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Q8VIM4

- BSND_MOUSE

UniProt

Q8VIM4 - BSND_MOUSE

Protein

Barttin

Gene

Bsnd

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 3 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter.

    GO - Molecular functioni

    1. chloride channel regulator activity Source: Ensembl
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular chloride ion homeostasis Source: UniProtKB
    2. cellular ion homeostasis Source: MGI
    3. cellular potassium ion homeostasis Source: UniProtKB
    4. chloride transmembrane transport Source: GOC
    5. sensory perception of sound Source: UniProtKB

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Barttin
    Gene namesi
    Name:Bsnd
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2153465. Bsnd.

    Subcellular locationi

    Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Staining in membranes of the renal tubule is basolateral. Also detected in basolateral membranes of intercalated cells of the collecting duct, which are known to express CLCNKB as well. Both acid-secreting alpha-intercalated cells and base-secreting beta-intercalated cells express this protein basolaterally, but intervening AQP2-expressing principal cells appear devoid of protein expression. In the inner ear, colocalizes with CLCNK in K+-secreting marginal cells of the stria vascularis. The basolateral staining contrasts with the apical localization of the KCNQ1 K+ channel. Also found in K+-secreting vestibular dark cells, where it colocalized in basolateral membranes with CLCNK below apical membranes that expressed KCNQ1.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. integral component of membrane Source: MGI
    3. integral component of plasma membrane Source: UniProtKB
    4. protein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307BarttinPRO_0000065000Add
    BLAST

    Proteomic databases

    MaxQBiQ8VIM4.
    PaxDbiQ8VIM4.
    PRIDEiQ8VIM4.

    PTM databases

    PhosphoSiteiQ8VIM4.

    Expressioni

    Tissue specificityi

    Expression is evident in inner and outer stripes of the outer medulla of the kidney, most probably representing thin limbs of Henle's loop together with some collecting duct coursing through the outer stripe. In situ hybridization in fetal kidney at 18.5 dpc revealed a clear continuity between hybridization signals from the thin limb of Henle's loop and the distal convoluted tubule, suggesting that part of the expression pattern may result from expression in the thick ascending limb of Henle's loop. In addition, strong signals are present in a subset of cortical tubules, representing distal convoluted tubules or cortical collecting duct. Strong expression is also observed in the inner medulla of the kidney. This expression does not extend all the way to the tip of the papilla. Thus this signal most probably represents cells of the thin ascending limbs. In the inner ear, strong and exclusive expression is detected in marginal cells of the stria vascularis. In addition to cochlear signal, expression is observed in dark cells localized at the base of the crista ampullaris of the vestibular organ.2 Publications

    Gene expression databases

    GenevestigatoriQ8VIM4.

    Interactioni

    Subunit structurei

    Interacts with CLCNK channels. Forms probably heteromers with CLCNKA in the thin ascending limb of Henle and with CLCNKB in the thick ascending limb and more distal segments.1 Publication

    Protein-protein interaction databases

    IntActiQ8VIM4. 1 interaction.
    MINTiMINT-4089408.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VIM4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 55CytoplasmicSequence Analysis
    Topological domaini27 – 326ExtracellularSequence Analysis
    Topological domaini54 – 307254CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei33 – 5321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45959.
    GeneTreeiENSGT00390000008549.
    HOGENOMiHOG000049268.
    HOVERGENiHBG050739.
    InParanoidiQ8VIM4.
    OMAiCYPKITF.
    OrthoDBiEOG7BS4BK.
    TreeFamiTF335975.

