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Q8VII8

- MCR_MOUSE

UniProt

Q8VII8 - MCR_MOUSE

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Protein
Mineralocorticoid receptor
Gene
Nr3c2, Mlr
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei764 – 7641Steroid By similarity
Binding sitei770 – 7701Steroid By similarity
Binding sitei811 – 8111Steroid By similarity
Binding sitei939 – 9391Steroid By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi603 – 66664Nuclear receptor
Add
BLAST
Zinc fingeri603 – 62321NR C4-type
Add
BLAST
Zinc fingeri637 – 66125NR C4-type
Add
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. steroid binding Source: MGI
  4. steroid hormone receptor activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular sodium ion homeostasis Source: MGI
  2. excretion Source: MGI
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Lipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mineralocorticoid receptor
Short name:
MR
Alternative name(s):
Nuclear receptor subfamily 3 group C member 2
Gene namesi
Name:Nr3c2
Synonyms:Mlr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:99459. Nr3c2.

Subcellular locationi

Cytoplasm By similarity. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity
Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane By similarity.1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 978978Mineralocorticoid receptor
PRO_0000053683Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei299 – 2991Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8VII8.

PTM databases

PhosphoSiteiQ8VII8.

Expressioni

Tissue specificityi

Expressed in heart and kidney.1 Publication

Gene expression databases

ArrayExpressiQ8VII8.
BgeeiQ8VII8.
CleanExiMM_NR3C2.
GenevestigatoriQ8VII8.

Interactioni

Subunit structurei

Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. Binds the coactivator NCOA2. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, TIF1 and NRIP1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8VII8.
SMRiQ8VII8. Positions 602-978.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 602602Modulating
Add
BLAST
Regioni667 – 72660Hinge
Add
BLAST
Regioni727 – 978252Steroid-binding
Add
BLAST
Regioni776 – 7794Important for coactivator binding By similarity

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG294115.
HOGENOMiHOG000247011.
HOVERGENiHBG006336.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VII8-1 [UniParc]FASTAAdd to Basket

« Hide

METKGYHSLP EGLDMERRWS QVSQTLERSS LGPAERTNEN SYMEIVNVSC    50
VSGATPNNST QGSSKEKHEL LPCLQQDNSR SGILPSDIKT ELESKELSAT 100
VAESMGLYMD SVRDAEYTYD QQNQQGSLSP AKIYQNMEQL VKFYKENGHR 150
SSTLSAISRP LRSFMPDSGT SMNGGALRAI VKSPIICHEK SPSVCSPLNM 200
PSSVCSPAGI NSMSSSTASF GSFPVHSPIT QGTSLTCSPS VENRGSRSHS 250
PVHASNVGSP LSSPLSSMKS PISSPPSHCS VKSPVSSPNN VPLRSSVSSP 300
ANLNNSRCSV SSPSNTNNRS TLSSPTASTV GSIGSPISNA FSYTTSGASA 350
GAGAIQDMVP SPDTHEKGAH DVPFPKTEEV EKAISNGVTG QLNIVQYIKP 400
EPDGAFSSSC LGGNNKINPS SPFSVPIKQE SSKHSCSGAS FKGNPTVNPF 450
PFMDGSYFSF MDDKDYYSLS GILGPPVPGF DSSCEGSAFP GGIKQEPDDG 500
SYFPETSIPS SAIIGVNSGG QSFHYRIGAQ GTISLSRSPR DQSFQHLSSF 550
PPVNALVESW KPHGDLSSRR SDGYPVLEYI PENVSSSTLR SVSTGSSRPS 600
KICLVCGDEA SGCHYGVVTC GSCKVFFKRA VEHNYLCAGR NDCIIDKIRR 650
KNCPACRLQK CLQAGMNLGA RKSKKLGKLK GLHEEQPQQP PPPPPQSPEE 700
GTTYIAPTKE PSVNSALVPQ LASITRALTP SPSMILENIE PEIVYAGYDN 750
SKPDTAESLL STLNRLAGKQ MIQVVKWAKV LPGFKNLPLE DQITLIQYSW 800
MCLSSFALSW RSYKHTNSQF LYFAPDLVFN EEKMHQSAMY ELCQGMRQIS 850
LQFVRLQLTF EEYSIMKVLL LLSTVPKDGL KSQAAFEEMR TNYIKELRKM 900
VTKCPNSSGQ SWQRFYQLTK LLDSMHDLVN DLLEFCFYTF RESQALKVEF 950
PAMLVEIISD QLPKVESGNA KPLYFHRK 978
Length:978
Mass (Da):106,447
Last modified:April 30, 2003 - v2
Checksum:i4D6F2C870FB4050A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031E → Q in CAC86374. 1 Publication
Sequence conflicti106 – 1061G → D in CAC86374. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC113941 Genomic DNA. No translation available.
AC116768 Genomic DNA. No translation available.
AC118019 Genomic DNA. No translation available.
AC119248 Genomic DNA. No translation available.
AJ311855 mRNA. Translation: CAC86374.1.
AJ311856 mRNA. Translation: CAC86375.1.
UniGeneiMm.324393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC113941 Genomic DNA. No translation available.
AC116768 Genomic DNA. No translation available.
AC118019 Genomic DNA. No translation available.
AC119248 Genomic DNA. No translation available.
AJ311855 mRNA. Translation: CAC86374.1 .
AJ311856 mRNA. Translation: CAC86375.1 .
UniGenei Mm.324393.

3D structure databases

ProteinModelPortali Q8VII8.
SMRi Q8VII8. Positions 602-978.
ModBasei Search...
MobiDBi Search...

Chemistry

GuidetoPHARMACOLOGYi 626.

PTM databases

PhosphoSitei Q8VII8.

Proteomic databases

PRIDEi Q8VII8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:99459. Nr3c2.

Phylogenomic databases

eggNOGi NOG294115.
HOGENOMi HOG000247011.
HOVERGENi HBG006336.

Miscellaneous databases

ChiTaRSi NR3C2. mouse.
PROi Q8VII8.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8VII8.
Bgeei Q8VII8.
CleanExi MM_NR3C2.
Genevestigatori Q8VII8.

Family and domain databases

Gene3Di 1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 1 hit.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Reversible cardiac fibrosis and heart failure induced by conditional expression of an antisense mRNA of the mineralocorticoid receptor in cardiomyocytes."
    Beggah A.T., Escoubet B., Puttini S., Cailmail S., Delage V., Ouvrard-Pascaud A., Bocchi B., Peuchmaur M., Delcayre C., Farman N., Jaisser F.
    Proc. Natl. Acad. Sci. U.S.A. 99:7160-7165(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-109 AND 182-288.
    Strain: C57BL/6 X DBA/2.
    Tissue: Heart.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease."
    Shibata S., Nagase M., Yoshida S., Kawarazaki W., Kurihara H., Tanaka H., Miyoshi J., Takai Y., Fujita T.
    Nat. Med. 14:1370-1376(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMCR_MOUSE
AccessioniPrimary (citable) accession number: Q8VII8
Secondary accession number(s): Q8VII9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: March 19, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi