Q8VIM5 (MYCD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myocardin

Alternative name(s):
Basic SAP coiled-coil transcription activator 2
SRF cofactor protein

Gene names

Name:	Myocd
Synonyms:	Bsac2, Mycd, Srfcp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	935 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). Isoform 1 mediates the cardiac transcription factor MEF2C-dependent transcription. Isoform 1 and isoform 3 are more active than isoform 2 and isoform 4 in stimulating cardiac muscle promoters. Ref.1 Ref.5 Ref.8 Ref.11 Ref.13
Subunit structure	Homodimer. Interacts with MLLT7/FOXO4 By similarity. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation. Interacts (via C-terminal) with EP300 (via CREB-binding domain). Interacts with HDAC4 and HDAC5. Interacts with MEF2C. Ref.1 Ref.8 Ref.10 Ref.11
Subcellular location	Nucleus speckle. Note: Nuclear, with a punctate intranuclear pattern with exclusion from nuclei. Ref.1 Ref.10
Tissue specificity	Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder and uterus. Isoform 1 and isoform 3 are predominantly expressed in cardiac muscle whereas isoform 4 and isoform 5 are predominantly expressed in SMC-rich tissues. Isoform 3 is the most abundant isoform in the heart from embryo to adult. Ref.1 Ref.3 Ref.5 Ref.8 Ref.13
Developmental stage	Detected in the cardiac crescent at 7.75 dpc and in the linear heart tube at 8.0 dpc and the developing atrial and aortic ventricular chambers until birth. Also detected in a subset of vascular and visceral smooth muscle cells: aortic arch arteries at 9.5 dpc; walls of the esophagus, dorsal aorta, pulmonary outflow tract, lung, gut, stomach, small intestine, bladder, and the head mesenchyme at 13.5 dpc until birth. Not detected in skeletal muscle cells. Ref.1 Ref.5
Domain	The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
Post-translational modification	Phosphorylation regulates negatively transcriptional activity.
Sequence similarities	Contains 3 RPEL repeats. Contains 1 SAP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac ventricle development Inferred from mutant phenotype PubMed 21357509. Source: MGI cell growth involved in cardiac muscle cell development Inferred from electronic annotation. Source: Compara cellular component maintenance Inferred from mutant phenotype PubMed 19850880. Source: MGI cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to hypoxia Inferred from electronic annotation. Source: Compara digestive tract development Inferred from mutant phenotype PubMed 21357509. Source: MGI ductus arteriosus closure Inferred from mutant phenotype PubMed 18188448. Source: MGI hepatic stellate cell activation Inferred from electronic annotation. Source: Compara lung alveolus development Inferred from mutant phenotype PubMed 21357509. Source: MGI negative regulation of beta-amyloid clearance Inferred from electronic annotation. Source: Compara negative regulation of cardiac muscle cell apoptotic process Inferred from mutant phenotype PubMed 19850880. Source: MGI negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: Compara negative regulation of myotube differentiation Inferred from mutant phenotype PubMed 17940050. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction Ref.10PubMed 17940050. Source: MGI positive regulation of DNA binding Inferred from genetic interaction PubMed 19342595. Source: MGI positive regulation of cardiac muscle cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of cardiac vascular smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from direct assay Ref.1. Source: MGI positive regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.5PubMed 15798203Ref.11PubMed 17030628PubMed 17940050PubMed 18945672. Source: MGI positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transforming growth factor beta receptor signaling pathway Inferred from direct assay PubMed 16224064. Source: BHF-UCL regulation of cell growth by extracellular stimulus Inferred from direct assay Ref.1. Source: MGI regulation of histone acetylation Inferred from direct assay Ref.10. Source: UniProtKB regulation of myoblast differentiation Inferred from mutant phenotype Ref.5. Source: MGI smooth muscle cell differentiation Inferred from mutant phenotype PubMed 18188448PubMed 21357509. Source: MGI urinary bladder development Inferred from mutant phenotype PubMed 21357509. Source: MGI uterus development Inferred from mutant phenotype PubMed 21357509. Source: MGI vasculogenesis Inferred from mutant phenotype PubMed 12867591. Source: MGI ventricular cardiac muscle cell differentiation Inferred from mutant phenotype PubMed 21357509. Source: MGI
Cellular_component	SRF-myogenin-E12 complex Inferred by curator Ref.1. Source: BHF-UCL nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA polymerase II transcription factor binding transcription factor activity Inferred from direct assay Ref.1. Source: BHF-UCL core promoter sequence-specific DNA binding Inferred from electronic annotation. Source: Compara transcription coactivator activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VIM5-1)

