Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myocardin

Gene

Myocd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). Isoform 1 mediates the cardiac transcription factor MEF2C-dependent transcription. Isoform 1 and isoform 3 are more active than isoform 2 and isoform 4 in stimulating cardiac muscle promoters.5 Publications

GO - Molecular functioni

GO - Biological processi

  • cardiac muscle cell differentiation Source: BHF-UCL
  • cardiac ventricle development Source: MGI
  • cellular component maintenance Source: MGI
  • digestive tract development Source: MGI
  • ductus arteriosus closure Source: MGI
  • heart development Source: MGI
  • lung alveolus development Source: MGI
  • muscle cell differentiation Source: MGI
  • negative regulation of beta-amyloid clearance Source: MGI
  • negative regulation of cardiac muscle cell apoptotic process Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • negative regulation of myotube differentiation Source: MGI
  • negative regulation of skeletal muscle cell differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of cardiac muscle cell differentiation Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of DNA binding Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • positive regulation of smooth muscle cell differentiation Source: MGI
  • positive regulation of smooth muscle contraction Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation Source: MGI
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
  • regulation of cell growth by extracellular stimulus Source: MGI
  • regulation of histone acetylation Source: UniProtKB
  • regulation of myoblast differentiation Source: MGI
  • regulation of phenotypic switching Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to hypoxia Source: Ensembl
  • smooth muscle cell differentiation Source: MGI
  • urinary bladder development Source: MGI
  • uterus development Source: MGI
  • vascular smooth muscle cell differentiation Source: BHF-UCL
  • vasculogenesis Source: MGI
  • ventricular cardiac muscle cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Myocardin
Alternative name(s):
Basic SAP coiled-coil transcription activator 2
SRF cofactor protein
Gene namesi
Name:Myocd
Synonyms:Bsac2, Mycd, Srfcp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2137495. Myocd.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
  • SRF-myogenin-E12 complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi387 – 389ELR → PSF: Activation of ANF promoter abolished, no effect on SM22 promoter. 1 Publication3
Mutagenesisi408 – 410DRL → PGH: Activation of ANF promoter abolished, no effect on SM22 promoter. 1 Publication3
Mutagenesisi812S → A: No effect on SRF activation. No effect on SRF activation, on interaction with EP300 and on SM-promoter activation; when associated with A-859; A-866 and A-893. 1 Publication1
Mutagenesisi812S → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-859; D-866 and D-893. 1 Publication1
Mutagenesisi859S → A: No effect on SRF activation; No effect on SRF activation. No effect on SRF activation, on interaction with EP300 and on SM-promoter activation; when associated with A-812, A-866 and A-893. 1 Publication1
Mutagenesisi859S → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-812; D-866 and D-893. 1 Publication1
Mutagenesisi866S → A: No effect on SRF activation; No effect on SRF activation, on interaction with EP300 and on SM-promoter activation; when associated with A-812; A-859 and A-893. 1 Publication1
Mutagenesisi866S → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-812; D-859 and D-893. 1 Publication1
Mutagenesisi893T → A: No effect on SRF activation; No effect on SRF activation, on interaction with EP300 and on SM-specific genes transcription; when associated with A-812; A-859 and A-866. 1 Publication1
Mutagenesisi893T → D: No effect on SRF activation. Impairs SRF activation, reduces interaction with EP300 and SM-promoter activation; when associated with D-812; D-859 and D-866. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266321 – 935MyocardinAdd BLAST935

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei454Phosphoserine; by GSK3-beta1 Publication1
Modified residuei458Phosphoserine; by GSK3-beta1 Publication1
Modified residuei462Phosphoserine; by GSK3-beta1 Publication1
Modified residuei466Phosphoserine; by GSK3-beta1 Publication1
Modified residuei624Phosphoserine; by GSK3-beta1 Publication1
Modified residuei628Phosphoserine; by GSK3-beta1 Publication1
Modified residuei632Phosphoserine; by GSK3-beta1 Publication1
Modified residuei636Phosphoserine; by GSK3-beta1 Publication1
Modified residuei812Phosphoserine; by MAPK1 AND MAPK31 Publication1
Modified residuei859Phosphoserine; by MAPK1 and MAPK31 Publication1
Modified residuei866Phosphoserine; by MAPK1 and MAPK31 Publication1
Modified residuei893Phosphothreonine; by MAPK1 and MAPK31 Publication1

Post-translational modificationi

Phosphorylation regulates negatively transcriptional activity.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VIM5.
PRIDEiQ8VIM5.

PTM databases

iPTMnetiQ8VIM5.
PhosphoSitePlusiQ8VIM5.

Expressioni

Tissue specificityi

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder and uterus. Isoform 1 and isoform 3 are predominantly expressed in cardiac muscle whereas isoform 4 and isoform 5 are predominantly expressed in SMC-rich tissues. Isoform 3 is the most abundant isoform in the heart from embryo to adult.5 Publications

Developmental stagei

Detected in the cardiac crescent at 7.75 dpc and in the linear heart tube at 8.0 dpc and the developing atrial and aortic ventricular chambers until birth. Also detected in a subset of vascular and visceral smooth muscle cells: aortic arch arteries at 9.5 dpc; walls of the esophagus, dorsal aorta, pulmonary outflow tract, lung, gut, stomach, small intestine, bladder, and the head mesenchyme at 13.5 dpc until birth. Not detected in skeletal muscle cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000020542.
CleanExiMM_MYOCD.
ExpressionAtlasiQ8VIM5. baseline and differential.
GenevisibleiQ8VIM5. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with MLLT7/FOXO4 (By similarity). Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation. Interacts (via C-terminal) with EP300 (via CREB-binding domain). Interacts with HDAC4 and HDAC5. Interacts with MEF2C.By similarity4 Publications

GO - Molecular functioni

  • histone acetyltransferase binding Source: MGI
  • histone deacetylase binding Source: MGI
  • R-SMAD binding Source: BHF-UCL
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi229522. 15 interactors.
DIPiDIP-29754N.
STRINGi10090.ENSMUSP00000104335.

Structurei

3D structure databases

ProteinModelPortaliQ8VIM5.
SMRiQ8VIM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 43RPEL 1Add BLAST26
Repeati62 – 87RPEL 2Add BLAST26
Repeati106 – 131RPEL 3Add BLAST26
Domaini380 – 414SAPPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 201HDAC5-bindingAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili287 – 322Sequence analysisAdd BLAST36
Coiled coili519 – 563Sequence analysisAdd BLAST45

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 27MEF2C-bindingAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi287 – 320Gln-richAdd BLAST34
Compositional biasi300 – 320Poly-GlnAdd BLAST21

Domaini

The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.

