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Protein

2'-5'-oligoadenylate synthase 3

Gene

Oas3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.1 Publication

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.

Cofactori

Mg2+Curated

Enzyme regulationi

Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated. ssRNA or dsDNA do not act as activators (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi867 – 8671Magnesium; catalyticSequence analysis
Metal bindingi869 – 8691Magnesium; catalyticSequence analysis
Metal bindingi939 – 9391Magnesium; catalyticSequence analysis
Binding sitei998 – 9981ATPBy similarity
Binding sitei998 – 9981SubstrateBy similarity

GO - Molecular functioni

  • 2'-5'-oligoadenylate synthetase activity Source: MGI
  • ATP binding Source: UniProtKB
  • double-stranded RNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 3 (EC:2.7.7.84)
Short name:
(2-5')oligo(A) synthase 3
Short name:
2-5A synthase 3
Alternative name(s):
2',5'-oligoadenylate synthetase-like 10
Gene namesi
Name:Oas3
Synonyms:oasl10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2180850. Oas3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 113811382'-5'-oligoadenylate synthase 3PRO_0000418630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8VI93.
MaxQBiQ8VI93.
PaxDbiQ8VI93.
PeptideAtlasiQ8VI93.
PRIDEiQ8VI93.

PTM databases

iPTMnetiQ8VI93.

Expressioni

Tissue specificityi

Intestine.1 Publication

Inductioni

By type I interferon (IFN) and viruses.

Gene expression databases

BgeeiQ8VI93.
ExpressionAtlasiQ8VI93. baseline and differential.
GenevisibleiQ8VI93. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi232935. 1 interaction.
STRINGi10090.ENSMUSP00000035588.

Structurei

3D structure databases

ProteinModelPortaliQ8VI93.
SMRiQ8VI93. Positions 1-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 341336OAS domain 1Add
BLAST
Regioni342 – 462121LinkerAdd
BLAST
Regioni463 – 793331OAS domain 2Add
BLAST
Regioni801 – 1135335OAS domain 3Add
BLAST

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00510000046406.
HOGENOMiHOG000013200.
HOVERGENiHBG007856.
InParanoidiQ8VI93.
KOiK14216.
OMAiCQGGEHA.
OrthoDBiEOG7WDN1R.
PhylomeDBiQ8VI93.
TreeFamiTF329749.

Family and domain databases

Gene3Di1.10.1410.20. 3 hits.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 3 hits.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 3 hits.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 2 hits.
PS50152. 25A_SYNTH_3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VI93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLFHTPAGA LDKLVAHNLH PAPEFTAAVR GALGSLNITL QQHRARGSQR
60 70 80 90 100
PRVIRIAKGG AYARGTALRG GTDVELVIFL DCFQSFGDQK TCHSETLGAM
110 120 130 140 150
RMLLESWGGH PGPGLTFEFS QSKASRILQF RLASADGEHW IDVSLVPAFD
160 170 180 190 200
VLGQPRSGVK PTPNVYSSLL SSHCQAGEYS ACFTEPRKNF VNTRPAKLKN
210 220 230 240 250
LILLVKHWYH QVQTRAVRAT LPPSYALELL TIFAWEQGCG KDSFSLAQGL
260 270 280 290 300
RTVLALIQHS KYLCIFWTEN YGFEDPAVGE FLRRQLKRPR PVILDPADPT
310 320 330 340 350
WDVGNGTAWR WDVLAQEAES SFSQQCFKQA SGVLVQPWEG PGLPRAGILD
360 370 380 390 400
LGHPIYQGPN QALEDNKGHL AVQSKERSQK PSNSAPGFPE AATKIPAMPN
410 420 430 440 450
PSANKTRKIR KKAAHPKTVQ EAALDSISSH VRITQSTASS HMPPDRSSIS
460 470 480 490 500
TAGSRMSPDL SQIPSKDLDC FIQDHLRPSP QFQQQVKQAI DAILCCLREK
510 520 530 540 550
SVYKVLRVSK GGSFGRGTDL RGSCDVELVI FYKTLGDFKG QKPHQAEILR
560 570 580 590 600
DMQAQLRHWC QNPVPGLSLQ FIEQKPNALQ LQLASTDLSN RVDLSVLPAF
610 620 630 640 650
DAVGPLKSGT KPQPQVYSSL LSSGCQAGEH AACFAELRRN FINTCPPKLK
660 670 680 690 700
SLMLLVKHWY RQVVTRYKGG EAAGDAPPPA YALELLTIFA WEQGCGEQKF
710 720 730 740 750
SLAEGLRTIL RLIQQHQSLC IYWTVNYSVQ DPAIRAHLLC QLRKARPLVL
760 770 780 790 800
DPADPTWNVG QGDWKLLAQE AAALGSQVCL QSGDGTLVPP WDVTPALLHQ
810 820 830 840 850
TLAEDLDKFI SEFLQPNRHF LTQVKRAVDT ICSFLKENCF RNSTIKVLKV
860 870 880 890 900
VKGGSSAKGT ALQGRSDADL VVFLSCFRQF SEQGSHRAEI ISEIQAHLEA
910 920 930 940 950
CQQMHSFDVK FEVSKRKNPR VLSFTLTSQT LLDQSVDFDV LPAFDALGQL
960 970 980 990 1000
RSGSRPDPRV YTDLIHSCSN AGEFSTCFTE LQRDFITSRP TKLKSLIRLV
1010 1020 1030 1040 1050
KYWYQQCNKT IKGKGSLPPQ HGLELLTVYA WEQGGQNPQF NMAEGFRTVL
1060 1070 1080 1090 1100
ELIVQYRQLC VYWTINYSAE DKTIGDFLKM QLRKPRPVIL DPADPTGNLG
1110 1120 1130
HNARWDLLAK EATVYASALC CVDRDGNPIK PWPVKAAV
Length:1,138
Mass (Da):126,333
Last modified:March 1, 2002 - v1
Checksum:i64568BA94FC51866
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2151R → Q in AAM47556 (PubMed:12080145).Curated
Sequence conflicti733 – 7331A → T in AAM47556 (PubMed:12080145).Curated
Sequence conflicti897 – 8971H → Q in AAM47556 (PubMed:12080145).Curated
Sequence conflicti897 – 8971H → Q in EDL19752 (Ref. 4) Curated
Sequence conflicti897 – 8971H → Q in AAI41056 (PubMed:15489334).Curated
Sequence conflicti904 – 9041M → T in AAM47556 (PubMed:12080145).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067534 mRNA. Translation: BAB84134.1.
AF453830 mRNA. Translation: AAM47556.1.
AC115937 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19752.1.
BC141055 mRNA. Translation: AAI41056.1.
CCDSiCCDS39242.1.
RefSeqiNP_660261.1. NM_145226.2.
UniGeneiMm.204887.

Genome annotation databases

EnsembliENSMUST00000044833; ENSMUSP00000035588; ENSMUSG00000032661.
GeneIDi246727.
KEGGimmu:246727.
UCSCiuc008zhz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067534 mRNA. Translation: BAB84134.1.
AF453830 mRNA. Translation: AAM47556.1.
AC115937 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19752.1.
BC141055 mRNA. Translation: AAI41056.1.
CCDSiCCDS39242.1.
RefSeqiNP_660261.1. NM_145226.2.
UniGeneiMm.204887.

3D structure databases

ProteinModelPortaliQ8VI93.
SMRiQ8VI93. Positions 1-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232935. 1 interaction.
STRINGi10090.ENSMUSP00000035588.

PTM databases

iPTMnetiQ8VI93.

Proteomic databases

EPDiQ8VI93.
MaxQBiQ8VI93.
PaxDbiQ8VI93.
PeptideAtlasiQ8VI93.
PRIDEiQ8VI93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044833; ENSMUSP00000035588; ENSMUSG00000032661.
GeneIDi246727.
KEGGimmu:246727.
UCSCiuc008zhz.1. mouse.

Organism-specific databases

CTDi4940.
MGIiMGI:2180850. Oas3.

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00510000046406.
HOGENOMiHOG000013200.
HOVERGENiHBG007856.
InParanoidiQ8VI93.
KOiK14216.
OMAiCQGGEHA.
OrthoDBiEOG7WDN1R.
PhylomeDBiQ8VI93.
TreeFamiTF329749.

Miscellaneous databases

PROiQ8VI93.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VI93.
ExpressionAtlasiQ8VI93. baseline and differential.
GenevisibleiQ8VI93. MM.

Family and domain databases

Gene3Di1.10.1410.20. 3 hits.
InterProiIPR006117. 2-5-oligoadenylate_synth_CS.
IPR006116. 2-5-oligoadenylate_synth_N.
IPR018952. 2-5-oligoAdlate_synth_1_dom2/C.
IPR026774. 2-5A_synthase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR11258. PTHR11258. 3 hits.
PfamiPF01909. NTP_transf_2. 1 hit.
PF10421. OAS1_C. 3 hits.
[Graphical view]
PROSITEiPS00832. 25A_SYNTH_1. 1 hit.
PS00833. 25A_SYNTH_2. 2 hits.
PS50152. 25A_SYNTH_3. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic structure of the mouse 2',5'-oligoadenylate synthetase gene family."
    Kakuta S., Shibata S., Iwakura Y.
    J. Interferon Cytokine Res. 22:981-993(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Colon.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/RV.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "The mammalian 2'-5' oligoadenylate synthetase gene family: evidence for concerted evolution of paralogous Oas1 genes in Rodentia and Artiodactyla."
    Perelygin A.A., Zharkikh A.A., Scherbik S.V., Brinton M.A.
    J. Mol. Evol. 63:562-576(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.
  8. "The oligoadenylate synthetase family: an ancient protein family with multiple antiviral activities."
    Kristiansen H., Gad H.H., Eskildsen-Larsen S., Despres P., Hartmann R.
    J. Interferon Cytokine Res. 31:41-47(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION.

Entry informationi

Entry nameiOAS3_MOUSE
AccessioniPrimary (citable) accession number: Q8VI93
Secondary accession number(s): B9EIU4, Q8K4D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.