Also known as: MYOCD-v2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VIM5-2)

Also known as: BSAC2A; Myocardin A; MYOCD-v5;

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     683-683: Q → QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPK

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8VIM5-3)

Also known as: =Myocardin A; MYOCD-v1;

The sequence of this isoform differs from the canonical sequence as follows:
     683-683: Q → QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPK

Isoform 4 (identifier: Q8VIM5-4)

Also known as: MYOCD-v3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     683-683: Q → QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPK

Isoform 5 (identifier: Q8VIM5-5)

Also known as: MYOCD-v4;

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 935	935	Myocardin		PRO_0000126632
Regions
	Repeat	18 – 43	26	RPEL 1
	Repeat	62 – 87	26	RPEL 2
	Repeat	106 – 131	26	RPEL 3
	Domain	380 – 414	35	SAP
	Region	153 – 201	49	HDAC5-binding
	Coiled coil	287 – 322	36	Potential
	Coiled coil	519 – 563	45	Potential
	Motif	12 – 27	16	MEF2C-binding
	Compositional bias	287 – 320	34	Gln-rich
	Compositional bias	300 – 320	21	Poly-Gln
Amino acid modifications
	Modified residue	454	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	458	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	462	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	466	1	Phosphoserine; by GSK3-beta
	Modified residue	624	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	628	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	632	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	636	1	Phosphoserine; by GSK3-beta Ref.9
	Modified residue	812	1	Phosphoserine; by MAPK1 AND MAPK3 Ref.12
	Modified residue	859	1	Phosphoserine; by MAPK1 and MAPK3 Ref.12
	Modified residue	866	1	Phosphoserine; by MAPK1 and MAPK3 Ref.12
	Modified residue	893	1	Phosphothreonine; by MAPK1 and MAPK3 Ref.12
Natural variations
	Alternative sequence	1 – 128	128	Missing in isoform 2.		VSP_007662
	Alternative sequence	1 – 79	79	Missing in isoform 4 and isoform 5.		VSP_041684
	Alternative sequence	683	1	Q → QNSGAHEGHSSSFSSPASSL HQPFSGTQADSSHSAGLNPC PKSPSIHPK in isoform 2, isoform 3 and isoform 4.		VSP_007663
Experimental info
	Mutagenesis	387 – 389	3	ELR → PSF: Activation of ANF promoter abolished, no effect on SM22 promoter. Ref.1
	Mutagenesis	408 – 410	3	DRL → PGH: Activation of ANF promoter abolished, no effect on SM22 promoter. Ref.1
	Mutagenesis	812	1	S → A: No effect on SRF activation. No effect on SRF activation, on interaction with EP300 and on SM-promoter activation; when associated with A-859; A-866 and A-893. Ref.12
