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Protein

Nuclear receptor subfamily 2 group C member 1

Gene

Nr2c1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes including ESR1 and RARB. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Also activator of OCT4 gene expression. Plays a fundamental role in early embryogenesis and regulates embryonic stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi98 – 173Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri101 – 121NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri137 – 156NR C4-typePROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Receptor, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 2 group C member 1
Alternative name(s):
Orphan nuclear receptor TR2
Testicular receptor 2
Gene namesi
Name:Nr2c1
Synonyms:Tr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3200. Nr2c1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003694051 – 590Nuclear receptor subfamily 2 group C member 1Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei185PhosphoserineBy similarity1
Modified residuei203PhosphoserineBy similarity1
Modified residuei208PhosphothreonineBy similarity1
Modified residuei210Phosphothreonine; by MAPK1By similarity1
Cross-linki238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei568Phosphoserine; by PKCBy similarity1
Cross-linki575Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression (By similarity).By similarity
Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-210, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-568 by PKC is important for protein stability and function as activator of RARB (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8VIJ4.
PRIDEiQ8VIJ4.

PTM databases

PhosphoSitePlusiQ8VIJ4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006983.
GenevisibleiQ8VIJ4. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats (By similarity). Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity. Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding domain). Interacts with PIAS1; the interaction is required for sumoylation of NR2C1. Interacts with UBE2I; the interaction is required for sumoylation of NR2C1. Interacts with KAT2B; the interaction acts as a corepressor of gene expression (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009578.

Structurei

3D structure databases

ProteinModelPortaliQ8VIJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 166Required for interaction with KAT2BBy similarityAdd BLAST166
Regioni571 – 590Required for interaction with NRIP1By similarityAdd BLAST20

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri101 – 121NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri137 – 156NR C4-typePROSITE-ProRule annotationAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00760000118948.
HOGENOMiHOG000013058.
InParanoidiQ8VIJ4.
KOiK08543.
OMAiQEMQTNG.
OrthoDBiEOG091G099H.
PhylomeDBiQ8VIJ4.
TreeFamiTF316650.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiView protein in InterPro
IPR035500. NHR_like_domain.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
PfamiView protein in Pfam
PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiView protein in SMART
SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiView protein in PROSITE
PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VIJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIEEIAHQ IIDQQMGEIV TEQQTGQKIQ IVTALDHSTQ GKQFILANHE
60 70 80 90 100
GSTPGKVFLT TPDAAGVNQL FFASPDLSTP HLQLLTENSP DQGPNKVFDL
110 120 130 140 150
CVVCGDKASG RHYGAITCEG CKGFFKRSIR KNLVYSCRGS KDCIINKHHR
160 170 180 190 200
NRCQYCRLQR CIAFGMKQDS VQCERKPIEV SREKSSNCAA STEKIYIRKD
210 220 230 240 250
LRSPLAATPT FVTDSETARS TGLLDSGMFV NIHPSGIKTE PALLMTPDKA
260 270 280 290 300
ESCQGDLGTL ASVVTSLANL GKAKDLSHCG GDLPVVQSLR NGDTSFGAFH
310 320 330 340 350
QDIQTNGDVS RAFDNLAKAL TPGENPACQS PGESMEGSTH LIAGEPSCME
360 370 380 390 400
REGPLLSDSH VVFRLTMPSP MPEYLNVHYI GESASRLLFL SMHWALSIPS
410 420 430 440 450
FQALGQENSI SLVKAYWNEL FTLGLAQCWQ VMNVATILAT FVNCLHNSLQ
460 470 480 490 500
QDKMSPERRK LLMEHIFKLQ EFCNSMVKLC IDGHEYAYLK AIVLFSPDHP
510 520 530 540 550
GLENMELIEK FQEKAYVEFQ DYITRTYPDD TYRLSRLLLR LPALRLMNAT
560 570 580 590
ITEELFFKGL IGNVRIDSVI PHILKMEPAD YNSQIIGHSL
Length:590
Mass (Da):65,528
Last modified:March 1, 2002 - v1
Checksum:i000311C5FB6BA86F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26398 mRNA. Translation: AAL31316.1.
CH473960 Genomic DNA. Translation: EDM16889.1.
BC061822 mRNA. Translation: AAH61822.1.
RefSeqiNP_665723.1. NM_145780.1.
XP_008763438.1. XM_008765216.2.
XP_017450160.1. XM_017594671.1.
UniGeneiRn.79894.

Genome annotation databases

EnsembliENSRNOT00000009578; ENSRNOP00000009578; ENSRNOG00000006983.
ENSRNOT00000089228; ENSRNOP00000071901; ENSRNOG00000006983.
GeneIDi252924.
KEGGirno:252924.
UCSCiRGD:3200. rat.

Similar proteinsi

Entry informationi

Entry nameiNR2C1_RAT
AccessioniPrimary (citable) accession number: Q8VIJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families