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Q8VII8

- MCR_MOUSE

UniProt

Q8VII8 - MCR_MOUSE

Protein

Mineralocorticoid receptor

Gene

Nr3c2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei764 – 7641SteroidBy similarity
    Binding sitei770 – 7701SteroidBy similarity
    Binding sitei811 – 8111SteroidBy similarity
    Binding sitei939 – 9391SteroidBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi603 – 66664Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri603 – 62321NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri637 – 66125NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. steroid binding Source: MGI
    4. steroid hormone receptor activity Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular sodium ion homeostasis Source: MGI
    2. excretion Source: MGI
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Lipid-binding, Metal-binding, Steroid-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mineralocorticoid receptor
    Short name:
    MR
    Alternative name(s):
    Nuclear receptor subfamily 3 group C member 2
    Gene namesi
    Name:Nr3c2
    Synonyms:Mlr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:99459. Nr3c2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus 1 PublicationPROSITE-ProRule annotation. Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
    Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane By similarity.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. receptor complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 978978Mineralocorticoid receptorPRO_0000053683Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8VII8.

    PTM databases

    PhosphoSiteiQ8VII8.

    Expressioni

    Tissue specificityi

    Expressed in heart and kidney.1 Publication

    Gene expression databases

    ArrayExpressiQ8VII8.
    BgeeiQ8VII8.
    CleanExiMM_NR3C2.
    GenevestigatoriQ8VII8.

    Interactioni

    Subunit structurei

    Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. Binds the coactivator NCOA2. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, TIF1 and NRIP1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VII8.
    SMRiQ8VII8. Positions 602-978.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 602602ModulatingAdd
    BLAST
    Regioni667 – 72660HingeAdd
    BLAST
    Regioni727 – 978252Steroid-bindingAdd
    BLAST
    Regioni776 – 7794Important for coactivator bindingBy similarity

    Domaini

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri603 – 62321NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri637 – 66125NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG294115.
    HOGENOMiHOG000247011.
    HOVERGENiHBG006336.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VII8-1 [UniParc]FASTAAdd to Basket

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    METKGYHSLP EGLDMERRWS QVSQTLERSS LGPAERTNEN SYMEIVNVSC    50
    VSGATPNNST QGSSKEKHEL LPCLQQDNSR SGILPSDIKT ELESKELSAT 100
    VAESMGLYMD SVRDAEYTYD QQNQQGSLSP AKIYQNMEQL VKFYKENGHR 150
    SSTLSAISRP LRSFMPDSGT SMNGGALRAI VKSPIICHEK SPSVCSPLNM 200
    PSSVCSPAGI NSMSSSTASF GSFPVHSPIT QGTSLTCSPS VENRGSRSHS 250
    PVHASNVGSP LSSPLSSMKS PISSPPSHCS VKSPVSSPNN VPLRSSVSSP 300
    ANLNNSRCSV SSPSNTNNRS TLSSPTASTV GSIGSPISNA FSYTTSGASA 350
    GAGAIQDMVP SPDTHEKGAH DVPFPKTEEV EKAISNGVTG QLNIVQYIKP 400
    EPDGAFSSSC LGGNNKINPS SPFSVPIKQE SSKHSCSGAS FKGNPTVNPF 450
    PFMDGSYFSF MDDKDYYSLS GILGPPVPGF DSSCEGSAFP GGIKQEPDDG 500
    SYFPETSIPS SAIIGVNSGG QSFHYRIGAQ GTISLSRSPR DQSFQHLSSF 550
    PPVNALVESW KPHGDLSSRR SDGYPVLEYI PENVSSSTLR SVSTGSSRPS 600
    KICLVCGDEA SGCHYGVVTC GSCKVFFKRA VEHNYLCAGR NDCIIDKIRR 650
    KNCPACRLQK CLQAGMNLGA RKSKKLGKLK GLHEEQPQQP PPPPPQSPEE 700
    GTTYIAPTKE PSVNSALVPQ LASITRALTP SPSMILENIE PEIVYAGYDN 750
    SKPDTAESLL STLNRLAGKQ MIQVVKWAKV LPGFKNLPLE DQITLIQYSW 800
    MCLSSFALSW RSYKHTNSQF LYFAPDLVFN EEKMHQSAMY ELCQGMRQIS 850
    LQFVRLQLTF EEYSIMKVLL LLSTVPKDGL KSQAAFEEMR TNYIKELRKM 900
    VTKCPNSSGQ SWQRFYQLTK LLDSMHDLVN DLLEFCFYTF RESQALKVEF 950
    PAMLVEIISD QLPKVESGNA KPLYFHRK 978
    Length:978
    Mass (Da):106,447
    Last modified:April 30, 2003 - v2
    Checksum:i4D6F2C870FB4050A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti103 – 1031E → Q in CAC86374. (PubMed:11997477)Curated
    Sequence conflicti106 – 1061G → D in CAC86374. (PubMed:11997477)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC113941 Genomic DNA. No translation available.
    AC116768 Genomic DNA. No translation available.
    AC118019 Genomic DNA. No translation available.
    AC119248 Genomic DNA. No translation available.
    AJ311855 mRNA. Translation: CAC86374.1.
    AJ311856 mRNA. Translation: CAC86375.1.
    UniGeneiMm.324393.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC113941 Genomic DNA. No translation available.
    AC116768 Genomic DNA. No translation available.
    AC118019 Genomic DNA. No translation available.
    AC119248 Genomic DNA. No translation available.
    AJ311855 mRNA. Translation: CAC86374.1 .
    AJ311856 mRNA. Translation: CAC86375.1 .
    UniGenei Mm.324393.

    3D structure databases

    ProteinModelPortali Q8VII8.
    SMRi Q8VII8. Positions 602-978.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    GuidetoPHARMACOLOGYi 626.

    PTM databases

    PhosphoSitei Q8VII8.

    Proteomic databases

    PRIDEi Q8VII8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:99459. Nr3c2.

    Phylogenomic databases

    eggNOGi NOG294115.
    HOGENOMi HOG000247011.
    HOVERGENi HBG006336.

    Miscellaneous databases

    ChiTaRSi NR3C2. mouse.
    PROi Q8VII8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VII8.
    Bgeei Q8VII8.
    CleanExi MM_NR3C2.
    Genevestigatori Q8VII8.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "Reversible cardiac fibrosis and heart failure induced by conditional expression of an antisense mRNA of the mineralocorticoid receptor in cardiomyocytes."
      Beggah A.T., Escoubet B., Puttini S., Cailmail S., Delage V., Ouvrard-Pascaud A., Bocchi B., Peuchmaur M., Delcayre C., Farman N., Jaisser F.
      Proc. Natl. Acad. Sci. U.S.A. 99:7160-7165(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-109 AND 182-288.
      Strain: C57BL/6 X DBA/2.
      Tissue: Heart.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease."
      Shibata S., Nagase M., Yoshida S., Kawarazaki W., Kurihara H., Tanaka H., Miyoshi J., Takai Y., Fujita T.
      Nat. Med. 14:1370-1376(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiMCR_MOUSE
    AccessioniPrimary (citable) accession number: Q8VII8
    Secondary accession number(s): Q8VII9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3