	Mutagenesis	812	1	S → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-859; D-866 and D-893. Ref.12
	Mutagenesis	859	1	S → A: No effect on SRF activation; No effect on SRF activation. No effect on SRF activation, on interaction with EP300 and on SM-promoter activation; when associated with A-812, A-866 and A-893. Ref.12
	Mutagenesis	859	1	S → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-812; D-866 and D-893. Ref.12
	Mutagenesis	866	1	S → A: No effect on SRF activation; No effect on SRF activation, on interaction with EP300 and on SM-promoter activation; when associated with A-812; A-859 and A-893. Ref.12
	Mutagenesis	866	1	S → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-812; D-859 and D-893. Ref.12
	Mutagenesis	893	1	T → A: No effect on SRF activation; No effect on SRF activation, on interaction with EP300 and on SM-specific genes trancription; when associated with A-812; A-859 and A-866. Ref.12
	Mutagenesis	893	1	T → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-812; D-859 and D-866. Ref.12
	Sequence conflict	116	1	P → Q in AAK71683. Ref.1
	Sequence conflict	794	1	D → G in AAK71683. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (MYOCD-v2) [UniParc]. Last modified July 27, 2011. Version 2. Checksum: A8FCFC68A923A5CB	FASTA	935	101,400
	10 20 30 40 50 60 MTLLGSEHSL LIRRKFRSVL QLRLQQRRTQ EQLANQGLIP PLKGPTEFHD PRKQLDSAKT 70 80 90 100 110 120 EDSLRRKGRN RSDRASLVTM HILQASTAER SIPTAQMKLK RARLADDLNE KIALRPGPLE 130 140 150 160 170 180 LVEKNILPMD SSVKEAIKGT EVSLSKAADA FAFEDDSSRD GLSPDQARSE DPQGSTGSTP 190 200 210 220 230 240 DIKSTEAPLD TIQDLTPGSE SDKNDAASQP GNQSDPGKQV LGPLSTPIPV HTAVKSKSLG 250 260 270 280 290 300 DSKNRHKKPK DPKPKVKKLK YHQYIPPDQK AEKSPPPMDS AYARLLQQQQ LFLQLQILSQ 310 320 330 340 350 360 QQQQQQQQQQ QQQQQQQQQQ RFSYPGMHQT HLKEPNEQMA RNPNPSSTPL SNTPLSPVKN 370 380 390 400 410 420 SISGQTGVSS LKPGPLPPNL DDLKVSELRQ QLRIRGLPVS GTKTALVDRL RPFQDCAGNP 430 440 450 460 470 480 VPNFGDITTV TFPVTPNTLP SYQSSPTGFY HFGSTSSSPP ISPASSDLSA AGSLPDTFTD 490 500 510 520 530 540 ASPGFGLHAS PVPACTDESL LSSLNGGSGP SEPDGLDSEK DKMLVEKQKV INQLTWKLRQ 550 560 570 580 590 600 EQRQVEELRM QLQKQKSSCS DQKPLPFLAT TIKQEDVSSC PFAPQQASGK GQGHSSDSPP 610 620 630 640 650 660 PACETAQLLP HCVESSGQTH VLSSTFLSPQ CSPQHSPLGG LKSPQHISLP PSPNNHYFLA 670 680 690 700 710 720 SSSGAQRENH GVSSPSSSQG CAQMTGLQSS DKVGPTFSIP SPTFSKSSSA VSDITQPPSY 730 740 750 760 770 780 EDAVKQQMTR SQQMDELLDV LIESGEMPAD AREDHSCLQK IPKIPGSSCS PTAIPPKPSA 790 800 810 820 830 840 SFEQASSGGQ MAFDHYANDS DEHLEVLLNS HSPIGKVSDV TLLKIGSEEP PFDSIMDGFP 850 860 870 880 890 900 GKAAEDLFSA HELLPGPLSP MHAQLSPPSV DSSGLQLSFT ESPWETMEWL DLTPPSSTPG 910 920 930 FSNLTSSGPS IFNIDFLDVT DLNLNSPMDL HLQQW
	Isoform 2 (BSAC2A) (Myocardin A) (MYOCD-v5) [UniParc]. Checksum: 73C3AAB62E61DB33	FASTA	855	91,592
