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Q8VIG1

- REST_MOUSE

UniProt

Q8VIG1 - REST_MOUSE

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Protein
RE1-silencing transcription factor
Gene
Rest, Nrsf
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri154 – 17623C2H2-type 1
Add
BLAST
Zinc fingeri211 – 23525C2H2-type 2
Add
BLAST
Zinc fingeri243 – 26523C2H2-type 3
Add
BLAST
Zinc fingeri271 – 29323C2H2-type 4
Add
BLAST
Zinc fingeri299 – 32123C2H2-type 5
Add
BLAST
Zinc fingeri327 – 35024C2H2-type 6
Add
BLAST
Zinc fingeri356 – 37823C2H2-type 7
Add
BLAST
Zinc fingeri384 – 40724C2H2-type 8
Add
BLAST
Zinc fingeri1036 – 105823C2H2-type 9
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
  3. chromatin binding Source: MGI
  4. core promoter binding Source: UniProtKB
  5. core promoter sequence-specific DNA binding Source: Ensembl
  6. metal ion binding Source: UniProtKB-KW
  7. outward rectifier potassium channel activity Source: UniProtKB
  8. protein binding Source: UniProtKB
  9. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  10. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. cardiac muscle cell myoblast differentiation Source: UniProtKB
  2. cellular response to drug Source: UniProtKB
  3. cellular response to electrical stimulus Source: UniProtKB
  4. cellular response to glucocorticoid stimulus Source: UniProtKB
  5. hematopoietic progenitor cell differentiation Source: MGI
  6. histone H4 deacetylation Source: UniProtKB
  7. negative regulation by host of viral transcription Source: UniProtKB
  8. negative regulation of aldosterone biosynthetic process Source: UniProtKB
  9. negative regulation of amniotic stem cell differentiation Source: UniProtKB
  10. negative regulation of calcium ion-dependent exocytosis Source: UniProtKB
  11. negative regulation of cell proliferation Source: UniProtKB
  12. negative regulation of cortisol biosynthetic process Source: UniProtKB
  13. negative regulation of dense core granule biogenesis Source: UniProtKB
  14. negative regulation of gene expression Source: UniProtKB
  15. negative regulation of insulin secretion Source: UniProtKB
  16. negative regulation of neurogenesis Source: MGI
  17. negative regulation of neuron differentiation Source: UniProtKB
  18. negative regulation of transcription, DNA-templated Source: UniProtKB
  19. positive regulation of apoptotic process Source: UniProtKB
  20. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  21. positive regulation of transcription, DNA-templated Source: Ensembl
  22. potassium ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RE1-silencing transcription factor
Alternative name(s):
Neural-restrictive silencer factor
Gene namesi
Name:Rest
Synonyms:Nrsf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:104897. Rest.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10821082RE1-silencing transcription factor
PRO_0000269548Add
BLAST

Proteomic databases

PRIDEiQ8VIG1.

PTM databases

PhosphoSiteiQ8VIG1.

Expressioni

Tissue specificityi

Expressed in many non-neuronal tissues including the heart and liver.1 Publication

Developmental stagei

During embryogenesis, expressed at high levels in non-neuronal and differentiated peripheral nervous tissues, but is not expressed in differentiating neurons in the CNS.2 Publications

Gene expression databases

BgeeiQ8VIG1.
CleanExiMM_REST.
GenevestigatoriQ8VIG1.

Interactioni

Subunit structurei

Interacts with SIN3A, SIN3B and RCOR1 By similarity. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 By similarity.

Protein-protein interaction databases

BioGridi202864. 6 interactions.
IntActiQ8VIG1. 1 interaction.
MINTiMINT-270819.

