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Protein

Choline transporter-like protein 1

Gene

Slc44a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable choline transporter. May be involved in membrane synthesis and myelin production.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Choline transporter-like protein 1
Alternative name(s):
Solute carrier family 44 member 1
CD_antigen: CD92
Gene namesi
Name:Slc44a1
Synonyms:Cd92, Cdw92, Ctl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621426. Slc44a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2928CytoplasmicSequence analysisAdd
BLAST
Transmembranei30 – 5021HelicalSequence analysisAdd
BLAST
Topological domaini51 – 211161ExtracellularSequence analysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence analysisAdd
BLAST
Topological domaini233 – 2375CytoplasmicSequence analysis
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Topological domaini259 – 28729ExtracellularSequence analysisAdd
BLAST
Transmembranei288 – 30821HelicalSequence analysisAdd
BLAST
Topological domaini309 – 3146CytoplasmicSequence analysis
Transmembranei315 – 33521HelicalSequence analysisAdd
BLAST
Topological domaini336 – 3372ExtracellularSequence analysis
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Topological domaini359 – 37921CytoplasmicSequence analysisAdd
BLAST
Transmembranei380 – 40021HelicalSequence analysisAdd
BLAST
Topological domaini401 – 44141ExtracellularSequence analysisAdd
BLAST
Transmembranei442 – 46221HelicalSequence analysisAdd
BLAST
Topological domaini463 – 53674CytoplasmicSequence analysisAdd
BLAST
Transmembranei537 – 55721HelicalSequence analysisAdd
BLAST
Topological domaini558 – 5658ExtracellularSequence analysis
Transmembranei566 – 58621HelicalSequence analysisAdd
BLAST
Topological domaini587 – 65670CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 656655Choline transporter-like protein 1PRO_0000191714Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei651 – 6511PhosphoserineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ8VII6.
PRIDEiQ8VII6.

PTM databases

SwissPalmiQ8VII6.
UniCarbKBiQ8VII6.

Expressioni

Tissue specificityi

Expressed in neurons, oligodendrocytes and astrocytes. Also expressed in the mucosal cell layer of the colon. In the developing brain, isoform 1 is expressed in both neurones and oligodendroglial cells, whereas isoform 2 is restricted to oligodendroglial cells.3 Publications

Inductioni

By leukemia inhibitory factor or retinoic acid in vitro. In vivo, induced during the axonal elongation period following axotomy.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024229.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi473 – 49119Cys-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1362. Eukaryota.
ENOG410XS0P. LUCA.
HOGENOMiHOG000231655.
HOVERGENiHBG080361.
InParanoidiQ8VII6.
KOiK06515.
PhylomeDBiQ8VII6.

Family and domain databases

InterProiIPR007603. Choline_transptr-like.
[Graphical view]
PANTHERiPTHR12385. PTHR12385. 1 hit.
PfamiPF04515. Choline_transpo. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VII6-1) [UniParc]FASTAAdd to basket

Also known as: CTL1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCCSSASAA QSSKREWKPL EDRSCTDIPW LLLFVLFCIG MGFICGFSVA
60 70 80 90 100
TGAAARLVSG YDSYGNICGQ RNAKLEAIAN SGLDHTHRKY VFFLDPCNLD
110 120 130 140 150
LINRKIKSMA LCVAACPRQE LKTLSDVQKF AEINGSALCS YNIKPSEYTL
160 170 180 190 200
TAKSSAFCPK LPVPASAPIP FFHRCAPVNI SCYAKFAEAL ITFVSDNSVL
210 220 230 240 250
HRLISGVMTS KEIILGLCLL SLVLSMILMV IIRYISRVLV WILTILVILG
260 270 280 290 300
SLGGTGVLWW LYAKQRRSPK ETVIPEQLQI AEDNLRALLI YAISATVFTV
310 320 330 340 350
ILFLIMLVMR KRVALTIALF HVAGKVFIHL PLLVFQPFWT FFALVLFWAY
360 370 380 390 400
WIMTLLFLGT TGSAVQNEQG FVEYKISGPL QYMWWYHVVG LIWISEFILA
410 420 430 440 450
CQQMTVAGAV VTYYFTRDKR NLPFTPILAS VNRLIRYHLG TVAKGSFIIT
460 470 480 490 500
LVKIPRMILM YIHSQLKGKE NACARCMLKS CICCLWCLEK CLSYLNQNAY
510 520 530 540 550
TATAINSTNF CTSAKDAFVI LVENALRVAA INTVGDFMLF LGKVLIVCST
560 570 580 590 600
GLAGIMLLNY QQDYTVWVLP LIIVCLFAFL VAHCFLSIYE MVVDVLFLCF
610 620 630 640 650
AIDTKYNDGS PGREFYMDKV LMEFVENSRK AMKEAGKGGA ADARKLKPMA

