Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein NOBOX

Gene

Nobox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which plays an essential role in postnatal follicle development. Binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'. Directly regulates the transcription of POU5F1 and GDF9 during early folliculogenesis.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi136 – 19560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  4. sequence-specific DNA binding Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. oogenesis Source: UniProtKB-KW
  2. ovarian follicle development Source: MGI
  3. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Oogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein NOBOX
Alternative name(s):
Homeodomain-containing protein OG-2
Newborn ovary homeobox protein
Oocyte-specific homeobox protein
Gene namesi
Name:Nobox
Synonyms:Og2x
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:108011. Nobox.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: UniProtKB-SubCell
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Male mice are fertile and show no obvious abnormality. Female mice lacking Nobox have normal gross anatomy and histology, but are infertile with atrophic ovaries that lack oocytes at 6 weeks of age.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi186 – 1861N → Q: Abolishes DNA-binding. 1 Publication
Mutagenesisi187 – 1871R → H: Abolishes DNA-binding. 1 Publication
Mutagenesisi192 – 1921R → Q: No effect on DNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Homeobox protein NOBOXPRO_0000268866Add
BLAST

Proteomic databases

PRIDEiQ8VIH1.

PTM databases

PhosphoSiteiQ8VIH1.

Expressioni

Tissue specificityi

Specifically expressed in ovaries and testes. In ovaries, expressed in oocytes from primordial through antral follicles but not in granulosa cells, theca cells and corpora lutea.1 Publication

Developmental stagei

Expressed from E15.5.1 Publication

Gene expression databases

BgeeiQ8VIH1.
CleanExiMM_NOBOX.
GenevestigatoriQ8VIH1.

Structurei

3D structure databases

ProteinModelPortaliQ8VIH1.
SMRiQ8VIH1. Positions 143-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi220 – 465246Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG325494.
GeneTreeiENSGT00650000093445.
HOGENOMiHOG000113810.
HOVERGENiHBG082039.
InParanoidiQ8VIH1.
OMAiGHCLPEP.
OrthoDBiEOG74J974.
PhylomeDBiQ8VIH1.
TreeFamiTF337576.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VIH1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPTEKLCKK MQGQEAGDKP RTAALETEGP LQDSALPIQD DQDKQSSLPR
60 70 80 90 100
ASLGKRPLSK TSEELMDAGT CRVHKAPTAA ACGPQSEEEG CSPPERKAES
110 120 130 140 150
LKPSISAVPG QATAGSLNSH EGDLKKESLE VTCQFRKKTR TLYRSDQLEE
160 170 180 190 200
LERIFQEDHY PDSDKRHEIS QMVGVTPQRI MVWFQNRRAK WRKVEKLNEK
210 220 230 240 250
ETKNGPAAPS ADSSQHRSAP ELLDPMPTDL EPGPVPPENI LDVFPEPPML
260 270 280 290 300
LTSEQTLTPF QNNEGAERVA VTPPLLSPPP IRRANLPLPL GPVQTPQVLP
310 320 330 340 350
PMRDVPGSDS IYKDKAYVSW GTSIASPPTY SNLEDLGSQD YQASSQLGSF
360 370 380 390 400
QLSQAPHLPL FPSLQSQFPY LPPFPYPIPS SMPFLPPEDS LFSFPFGFSG
410 420 430 440 450
DSSQDYCPGP PPGQILLQPP AENMGTGPWS GHCLPEPPFP RPHYPQALGQ
460 470 480 490 500
PLGAEGYFPN LLPTPYALTM SKQSSLGLNG LLEGTRVETG SSLSKMSDEQ
510 520
TSSSLEQPAL EEVRDKNKNS HAAGAKE
Length:527
Mass (Da):57,565
Last modified:March 1, 2002 - v1
Checksum:i1B6AF1323F785290
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3264SIAS → RYGT in AAC52828. (PubMed:8855241)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061761 mRNA. Translation: AAL29683.1.
BC125280 mRNA. Translation: AAI25281.1.
BC125282 mRNA. Translation: AAI25283.1.
U65067 Genomic DNA. Translation: AAC52828.1.
CCDSiCCDS20093.1.
RefSeqiNP_570939.1. NM_130869.3.
UniGeneiMm.17715.

Genome annotation databases

EnsembliENSMUST00000031749; ENSMUSP00000031749; ENSMUSG00000029736.
GeneIDi18291.
KEGGimmu:18291.
UCSCiuc009bsp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061761 mRNA. Translation: AAL29683.1.
BC125280 mRNA. Translation: AAI25281.1.
BC125282 mRNA. Translation: AAI25283.1.
U65067 Genomic DNA. Translation: AAC52828.1.
CCDSiCCDS20093.1.
RefSeqiNP_570939.1. NM_130869.3.
UniGeneiMm.17715.

3D structure databases

ProteinModelPortaliQ8VIH1.
SMRiQ8VIH1. Positions 143-192.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8VIH1.

Proteomic databases

PRIDEiQ8VIH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031749; ENSMUSP00000031749; ENSMUSG00000029736.
GeneIDi18291.
KEGGimmu:18291.
UCSCiuc009bsp.1. mouse.

Organism-specific databases

CTDi135935.
MGIiMGI:108011. Nobox.

Phylogenomic databases

eggNOGiNOG325494.
GeneTreeiENSGT00650000093445.
HOGENOMiHOG000113810.
HOVERGENiHBG082039.
InParanoidiQ8VIH1.
OMAiGHCLPEP.
OrthoDBiEOG74J974.
PhylomeDBiQ8VIH1.
TreeFamiTF337576.

Miscellaneous databases

NextBioi293732.
PROiQ8VIH1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VIH1.
CleanExiMM_NOBOX.
GenevestigatoriQ8VIH1.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nobox is a homeobox-encoding gene preferentially expressed in primordial and growing oocytes."
    Suzumori N., Yan C., Matzuk M.M., Rajkovic A.
    Mech. Dev. 111:137-141(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: 129/SvEv.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Cloning and characterization of four murine homeobox genes."
    Rovescalli A.C., Asoh S., Nirenberg M.W.
    Proc. Natl. Acad. Sci. U.S.A. 93:10691-10696(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-326, DEVELOPMENTAL STAGE.
  4. "NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene expression."
    Rajkovic A., Pangas S.A., Ballow D., Suzumori N., Matzuk M.M.
    Science 305:1157-1159(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters."
    Choi Y., Rajkovic A.
    J. Biol. Chem. 281:35747-35756(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, MUTAGENESIS OF ASN-186.
  6. "NOBOX homeobox mutation causes premature ovarian failure."
    Qin Y., Choi Y., Zhao H., Simpson J.L., Chen Z.-J., Rajkovic A.
    Am. J. Hum. Genet. 81:576-581(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-187 AND ARG-192.

Entry informationi

Entry nameiNOBOX_MOUSE
AccessioniPrimary (citable) accession number: Q8VIH1
Secondary accession number(s): P70367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.