Sequence similaritiesi

Contains 3 RPEL repeats.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IE2C. Eukaryota.
ENOG410XNR1. LUCA.
GeneTreeiENSGT00530000063195.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiQ8VIM5.
OMAiLVNMHIL.
OrthoDBiEOG091G02RT.
TreeFamiTF326024.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR028721. MYOCD.
IPR004018. RPEL_repeat.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR22793:SF11. PTHR22793:SF11. 2 hits.
PfamiPF02755. RPEL. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VIM5-1) [UniParc]FASTAAdd to basket
Also known as: MYOCD-v2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLLGSEHSL LIRRKFRSVL QLRLQQRRTQ EQLANQGLIP PLKGPTEFHD
60 70 80 90 100
PRKQLDSAKT EDSLRRKGRN RSDRASLVTM HILQASTAER SIPTAQMKLK
110 120 130 140 150
RARLADDLNE KIALRPGPLE LVEKNILPMD SSVKEAIKGT EVSLSKAADA
160 170 180 190 200
FAFEDDSSRD GLSPDQARSE DPQGSTGSTP DIKSTEAPLD TIQDLTPGSE
210 220 230 240 250
SDKNDAASQP GNQSDPGKQV LGPLSTPIPV HTAVKSKSLG DSKNRHKKPK
260 270 280 290 300
DPKPKVKKLK YHQYIPPDQK AEKSPPPMDS AYARLLQQQQ LFLQLQILSQ
310 320 330 340 350
QQQQQQQQQQ QQQQQQQQQQ RFSYPGMHQT HLKEPNEQMA RNPNPSSTPL
360 370 380 390 400
SNTPLSPVKN SISGQTGVSS LKPGPLPPNL DDLKVSELRQ QLRIRGLPVS
410 420 430 440 450
GTKTALVDRL RPFQDCAGNP VPNFGDITTV TFPVTPNTLP SYQSSPTGFY
460 470 480 490 500
HFGSTSSSPP ISPASSDLSA AGSLPDTFTD ASPGFGLHAS PVPACTDESL
510 520 530 540 550
LSSLNGGSGP SEPDGLDSEK DKMLVEKQKV INQLTWKLRQ EQRQVEELRM
560 570 580 590 600
QLQKQKSSCS DQKPLPFLAT TIKQEDVSSC PFAPQQASGK GQGHSSDSPP
610 620 630 640 650
PACETAQLLP HCVESSGQTH VLSSTFLSPQ CSPQHSPLGG LKSPQHISLP
660 670 680 690 700
PSPNNHYFLA SSSGAQRENH GVSSPSSSQG CAQMTGLQSS DKVGPTFSIP
710 720 730 740 750
SPTFSKSSSA VSDITQPPSY EDAVKQQMTR SQQMDELLDV LIESGEMPAD
760 770 780 790 800
AREDHSCLQK IPKIPGSSCS PTAIPPKPSA SFEQASSGGQ MAFDHYANDS
810 820 830 840 850
DEHLEVLLNS HSPIGKVSDV TLLKIGSEEP PFDSIMDGFP GKAAEDLFSA
860 870 880 890 900
HELLPGPLSP MHAQLSPPSV DSSGLQLSFT ESPWETMEWL DLTPPSSTPG
910 920 930
FSNLTSSGPS IFNIDFLDVT DLNLNSPMDL HLQQW
Length:935
Mass (Da):101,400
Last modified:July 27, 2011 - v2
Checksum:iA8FCFC68A923A5CB
GO
Isoform 2 (identifier: Q8VIM5-2) [UniParc]FASTAAdd to basket
Also known as: BSAC2A, Myocardin A, MYOCD-v5

The sequence of this isoform differs from the canonical sequence as follows:
     1-128: Missing.
     683-683: Q → QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPK

Note: No experimental confirmation available.
Show »
Length:855
Mass (Da):91,592
Checksum:i73C3AAB62E61DB33
GO
Isoform 3 (identifier: Q8VIM5-3) [UniParc]FASTAAdd to basket
Also known as: =Myocardin A, MYOCD-v1

The sequence of this isoform differs from the canonical sequence as follows:
     683-683: Q → QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPK

Show »
Length:983
Mass (Da):106,223
Checksum:i7640D2863F9DB517
GO
Isoform 4 (identifier: Q8VIM5-4) [UniParc]FASTAAdd to basket
Also known as: MYOCD-v3

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     683-683: Q → QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPK

Show »
Length:904
Mass (Da):97,066
Checksum:i535A0D43433EEDD7
GO
Isoform 5 (identifier: Q8VIM5-5) [UniParc]FASTAAdd to basket
Also known as: MYOCD-v4