    Family and domain databases

    InterProiIPR029181. Barttin_dom.
    [Graphical view]
    PfamiPF15462. Barttin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VIM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADEKTFRIG FIVLGLFLLS LGTFLMSHDR PQVYGTFYAM GSVMVIGGVI    50
    WSMCQCYPKI TFVPADSDFQ GILSPKALSL LETGLSEVKS PQPPYVRLWE 100
    EAAYDQSLPD FTHIQMKVMG YSEDPRPLLA PELKTGASSV REGEPRTAQA 150
    WMEAPVVVHR GSDENEGEKS HSQSSPSVGP QGSAPLASFH DDLDVGSSEG 200
    SSLQPSPNRD EPHRQVPWAS RGPLDRFSDF ALIDDTPTSE DTVLDGQARE 250
    AALPRKQQWS LRMKGETVQA RAEEPEQEEE DLYYGLPDSP GNPLPDKELG 300
    FEPDIQG 307
    Length:307
    Mass (Da):33,813
    Last modified:October 3, 2012 - v3
    Checksum:iBB837B92EC39F2CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti83 – 831T → A in AAL33907. (PubMed:11687798)Curated
    Sequence conflicti292 – 2921N → D in AAQ81629. 1 PublicationCurated
    Sequence conflicti292 – 2921N → D in AAH38287. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF391088 mRNA. Translation: AAL33907.1.
    AY373833 mRNA. Translation: AAQ81629.1.
    AK052587 mRNA. Translation: BAC35049.1.
    AL954352 Genomic DNA. Translation: CAM15752.1.
    BC038287 mRNA. Translation: AAH38287.1.
    CCDSiCCDS18419.1.
    RefSeqiNP_536706.2. NM_080458.2.
    UniGeneiMm.135448.

    Genome annotation databases

    EnsembliENSMUST00000054472; ENSMUSP00000049563; ENSMUSG00000025418.
    GeneIDi140475.
    KEGGimmu:140475.
    UCSCiuc008tyj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF391088 mRNA. Translation: AAL33907.1 .
    AY373833 mRNA. Translation: AAQ81629.1 .
    AK052587 mRNA. Translation: BAC35049.1 .
    AL954352 Genomic DNA. Translation: CAM15752.1 .
    BC038287 mRNA. Translation: AAH38287.1 .
    CCDSi CCDS18419.1.
    RefSeqi NP_536706.2. NM_080458.2.
    UniGenei Mm.135448.

    3D structure databases

    ProteinModelPortali Q8VIM4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VIM4. 1 interaction.
    MINTi MINT-4089408.

    PTM databases

    PhosphoSitei Q8VIM4.

    Proteomic databases

    MaxQBi Q8VIM4.
    PaxDbi Q8VIM4.
    PRIDEi Q8VIM4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054472 ; ENSMUSP00000049563 ; ENSMUSG00000025418 .
    GeneIDi 140475.
    KEGGi mmu:140475.
    UCSCi uc008tyj.1. mouse.

    Organism-specific databases

    CTDi 7809.
    MGIi MGI:2153465. Bsnd.

    Phylogenomic databases

    eggNOGi NOG45959.
    GeneTreei ENSGT00390000008549.
    HOGENOMi HOG000049268.
    HOVERGENi HBG050739.
    InParanoidi Q8VIM4.
    OMAi CYPKITF.
    OrthoDBi EOG7BS4BK.
    TreeFami TF335975.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 369764.
    PROi Q8VIM4.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q8VIM4.

    Family and domain databases

    InterProi IPR029181. Barttin_dom.
    [Graphical view ]
    Pfami PF15462. Barttin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/6.
    2. "Functional phenotype of inner ear-specific chloride channel ClC-K and its accessory subunit."
      Nie L., Feng W., Dinglasan J.N., Yamoah E.N.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Cochlea.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    6. "Barttin is a Cl- channel beta-subunit crucial for renal Cl-reabsorption and inner ear K+ secretion."
      Estevez R., Boettger T., Stein V., Birkenhaeger R., Otto E., Hildebrandt F., Jentsch T.J.
      Nature 414:558-561(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiBSND_MOUSE
    AccessioniPrimary (citable) accession number: Q8VIM4
    Secondary accession number(s): B1AZI5, Q8C740, Q8CHY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 76 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3