Reviewed, UniProtKB/Swiss-Prot Q8VL08 (CHEB2_AZOBR)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon
    EC=3.1.1.61

Gene names

Name:

cheB

Organism

Azospirillum brasilense

Taxonomic identifier

192 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon HAMAP MF_00099		PRO_0000157974
Regions
	Domain	27 – 145	119	Response regulatory
	Domain	191 – 393	203	CheB-type methylesterase
Sites
	Active site	211	1	By similarity
	Active site	238	1	By similarity
	Active site	335	1	By similarity
Amino acid modifications
	Modified residue	78	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VL08-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 55FBCF8D432A02BD	FASTA	394	40,986
	10 20 30 40 50 60 MSDGFGRPPP PAPAGHPTGA AGGDPVRVMV VDDSAVIRGL LTRALEGDTE IRVVASVGDG 70 80 90 100 110 120 QMAVNALQRN SLDVIVLDIE MPVMDGLTAI PKLLAVAPQV KIIMASTLTL RGADISMRCL 130 140 150 160 170 180 SAGAADYIPK PTSTREIGGA DAFKRELVSK VKALGAAARR AGSRTRGELR PLNPVPAAFL 190 200 210 220 230 240 KREPPPVTLR PAPAVGSVGQ VKPDVIAIGS STGGPQALFE VLAHLKTGVT QPILITQHMP 250 260 270 280 290 300 ATFTTILAEH ITRQCGMNAQ EAKDGEPVVP GRCYIAPGDF HMLVTQRAGA NVISLTKDPP 310 320 330 340 350 360 ENFCRPAVDP MMRSILRAFG GRKVLACILT GMGQDGLKGC TEVVNAGGTL IAQDEASSVV 370 380 390 WGMPGAVAQA GICSAVLPLK EIGPYIRKFA SRAA

References

[1]

"A major chemotaxis gene cluster in Azospirillum brasilense and relationships between chemotaxis operons in alpha-proteobacteria." Hauwaerts D., Alexandre G., Das S.K., Vanderleyden J., Zhulin I.B. FEMS Microbiol. Lett. 208:61-67(2002) [PubMed: 11934495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29145 / Sp7 / DSM 1690 / IMET 11303.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF450326 Genomic DNA. Translation: AAL47024.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHD based on UniProtKB P04042.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.61. 1315.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_AZOBR

Accession

Primary (citable) accession number: Q8VL08

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)