SGASSA
Length:656
Mass (Da):73,092
Last modified:March 1, 2002 - v1
Checksum:i217AAD02F6E4D138
GO
Isoform 2 (identifier: Q8VII6-2) [UniParc]FASTAAdd to basket

Also known as: CTL1b

The sequence of this isoform differs from the canonical sequence as follows:
     650-653: ASGA → LRKR
     654-656: Missing.

Show »
Length:653
Mass (Da):73,114
Checksum:i7B2DA56BC8782FF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti267 – 2671R → S in CAB75555 (PubMed:10677542).Curated
Sequence conflicti645 – 6451K → E in CAB75555 (PubMed:10677542).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei650 – 6534ASGA → LRKR in isoform 2. 1 PublicationVSP_015429
Alternative sequencei654 – 6563Missing in isoform 2. 1 PublicationVSP_015430

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245619 mRNA. Translation: CAB75555.1.
AJ420809 mRNA. Translation: CAD12728.1.
RefSeqiNP_001029024.1. NM_001033852.1.
NP_445944.2. NM_053492.3.
UniGeneiRn.97686.

Genome annotation databases

GeneIDi85254.
KEGGirno:85254.
UCSCiRGD:621426. rat. [Q8VII6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245619 mRNA. Translation: CAB75555.1.
AJ420809 mRNA. Translation: CAD12728.1.
RefSeqiNP_001029024.1. NM_001033852.1.
NP_445944.2. NM_053492.3.
UniGeneiRn.97686.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024229.

PTM databases

SwissPalmiQ8VII6.
UniCarbKBiQ8VII6.

Proteomic databases

PaxDbiQ8VII6.
PRIDEiQ8VII6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85254.
KEGGirno:85254.
UCSCiRGD:621426. rat. [Q8VII6-1]

Organism-specific databases

CTDi23446.
RGDi621426. Slc44a1.

Phylogenomic databases

eggNOGiKOG1362. Eukaryota.
ENOG410XS0P. LUCA.
HOGENOMiHOG000231655.
HOVERGENiHBG080361.
InParanoidiQ8VII6.
KOiK06515.
PhylomeDBiQ8VII6.

Miscellaneous databases

NextBioi617358.
PROiQ8VII6.

Family and domain databases

InterProiIPR007603. Choline_transptr-like.
[Graphical view]
PANTHERiPTHR12385. PTHR12385. 1 hit.
PfamiPF04515. Choline_transpo. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins."
    O'Regan S., Traiffort E., Ruat M., Cha N., Compaore D., Meunier F.-M.
    Proc. Natl. Acad. Sci. U.S.A. 97:1835-1840(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Molecular characterization of the family of choline transporter-like proteins and their splice variants."
    Traiffort E., Ruat M., O'Regan S., Meunier F.-M.
    J. Neurochem. 92:1116-1125(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  3. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-14; 57-71; 75-88; 90-104; 108-118; 161-174; 186-211; 271-286; 421-433; 516-527; 620-629; 631-644 AND 646-656, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Changes in mRNA for choline transporter-like protein following facial nerve transection."
    Che Y.H., Yamashita T., Higuchi H., Tohyama M.
    Brain Res. Mol. Brain Res. 101:122-125(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Molecular and functional characterization of an Na-independent choline transporter in rat astrocytes."
    Inazu M., Takeda H., Matsumiya T.
    J. Neurochem. 94:1427-1437(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Differential expression and regulation of the high-affinity choline transporter CHT1 and choline acetyltransferase in neurons of superior cervical ganglia."
    Lecomte M.-J., De Gois S., Guerci A., Ravassard P., Faucon Biguet N., Mallet J., Berrard S.
    Mol. Cell. Neurosci. 28:303-313(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCTL1_RAT
AccessioniPrimary (citable) accession number: Q8VII6
Secondary accession number(s): Q9JJZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2002
Last modified: February 17, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.