Structurei

3D structure databases

ProteinModelPortaliQ8VIG1.
SMRiQ8VIG1. Positions 147-405.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni32 – 11786Interaction with SIN3A By similarity
Add
BLAST
Regioni43 – 5715Interaction with SIN3B By similarity
Add
BLAST
Regioni985 – 106379Interaction with RCOR1 By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi395 – 602208Lys-rich
Add
BLAST
Compositional biasi661 – 792132Pro-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG271556.
GeneTreeiENSGT00530000063458.
HOGENOMiHOG000234631.
HOVERGENiHBG093893.
InParanoidiA0JNY8.
KOiK09222.
OMAiVQKEPVQ.
OrthoDBiEOG73FQM4.
TreeFamiTF332861.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR027757. REST.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
PTHR10032:SF71. PTHR10032:SF71. 1 hit.
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VIG1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATQVMGQSS GGGSLFNNSA NMGMALTNDM YDLHELSKAE LAAPQLIMLA     50
NVALTGEASG SCCDYLVGEE RQMAELMPVG DNHFSESEGE GLEESADLKG 100
LENMELGSLE LSAVEPQPVF EASAAPEIYS ANKDPAPETP VAEDKCRSSK 150
AKPFRCKPCQ YEAESEEQFV HHIRIHSAKK FFVEESAEKQ AKAWESGSSP 200
AEEGEFSKGP IRCDRCGYNT NRYDHYMAHL KHHLRAGENE RIYKCIICTY 250
TTVSEYHWRK HLRNHFPRKV YTCSKCNYFS DRKNNYVQHV RTHTGERPYK 300
CELCPYSSSQ KTHLTRHMRT HSGEKPFKCD QCNYVASNQH EVTRHARQVH 350
NGPKPLNCPH CDYKTADRSN FKKHVELHVN PRQFNCPVCD YAASKKCNLQ 400
YHFKSKHPTC PSKTMDVSKV KLKKTKKREA DLLNNAVSNE KMENEQTKTK 450
GDVSGKKNEK PVKAVGKDAS KEKKPGSSVS VVQVTTRTRK SAVAAETKAA 500
EVKHTDGQTG NNPEKPCKAK KNKRKKDAEA HPSEEPVNEG PVTKKKKKSE 550
CKSKIGTNVP KGGGRAEERP GVKKQSASLK KGTKKTPPKT KTSKKGGKLA 600
PKGMGQTEPS SGALAQVGVS PDPALIQAEV TGSGSSQTEL PSPMDIAKSE 650
PAQMEVSLTG PPPVEPAQME PSPAKPPQVE APTYPQPPQR GPAPPTGPAP 700
PTGPAPPTEP APPTGLAEME PSPTEPSQKE PPPSMEPPCP EELPQAEPPP 750
MEDCQKELPS PVEPAQIEVA QTAPTQVQEE PPPVSEPPRV KPTKRSSLRK 800
DRAEKELSLL SEMARQEQVL MGVGLVPVRD SKLLKGNKSA QDPPAPPSPS 850
PKGNSREETP KDQEMVSDGE GTIVFPLKKG GPEEAGESPA ELAALKESAR 900
VSSSEQNSAM PEGGASHSKC QTGSSGLCDV DTEQKTDTVP MKDSAAEPVS 950
PPTPTVDRDA GSPAVVASPP ITLAENESQE IDEDEGIHSH DGSDLSDNMS 1000
EGSDDSGLHG ARPTPPEATS KNGKAGLAGK VTEGEFVCIF CDRSFRKEKD 1050
YSKHLNRHLV NVYFLEEAAE EQEEQEEREE QE 1082
Length:1,082
Mass (Da):117,784
Last modified:July 27, 2011 - v2
Checksum:i0D3616BC0820BC2F
GO
Isoform 2 (identifier: Q8VIG1-2) [UniParc]FASTAAdd to Basket

Also known as: N16, REST4

The sequence of this isoform differs from the canonical sequence as follows:
     323-328: GEKPFK → ECDLAG
     329-1082: Missing.

Show »
Length:328
Mass (Da):36,953
Checksum:iF47E141348260843
GO
Isoform 3 (identifier: Q8VIG1-3) [UniParc]FASTAAdd to Basket

Also known as: N28

The sequence of this isoform differs from the canonical sequence as follows:
     324-332: EKPFKCDQC → CDLVGYVFR
     333-1082: Missing.

Show »
Length:332
Mass (Da):37,475
Checksum:i15B8F53514610413
GO

Sequence cautioni

The sequence AAH96434.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei323 – 3286GEKPFK → ECDLAG in isoform 2.
VSP_022069
Alternative sequencei324 – 3329EKPFKCDQC → CDLVGYVFR in isoform 3.
VSP_022070
Alternative sequencei329 – 1082754Missing in isoform 2.
VSP_022071Add
BLAST
Alternative sequencei333 – 1082750Missing in isoform 3.
VSP_022072Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti414 – 4141T → R in BAB82460. 1 Publication
Sequence conflicti485 – 4851T → S in BAB82460. 1 Publication
Sequence conflicti556 – 5561G → S in BAB82460. 1 Publication
Sequence conflicti584 – 5841K → N in BAB82460. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024496 mRNA. Translation: BAB82460.1.
DQ644039 mRNA. Translation: ABG35129.1.
BC127065 mRNA. Translation: AAI27066.1.
BC096434 mRNA. Translation: AAH96434.1. Sequence problems.
AK004945 mRNA. Translation: BAB23689.3.
AK156514 mRNA. Translation: BAE33741.1.
CCDSiCCDS19372.1. [Q8VIG1-1]
RefSeqiNP_035393.2. NM_011263.2. [Q8VIG1-1]
XP_006534904.1. XM_006534841.1. [Q8VIG1-1]
XP_006534905.1. XM_006534842.1. [Q8VIG1-1]
UniGeneiMm.28840.
Mm.482359.

