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Protein

E3 ubiquitin-protein ligase TRAIP

Gene

Traip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase acting as a negative regulator of innate immune signaling. Inhibits activation of NF-kappa-B mediated by TNF. Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation (By similarity) (PubMed:17544371, PubMed:22945920). Involved in response to genotoxic lesions during genome replication. Promotes H2AX and RPA2 phosphorylation after replication-associated DNA damage and assists fork progression at UV-induced replication-blocking lesions during S phase. Might also play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity. The function in translesion synthesis is controversial (By similarity).By similarity2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 5044RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • receptor signaling protein activity Source: MGI
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • negative regulation of interferon-beta production Source: CACAO
  • negative regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
  • protein ubiquitination Source: FlyBase
  • signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRAIP (EC:6.3.2.-)
Alternative name(s):
TRAF-interacting protein
Gene namesi
Name:Traip
Synonyms:Trip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1096377. Traip.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmperinuclear region By similarity
  • Nucleus By similarity
  • Nucleusnucleolus By similarity

  • Note: In the nucleus, found in close proximity to PCNA, suggesting localization at replication foci. After UV irradiation, rapidly localizes to sites of DNA damage, where it colocalizes with phosphorylated histone H2AX (gamma-H2AX) and RPA2.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

TRAIP knockdown substantially increases LPS- and poly(I:C)-induced IFN-production in mouse peritoneal macrophages at both mRNA and protein levels.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71C → A: Fails to autoubiquitinate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470E3 ubiquitin-protein ligase TRAIPPRO_0000056192Add
BLAST

Post-translational modificationi

Autoubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8VIG6.
PRIDEiQ8VIG6.

PTM databases

iPTMnetiQ8VIG6.
PhosphoSiteiQ8VIG6.

Expressioni

Tissue specificityi

Detected in testis and thymus, and at lower levels in spleen.

Gene expression databases

BgeeiENSMUSG00000032586.
CleanExiMM_TRAIP.
ExpressionAtlasiQ8VIG6. baseline and differential.
GenevisibleiQ8VIG6. MM.

Interactioni

Subunit structurei

Interacts with POLK and POLN (By similarity). Binds TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 is part of the receptor-TRAF signaling complex. The C-terminus interacts with CYLD, however the interaction was not detected with the full-length protein (PubMed:17544371).By similarity2 Publications

Protein-protein interaction databases

BioGridi204309. 1 interaction.
STRINGi10090.ENSMUSP00000040001.

Structurei

3D structure databases

ProteinModelPortaliQ8VIG6.
SMRiQ8VIG6. Positions 5-57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni211 – 470260Interaction with CYLDBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili66 – 187122Sequence analysisAdd
BLAST
Coiled coili201 – 28080Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 5044RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0827. Eukaryota.
ENOG410YY6J. LUCA.
GeneTreeiENSGT00390000007696.
HOGENOMiHOG000054214.
HOVERGENiHBG059760.
InParanoidiQ8VIG6.
KOiK11985.
OMAiSADKEIM.
OrthoDBiEOG091G0QPY.
PhylomeDBiQ8VIG6.
TreeFamiTF317309.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR009053. Prefoldin.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VIG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPIRALCTIC SDFFDHSRDV AAIHCGHTFH LQCLIQWFET APSRTCPQCR
60 70 80 90 100
IQVGKKTIIN KLFFDLAQEE ENVLDAEFLK NELDSVKAQL SQKDREKRDS
110 120 130 140 150
QAIIDTLRDT LEERNATVES LQNALNKAEM LCSTLKKQMK FLEQRQDETK
160 170 180 190 200
QAREEAHRLK CKMKTMEQIE LLLQSQRSEV EEMIRDMGVG QSAVEQLAVY
210 220 230 240 250
CVSLKKEYEN LKEARKATGE LADRLKKDLV SSRSKLKTLN TELDQAKLEL
260 270 280 290 300
RSAQKDLQSA DQEITSLRKK LMILQGTLSL PPATNETVSR LVFESPAPVE
310 320 330 340 350
MMNPRLHQPP FGDEIDLNTT FDVNTPPTQT SGSQHCLPKK LCLERARSPM
360 370 380 390 400
QNVLKKVHKV SKPESQLSLG GQRCVGELDE ELAGAFPLFI RNAVLGQKQP
410 420 430 440 450
NRTTAESRCS TDVVRIGFDG LGGRTKFIQP RDTTIIRPVP VKSKAKSKQK
460 470
VRIKTVSSAS QPKLDTFLCQ
Length:470
Mass (Da):53,149
Last modified:May 9, 2003 - v2
Checksum:iEBFABC49A9F4BF2E
GO
Isoform 2 (identifier: Q8VIG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     207-223: EYENLKEARKATGELAD → CVSSGVPLPSSFVMQIL
     224-470: Missing.

Note: No experimental confirmation available.
Show »
Length:223
Mass (Da):25,585
Checksum:i412291C205C43071
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 52RA → LS in AAB52994 (PubMed:9104814).Curated
Sequence conflicti265 – 2651T → M in AAH17374 (PubMed:15489334).Curated
Sequence conflicti283 – 2831A → R in AAB52994 (PubMed:9104814).Curated
Sequence conflicti409 – 4091C → S in AAB52994 (PubMed:9104814).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei207 – 22317EYENL…GELAD → CVSSGVPLPSSFVMQIL in isoform 2. 1 PublicationVSP_007406Add
BLAST
Alternative sequencei224 – 470247Missing in isoform 2. 1 PublicationVSP_007407Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77844 mRNA. Translation: AAB52994.1.
AK012786 mRNA. Translation: BAB28469.1.
AK012948 mRNA. Translation: BAB28567.1.
BC006929 mRNA. Translation: AAH06929.1.
BC017374 mRNA. Translation: AAH17374.1.
CCDSiCCDS23511.1. [Q8VIG6-1]
RefSeqiNP_035764.2. NM_011634.3. [Q8VIG6-1]
XP_011241178.1. XM_011242876.1. [Q8VIG6-1]
UniGeneiMm.297870.

Genome annotation databases

EnsembliENSMUST00000049348; ENSMUSP00000040001; ENSMUSG00000032586. [Q8VIG6-1]
GeneIDi22036.
KEGGimmu:22036.
UCSCiuc009rnn.1. mouse. [Q8VIG6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77844 mRNA. Translation: AAB52994.1.
AK012786 mRNA. Translation: BAB28469.1.
AK012948 mRNA. Translation: BAB28567.1.
BC006929 mRNA. Translation: AAH06929.1.
BC017374 mRNA. Translation: AAH17374.1.
CCDSiCCDS23511.1. [Q8VIG6-1]
RefSeqiNP_035764.2. NM_011634.3. [Q8VIG6-1]
XP_011241178.1. XM_011242876.1. [Q8VIG6-1]
UniGeneiMm.297870.

3D structure databases

ProteinModelPortaliQ8VIG6.
SMRiQ8VIG6. Positions 5-57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204309. 1 interaction.
STRINGi10090.ENSMUSP00000040001.

PTM databases

iPTMnetiQ8VIG6.
PhosphoSiteiQ8VIG6.

Proteomic databases

PaxDbiQ8VIG6.
PRIDEiQ8VIG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049348; ENSMUSP00000040001; ENSMUSG00000032586. [Q8VIG6-1]
GeneIDi22036.
KEGGimmu:22036.
UCSCiuc009rnn.1. mouse. [Q8VIG6-1]

Organism-specific databases

CTDi10293.
MGIiMGI:1096377. Traip.

Phylogenomic databases

eggNOGiKOG0827. Eukaryota.
ENOG410YY6J. LUCA.
GeneTreeiENSGT00390000007696.
HOGENOMiHOG000054214.
HOVERGENiHBG059760.
InParanoidiQ8VIG6.
KOiK11985.
OMAiSADKEIM.
OrthoDBiEOG091G0QPY.
PhylomeDBiQ8VIG6.
TreeFamiTF317309.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiTraip. mouse.
PROiQ8VIG6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032586.
CleanExiMM_TRAIP.
ExpressionAtlasiQ8VIG6. baseline and differential.
GenevisibleiQ8VIG6. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR009053. Prefoldin.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF46579. SSF46579. 1 hit.
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRAIP_MOUSE
AccessioniPrimary (citable) accession number: Q8VIG6
Secondary accession number(s): O08854, Q922M8, Q9CPP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 9, 2003
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.