Q8VNJ8 (HPPA_HELCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative K(+)-stimulated pyrophosphate-energized sodium pump
EC=3.6.1.1

Alternative name(s):
Membrane-bound sodium-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=Na(+)-PPase

Gene names

Name:	hppA
Synonyms:	vppA

Organism

Heliobacterium chlorum

Taxonomic identifier

2698 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Heliobacteriaceae › Heliobacterium

Protein attributes

Sequence length	197 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane By similarity. HAMAP MF_01129
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. HAMAP MF_01129
Enzyme regulation	Requires K+ for maximal activity By similarity. HAMAP MF_01129
Subunit structure	Homodimer By similarity. HAMAP MF_01129
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Potassium Sodium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›197	›197	Putative K(+)-stimulated pyrophosphate-energized sodium pump HAMAP MF_01129		PRO_0000217004
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
Sites
	Site	29	1	Determinant of potassium dependence By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	197	1

Sequences

	Sequence		Length	Mass (Da)
	Q8VNJ8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DA4FC7F465481D6E	FASTA	197	20,230
	10 20 30 40 50 60 AGGIAEMAEL GPEVRKKTDK LDAVGNTTAA VAKGFAIGSA ALTALALFTA FAEEVVKSPG 70 80 90 100 110 120 VSSALHGSHF VLNLLEPKII IGLFIGGTVP FLFCAFAMEA VGKAAFEMIG EVRRQFREIP 130 140 150 160 170 180 GLMEGKAKPD YARCVDISTR AAIRQMIAPG LLAVITPLIV GFGFGAQALG GMLAGVTVAG 190 VLLAIFMANA GGAWDHA

References

[1]	"Evidence for a wide distribution of proton-translocating pyrophosphatases among photosynthetic organisms." Perez-Castineira J.R., Losada M., Serrano A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3682.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ251371 Genomic DNA. Translation: CAC80906.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01129. PPase-energized_pump. [Tree]
InterPro	IPR004131. PPase-energised_H-pump. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPPA_HELCL

Accession

Primary (citable) accession number: Q8VNJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	March 1, 2002
Last modified:	May 31, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program