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Q8VII8 (MCR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mineralocorticoid receptor

Short name=MR
Alternative name(s):
Nuclear receptor subfamily 3 group C member 2
Gene names
Name:Nr3c2
Synonyms:Mlr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length978 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. Ref.4

Subunit structure

Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. Binds the coactivator NCOA2. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, TIF1 and NRIP1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane By similarity. Ref.4

Tissue specificity

Expressed in heart and kidney. Ref.4

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Post-translational modification

Phosphorylated By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 978978Mineralocorticoid receptor
PRO_0000053683

Regions

DNA binding603 – 66664Nuclear receptor
Zinc finger603 – 62321NR C4-type
Zinc finger637 – 66125NR C4-type
Region1 – 602602Modulating
Region667 – 72660Hinge
Region727 – 978252Steroid-binding
Region776 – 7794Important for coactivator binding By similarity

Sites

Binding site7641Steroid By similarity
Binding site7701Steroid By similarity
Binding site8111Steroid By similarity
Binding site9391Steroid By similarity

Amino acid modifications

Modified residue2991Phosphoserine Ref.3

Experimental info

Sequence conflict1031E → Q in CAC86374. Ref.2
Sequence conflict1061G → D in CAC86374. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8VII8 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 4D6F2C870FB4050A

FASTA978106,447
        10         20         30         40         50         60 
METKGYHSLP EGLDMERRWS QVSQTLERSS LGPAERTNEN SYMEIVNVSC VSGATPNNST 

        70         80         90        100        110        120 
QGSSKEKHEL LPCLQQDNSR SGILPSDIKT ELESKELSAT VAESMGLYMD SVRDAEYTYD 

       130        140        150        160        170        180 
QQNQQGSLSP AKIYQNMEQL VKFYKENGHR SSTLSAISRP LRSFMPDSGT SMNGGALRAI 

       190        200        210        220        230        240 
VKSPIICHEK SPSVCSPLNM PSSVCSPAGI NSMSSSTASF GSFPVHSPIT QGTSLTCSPS 

       250        260        270        280        290        300 
VENRGSRSHS PVHASNVGSP LSSPLSSMKS PISSPPSHCS VKSPVSSPNN VPLRSSVSSP 

       310        320        330        340        350        360 
ANLNNSRCSV SSPSNTNNRS TLSSPTASTV GSIGSPISNA FSYTTSGASA GAGAIQDMVP 

       370        380        390        400        410        420 
SPDTHEKGAH DVPFPKTEEV EKAISNGVTG QLNIVQYIKP EPDGAFSSSC LGGNNKINPS 

       430        440        450        460        470        480 
SPFSVPIKQE SSKHSCSGAS FKGNPTVNPF PFMDGSYFSF MDDKDYYSLS GILGPPVPGF 

       490        500        510        520        530        540 
DSSCEGSAFP GGIKQEPDDG SYFPETSIPS SAIIGVNSGG QSFHYRIGAQ GTISLSRSPR 

       550        560        570        580        590        600 
DQSFQHLSSF PPVNALVESW KPHGDLSSRR SDGYPVLEYI PENVSSSTLR SVSTGSSRPS 

       610        620        630        640        650        660 
KICLVCGDEA SGCHYGVVTC GSCKVFFKRA VEHNYLCAGR NDCIIDKIRR KNCPACRLQK 

       670        680        690        700        710        720 
CLQAGMNLGA RKSKKLGKLK GLHEEQPQQP PPPPPQSPEE GTTYIAPTKE PSVNSALVPQ 

       730        740        750        760        770        780 
LASITRALTP SPSMILENIE PEIVYAGYDN SKPDTAESLL STLNRLAGKQ MIQVVKWAKV 

       790        800        810        820        830        840 
LPGFKNLPLE DQITLIQYSW MCLSSFALSW RSYKHTNSQF LYFAPDLVFN EEKMHQSAMY 

       850        860        870        880        890        900 
ELCQGMRQIS LQFVRLQLTF EEYSIMKVLL LLSTVPKDGL KSQAAFEEMR TNYIKELRKM 

       910        920        930        940        950        960 
VTKCPNSSGQ SWQRFYQLTK LLDSMHDLVN DLLEFCFYTF RESQALKVEF PAMLVEIISD 

       970 
QLPKVESGNA KPLYFHRK 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"Reversible cardiac fibrosis and heart failure induced by conditional expression of an antisense mRNA of the mineralocorticoid receptor in cardiomyocytes."
Beggah A.T., Escoubet B., Puttini S., Cailmail S., Delage V., Ouvrard-Pascaud A., Bocchi B., Peuchmaur M., Delcayre C., Farman N., Jaisser F.
Proc. Natl. Acad. Sci. U.S.A. 99:7160-7165(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-109 AND 182-288.
Strain: C57BL/6 X DBA/2.
Tissue: Heart.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease."
Shibata S., Nagase M., Yoshida S., Kawarazaki W., Kurihara H., Tanaka H., Miyoshi J., Takai Y., Fujita T.
Nat. Med. 14:1370-1376(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC113941 Genomic DNA. No translation available.
AC116768 Genomic DNA. No translation available.
AC118019 Genomic DNA. No translation available.
AC119248 Genomic DNA. No translation available.
AJ311855 mRNA. Translation: CAC86374.1.
AJ311856 mRNA. Translation: CAC86375.1.
UniGeneMm.324393.

3D structure databases

ProteinModelPortalQ8VII8.
SMRQ8VII8. Positions 602-978.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY626.

PTM databases

PhosphoSiteQ8VII8.

Proteomic databases

PRIDEQ8VII8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:99459. Nr3c2.

Phylogenomic databases

eggNOGNOG294115.
HOGENOMHOG000247011.
HOVERGENHBG006336.

Gene expression databases

ArrayExpressQ8VII8.
BgeeQ8VII8.
CleanExMM_NR3C2.
GenevestigatorQ8VII8.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNR3C2. mouse.
PROQ8VII8.
SOURCESearch...

Entry information

Entry nameMCR_MOUSE
AccessionPrimary (citable) accession number: Q8VII8
Secondary accession number(s): Q8VII9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: March 19, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot