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Q8VID6 (PDE11_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
EC=3.1.4.17
EC=3.1.4.35
Alternative name(s):
cAMP and cGMP phosphodiesterase 11A
Gene names
Name:Pde11a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length935 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively By similarity. Ref.1

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulation

Inhibited by 3-isobutyl-1-methylxanthine (IBMX), zaprinast and dipyridamole. cGMP acts as an allosteric activator. Ref.1

Subcellular location

Cytoplasm › cytosol By similarity.

Tissue specificity

Isoform 1 is expressed in brain, heart, kidney and liver, but not in prostate. Isoform 2 is specifically expressed in testis. Isoform 3 is expressed in various tissues including brain, lung, skeletal muscle, spleen, testis and prostate. Ref.1

Domain

The tandem GAF domains bind cGMP, and regulate enzyme activity. The binding of cGMP stimulates enzyme activity By similarity.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Biophysicochemical properties

Kinetic parameters:

KM=3.9 µM for cAMP (isoform 1) Ref.1

KM=1.6 µM for cGMP (isoform 1)

KM=4.0 µM for cAMP (isoform 2)

KM=1.6 µM for cGMP (isoform 2)

KM=2.2 µM for cAMP (isoform 3)

KM=1.3 µM for cGMP (isoform 3)

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VID6-1)

Also known as: PDE11A4;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VID6-2)

Also known as: PDE11A3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-250: Missing.
     251-304: KTLVSKFFDV...ETVNIPDAYQ → MLKQARRFSF...FLTRMQTRTK
Isoform 3 (identifier: Q8VID6-3)

Also known as: PDE11A2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-354: Missing.
     355-357: DEK → MSW

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 935935Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
PRO_0000247042

Regions

Domain217 – 370154GAF 1
Domain402 – 558157GAF 2
Region640 – 905266Catalytic By similarity

Sites

Active site6641Proton donor By similarity
Metal binding6681Divalent metal cation 1 By similarity
Metal binding7041Divalent metal cation 1 By similarity
Metal binding7051Divalent metal cation 1 By similarity
Metal binding7051Divalent metal cation 2 By similarity
Metal binding7081Divalent metal cation 2 By similarity
Metal binding7341Divalent metal cation 2 By similarity
Metal binding8161Divalent metal cation 1 By similarity
Binding site8691cAMP or cGMP By similarity

Amino acid modifications

Modified residue1621Phosphoserine By similarity
Modified residue2391Phosphoserine By similarity

Natural variations

Alternative sequence1 – 354354Missing in isoform 3.
VSP_019902
Alternative sequence1 – 250250Missing in isoform 2.
VSP_019903
Alternative sequence251 – 30454KTLVS…PDAYQ → MLKQARRFSFRNVRSATQWR KVGSTRQGQISGAFLAERLD KHQDFLTRMQTRTK in isoform 2.
VSP_019904
Alternative sequence355 – 3573DEK → MSW in isoform 3.
VSP_019905

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PDE11A4) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: E80F1039770F8276

FASTA935104,571
        10         20         30         40         50         60 
MAASRLDFGE VETFLDRHPE LFEDYLMRKG KQELVDKWLQ RHSSGQGASD LRPALAGASS 

        70         80         90        100        110        120 
LAQSSARGST GIGGGAGPQG SANSHPASGG GESAGVPLSP SWASGSRGDG NLQRRASQKE 

       130        140        150        160        170        180 
LRKSFARSKA IHVNRTYDEQ VTSRAQEPLS SVRRRALLRK ASSLPPTTAH ILSALLESRV 

       190        200        210        220        230        240 
NLPQYPPTAI DYKCHLKKHN ERQFFLELVK DISNDLDLTS LSYKILIFVC LMVDADRCSL 

       250        260        270        280        290        300 
FLVEGAAAGK KTLVSKFFDV HAGTPLLPCS TTENSNEVQV PWGKGIIGYV GEHGETVNIP 

       310        320        330        340        350        360 
DAYQDRRFND EIDKLTGYKT KSLLCMPIRN SDGEIIGVAQ AINKVPEGAP FTEDDEKVMQ 

       370        380        390        400        410        420 
MYLPFCGIAI SNAQLFAASR KEYERSRALL EVVNDLFEEQ TDLEKIVKKI MHRAQTLLKC 

       430        440        450        460        470        480 
ERCSVLLLED IESPVVKFTK SFELMSPKCS ADAENSFKES VEKSSYSDWL INNSIAELVA 

       490        500        510        520        530        540 
STGLPVNVSD AYQDPRFDAE ADQISGFHIR SVLCVPIWNS NHQIIGVAQV LNRLDGKPFD 

       550        560        570        580        590        600 
DADQRLFEAF VIFCGLGINN TIMYDQVKKS WAKQSVALDV LSYHATCSKA EVDKFKAANI 

       610        620        630        640        650        660 
PLVSELAIDD IHFDDFSLDV DAMITAALRM FMELGMVQKF KIDYETLCRW LLTVRKNYRM 

       670        680        690        700        710        720 
VLYHNWRHAF NVCQLMFAML TTAGFQEILT EVEILAVIVG CLCHDLDHRG TNNAFQAKSD 

       730        740        750        760        770        780 
SALAQLYGTS ATLEHHHFNH AVMILQSEGH NIFANLSSKE YSDLMQLLKQ SILATDLTLY 

       790        800        810        820        830        840 
FERRTEFFEL VSKGAYDWSI TSHRDVFRSM LMTACDLGAV TKPWEISRQV AELVTSEFFE 

       850        860        870        880        890        900 
QGDRERSELK LTPSAIFDRN RKDELPRLQL EWIDSICMPL YQALVKVNAK LKPMLDSVAA 

       910        920        930 
NRRKWEELHQ KRLQVSAASP VPSSPSPAVA GEDRL

« Hide

Isoform 2 (PDE11A3) [UniParc].

Checksum: 505E8748E9A6C21F
Show »

FASTA68578,067
Isoform 3 (PDE11A2) [UniParc].

Checksum: 2CA7C2F5DDB37D00
Show »

FASTA58166,142

References

[1]"Identification of rat cyclic nucleotide phosphodiesterase 11A (PDE11A): comparison of rat and human PDE11A splicing variants."
Yuasa K., Ohgaru T., Asahina M., Omori K.
Eur. J. Biochem. 268:4440-4448(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB059360 mRNA. Translation: BAB79627.1.
AB059361 mRNA. Translation: BAB79628.1.
AB059362 mRNA. Translation: BAB79629.1.
IPIIPI00363646.
IPI00515811.
IPI00561502.
RefSeqNP_001120952.1. NM_001127480.1.
NP_001120953.1. NM_001127481.2.
NP_543169.1. NM_080893.1.
UniGeneRn.88630.

3D structure databases

ProteinModelPortalQ8VID6.
ModBaseSearch...

PTM databases

PhosphoSiteQ8VID6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000008301; ENSRNOP00000008300; ENSRNOG00000024457.
ENSRNOT00000050355; ENSRNOP00000051289; ENSRNOG00000024457.
GeneID140928.
KEGGrno:140928.

Organism-specific databases

CTD50940.
RGD621793. Pde11a.

Phylogenomic databases

GeneTreeENSGT00690000102141.
HOGENOMHOG000007068.
HOVERGENHBG101207.
InParanoidQ8VID6.
KOK13298.

Gene expression databases

ArrayExpressQ8VID6.
GenevestigatorQ8VID6.
GermOnlineENSRNOG00000024457. Rattus norvegicus.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003018. GAF.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620787.

Entry information

Entry namePDE11_RAT
AccessionPrimary (citable) accession number: Q8VID6
Secondary accession number(s): Q8VID7, Q8VID8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2002
Last modified: April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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