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Q8VNN2

- BGAL_ECOLX

UniProt

Q8VNN2 - BGAL_ECOLX

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei108 – 1081SubstrateUniRule annotation
Metal bindingi207 – 2071SodiumUniRule annotation
Binding sitei207 – 2071SubstrateUniRule annotation
Sitei363 – 3631Transition state stabilizerUniRule annotation
Sitei397 – 3971Transition state stabilizerUniRule annotation
Metal bindingi422 – 4221Magnesium 1UniRule annotation
Metal bindingi424 – 4241Magnesium 1UniRule annotation
Active sitei467 – 4671Proton donorUniRule annotation
Metal bindingi467 – 4671Magnesium 1UniRule annotation
Binding sitei467 – 4671SubstrateUniRule annotation
Active sitei543 – 5431NucleophileUniRule annotation
Metal bindingi603 – 6031Magnesium 2UniRule annotation
Metal bindingi607 – 6071Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi610 – 6101SodiumUniRule annotation
Binding sitei610 – 6101SubstrateUniRule annotation
Binding sitei1005 – 10051SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Encoded oniPlasmid pMH110 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Beta-galactosidasePRO_0000366988Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8VNN2.
SMRiQ8VNN2. Positions 19-1029.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni543 – 5464Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VNN2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTMITPSFPG NSLAVVLQRR DWENPGVTQL NRLAAHPPFA SWRNSEEART
60 70 80 90 100
DRPSQQLRSL NGEWRFAWFP APEAVPESWL ECDLPEADTV VVPSNWQMHG
110 120 130 140 150
YDAPIYTNVT YPITVNPPFV PTENPTGCYS LTFNVDESWL QEGQTRIIFD
160 170 180 190 200
GVNSAFHLWC NGRWVGYGQD SRLPSEFDLS AFLRAGENRL AVMVLRWSDG
210 220 230 240 250
SYLEDQDMWR MSGIFRDVSL LHKPTTQISD FHVATRFNDD FSRAVLEAEV
260 270 280 290 300
QMCGELRDYL RVTVSLWQGE TQVASGTAPF GGEIIDERGG YADRVTLRLN
310 320 330 340 350
VENPKLWSAE IPNLYRAVVE LHTADGTLIE AEACDVGFRE VRIENGLLLL
360 370 380 390 400
NGKPLLIRGV NRHEHHPLHG QVMDEQTMVQ DILLMKQNNF NAVRCSHYPN
410 420 430 440 450
HPLWYTLCDR YGLYVVDEAN IETHGMVPMN RLTDDPRWLP AMSERVTRMV
460 470 480 490 500
QRDRNHPSVI IWSLGNESGH GANHDALYRW IKSVDPSRPV QYEGGGADTT
510 520 530 540 550
ATDIICPMYA RVDEDQPFPA VPKWSIKKWL SLPGETRPLI LCEYAHAMGN
560 570 580 590 600
SLGGFAKYWQ AFRQYPRLQG GFVWDWVDQS LIKYDENGNP WSAYGGDFGD
610 620 630 640 650
TPNDRQFCMN GLVFADRTPH PALTEAKHQQ QFFQFRLSGQ TIEVTSEYLF
660 670 680 690 700
RHSDNELLHW MVALDGKPLA SGEVPLDVAP QGKQLIELPE LPQPESAGQL
710 720 730 740 750
WLTVRVVQPN ATAWSEAGHI SAWQQWRLAE NLSVTLPAAS HAIPHLTTSE
760 770 780 790 800
MDFCIELGNK RWQFNRQSGF LSQMWIGDKK QLLTPLRDQF TRAPLDNDIG
810 820 830 840 850
VSEATRIDPN AWVERWKAAG HYQAEAALLQ CTADTLADAV LITTAHAWQH
860 870 880 890 900
QGKTLFISRK TYRIDGSGQM AITVDVEVAS DTPHPARIGL NCQLAQVAER
910 920 930 940 950
VNWLGLGPQE NYPDRLTAAC FDRWDLPLSD MYTPYVFPSE NGLRCGTREL
960 970 980 990 1000
NYGPHQWRGD FQFNISRYSQ QQLMETSHRH LLHAEEGTWL NIDGFHMGIG
1010 1020
GDDSWSPSVS AEFQLSAGRY HYQLVWCQK
Length:1,029
Mass (Da):116,967
Last modified:March 1, 2002 - v1
Checksum:i46C5DF38322A2F26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ308295 Genomic DNA. Translation: CAC87491.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ308295 Genomic DNA. Translation: CAC87491.1 .

3D structure databases

ProteinModelPortali Q8VNN2.
SMRi Q8VNN2. Positions 19-1029.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1293264.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3250.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "pMH11, A tool for gene disruption and expression analysis in Azorhizobium caulinodans."
    D'Haeze W., Verplancke C., Mironov V., Holsters M.
    Plasmid 47:88-93(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiBGAL_ECOLX
AccessioniPrimary (citable) accession number: Q8VNN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: October 1, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3