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Show »
Length:856
Mass (Da):92,243
Checksum:iD2243E5464F1297D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116P → Q in AAK71683 (PubMed:11439182).Curated1
Sequence conflicti794D → G in AAK71683 (PubMed:11439182).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0076621 – 128Missing in isoform 2. 1 PublicationAdd BLAST128
Alternative sequenceiVSP_0416841 – 79Missing in isoform 4 and isoform 5. CuratedAdd BLAST79
Alternative sequenceiVSP_007663683Q → QNSGAHEGHSSSFSSPASSL HQPFSGTQADSSHSAGLNPC PKSPSIHPK in isoform 2, isoform 3 and isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384055 mRNA. Translation: AAK71683.2.
AY303755 mRNA. Translation: AAQ63841.1.
AF437877 mRNA. Translation: AAL30892.1.
AK084700 mRNA. Translation: BAC39258.1.
AK142216 mRNA. Translation: BAE24980.1.
AL669846 Genomic DNA. Translation: CAI25017.1.
CCDSiCCDS24843.1. [Q8VIM5-1]
CCDS48819.1. [Q8VIM5-3]
RefSeqiNP_660118.3. NM_145136.4. [Q8VIM5-3]
NP_666498.2. NM_146386.3. [Q8VIM5-1]
XP_011247197.1. XM_011248895.1. [Q8VIM5-4]
UniGeneiMm.32257.

Genome annotation databases

EnsembliENSMUST00000101042; ENSMUSP00000098603; ENSMUSG00000020542. [Q8VIM5-2]
ENSMUST00000102635; ENSMUSP00000099695; ENSMUSG00000020542. [Q8VIM5-1]
ENSMUST00000108695; ENSMUSP00000104335; ENSMUSG00000020542. [Q8VIM5-3]
GeneIDi214384.
KEGGimmu:214384.
UCSCiuc007jky.2. mouse. [Q8VIM5-3]
uc007jkz.2. mouse. [Q8VIM5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384055 mRNA. Translation: AAK71683.2.
AY303755 mRNA. Translation: AAQ63841.1.
AF437877 mRNA. Translation: AAL30892.1.
AK084700 mRNA. Translation: BAC39258.1.
AK142216 mRNA. Translation: BAE24980.1.
AL669846 Genomic DNA. Translation: CAI25017.1.
CCDSiCCDS24843.1. [Q8VIM5-1]
CCDS48819.1. [Q8VIM5-3]
RefSeqiNP_660118.3. NM_145136.4. [Q8VIM5-3]
NP_666498.2. NM_146386.3. [Q8VIM5-1]
XP_011247197.1. XM_011248895.1. [Q8VIM5-4]
UniGeneiMm.32257.

3D structure databases

ProteinModelPortaliQ8VIM5.
SMRiQ8VIM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229522. 15 interactors.
DIPiDIP-29754N.
STRINGi10090.ENSMUSP00000104335.

PTM databases

iPTMnetiQ8VIM5.
PhosphoSitePlusiQ8VIM5.

Proteomic databases

PaxDbiQ8VIM5.
PRIDEiQ8VIM5.

Protocols and materials databases

DNASUi214384.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101042; ENSMUSP00000098603; ENSMUSG00000020542. [Q8VIM5-2]
ENSMUST00000102635; ENSMUSP00000099695; ENSMUSG00000020542. [Q8VIM5-1]
ENSMUST00000108695; ENSMUSP00000104335; ENSMUSG00000020542. [Q8VIM5-3]
GeneIDi214384.
KEGGimmu:214384.
UCSCiuc007jky.2. mouse. [Q8VIM5-3]
uc007jkz.2. mouse. [Q8VIM5-1]

Organism-specific databases

CTDi93649.
MGIiMGI:2137495. Myocd.

Phylogenomic databases

eggNOGiENOG410IE2C. Eukaryota.
ENOG410XNR1. LUCA.
GeneTreeiENSGT00530000063195.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiQ8VIM5.
OMAiLVNMHIL.
OrthoDBiEOG091G02RT.
TreeFamiTF326024.

Miscellaneous databases

PROiQ8VIM5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020542.
CleanExiMM_MYOCD.
ExpressionAtlasiQ8VIM5. baseline and differential.
GenevisibleiQ8VIM5. MM.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR028721. MYOCD.
IPR004018. RPEL_repeat.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR22793:SF11. PTHR22793:SF11. 2 hits.
PfamiPF02755. RPEL. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYCD_MOUSE
AccessioniPrimary (citable) accession number: Q8VIM5
Secondary accession number(s): Q5SS65
, Q6W8X1, Q8C3W6, Q8VIL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.