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Q8VIH1 (NOBOX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein NOBOX
Alternative name(s):
Homeodomain-containing protein OG-2
Newborn ovary homeobox protein
Oocyte-specific homeobox protein
Gene names
Name:Nobox
Synonyms:Og2x
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor which plays an essential role in postnatal follicle development. Binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'. Directly regulates the transcription of POU5F1 and GDF9 during early folliculogenesis. Ref.4 Ref.5

Subcellular location

Nucleus.

Tissue specificity

Specifically expressed in ovaries and testes. In ovaries, expressed in oocytes from primordial through antral follicles but not in granulosa cells, theca cells and corpora lutea. Ref.1

Developmental stage

Expressed from E15.5. Ref.3

Disruption phenotype

Male mice are fertile and show no obvious abnormality. Female mice lacking Nobox have normal gross anatomy and histology, but are infertile with atrophic ovaries that lack oocytes at 6 weeks of age. Ref.4

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Homeobox protein NOBOX
PRO_0000268866

Regions

DNA binding136 – 19560Homeobox Ref.5
Compositional bias220 – 465246Pro-rich

Experimental info

Mutagenesis1861N → Q: Abolishes DNA-binding. Ref.5
Mutagenesis1871R → H: Abolishes DNA-binding. Ref.6
Mutagenesis1921R → Q: No effect on DNA-binding. Ref.6
Sequence conflict323 – 3264SIAS → RYGT in AAC52828. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8VIH1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 1B6AF1323F785290

FASTA52757,565
        10         20         30         40         50         60 
MEPTEKLCKK MQGQEAGDKP RTAALETEGP LQDSALPIQD DQDKQSSLPR ASLGKRPLSK 

        70         80         90        100        110        120 
TSEELMDAGT CRVHKAPTAA ACGPQSEEEG CSPPERKAES LKPSISAVPG QATAGSLNSH 

       130        140        150        160        170        180 
EGDLKKESLE VTCQFRKKTR TLYRSDQLEE LERIFQEDHY PDSDKRHEIS QMVGVTPQRI 

       190        200        210        220        230        240 
MVWFQNRRAK WRKVEKLNEK ETKNGPAAPS ADSSQHRSAP ELLDPMPTDL EPGPVPPENI 

       250        260        270        280        290        300 
LDVFPEPPML LTSEQTLTPF QNNEGAERVA VTPPLLSPPP IRRANLPLPL GPVQTPQVLP 

       310        320        330        340        350        360 
PMRDVPGSDS IYKDKAYVSW GTSIASPPTY SNLEDLGSQD YQASSQLGSF QLSQAPHLPL 

       370        380        390        400        410        420 
FPSLQSQFPY LPPFPYPIPS SMPFLPPEDS LFSFPFGFSG DSSQDYCPGP PPGQILLQPP 

       430        440        450        460        470        480 
AENMGTGPWS GHCLPEPPFP RPHYPQALGQ PLGAEGYFPN LLPTPYALTM SKQSSLGLNG 

       490        500        510        520 
LLEGTRVETG SSLSKMSDEQ TSSSLEQPAL EEVRDKNKNS HAAGAKE 

« Hide

References

« Hide 'large scale' references
[1]"Nobox is a homeobox-encoding gene preferentially expressed in primordial and growing oocytes."
Suzumori N., Yan C., Matzuk M.M., Rajkovic A.
Mech. Dev. 111:137-141(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: 129/SvEv.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Cloning and characterization of four murine homeobox genes."
Rovescalli A.C., Asoh S., Nirenberg M.W.
Proc. Natl. Acad. Sci. U.S.A. 93:10691-10696(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-326, DEVELOPMENTAL STAGE.
[4]"NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene expression."
Rajkovic A., Pangas S.A., Ballow D., Suzumori N., Matzuk M.M.
Science 305:1157-1159(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]"Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters."
Choi Y., Rajkovic A.
J. Biol. Chem. 281:35747-35756(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, MUTAGENESIS OF ASN-186.
[6]"NOBOX homeobox mutation causes premature ovarian failure."
Qin Y., Choi Y., Zhao H., Simpson J.L., Chen Z.-J., Rajkovic A.
Am. J. Hum. Genet. 81:576-581(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ARG-187 AND ARG-192.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY061761 mRNA. Translation: AAL29683.1.
BC125280 mRNA. Translation: AAI25281.1.
BC125282 mRNA. Translation: AAI25283.1.
U65067 Genomic DNA. Translation: AAC52828.1.
CCDSCCDS20093.1.
RefSeqNP_570939.1. NM_130869.3.
UniGeneMm.17715.

3D structure databases

ProteinModelPortalQ8VIH1.
SMRQ8VIH1. Positions 143-192.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8VIH1.

Proteomic databases

PRIDEQ8VIH1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031749; ENSMUSP00000031749; ENSMUSG00000029736.
GeneID18291.
KEGGmmu:18291.
UCSCuc009bsp.1. mouse.

Organism-specific databases

CTD135935.
MGIMGI:108011. Nobox.

Phylogenomic databases

eggNOGNOG325494.
GeneTreeENSGT00650000093445.
HOGENOMHOG000113810.
HOVERGENHBG082039.
InParanoidQ8VIH1.
OMANSHEGDL.
OrthoDBEOG74J974.
PhylomeDBQ8VIH1.
TreeFamTF337576.

Gene expression databases

BgeeQ8VIH1.
CleanExMM_NOBOX.
GenevestigatorQ8VIH1.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293732.
PROQ8VIH1.
SOURCESearch...

Entry information

Entry nameNOBOX_MOUSE
AccessionPrimary (citable) accession number: Q8VIH1
Secondary accession number(s): P70367
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot