSLWQGE TQVASGTAPF GGEIIDERGG YADRVTLRLN 300
    VENPKLWSAE IPNLYRAVVE LHTADGTLIE AEACDVGFRE VRIENGLLLL 350
    NGKPLLIRGV NRHEHHPLHG QVMDEQTMVQ DILLMKQNNF NAVRCSHYPN 400
    HPLWYTLCDR YGLYVVDEAN IETHGMVPMN RLTDDPRWLP AMSERVTRMV 450
    QRDRNHPSVI IWSLGNESGH GANHDALYRW IKSVDPSRPV QYEGGGADTT 500
    ATDIICPMYA RVDEDQPFPA VPKWSIKKWL SLPGETRPLI LCEYAHAMGN 550
    SLGGFAKYWQ AFRQYPRLQG GFVWDWVDQS LIKYDENGNP WSAYGGDFGD 600
    TPNDRQFCMN GLVFADRTPH PALTEAKHQQ QFFQFRLSGQ TIEVTSEYLF 650
    RHSDNELLHW MVALDGKPLA SGEVPLDVAP QGKQLIELPE LPQPESAGQL 700
    WLTVRVVQPN ATAWSEAGHI SAWQQWRLAE NLSVTLPAAS HAIPHLTTSE 750
    MDFCIELGNK RWQFNRQSGF LSQMWIGDKK QLLTPLRDQF TRAPLDNDIG 800
    VSEATRIDPN AWVERWKAAG HYQAEAALLQ CTADTLADAV LITTAHAWQH 850
    QGKTLFISRK TYRIDGSGQM AITVDVEVAS DTPHPARIGL NCQLAQVAER 900
    VNWLGLGPQE NYPDRLTAAC FDRWDLPLSD MYTPYVFPSE NGLRCGTREL 950
    NYGPHQWRGD FQFNISRYSQ QQLMETSHRH LLHAEEGTWL NIDGFHMGIG 1000
    GDDSWSPSVS AEFQLSAGRY HYQLVWCQK 1029
    Length:1,029
    Mass (Da):116,967
    Last modified:March 1, 2002 - v1
    Checksum:i46C5DF38322A2F26
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ308295 Genomic DNA. Translation: CAC87491.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ308295 Genomic DNA. Translation: CAC87491.1 .

    3D structure databases

    ProteinModelPortali Q8VNN2.
    SMRi Q8VNN2. Positions 19-1029.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1293264.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG3250.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "pMH11, A tool for gene disruption and expression analysis in Azorhizobium caulinodans."
      D'Haeze W., Verplancke C., Mironov V., Holsters M.
      Plasmid 47:88-93(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiBGAL_ECOLX
    AccessioniPrimary (citable) accession number: Q8VNN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3