	10 20 30 40 50 60 MDSSVKEAIK GTEVSLSKAA DAFAFEDDSS RDGLSPDQAR SEDPQGSTGS TPDIKSTEAP 70 80 90 100 110 120 LDTIQDLTPG SESDKNDAAS QPGNQSDPGK QVLGPLSTPI PVHTAVKSKS LGDSKNRHKK 130 140 150 160 170 180 PKDPKPKVKK LKYHQYIPPD QKAEKSPPPM DSAYARLLQQ QQLFLQLQIL SQQQQQQQQQ 190 200 210 220 230 240 QQQQQQQQQQ QQRFSYPGMH QTHLKEPNEQ MARNPNPSST PLSNTPLSPV KNSISGQTGV 250 260 270 280 290 300 SSLKPGPLPP NLDDLKVSEL RQQLRIRGLP VSGTKTALVD RLRPFQDCAG NPVPNFGDIT 310 320 330 340 350 360 TVTFPVTPNT LPSYQSSPTG FYHFGSTSSS PPISPASSDL SAAGSLPDTF TDASPGFGLH 370 380 390 400 410 420 ASPVPACTDE SLLSSLNGGS GPSEPDGLDS EKDKMLVEKQ KVINQLTWKL RQEQRQVEEL 430 440 450 460 470 480 RMQLQKQKSS CSDQKPLPFL ATTIKQEDVS SCPFAPQQAS GKGQGHSSDS PPPACETAQL 490 500 510 520 530 540 LPHCVESSGQ THVLSSTFLS PQCSPQHSPL GGLKSPQHIS LPPSPNNHYF LASSSGAQRE 550 560 570 580 590 600 NHGVSSPSSS QGCAQNSGAH EGHSSSFSSP ASSLHQPFSG TQADSSHSAG LNPCPKSPSI 610 620 630 640 650 660 HPKMTGLQSS DKVGPTFSIP SPTFSKSSSA VSDITQPPSY EDAVKQQMTR SQQMDELLDV 670 680 690 700 710 720 LIESGEMPAD AREDHSCLQK IPKIPGSSCS PTAIPPKPSA SFEQASSGGQ MAFDHYANDS 730 740 750 760 770 780 DEHLEVLLNS HSPIGKVSDV TLLKIGSEEP PFDSIMDGFP GKAAEDLFSA HELLPGPLSP 790 800 810 820 830 840 MHAQLSPPSV DSSGLQLSFT ESPWETMEWL DLTPPSSTPG FSNLTSSGPS IFNIDFLDVT 850 DLNLNSPMDL HLQQW
	Isoform 3 (=Myocardin A) (MYOCD-v1) [UniParc]. Checksum: 7640D2863F9DB517	FASTA	983	106,223
	10 20 30 40 50 60 MTLLGSEHSL LIRRKFRSVL QLRLQQRRTQ EQLANQGLIP PLKGPTEFHD PRKQLDSAKT 70 80 90 100 110 120 EDSLRRKGRN RSDRASLVTM HILQASTAER SIPTAQMKLK RARLADDLNE KIALRPGPLE 130 140 150 160 170 180 LVEKNILPMD SSVKEAIKGT EVSLSKAADA FAFEDDSSRD GLSPDQARSE DPQGSTGSTP 190 200 210 220 230 240 DIKSTEAPLD TIQDLTPGSE SDKNDAASQP GNQSDPGKQV LGPLSTPIPV HTAVKSKSLG 250 260 270 280 290 300 DSKNRHKKPK DPKPKVKKLK YHQYIPPDQK AEKSPPPMDS AYARLLQQQQ LFLQLQILSQ 310 320 330 340 350 360 QQQQQQQQQQ QQQQQQQQQQ RFSYPGMHQT HLKEPNEQMA RNPNPSSTPL SNTPLSPVKN 370 380 390 400 410 420 SISGQTGVSS LKPGPLPPNL DDLKVSELRQ QLRIRGLPVS GTKTALVDRL RPFQDCAGNP 430 440 450 460 470 480 VPNFGDITTV TFPVTPNTLP SYQSSPTGFY HFGSTSSSPP ISPASSDLSA AGSLPDTFTD 490 500 510 520 530 540 ASPGFGLHAS PVPACTDESL LSSLNGGSGP SEPDGLDSEK DKMLVEKQKV INQLTWKLRQ 550 560 570 580 590 600 EQRQVEELRM QLQKQKSSCS DQKPLPFLAT TIKQEDVSSC PFAPQQASGK GQGHSSDSPP 610 620 630 640 650 660 PACETAQLLP HCVESSGQTH VLSSTFLSPQ CSPQHSPLGG LKSPQHISLP PSPNNHYFLA 670 680 690 700 710 720 SSSGAQRENH GVSSPSSSQG CAQNSGAHEG HSSSFSSPAS SLHQPFSGTQ ADSSHSAGLN 730 740 750 760 770 780 PCPKSPSIHP KMTGLQSSDK VGPTFSIPSP TFSKSSSAVS DITQPPSYED AVKQQMTRSQ 790 800 810 820 830 840 QMDELLDVLI ESGEMPADAR EDHSCLQKIP KIPGSSCSPT AIPPKPSASF EQASSGGQMA 850 860 870 880 890 900 FDHYANDSDE HLEVLLNSHS PIGKVSDVTL LKIGSEEPPF DSIMDGFPGK AAEDLFSAHE 910 920 930 940 950 960 LLPGPLSPMH AQLSPPSVDS SGLQLSFTES PWETMEWLDL TPPSSTPGFS NLTSSGPSIF 970 980 NIDFLDVTDL NLNSPMDLHL QQW
	Isoform 4 (MYOCD-v3) [UniParc]. Checksum: 535A0D43433EEDD7	FASTA	904	97,066
	10 20 30 40 50 60 MHILQASTAE RSIPTAQMKL KRARLADDLN EKIALRPGPL ELVEKNILPM DSSVKEAIKG 70 80 90 100 110 120 TEVSLSKAAD AFAFEDDSSR DGLSPDQARS EDPQGSTGST PDIKSTEAPL DTIQDLTPGS 130 140 150 160 170 180 ESDKNDAASQ PGNQSDPGKQ VLGPLSTPIP VHTAVKSKSL GDSKNRHKKP KDPKPKVKKL 190 200 210 220 230 240 KYHQYIPPDQ KAEKSPPPMD SAYARLLQQQ QLFLQLQILS QQQQQQQQQQ QQQQQQQQQQ 250 260 270 280 290 300 QRFSYPGMHQ THLKEPNEQM ARNPNPSSTP LSNTPLSPVK NSISGQTGVS SLKPGPLPPN 310 320 330 340 350 360 LDDLKVSELR QQLRIRGLPV SGTKTALVDR LRPFQDCAGN PVPNFGDITT VTFPVTPNTL 370 380 390 400 410 420 PSYQSSPTGF YHFGSTSSSP PISPASSDLS AAGSLPDTFT DASPGFGLHA SPVPACTDES 430 440 450 460 470 480 LLSSLNGGSG PSEPDGLDSE KDKMLVEKQK VINQLTWKLR QEQRQVEELR MQLQKQKSSC 490 500 510 520 530 540 SDQKPLPFLA TTIKQEDVSS CPFAPQQASG KGQGHSSDSP PPACETAQLL PHCVESSGQT 550 560 570 580 590 600 HVLSSTFLSP QCSPQHSPLG GLKSPQHISL PPSPNNHYFL ASSSGAQREN HGVSSPSSSQ 610 620 630 640 650 660 GCAQNSGAHE GHSSSFSSPA SSLHQPFSGT QADSSHSAGL NPCPKSPSIH PKMTGLQSSD 670 680 690 700 710 720 KVGPTFSIPS PTFSKSSSAV SDITQPPSYE DAVKQQMTRS QQMDELLDVL IESGEMPADA 730 740 750 760 770 780 REDHSCLQKI PKIPGSSCSP TAIPPKPSAS FEQASSGGQM AFDHYANDSD EHLEVLLNSH 790 800 810 820 830 840 SPIGKVSDVT LLKIGSEEPP FDSIMDGFPG KAAEDLFSAH ELLPGPLSPM HAQLSPPSVD 850 860 870 880 890 900 SSGLQLSFTE SPWETMEWLD LTPPSSTPGF SNLTSSGPSI FNIDFLDVTD LNLNSPMDLH LQQW
	Isoform 5 (MYOCD-v4) [UniParc]. Checksum: D2243E5464F1297D	FASTA	856	92,243
	10 20 30 40 50 60 MHILQASTAE RSIPTAQMKL KRARLADDLN EKIALRPGPL ELVEKNILPM DSSVKEAIKG 70 80 90 100 110 120 TEVSLSKAAD AFAFEDDSSR DGLSPDQARS EDPQGSTGST PDIKSTEAPL DTIQDLTPGS 130 140 150 160 170 180 ESDKNDAASQ PGNQSDPGKQ VLGPLSTPIP VHTAVKSKSL GDSKNRHKKP KDPKPKVKKL 190 200 210 220 230 240 KYHQYIPPDQ KAEKSPPPMD SAYARLLQQQ QLFLQLQILS QQQQQQQQQQ QQQQQQQQQQ 250 260 270 280 290 300 QRFSYPGMHQ THLKEPNEQM ARNPNPSSTP LSNTPLSPVK NSISGQTGVS SLKPGPLPPN 310 320 330 340 350 360 LDDLKVSELR QQLRIRGLPV SGTKTALVDR LRPFQDCAGN PVPNFGDITT VTFPVTPNTL 370 380 390 400 410 420 PSYQSSPTGF YHFGSTSSSP PISPASSDLS AAGSLPDTFT DASPGFGLHA SPVPACTDES 430 440 450 460 470 480 LLSSLNGGSG PSEPDGLDSE KDKMLVEKQK VINQLTWKLR QEQRQVEELR MQLQKQKSSC 490 500 510 520 530 540 SDQKPLPFLA TTIKQEDVSS CPFAPQQASG KGQGHSSDSP PPACETAQLL PHCVESSGQT 550 560 570 580 590 600 HVLSSTFLSP QCSPQHSPLG GLKSPQHISL PPSPNNHYFL ASSSGAQREN HGVSSPSSSQ 610 620 630 640 650 660 GCAQMTGLQS SDKVGPTFSI PSPTFSKSSS AVSDITQPPS YEDAVKQQMT RSQQMDELLD 670 680 690 700 710 720 VLIESGEMPA DAREDHSCLQ KIPKIPGSSC SPTAIPPKPS ASFEQASSGG QMAFDHYAND 730 740 750 760 770 780 SDEHLEVLLN SHSPIGKVSD VTLLKIGSEE PPFDSIMDGF PGKAAEDLFS AHELLPGPLS 790 800 810 820 830 840 PMHAQLSPPS VDSSGLQLSF TESPWETMEW LDLTPPSSTP GFSNLTSSGP SIFNIDFLDV 850 TDLNLNSPMD LHLQQW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor." Wang D.-Z., Chang P.S., Wang Z., Sutherland L., Richardson J.A., Small E., Krieg P.A., Olson E.N. Cell 105:851-862(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INTERACTION WITH SRF, MUTAGENESIS OF 387-GLU--ARG-389 AND 408-ASP--LEU-410. Tissue: Heart.
[2]	"Potentiation of serum response factor activity by a family of myocardin-related transcription factors." Wang D.-Z., Li S., Hockemeyer D., Sutherland L., Wang Z., Schratt G., Richardson J.A., Nordheim A., Olson E.N. Proc. Natl. Acad. Sci. U.S.A. 99:14855-14860(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SEQUENCE REVISION TO 1-128.
[3]	"Myocardin expression is regulated by Nkx2.5, and its function is required for cardiomyogenesis." Ueyama T., Kasahara H., Ishiwata T., Nie Q., Izumo S. Mol. Cell. Biol. 23:9222-9232(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Strain: NIH Swiss.
[4]	"An alternative splicing form of myocardin (BSAC2), myocardin A (BSAC2A)." Sawada T., Okazaki T., Nakano H. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Heart.
[5]	"Myocardin is a critical serum response factor cofactor in the transcriptional program regulating smooth muscle cell differentiation." Du K.L., Ip H.S., Li J., Chen M., Dandre F., Yu W., Lu M.M., Owens G.K., Parmacek M.S. Mol. Cell. Biol. 23:2425-2437(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryonic heart.
[7]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[8]	"Myocardin is a key regulator of CArG-dependent transcription of multiple smooth muscle marker genes." Yoshida T., Sinha S., Dandre F., Wamhoff B.R., Hoofnagle M.H., Kremer B.E., Wang D.-Z., Olson E.N., Owens G.K. Circ. Res. 92:856-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SRF.
[9]	"Glycogen synthase kinase 3beta inhibits myocardin-dependent transcription and hypertrophy induction through site-specific phosphorylation." Badorff C., Seeger F.H., Zeiher A.M., Dimmeler S. Circ. Res. 97:645-654(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-454; SER-458; SER-462; SER-466: SER-624; SER-628; SER-632 AND SER-636.
[10]	"Modulation of smooth muscle gene expression by association of histone acetyltransferases and deacetylases with myocardin." Cao D., Wang Z., Zhang C.L., Oh J., Xing W., Li S., Richardson J.A., Wang D.Z., Olson E.N. Mol. Cell. Biol. 25:364-376(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH EP300; HDAC4 AND HDAC5.
