Reviewed,
UniProtKB/Swiss-Prot Q8VNN2 (BGAL_ECOLX)
Last modified
June 16, 2009.
Version 39.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
 Customize display | text xml rdf/xml gff fasta |
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Beta-galactosidase Short name=Beta-gal EC=3.2.1.23 Alternative name(s): Lactase | ||
| Gene names |
| ||
| Encoded on | Plasmid pMH11 | ||
| Organism | Escherichia coli | ||
| Taxonomic identifier | 562 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 1029 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687 |
| Cofactor | Binds 2 magnesium ions per monomer By similarity. Binds 1 sodium ion per monomer By similarity. |
| Subunit structure | Homotetramer By similarity. |
| Sequence similarities | Belongs to the glycosyl hydrolase 2 family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Magnesium Metal-binding Sodium |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Plasmid |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | beta-galactosidase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate bindingInferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: HAMAP sodium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1029 | 1029 | Beta-galactosidase HAMAP MF_01687 | PRO_0000366988 | |||||
Regions | |||||||||
| Region | 543 – 546 | 4 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 467 | 1 | Proton donor By similarity | ||||||
| Active site | 543 | 1 | Nucleophile By similarity | ||||||
| Metal binding | 207 | 1 | Sodium By similarity | ||||||
| Metal binding | 422 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 424 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 467 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 603 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 607 | 1 | Sodium; via carbonyl oxygen By similarity | ||||||
| Metal binding | 610 | 1 | Sodium By similarity | ||||||
| Binding site | 108 | 1 | Substrate By similarity | ||||||
| Binding site | 207 | 1 | Substrate By similarity | ||||||
| Binding site | 467 | 1 | Substrate By similarity | ||||||
| Binding site | 610 | 1 | Substrate By similarity | ||||||
| Binding site | 1005 | 1 | Substrate By similarity | ||||||
| Site | 363 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 397 | 1 | Transition state stabilizer By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "pMH11, A tool for gene disruption and expression analysis in Azorhizobium caulinodans." D'Haeze W., Verplancke C., Mironov V., Holsters M. Plasmid 47:88-93(2002) [PubMed: 11982330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AJ308295 Genomic DNA. Translation: CAC87491.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BGL based on UniProtKB P00722. |
| SMR | Q8VNN2. Positions 19-1029. |
| ModBase | Search... |
Family and domain databases | |
| HAMAP | MF_01687. [Tree] |
| InterPro | IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| Pfam | PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view] |
| PRINTS | PR00132. GLHYDRLASE2. |
| PROSITE | PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | BGAL_ECOLX | ||||||||
| Accession | Primary (citable) accession number: Q8VNN2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