Genome annotation databases

EnsembliENSMUST00000080359; ENSMUSP00000079231; ENSMUSG00000029249. [Q8VIG1-1]
ENSMUST00000113449; ENSMUSP00000109076; ENSMUSG00000029249. [Q8VIG1-1]
GeneIDi19712.
KEGGimmu:19712.
UCSCiuc008xvz.2. mouse. [Q8VIG1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024496 mRNA. Translation: BAB82460.1 .
DQ644039 mRNA. Translation: ABG35129.1 .
BC127065 mRNA. Translation: AAI27066.1 .
BC096434 mRNA. Translation: AAH96434.1 . Sequence problems.
AK004945 mRNA. Translation: BAB23689.3 .
AK156514 mRNA. Translation: BAE33741.1 .
CCDSi CCDS19372.1. [Q8VIG1-1 ]
RefSeqi NP_035393.2. NM_011263.2. [Q8VIG1-1 ]
XP_006534904.1. XM_006534841.1. [Q8VIG1-1 ]
XP_006534905.1. XM_006534842.1. [Q8VIG1-1 ]
UniGenei Mm.28840.
Mm.482359.

3D structure databases

ProteinModelPortali Q8VIG1.
SMRi Q8VIG1. Positions 147-405.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202864. 6 interactions.
IntActi Q8VIG1. 1 interaction.
MINTi MINT-270819.

PTM databases

PhosphoSitei Q8VIG1.

Proteomic databases

PRIDEi Q8VIG1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000080359 ; ENSMUSP00000079231 ; ENSMUSG00000029249 . [Q8VIG1-1 ]
ENSMUST00000113449 ; ENSMUSP00000109076 ; ENSMUSG00000029249 . [Q8VIG1-1 ]
GeneIDi 19712.
KEGGi mmu:19712.
UCSCi uc008xvz.2. mouse. [Q8VIG1-1 ]

Organism-specific databases

CTDi 5978.
MGIi MGI:104897. Rest.

Phylogenomic databases

eggNOGi NOG271556.
GeneTreei ENSGT00530000063458.
HOGENOMi HOG000234631.
HOVERGENi HBG093893.
InParanoidi A0JNY8.
KOi K09222.
OMAi VQKEPVQ.
OrthoDBi EOG73FQM4.
TreeFami TF332861.

Miscellaneous databases

NextBioi 297094.
PROi Q8VIG1.
SOURCEi Search...

Gene expression databases

Bgeei Q8VIG1.
CleanExi MM_REST.
Genevestigatori Q8VIG1.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR027775. C2H2_Znf_fam.
IPR027757. REST.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
PANTHERi PTHR10032. PTHR10032. 1 hit.
PTHR10032:SF71. PTHR10032:SF71. 1 hit.
SMARTi SM00355. ZnF_C2H2. 9 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Alternative promoter and splicing generate multiple isoforms of the neural-restrictive silencer factor NRSF/REST,a general transcriptional repressor for multiple neuron specific genes."
    Kojima T., Naruse Y., Mori N.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: 129.
  2. "Cloning of the REST4 splice variant of the mouse REST protein."
    Puhl H.L. III, Ikeda S.R.
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Neuroblastoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-853 (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Liver and Spleen.
  5. "REST: a mammalian silencer protein that restricts sodium channel gene expression to neurons."
    Chong J.A., Tapia-Ramirez J., Kim S., Toledo-Aral J.J., Zheng Y., Boutros M.C., Altshuller Y.M., Frohman M.A., Kraner S.D., Mandel G.
    Cell 80:949-957(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes."
    Schoenherr C.J., Anderson D.J.
    Science 267:1360-1363(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Neuron-specific splicing of zinc finger transcription factor REST/NRSF/XBR is frequent in neuroblastomas and conserved in human, mouse and rat."
    Palm K., Metsis M., Timmusk T.
    Brain Res. Mol. Brain Res. 72:30-39(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORMS 2 AND 3).

Entry informationi

Entry nameiREST_MOUSE
AccessioniPrimary (citable) accession number: Q8VIG1
Secondary accession number(s): A0JNY8
, Q155C6, Q3U0W0, Q4VAD6, Q9CW43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi