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Q8VIG1 (REST_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RE1-silencing transcription factor
Alternative name(s):
Neural-restrictive silencer factor
Gene names
Name:Rest
Synonyms:Nrsf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1082 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression By similarity.

Subunit structure

Interacts with SIN3A, SIN3B and RCOR1 By similarity. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in many non-neuronal tissues including the heart and liver. Ref.6

Developmental stage

During embryogenesis, expressed at high levels in non-neuronal and differentiated peripheral nervous tissues, but is not expressed in differentiating neurons in the CNS. Ref.5 Ref.6

Sequence similarities

Contains 9 C2H2-type zinc fingers.

Sequence caution

The sequence AAH96434.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcardiac muscle cell myoblast differentiation

Inferred from direct assay PubMed 21199191. Source: UniProtKB

cellular response to drug

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to electrical stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to glucocorticoid stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

hematopoietic progenitor cell differentiation

Inferred from mutant phenotype PubMed 24029230. Source: MGI

histone H4 deacetylation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation by host of viral transcription

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of aldosterone biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of amniotic stem cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of calcium ion-dependent exocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cortisol biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of dense core granule biogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of insulin secretion

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of neurogenesis

Inferred from genetic interaction PubMed 17403776. Source: MGI

negative regulation of neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 17130167. Source: UniProtKB

positive regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

potassium ion transmembrane transport

Inferred from sequence or structural similarity. Source: GOC

   Cellular_componentcytosol

Inferred from direct assay PubMed 21199191. Source: UniProtKB

nucleus

Inferred from direct assay PubMed 14645515PubMed 21199191PubMed 14645515. Source: UniProtKB

transcriptional repressor complex

Inferred from direct assay PubMed 17130167. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15240883. Source: MGI

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 17130167. Source: UniProtKB

chromatin binding

Inferred from direct assay PubMed 16684532. Source: MGI

core promoter binding

Inferred from sequence or structural similarity. Source: UniProtKB

core promoter sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

outward rectifier potassium channel activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 14645515PubMed 17130167PubMed 14645515. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VIG1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VIG1-2)

Also known as: N16; REST4;

The sequence of this isoform differs from the canonical sequence as follows:
     323-328: GEKPFK → ECDLAG
     329-1082: Missing.
Isoform 3 (identifier: Q8VIG1-3)

Also known as: N28;

The sequence of this isoform differs from the canonical sequence as follows:
     324-332: EKPFKCDQC → CDLVGYVFR
     333-1082: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10821082RE1-silencing transcription factor
PRO_0000269548

Regions

Zinc finger154 – 17623C2H2-type 1
Zinc finger211 – 23525C2H2-type 2
Zinc finger243 – 26523C2H2-type 3
Zinc finger271 – 29323C2H2-type 4
Zinc finger299 – 32123C2H2-type 5
Zinc finger327 – 35024C2H2-type 6
Zinc finger356 – 37823C2H2-type 7
Zinc finger384 – 40724C2H2-type 8
Zinc finger1036 – 105823C2H2-type 9
Region32 – 11786Interaction with SIN3A By similarity
Region43 – 5715Interaction with SIN3B By similarity
Region985 – 106379Interaction with RCOR1 By similarity
Compositional bias395 – 602208Lys-rich
Compositional bias661 – 792132Pro-rich

Natural variations

Alternative sequence323 – 3286GEKPFK → ECDLAG in isoform 2.
VSP_022069
Alternative sequence324 – 3329EKPFKCDQC → CDLVGYVFR in isoform 3.
VSP_022070
Alternative sequence329 – 1082754Missing in isoform 2.
VSP_022071
Alternative sequence333 – 1082750Missing in isoform 3.
VSP_022072

Experimental info

Sequence conflict4141T → R in BAB82460. Ref.1
Sequence conflict4851T → S in BAB82460. Ref.1
Sequence conflict5561G → S in BAB82460. Ref.1
Sequence conflict5841K → N in BAB82460. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 0D3616BC0820BC2F

FASTA1,082117,784
        10         20         30         40         50         60 
MATQVMGQSS GGGSLFNNSA NMGMALTNDM YDLHELSKAE LAAPQLIMLA NVALTGEASG 

        70         80         90        100        110        120 
SCCDYLVGEE RQMAELMPVG DNHFSESEGE GLEESADLKG LENMELGSLE LSAVEPQPVF 

       130        140        150        160        170        180 
EASAAPEIYS ANKDPAPETP VAEDKCRSSK AKPFRCKPCQ YEAESEEQFV HHIRIHSAKK 

       190        200        210        220        230        240 
FFVEESAEKQ AKAWESGSSP AEEGEFSKGP IRCDRCGYNT NRYDHYMAHL KHHLRAGENE 

       250        260        270        280        290        300 
RIYKCIICTY TTVSEYHWRK HLRNHFPRKV YTCSKCNYFS DRKNNYVQHV RTHTGERPYK 

       310        320        330        340        350        360 
CELCPYSSSQ KTHLTRHMRT HSGEKPFKCD QCNYVASNQH EVTRHARQVH NGPKPLNCPH 

       370        380        390        400        410        420 
CDYKTADRSN FKKHVELHVN PRQFNCPVCD YAASKKCNLQ YHFKSKHPTC PSKTMDVSKV 

       430        440        450        460        470        480 
KLKKTKKREA DLLNNAVSNE KMENEQTKTK GDVSGKKNEK PVKAVGKDAS KEKKPGSSVS 

       490        500        510        520        530        540 
VVQVTTRTRK SAVAAETKAA EVKHTDGQTG NNPEKPCKAK KNKRKKDAEA HPSEEPVNEG 

       550        560        570        580        590        600 
PVTKKKKKSE CKSKIGTNVP KGGGRAEERP GVKKQSASLK KGTKKTPPKT KTSKKGGKLA 

       610        620        630        640        650        660 
PKGMGQTEPS SGALAQVGVS PDPALIQAEV TGSGSSQTEL PSPMDIAKSE PAQMEVSLTG 

       670        680        690        700        710        720 
PPPVEPAQME PSPAKPPQVE APTYPQPPQR GPAPPTGPAP PTGPAPPTEP APPTGLAEME 

       730        740        750        760        770        780 
PSPTEPSQKE PPPSMEPPCP EELPQAEPPP MEDCQKELPS PVEPAQIEVA QTAPTQVQEE 

       790        800        810        820        830        840 
PPPVSEPPRV KPTKRSSLRK DRAEKELSLL SEMARQEQVL MGVGLVPVRD SKLLKGNKSA 

       850        860        870        880        890        900 
QDPPAPPSPS PKGNSREETP KDQEMVSDGE GTIVFPLKKG GPEEAGESPA ELAALKESAR 

       910        920        930        940        950        960 
VSSSEQNSAM PEGGASHSKC QTGSSGLCDV DTEQKTDTVP MKDSAAEPVS PPTPTVDRDA 

       970        980        990       1000       1010       1020 
GSPAVVASPP ITLAENESQE IDEDEGIHSH DGSDLSDNMS EGSDDSGLHG ARPTPPEATS 

      1030       1040       1050       1060       1070       1080 
KNGKAGLAGK VTEGEFVCIF CDRSFRKEKD YSKHLNRHLV NVYFLEEAAE EQEEQEEREE 


QE 

« Hide

Isoform 2 (N16) (REST4) [UniParc].

Checksum: F47E141348260843
Show »

FASTA32836,953
Isoform 3 (N28) [UniParc].

Checksum: 15B8F53514610413
Show »

FASTA33237,475

References

« Hide 'large scale' references
[1]"Alternative promoter and splicing generate multiple isoforms of the neural-restrictive silencer factor NRSF/REST,a general transcriptional repressor for multiple neuron specific genes."
Kojima T., Naruse Y., Mori N.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: 129.
[2]"Cloning of the REST4 splice variant of the mouse REST protein."
Puhl H.L. III, Ikeda S.R.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Neuroblastoma.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N-3.
Tissue: Mammary tumor.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-853 (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Liver and Spleen.
[5]"REST: a mammalian silencer protein that restricts sodium channel gene expression to neurons."
Chong J.A., Tapia-Ramirez J., Kim S., Toledo-Aral J.J., Zheng Y., Boutros M.C., Altshuller Y.M., Frohman M.A., Kraner S.D., Mandel G.
Cell 80:949-957(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes."
Schoenherr C.J., Anderson D.J.
Science 267:1360-1363(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]"Neuron-specific splicing of zinc finger transcription factor REST/NRSF/XBR is frequent in neuroblastomas and conserved in human, mouse and rat."
Palm K., Metsis M., Timmusk T.
Brain Res. Mol. Brain Res. 72:30-39(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS 2 AND 3).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB024496 mRNA. Translation: BAB82460.1.
DQ644039 mRNA. Translation: ABG35129.1.
BC127065 mRNA. Translation: AAI27066.1.
BC096434 mRNA. Translation: AAH96434.1. Sequence problems.
AK004945 mRNA. Translation: BAB23689.3.
AK156514 mRNA. Translation: BAE33741.1.
CCDSCCDS19372.1. [Q8VIG1-1]
RefSeqNP_035393.2. NM_011263.2. [Q8VIG1-1]
XP_006534904.1. XM_006534841.1. [Q8VIG1-1]
XP_006534905.1. XM_006534842.1. [Q8VIG1-1]
UniGeneMm.28840.
Mm.482359.

3D structure databases

ProteinModelPortalQ8VIG1.
SMRQ8VIG1. Positions 147-405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202864. 6 interactions.
IntActQ8VIG1. 1 interaction.
MINTMINT-270819.

PTM databases

PhosphoSiteQ8VIG1.

Proteomic databases

PRIDEQ8VIG1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000080359; ENSMUSP00000079231; ENSMUSG00000029249. [Q8VIG1-1]
ENSMUST00000113449; ENSMUSP00000109076; ENSMUSG00000029249. [Q8VIG1-1]
GeneID19712.
KEGGmmu:19712.
UCSCuc008xvz.2. mouse. [Q8VIG1-1]

Organism-specific databases

CTD5978.
MGIMGI:104897. Rest.

Phylogenomic databases

eggNOGNOG271556.
GeneTreeENSGT00530000063458.
HOGENOMHOG000234631.
HOVERGENHBG093893.
InParanoidA0JNY8.
KOK09222.
OMAVQKEPVQ.
OrthoDBEOG73FQM4.
TreeFamTF332861.

Gene expression databases

BgeeQ8VIG1.
CleanExMM_REST.
GenevestigatorQ8VIG1.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR027775. C2H2_Znf_fam.
IPR027757. REST.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERPTHR10032. PTHR10032. 1 hit.
PTHR10032:SF71. PTHR10032:SF71. 1 hit.
SMARTSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio297094.
PROQ8VIG1.
SOURCESearch...

Entry information

Entry nameREST_MOUSE
AccessionPrimary (citable) accession number: Q8VIG1
Secondary accession number(s): A0JNY8 expand/collapse secondary AC list , Q155C6, Q3U0W0, Q4VAD6, Q9CW43
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot