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Q8VNN2

- BGAL_ECOLX

UniProt

Q8VNN2 - BGAL_ECOLX

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Protein
Beta-galactosidase
Gene
lacZ
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei108 – 1081Substrate By similarity
Metal bindingi207 – 2071Sodium By similarity
Binding sitei207 – 2071Substrate By similarity
Sitei363 – 3631Transition state stabilizer By similarity
Sitei397 – 3971Transition state stabilizer By similarity
Metal bindingi422 – 4221Magnesium 1 By similarity
Metal bindingi424 – 4241Magnesium 1 By similarity
Active sitei467 – 4671Proton donor By similarity
Metal bindingi467 – 4671Magnesium 1 By similarity
Binding sitei467 – 4671Substrate By similarity
Active sitei543 – 5431Nucleophile By similarity
Metal bindingi603 – 6031Magnesium 2 By similarity
Metal bindingi607 – 6071Sodium; via carbonyl oxygen By similarity
Metal bindingi610 – 6101Sodium By similarity
Binding sitei610 – 6101Substrate By similarity
Binding sitei1005 – 10051Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Encoded oniPlasmid pMH110 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Beta-galactosidaseUniRule annotation
PRO_0000366988Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8VNN2.
SMRiQ8VNN2. Positions 19-1029.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni543 – 5464Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VNN2-1 [UniParc]FASTAAdd to Basket

« Hide

MTMITPSFPG NSLAVVLQRR DWENPGVTQL NRLAAHPPFA SWRNSEEART     50
DRPSQQLRSL NGEWRFAWFP APEAVPESWL ECDLPEADTV VVPSNWQMHG 100
YDAPIYTNVT YPITVNPPFV PTENPTGCYS LTFNVDESWL QEGQTRIIFD 150
GVNSAFHLWC NGRWVGYGQD SRLPSEFDLS AFLRAGENRL AVMVLRWSDG 200
SYLEDQDMWR MSGIFRDVSL LHKPTTQISD FHVATRFNDD FSRAVLEAEV 250
QMCGELRDYL RVTVSLWQGE TQVASGTAPF GGEIIDERGG YADRVTLRLN 300
VENPKLWSAE IPNLYRAVVE LHTADGTLIE AEACDVGFRE VRIENGLLLL 350
NGKPLLIRGV NRHEHHPLHG QVMDEQTMVQ DILLMKQNNF NAVRCSHYPN 400
HPLWYTLCDR YGLYVVDEAN IETHGMVPMN RLTDDPRWLP AMSERVTRMV 450
QRDRNHPSVI IWSLGNESGH GANHDALYRW IKSVDPSRPV QYEGGGADTT 500
ATDIICPMYA RVDEDQPFPA VPKWSIKKWL SLPGETRPLI LCEYAHAMGN 550
SLGGFAKYWQ AFRQYPRLQG GFVWDWVDQS LIKYDENGNP WSAYGGDFGD 600
TPNDRQFCMN GLVFADRTPH PALTEAKHQQ QFFQFRLSGQ TIEVTSEYLF 650
RHSDNELLHW MVALDGKPLA SGEVPLDVAP QGKQLIELPE LPQPESAGQL 700
WLTVRVVQPN ATAWSEAGHI SAWQQWRLAE NLSVTLPAAS HAIPHLTTSE 750
MDFCIELGNK RWQFNRQSGF LSQMWIGDKK QLLTPLRDQF TRAPLDNDIG 800
VSEATRIDPN AWVERWKAAG HYQAEAALLQ CTADTLADAV LITTAHAWQH 850
QGKTLFISRK TYRIDGSGQM AITVDVEVAS DTPHPARIGL NCQLAQVAER 900
VNWLGLGPQE NYPDRLTAAC FDRWDLPLSD MYTPYVFPSE NGLRCGTREL 950
NYGPHQWRGD FQFNISRYSQ QQLMETSHRH LLHAEEGTWL NIDGFHMGIG 1000
GDDSWSPSVS AEFQLSAGRY HYQLVWCQK 1029
Length:1,029
Mass (Da):116,967
Last modified:March 1, 2002 - v1
Checksum:i46C5DF38322A2F26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ308295 Genomic DNA. Translation: CAC87491.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ308295 Genomic DNA. Translation: CAC87491.1 .

3D structure databases

ProteinModelPortali Q8VNN2.
SMRi Q8VNN2. Positions 19-1029.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1293264.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3250.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "pMH11, A tool for gene disruption and expression analysis in Azorhizobium caulinodans."
    D'Haeze W., Verplancke C., Mironov V., Holsters M.
    Plasmid 47:88-93(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBGAL_ECOLX
AccessioniPrimary (citable) accession number: Q8VNN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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