[11]	"Coactivation of MEF2 by the SAP domain proteins myocardin and MASTR." Creemers E.E., Sutherland L.B., Oh J., Barbosa A.C., Olson E.N. Mol. Cell 23:83-96(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 2 AND 4), INTERACTION WITH MEF2C, FUNCTION.
[12]	"Phosphorylation of myocardin by extracellular signal-regulated kinase." Taurin S., Sandbo N., Yau D.M., Sethakorn N., Kach J., Dulin N.O. J. Biol. Chem. 284:33789-33794(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-812; SER-859; SER-866 AND THR-893, MUTAGENESIS OF SER-812; SER-859; SER-866 AND THR-893.
[13]	"Expression and functional activity of four myocardin isoforms." Imamura M., Long X., Nanda V., Miano J.M. Gene 464:1-10(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3; 4 AND 5), TISSUE SPECIFICITY OF ISOFORMS, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF384055 mRNA. Translation: AAK71683.2. AY303755 mRNA. Translation: AAQ63841.1. AF437877 mRNA. Translation: AAL30892.1. AK084700 mRNA. Translation: BAC39258.1. AK142216 mRNA. Translation: BAE24980.1. AL669846 Genomic DNA. Translation: CAI25017.1.
IPI	IPI00331360. IPI00876570. IPI00975097. IPI01027112. IPI01027190.
RefSeq	NP_660118.3. NM_145136.4. NP_666498.2. NM_146386.3.
UniGene	Mm.32257.
3D structure databases
ProteinModelPortal	Q8VIM5.
SMR	Q8VIM5. Positions 17-129, 372-422.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29754N.
PTM databases
PhosphoSite	Q8VIM5.
Proteomic databases
PaxDb	Q8VIM5.
PRIDE	Q8VIM5.
Protocols and materials databases
DNASU	214384.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000101042; ENSMUSP00000098603; ENSMUSG00000020542. ENSMUST00000102635; ENSMUSP00000099695; ENSMUSG00000020542. ENSMUST00000108695; ENSMUSP00000104335; ENSMUSG00000020542.
GeneID	214384.
KEGG	mmu:214384.
Organism-specific databases
CTD	93649.
MGI	MGI:2137495. Myocd.
Phylogenomic databases
eggNOG	NOG79803.
GeneTree	ENSGT00530000063195.
HOGENOM	HOG000038001.
HOVERGEN	HBG036493.
InParanoid	Q6W8X1.
OMA	SFTESPW.
OrthoDB	EOG4640BZ.
Gene expression databases
ArrayExpress	Q8VIM5.
Bgee	Q8VIM5.
CleanEx	MM_MYOCD.
Genevestigator	Q8VIM5.
GermOnline	ENSMUSG00000020542. Mus musculus.
Family and domain databases
Gene3D	1.10.720.30. 1 hit.
InterPro	IPR004018. RPEL_repeat. IPR003034. SAP_dom. [Graphical view]
Pfam	PF02755. RPEL. 1 hit. PF02037. SAP. 1 hit. [Graphical view]
SMART	SM00707. RPEL. 3 hits. SM00513. SAP. 1 hit. [Graphical view]
PROSITE	PS51073. RPEL. 3 hits. PS50800. SAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	374288.
SOURCE	Search...

Entry information

Entry name

MYCD_MOUSE

Accession

Primary (citable) accession number: Q8VIM5
Secondary accession number(s): Q5SS65 , Q6W8X1, Q8C3W6, Q8VIL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program