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Q8VII8

- MCR_MOUSE

UniProt

Q8VII8 - MCR_MOUSE

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Protein

Mineralocorticoid receptor

Gene

Nr3c2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei764 – 7641SteroidBy similarity
Binding sitei770 – 7701SteroidBy similarity
Binding sitei811 – 8111SteroidBy similarity
Binding sitei939 – 9391SteroidBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi603 – 66664Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri603 – 62321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri637 – 66125NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. steroid binding Source: MGI
  4. steroid hormone receptor activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular sodium ion homeostasis Source: MGI
  2. excretion Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Lipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mineralocorticoid receptor
Short name:
MR
Alternative name(s):
Nuclear receptor subfamily 3 group C member 2
Gene namesi
Name:Nr3c2
Synonyms:Mlr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:99459. Nr3c2.

Subcellular locationi

Cytoplasm By similarity. Nucleus 1 PublicationPROSITE-ProRule annotation. Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane (By similarity).By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
  4. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 978978Mineralocorticoid receptorPRO_0000053683Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei299 – 2991Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8VII8.

PTM databases

PhosphoSiteiQ8VII8.

Expressioni

Tissue specificityi

Expressed in heart and kidney.1 Publication

Gene expression databases

BgeeiQ8VII8.
CleanExiMM_NR3C2.
ExpressionAtlasiQ8VII8. baseline and differential.
GenevestigatoriQ8VII8.

Interactioni

Subunit structurei

Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. Binds the coactivator NCOA2. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, TIF1 and NRIP1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8VII8.
SMRiQ8VII8. Positions 602-978.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 602602ModulatingAdd
BLAST
Regioni667 – 72660HingeAdd
BLAST
Regioni727 – 978252Steroid-bindingAdd
BLAST
Regioni776 – 7794Important for coactivator bindingBy similarity

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri603 – 62321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri637 – 66125NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG294115.
HOGENOMiHOG000247011.
HOVERGENiHBG006336.
InParanoidiQ8VII8.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VII8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METKGYHSLP EGLDMERRWS QVSQTLERSS LGPAERTNEN SYMEIVNVSC
60 70 80 90 100
VSGATPNNST QGSSKEKHEL LPCLQQDNSR SGILPSDIKT ELESKELSAT
110 120 130 140 150
VAESMGLYMD SVRDAEYTYD QQNQQGSLSP AKIYQNMEQL VKFYKENGHR
160 170 180 190 200
SSTLSAISRP LRSFMPDSGT SMNGGALRAI VKSPIICHEK SPSVCSPLNM
210 220 230 240 250
PSSVCSPAGI NSMSSSTASF GSFPVHSPIT QGTSLTCSPS VENRGSRSHS
260 270 280 290 300
PVHASNVGSP LSSPLSSMKS PISSPPSHCS VKSPVSSPNN VPLRSSVSSP
310 320 330 340 350
ANLNNSRCSV SSPSNTNNRS TLSSPTASTV GSIGSPISNA FSYTTSGASA
360 370 380 390 400
GAGAIQDMVP SPDTHEKGAH DVPFPKTEEV EKAISNGVTG QLNIVQYIKP
410 420 430 440 450
EPDGAFSSSC LGGNNKINPS SPFSVPIKQE SSKHSCSGAS FKGNPTVNPF
460 470 480 490 500
PFMDGSYFSF MDDKDYYSLS GILGPPVPGF DSSCEGSAFP GGIKQEPDDG
510 520 530 540 550
SYFPETSIPS SAIIGVNSGG QSFHYRIGAQ GTISLSRSPR DQSFQHLSSF
560 570 580 590 600
PPVNALVESW KPHGDLSSRR SDGYPVLEYI PENVSSSTLR SVSTGSSRPS
610 620 630 640 650
KICLVCGDEA SGCHYGVVTC GSCKVFFKRA VEHNYLCAGR NDCIIDKIRR
660 670 680 690 700
KNCPACRLQK CLQAGMNLGA RKSKKLGKLK GLHEEQPQQP PPPPPQSPEE
710 720 730 740 750
GTTYIAPTKE PSVNSALVPQ LASITRALTP SPSMILENIE PEIVYAGYDN
760 770 780 790 800
SKPDTAESLL STLNRLAGKQ MIQVVKWAKV LPGFKNLPLE DQITLIQYSW
810 820 830 840 850
MCLSSFALSW RSYKHTNSQF LYFAPDLVFN EEKMHQSAMY ELCQGMRQIS
860 870 880 890 900
LQFVRLQLTF EEYSIMKVLL LLSTVPKDGL KSQAAFEEMR TNYIKELRKM
910 920 930 940 950
VTKCPNSSGQ SWQRFYQLTK LLDSMHDLVN DLLEFCFYTF RESQALKVEF
960 970
PAMLVEIISD QLPKVESGNA KPLYFHRK
Length:978
Mass (Da):106,447
Last modified:April 30, 2003 - v2
Checksum:i4D6F2C870FB4050A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031E → Q in CAC86374. (PubMed:11997477)Curated
Sequence conflicti106 – 1061G → D in CAC86374. (PubMed:11997477)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC113941 Genomic DNA. No translation available.
AC116768 Genomic DNA. No translation available.
AC118019 Genomic DNA. No translation available.
AC119248 Genomic DNA. No translation available.
AJ311855 mRNA. Translation: CAC86374.1.
AJ311856 mRNA. Translation: CAC86375.1.
UniGeneiMm.324393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC113941 Genomic DNA. No translation available.
AC116768 Genomic DNA. No translation available.
AC118019 Genomic DNA. No translation available.
AC119248 Genomic DNA. No translation available.
AJ311855 mRNA. Translation: CAC86374.1 .
AJ311856 mRNA. Translation: CAC86375.1 .
UniGenei Mm.324393.

3D structure databases

ProteinModelPortali Q8VII8.
SMRi Q8VII8. Positions 602-978.
ModBasei Search...
MobiDBi Search...

Chemistry

GuidetoPHARMACOLOGYi 626.

PTM databases

PhosphoSitei Q8VII8.

Proteomic databases

PRIDEi Q8VII8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:99459. Nr3c2.

Phylogenomic databases

eggNOGi NOG294115.
HOGENOMi HOG000247011.
HOVERGENi HBG006336.
InParanoidi Q8VII8.

Miscellaneous databases

ChiTaRSi NR3C2. mouse.
PROi Q8VII8.
SOURCEi Search...

Gene expression databases

Bgeei Q8VII8.
CleanExi MM_NR3C2.
ExpressionAtlasi Q8VII8. baseline and differential.
Genevestigatori Q8VII8.

Family and domain databases

Gene3Di 1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 1 hit.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Reversible cardiac fibrosis and heart failure induced by conditional expression of an antisense mRNA of the mineralocorticoid receptor in cardiomyocytes."
    Beggah A.T., Escoubet B., Puttini S., Cailmail S., Delage V., Ouvrard-Pascaud A., Bocchi B., Peuchmaur M., Delcayre C., Farman N., Jaisser F.
    Proc. Natl. Acad. Sci. U.S.A. 99:7160-7165(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-109 AND 182-288.
    Strain: C57BL/6 X DBA/2.
    Tissue: Heart.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease."
    Shibata S., Nagase M., Yoshida S., Kawarazaki W., Kurihara H., Tanaka H., Miyoshi J., Takai Y., Fujita T.
    Nat. Med. 14:1370-1376(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMCR_MOUSE
AccessioniPrimary (citable) accession number: Q8VII8
Secondary accession number(s): Q8VII9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: October 29, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3