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Q8VIJ5

- NCOAT_RAT

UniProt

Q8VIJ5 - NCOAT_RAT

Protein

Bifunctional protein NCOAT

Gene

Mgea5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Possesses hyaluronidase activity By similarity. Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3.By similarity2 Publications

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Enzyme regulationi

    Inhibited by Cu2+, Hg2+, Cd2+ and Zn2+ at 1 mM. Not inhibited by Co2+, Mg2+, Ca2+, Mn2+, Fe3+ and EDTA. Also inhibited by sodium chloride at 1M and 2-amino-2-hydroxymethyl-1,3-propanediol (trishydroxymethylaminomethane) at 75mM.1 Publication

    Kineticsi

    1. KM=2.55 mM for pNP-O-GlcNAc1 Publication

    pH dependencei

    Optimum pH is 6.4. Activity decreases sharply at pH below 5.0.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius. Less active at room temperature and shows very little activity at 4 degrees Celsius. Loses activity at 57 degrees Celsius within 5 minutes.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei175 – 1751Nucleophile; for O-GlcNAcase activityBy similarity
    Active sitei177 – 1771Proton donor; for O-GlcNAcase activityBy similarity

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC
    2. histone acetyltransferase activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. dATP metabolic process Source: RGD
    3. histone acetylation Source: GOC
    4. N-acetylglucosamine metabolic process Source: RGD
    5. necrotic cell death Source: RGD
    6. negative regulation of cardiac muscle adaptation Source: RGD
    7. negative regulation of protein glycosylation Source: RGD
    8. positive regulation of calcium ion transport Source: RGD
    9. positive regulation of calcium ion transport into cytosol Source: RGD
    10. positive regulation of cell killing Source: RGD
    11. positive regulation of DNA metabolic process Source: RGD
    12. positive regulation of glucose import Source: RGD
    13. positive regulation of growth hormone secretion Source: RGD
    14. positive regulation of insulin secretion Source: RGD
    15. positive regulation of mitochondrial depolarization Source: RGD
    16. positive regulation of protein complex disassembly Source: RGD
    17. positive regulation of proteolysis Source: RGD
    18. protein targeting to membrane Source: RGD
    19. response to steroid hormone Source: RGD

    Keywords - Molecular functioni

    Acyltransferase, Glycosidase, Hydrolase, Transferase

    Protein family/group databases

    CAZyiGH84. Glycoside Hydrolase Family 84.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein NCOAT
    Alternative name(s):
    Meningioma-expressed antigen 5
    Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
    Including the following 2 domains:
    Beta-hexosaminidase (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylhexosaminidase
    Hexosaminidase C
    N-acetyl-beta-D-glucosaminidase
    N-acetyl-beta-glucosaminidase
    O-GlcNAcase
    Histone acetyltransferase (EC:2.3.1.48)
    Short name:
    HAT
    Gene namesi
    Name:Mgea5
    Synonyms:Hexc, Mea5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi621077. Mgea5.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi853 – 8531D → N: Loss of histone acetyltransferase activity. 1 Publication
    Mutagenesisi884 – 8841D → N: Loss of histone acetyltransferase activity. 1 Publication
    Mutagenesisi891 – 8911Y → F: Loss of histone acetyltransferase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 916916Bifunctional protein NCOATPRO_0000252120Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei364 – 3641PhosphoserineBy similarity
    Disulfide bondi777 ↔ 793By similarity

    Post-translational modificationi

    Proteolytically cleaved by caspase-3.By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PaxDbiQ8VIJ5.
    PRIDEiQ8VIJ5.

    PTM databases

    PhosphoSiteiQ8VIJ5.

    Expressioni

    Tissue specificityi

    Ubiquitous. Expressed at highest levels in the brain and spleen.1 Publication

    Gene expression databases

    GenevestigatoriQ8VIJ5.

    Interactioni

    Subunit structurei

    Monomer. Binds both acetylated and unacetylated histone H4 tail but acetylation on 'Lys-8' of histone H4 abolishes binding. Interacts with CLOCK By similarity. According to PubMed:8034696 it is a heterodimer of an alpha and beta chain.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000024198.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VIJ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni583 – 916334Histone acetyltransferase activityAdd
    BLAST
    Regioni695 – 814120Required for histone H4 bindingBy similarityAdd
    BLAST

    Phylogenomic databases

    eggNOGiCOG0454.
    GeneTreeiENSGT00390000007726.
    HOGENOMiHOG000044964.
    HOVERGENiHBG053044.
    InParanoidiQ8VIJ5.
    KOiK15719.
    OMAiLIKVDIH.
    OrthoDBiEOG7P02H7.
    PhylomeDBiQ8VIJ5.

    Family and domain databases

    Gene3Di3.40.630.30. 2 hits.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR011496. Beta-N-acetylglucosaminidase.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF07555. NAGidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55729. SSF55729. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VIJ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVQKESQAAL EERESERNAN PASVSGASLE PSAAPAPGED NPSGAGAAAG    50
    TGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD 100
    YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE 150
    VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN 200
    EIYQYLGEPE TFLFCPTEYC GTFCYPSVSQ SPYLRTVGEK LLPGIEVLWT 250
    GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 300
    TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED 350
    STVSIQIKLE NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR 400
    QVAHSGAKTS VVDGTPLVAA PSLNATTVVT TVYQEPIMSQ GAALSGEPSA 450
    LTKEEEKKQP DEEPMDMVVE KQEESEHKSD NQILTEIVEA KMAEELKPMD 500
    TDKESIAESK SPEMSMQEDC INDIAPMQTD EQANKEQFVP GPNEKPLYAA 550
    EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD 600
    SEKIEEWRSR AAKFEEMCAL VMGMFTRLSN CANRTILYDM YSYVWDIKSI 650
    MSMVKSFVQW LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND 700
    LFFQPPPLTP TSKVYTIRPY FPKDEASVYK ICREMYDDGV GLPFQSQPDL 750
    IGDKLVGGLL SLSLDYCFVL EDEDGICGYA LGTVDVTPFI KKCKISWIPF 800
    MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP SLIKMDIHKK 850
    VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA 900
    KMEGFPKDVV ILGRSL 916
    Length:916
    Mass (Da):102,918
    Last modified:March 1, 2002 - v1
    Checksum:i4BA1746F0AF2E380
    GO
    Isoform 2 (identifier: Q8VIJ5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         250-398: Missing.

    Note: Lack hexosaminidase activity.

    Show »
    Length:767
    Mass (Da):86,010
    Checksum:i7DD1EF0F9D55E766
    GO
    Isoform 3 (identifier: Q8VIJ5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         250-345: Missing.

    Note: Lack hexosaminidase activity.

    Show »
    Length:820
    Mass (Da):91,993
    Checksum:i0A62BBF2AF31A0FD
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei250 – 398149Missing in isoform 2. CuratedVSP_020873Add
    BLAST
    Alternative sequencei250 – 34596Missing in isoform 3. CuratedVSP_020874Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY039679 mRNA. Translation: AAK72103.1.
    RefSeqiNP_571979.1. NM_131904.1. [Q8VIJ5-1]
    UniGeneiRn.162539.

    Genome annotation databases

    EnsembliENSRNOT00000065172; ENSRNOP00000059568; ENSRNOG00000017822. [Q8VIJ5-1]
    GeneIDi154968.
    KEGGirno:154968.
    UCSCiRGD:621077. rat. [Q8VIJ5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY039679 mRNA. Translation: AAK72103.1 .
    RefSeqi NP_571979.1. NM_131904.1. [Q8VIJ5-1 ]
    UniGenei Rn.162539.

    3D structure databases

    ProteinModelPortali Q8VIJ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000024198.

    Protein family/group databases

    CAZyi GH84. Glycoside Hydrolase Family 84.

    PTM databases

    PhosphoSitei Q8VIJ5.

    Proteomic databases

    PaxDbi Q8VIJ5.
    PRIDEi Q8VIJ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000065172 ; ENSRNOP00000059568 ; ENSRNOG00000017822 . [Q8VIJ5-1 ]
    GeneIDi 154968.
    KEGGi rno:154968.
    UCSCi RGD:621077. rat. [Q8VIJ5-1 ]

    Organism-specific databases

    CTDi 10724.
    RGDi 621077. Mgea5.

    Phylogenomic databases

    eggNOGi COG0454.
    GeneTreei ENSGT00390000007726.
    HOGENOMi HOG000044964.
    HOVERGENi HBG053044.
    InParanoidi Q8VIJ5.
    KOi K15719.
    OMAi LIKVDIH.
    OrthoDBi EOG7P02H7.
    PhylomeDBi Q8VIJ5.

    Miscellaneous databases

    NextBioi 620868.
    PROi Q8VIJ5.

    Gene expression databases

    Genevestigatori Q8VIJ5.

    Family and domain databases

    Gene3Di 3.40.630.30. 2 hits.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR011496. Beta-N-acetylglucosaminidase.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF07555. NAGidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF55729. SSF55729. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The O-GlcNAcase gene is a candidate for diabetes susceptibility in GK rats."
      Liu K., Paterson A.J., Van Tine B.A., Konrad R.J., Parlow A.F., Jimi S., Chin E. Jr., Kudlow J.E.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Fischer 344.
    2. "Characterization of the histone acetyltransferase (HAT) domain of a bifunctional protein with activable O-GlcNAcase and HAT activities."
      Toleman C., Paterson A.J., Whisenhunt T.R., Kudlow J.E.
      J. Biol. Chem. 279:53665-53673(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF ISOFORMS 2 AND 3, FUNCTION, MUTAGENESIS OF ASP-853; ASP-884 AND TYR-891.
      Strain: GK and Sprague-Dawley.
    3. "Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol."
      Dong D.-L., Hart G.W.
      J. Biol. Chem. 269:19321-19330(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY.
      Tissue: Spleen.

    Entry informationi

    Entry nameiNCOAT_RAT
    AccessioniPrimary (citable) accession number: Q8VIJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    External Data

    Dasty 3