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Q8VIH1

- NOBOX_MOUSE

UniProt

Q8VIH1 - NOBOX_MOUSE

Protein

Homeobox protein NOBOX

Gene

Nobox

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Transcription factor which plays an essential role in postnatal follicle development. Binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'. Directly regulates the transcription of POU5F1 and GDF9 during early folliculogenesis.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi136 – 19560HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    4. sequence-specific DNA binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. oogenesis Source: UniProtKB-KW
    2. ovarian follicle development Source: MGI
    3. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Oogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein NOBOX
    Alternative name(s):
    Homeodomain-containing protein OG-2
    Newborn ovary homeobox protein
    Oocyte-specific homeobox protein
    Gene namesi
    Name:Nobox
    Synonyms:Og2x
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:108011. Nobox.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: UniProtKB-SubCell
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Male mice are fertile and show no obvious abnormality. Female mice lacking Nobox have normal gross anatomy and histology, but are infertile with atrophic ovaries that lack oocytes at 6 weeks of age.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi186 – 1861N → Q: Abolishes DNA-binding. 1 Publication
    Mutagenesisi187 – 1871R → H: Abolishes DNA-binding. 1 Publication
    Mutagenesisi192 – 1921R → Q: No effect on DNA-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 527527Homeobox protein NOBOXPRO_0000268866Add
    BLAST

    Proteomic databases

    PRIDEiQ8VIH1.

    PTM databases

    PhosphoSiteiQ8VIH1.

    Expressioni

    Tissue specificityi

    Specifically expressed in ovaries and testes. In ovaries, expressed in oocytes from primordial through antral follicles but not in granulosa cells, theca cells and corpora lutea.1 Publication

    Developmental stagei

    Expressed from E15.5.1 Publication

    Gene expression databases

    BgeeiQ8VIH1.
    CleanExiMM_NOBOX.
    GenevestigatoriQ8VIH1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VIH1.
    SMRiQ8VIH1. Positions 143-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi220 – 465246Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG325494.
    GeneTreeiENSGT00650000093445.
    HOGENOMiHOG000113810.
    HOVERGENiHBG082039.
    InParanoidiQ8VIH1.
    OMAiNSHEGDL.
    OrthoDBiEOG74J974.
    PhylomeDBiQ8VIH1.
    TreeFamiTF337576.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VIH1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPTEKLCKK MQGQEAGDKP RTAALETEGP LQDSALPIQD DQDKQSSLPR    50
    ASLGKRPLSK TSEELMDAGT CRVHKAPTAA ACGPQSEEEG CSPPERKAES 100
    LKPSISAVPG QATAGSLNSH EGDLKKESLE VTCQFRKKTR TLYRSDQLEE 150
    LERIFQEDHY PDSDKRHEIS QMVGVTPQRI MVWFQNRRAK WRKVEKLNEK 200
    ETKNGPAAPS ADSSQHRSAP ELLDPMPTDL EPGPVPPENI LDVFPEPPML 250
    LTSEQTLTPF QNNEGAERVA VTPPLLSPPP IRRANLPLPL GPVQTPQVLP 300
    PMRDVPGSDS IYKDKAYVSW GTSIASPPTY SNLEDLGSQD YQASSQLGSF 350
    QLSQAPHLPL FPSLQSQFPY LPPFPYPIPS SMPFLPPEDS LFSFPFGFSG 400
    DSSQDYCPGP PPGQILLQPP AENMGTGPWS GHCLPEPPFP RPHYPQALGQ 450
    PLGAEGYFPN LLPTPYALTM SKQSSLGLNG LLEGTRVETG SSLSKMSDEQ 500
    TSSSLEQPAL EEVRDKNKNS HAAGAKE 527
    Length:527
    Mass (Da):57,565
    Last modified:March 1, 2002 - v1
    Checksum:i1B6AF1323F785290
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti323 – 3264SIAS → RYGT in AAC52828. (PubMed:8855241)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY061761 mRNA. Translation: AAL29683.1.
    BC125280 mRNA. Translation: AAI25281.1.
    BC125282 mRNA. Translation: AAI25283.1.
    U65067 Genomic DNA. Translation: AAC52828.1.
    CCDSiCCDS20093.1.
    RefSeqiNP_570939.1. NM_130869.3.
    UniGeneiMm.17715.

    Genome annotation databases

    EnsembliENSMUST00000031749; ENSMUSP00000031749; ENSMUSG00000029736.
    GeneIDi18291.
    KEGGimmu:18291.
    UCSCiuc009bsp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY061761 mRNA. Translation: AAL29683.1 .
    BC125280 mRNA. Translation: AAI25281.1 .
    BC125282 mRNA. Translation: AAI25283.1 .
    U65067 Genomic DNA. Translation: AAC52828.1 .
    CCDSi CCDS20093.1.
    RefSeqi NP_570939.1. NM_130869.3.
    UniGenei Mm.17715.

    3D structure databases

    ProteinModelPortali Q8VIH1.
    SMRi Q8VIH1. Positions 143-192.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8VIH1.

    Proteomic databases

    PRIDEi Q8VIH1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031749 ; ENSMUSP00000031749 ; ENSMUSG00000029736 .
    GeneIDi 18291.
    KEGGi mmu:18291.
    UCSCi uc009bsp.1. mouse.

    Organism-specific databases

    CTDi 135935.
    MGIi MGI:108011. Nobox.

    Phylogenomic databases

    eggNOGi NOG325494.
    GeneTreei ENSGT00650000093445.
    HOGENOMi HOG000113810.
    HOVERGENi HBG082039.
    InParanoidi Q8VIH1.
    OMAi NSHEGDL.
    OrthoDBi EOG74J974.
    PhylomeDBi Q8VIH1.
    TreeFami TF337576.

    Miscellaneous databases

    NextBioi 293732.
    PROi Q8VIH1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VIH1.
    CleanExi MM_NOBOX.
    Genevestigatori Q8VIH1.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nobox is a homeobox-encoding gene preferentially expressed in primordial and growing oocytes."
      Suzumori N., Yan C., Matzuk M.M., Rajkovic A.
      Mech. Dev. 111:137-141(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: 129/SvEv.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Cloning and characterization of four murine homeobox genes."
      Rovescalli A.C., Asoh S., Nirenberg M.W.
      Proc. Natl. Acad. Sci. U.S.A. 93:10691-10696(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-326, DEVELOPMENTAL STAGE.
    4. "NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene expression."
      Rajkovic A., Pangas S.A., Ballow D., Suzumori N., Matzuk M.M.
      Science 305:1157-1159(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    5. "Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters."
      Choi Y., Rajkovic A.
      J. Biol. Chem. 281:35747-35756(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, MUTAGENESIS OF ASN-186.
    6. "NOBOX homeobox mutation causes premature ovarian failure."
      Qin Y., Choi Y., Zhao H., Simpson J.L., Chen Z.-J., Rajkovic A.
      Am. J. Hum. Genet. 81:576-581(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-187 AND ARG-192.

    Entry informationi

    Entry nameiNOBOX_MOUSE
    AccessioniPrimary (citable) accession number: Q8VIH1
    Secondary accession number(s): P70367
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3