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Protein

Mineralocorticoid receptor

Gene

Nr3c2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei764SteroidBy similarity1
Binding sitei770SteroidBy similarity1
Binding sitei811SteroidBy similarity1
Binding sitei939SteroidBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi603 – 666Nuclear receptorPROSITE-ProRule annotationAdd BLAST64
Zinc fingeri603 – 623NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri637 – 661NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • cellular sodium ion homeostasis Source: MGI
  • excretion Source: MGI
  • steroid hormone mediated signaling pathway Source: InterPro
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandLipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mineralocorticoid receptor
Short name:
MR
Alternative name(s):
Nuclear receptor subfamily 3 group C member 2
Gene namesi
Name:Nr3c2
Synonyms:Mlr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99459. Nr3c2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000536831 – 978Mineralocorticoid receptorAdd BLAST978

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250PhosphoserineCombined sources1
Modified residuei259PhosphoserineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VII8.
PaxDbiQ8VII8.
PRIDEiQ8VII8.

PTM databases

iPTMnetiQ8VII8.
PhosphoSitePlusiQ8VII8.

Expressioni

Tissue specificityi

Expressed in heart and kidney.1 Publication

Gene expression databases

CleanExiMM_NR3C2.

Interactioni

Subunit structurei

Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. Binds the coactivator NCOA2. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, TIF1 and NRIP1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105538.

Structurei

3D structure databases

ProteinModelPortaliQ8VII8.
SMRiQ8VII8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 602ModulatingAdd BLAST602
Regioni667 – 726HingeAdd BLAST60
Regioni727 – 978Steroid-bindingAdd BLAST252
Regioni776 – 779Important for coactivator bindingBy similarity4

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri603 – 623NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri637 – 661NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
HOGENOMiHOG000247011.
HOVERGENiHBG006336.
InParanoidiQ8VII8.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiView protein in InterPro
IPR035500. NHR_like_dom_sf.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
PfamiView protein in Pfam
PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
PRINTSiPR00047. STROIDFINGER.
SMARTiView protein in SMART
SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiView protein in PROSITE
PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VII8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METKGYHSLP EGLDMERRWS QVSQTLERSS LGPAERTNEN SYMEIVNVSC
60 70 80 90 100
VSGATPNNST QGSSKEKHEL LPCLQQDNSR SGILPSDIKT ELESKELSAT
110 120 130 140 150
VAESMGLYMD SVRDAEYTYD QQNQQGSLSP AKIYQNMEQL VKFYKENGHR
160 170 180 190 200
SSTLSAISRP LRSFMPDSGT SMNGGALRAI VKSPIICHEK SPSVCSPLNM
210 220 230 240 250
PSSVCSPAGI NSMSSSTASF GSFPVHSPIT QGTSLTCSPS VENRGSRSHS
260 270 280 290 300
PVHASNVGSP LSSPLSSMKS PISSPPSHCS VKSPVSSPNN VPLRSSVSSP
310 320 330 340 350
ANLNNSRCSV SSPSNTNNRS TLSSPTASTV GSIGSPISNA FSYTTSGASA
360 370 380 390 400
GAGAIQDMVP SPDTHEKGAH DVPFPKTEEV EKAISNGVTG QLNIVQYIKP
410 420 430 440 450
EPDGAFSSSC LGGNNKINPS SPFSVPIKQE SSKHSCSGAS FKGNPTVNPF
460 470 480 490 500
PFMDGSYFSF MDDKDYYSLS GILGPPVPGF DSSCEGSAFP GGIKQEPDDG
510 520 530 540 550
SYFPETSIPS SAIIGVNSGG QSFHYRIGAQ GTISLSRSPR DQSFQHLSSF
560 570 580 590 600
PPVNALVESW KPHGDLSSRR SDGYPVLEYI PENVSSSTLR SVSTGSSRPS
610 620 630 640 650
KICLVCGDEA SGCHYGVVTC GSCKVFFKRA VEHNYLCAGR NDCIIDKIRR
660 670 680 690 700
KNCPACRLQK CLQAGMNLGA RKSKKLGKLK GLHEEQPQQP PPPPPQSPEE
710 720 730 740 750
GTTYIAPTKE PSVNSALVPQ LASITRALTP SPSMILENIE PEIVYAGYDN
760 770 780 790 800
SKPDTAESLL STLNRLAGKQ MIQVVKWAKV LPGFKNLPLE DQITLIQYSW
810 820 830 840 850
MCLSSFALSW RSYKHTNSQF LYFAPDLVFN EEKMHQSAMY ELCQGMRQIS
860 870 880 890 900
LQFVRLQLTF EEYSIMKVLL LLSTVPKDGL KSQAAFEEMR TNYIKELRKM
910 920 930 940 950
VTKCPNSSGQ SWQRFYQLTK LLDSMHDLVN DLLEFCFYTF RESQALKVEF
960 970
PAMLVEIISD QLPKVESGNA KPLYFHRK
Length:978
Mass (Da):106,447
Last modified:April 30, 2003 - v2
Checksum:i4D6F2C870FB4050A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103E → Q in CAC86374 (PubMed:11997477).Curated1
Sequence conflicti106G → D in CAC86374 (PubMed:11997477).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC113941 Genomic DNA. No translation available.
AC116768 Genomic DNA. No translation available.
AC118019 Genomic DNA. No translation available.
AC119248 Genomic DNA. No translation available.
AJ311855 mRNA. Translation: CAC86374.1.
AJ311856 mRNA. Translation: CAC86375.1.
UniGeneiMm.324393.

Similar proteinsi

Entry informationi

Entry nameiMCR_MOUSE
AccessioniPrimary (citable) accession number: Q8VII8
Secondary accession number(s): Q8VII9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: November 22, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families