Reviewed, UniProtKB/Swiss-Prot Q8VIM0 (TIMD3_MOUSE)

Names and origin

Protein names

Recommended name:
    Hepatitis A virus cellular receptor 2 homolog
      Short name=HAVcr-2
Alternative name(s):
    T-cell immunoglobulin and mucin domain-containing protein 3
      Short name=TIMD-3
    T-cell membrane protein 3
    TIM-3

Gene names

Name:	Havcr2
Synonyms:	Tim3, Timd3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed in T-helper type 1 lymphocytes. Not expressed by naive T-cells but up-regulated as they differentiate into T-helper-1 cells. Also expressed by differentiated type 1 CD8+ cytotoxic T-cells. Ref.3
Miscellaneous	Belongs to the T-cell and airway phenotype regulator (Tapr) locus, a single chromosomal region that confers reduced T-helper type 2 responsiveness and protects against airway hyperactivity (AHR), the hallmark of human asthma. In vivo administration of antibody to HAVCR2 enhances the clinical and pathological severity of experimental autoimmune encephalomyelitis (EAE), a Th1-dependent autoimmune disease and increases the number and activation level of macrophages.
Sequence similarities	Belongs to the immunoglobulin superfamily. TIM family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 281	262	Hepatitis A virus cellular receptor 2 homolog		PRO_0000042102
Regions
	Topological domain	20 – 193	174	Extracellular Potential
	Transmembrane	194 – 214	21	Potential
	Topological domain	215 – 281	67	Cytoplasmic Potential
	Domain	20 – 125	106	Ig-like V-type
	Compositional bias	221 – 224	4	Poly-Lys
Amino acid modifications
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Disulfide bond	38 ↔ 111		By similarity
	Disulfide bond	52 ↔ 63		By similarity
	Disulfide bond	58 ↔ 110		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VIM0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C0349E4BD0E5761D	FASTA	281	30,934
	10 20 30 40 50 60 MFSGLTLNCV LLLLQLLLAR SLENAYVFEV GKNAYLPCSY TLSTPGALVP MCWGKGFCPW 70 80 90 100 110 120 SQCTNELLRT DERNVTYQKS SRYQLKGDLN KGDVSLIIKN VTLDDHGTYC CRIQFPGLMN 130 140 150 160 170 180 DKKLELKLDI KAAKVTPAQT AHGDSTTASP RTLTTERNGS ETQTLVTLHN NNGTKISTWA 190 200 210 220 230 240 DEIKDSGETI RTAIHIGVGV SAGLTLALII GVLILKWYSC KKKKLSSLSL ITLANLPPGG 250 260 270 280 LANAGAVRIR SEENIYTIEE NVYEVENSNE YYCYVNSQQP S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of Tapr (an airway hyperreactivity regulatory locus) and the linked Tim gene family." McIntire J.J., Umetsu S.E., Akbari O., Potter M., Kuchroo V.K., Barsh G.S., Freeman G.J., Umetsu D.T., DeKruyff R.H. Nat. Immunol. 2:1109-1116(2001) [PubMed: 11725301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2. Tissue: Spleen.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease." Monney L., Sabatos C.A., Gaglia J.L., Ryu A., Waldner H., Chernova T., Manning S., Greenfield E.A., Coyle A.J., Sobel R.A., Freeman G.J., Kuchroo V.K. Nature 415:536-541(2002) [PubMed: 11823861] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

TIMD-3

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF399831 mRNA. Translation: AAL35776.1. AL669948 Genomic DNA. No translation available.
IPI	IPI00270293.
RefSeq	NP_599011.2.
UniGene	Mm.72168
3D structure databases
SMR	Q8VIM0. Positions 24-131.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VIM0.
PTM databases
PhosphoSite	Q8VIM0.
Proteomic databases
PRIDE	Q8VIM0.
Genome annotation databases
Ensembl	ENSMUST00000020668; ENSMUSP00000020668; ENSMUSG00000020399; Mus musculus. [Genome view]
GeneID	171285.
KEGG	mmu:171285.
UCSC	uc007ion.1. mouse.
Organism-specific databases
CTD	171285.
MGI	MGI:2159682. Havcr2.
Phylogenomic databases
HOGENOM	Q8VIM0.
HOVERGEN	Q8VIM0.
OMA	PRMLTTK.
Gene expression databases
ArrayExpress	Q8VIM0.
Bgee	Q8VIM0.
CleanEx	MM_HAVCR2.
Genevestigator	Q8VIM0.
GermOnline	ENSMUSG00000020399. Mus musculus.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	370963.
SOURCE	Search...

Entry information

Entry name

TIMD3_MOUSE

Accession

Primary (citable) accession number: Q8VIM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)