Q8VIM0 (HAVR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hepatitis A virus cellular receptor 2 homolog
Short name=HAVcr-2

Alternative name(s):
T-cell immunoglobulin and mucin domain-containing protein 3
Short name=TIMD-3
T-cell membrane protein 3
TIM-3

Gene names

Name:	Havcr2
Synonyms:	Tim3, Timd3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9 By similarity. Ref.3
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed in T-helper type 1 lymphocytes. Not expressed by naive T-cells but up-regulated as they differentiate into T-helper-1 cells. Also expressed by differentiated type 1 CD8+ cytotoxic T-cells. Ref.3
Miscellaneous	Belongs to the T-cell and airway phenotype regulator (Tapr) locus, a single chromosomal region that confers reduced T-helper type 2 responsiveness and protects against airway hyperactivity (AHR), the hallmark of human asthma. In vivo administration of antibody to HAVCR2 enhances the clinical and pathological severity of experimental autoimmune encephalomyelitis (EAE), a Th1-dependent autoimmune disease and increases the number and activation level of macrophages.
Sequence similarities	Belongs to the immunoglobulin superfamily. TIM family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 281

262

Hepatitis A virus cellular receptor 2 homolog

PRO_0000042102

Regions

Topological domain

20 – 193

174

Extracellular Potential

Transmembrane

194 – 214

21

Helical; Potential

Topological domain

215 – 281

67

Cytoplasmic Potential

Domain

20 – 125

106

Ig-like V-type

Compositional bias

221 – 224

4

Poly-Lys

Amino acid modifications

Glycosylation

74

1

N-linked (GlcNAc...) Potential

Glycosylation

100

1

N-linked (GlcNAc...) Potential

Glycosylation

172

1

N-linked (GlcNAc...) Potential

Disulfide bond

38 ↔ 111

By similarity

Disulfide bond

52 ↔ 63

By similarity

Disulfide bond

58 ↔ 110

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

281

Helix Strand Turn

Details...

Beta strand

34 – 36

3

Beta strand

51 – 53

3

Beta strand

60 – 62

3

Beta strand

67 – 70

4

Beta strand

72 – 77

6

Beta strand

81 – 85

5

Beta strand

96 – 100

5

Helix

103 – 105

3

Sequences

	Sequence		Length	Mass (Da)
	Q8VIM0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C0349E4BD0E5761D	FASTA	281	30,934
	10 20 30 40 50 60 MFSGLTLNCV LLLLQLLLAR SLENAYVFEV GKNAYLPCSY TLSTPGALVP MCWGKGFCPW 70 80 90 100 110 120 SQCTNELLRT DERNVTYQKS SRYQLKGDLN KGDVSLIIKN VTLDDHGTYC CRIQFPGLMN 130 140 150 160 170 180 DKKLELKLDI KAAKVTPAQT AHGDSTTASP RTLTTERNGS ETQTLVTLHN NNGTKISTWA 190 200 210 220 230 240 DEIKDSGETI RTAIHIGVGV SAGLTLALII GVLILKWYSC KKKKLSSLSL ITLANLPPGG 250 260 270 280 LANAGAVRIR SEENIYTIEE NVYEVENSNE YYCYVNSQQP S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of Tapr (an airway hyperreactivity regulatory locus) and the linked Tim gene family." McIntire J.J., Umetsu S.E., Akbari O., Potter M., Kuchroo V.K., Barsh G.S., Freeman G.J., Umetsu D.T., DeKruyff R.H. Nat. Immunol. 2:1109-1116(2001) [PubMed: 11725301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2. Tissue: Spleen.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease." Monney L., Sabatos C.A., Gaglia J.L., Ryu A., Waldner H., Chernova T., Manning S., Greenfield E.A., Coyle A.J., Sobel R.A., Freeman G.J., Kuchroo V.K. Nature 415:536-541(2002) [PubMed: 11823861] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

TIMD-3

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF399831 mRNA. Translation: AAL35776.1.
AL669948 Genomic DNA. No translation available.

IPI

IPI00270293.

RefSeq

NP_599011.2. NM_134250.2.

UniGene

Mm.72168.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3KAA	X-ray	3.00	A/B	29-133	[»]

ProteinModelPortal

Q8VIM0.

SMR

Q8VIM0. Positions 22-172.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8VIM0.

PTM databases

PhosphoSite

Q8VIM0.

Proteomic databases

PRIDE

Q8VIM0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000020668; ENSMUSP00000020668; ENSMUSG00000020399.

GeneID

171285.

KEGG

mmu:171285.

UCSC

uc011xtp.1. mouse.

Organism-specific databases

CTD

84868.

MGI

MGI:2159682. Havcr2.

Phylogenomic databases

GeneTree

ENSGT00440000039800.

HOGENOM

HBG125079.

HOVERGEN

HBG098563.

InParanoid

Q8VIM0.

OMA

QNALLPC.

OrthoDB

EOG4MGS86.

PhylomeDB

Q8VIM0.

Gene expression databases

ArrayExpress

Q8VIM0.

Bgee

Q8VIM0.

CleanEx

MM_HAVCR2.

Genevestigator

Q8VIM0.

GermOnline

ENSMUSG00000020399. Mus musculus.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

370963.

SOURCE

Search...

Entry information

Entry name

HAVR2_MOUSE

Accession

Primary (citable) accession number: Q8VIM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2002
Last modified:	September 21, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program