Q8VI82 (FGF23_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fibroblast growth factor 23
Short name=FGF-23

Gene names

Name:

Fgf23

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulator of phosphate homeostasis By similarity. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels By similarity. Regulator of vitamin-D metabolism By similarity. Negatively regulates osteoblasts differentiation and matrix mineralization By similarity. Acts directly on the parathyroid to decrease PTH secretion. Upregulates EGR1 expression in the presence of KL. Ref.2 Ref.3
Subunit structure	Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL and heparan sulfate glycosaminoglycans that function as coreceptors By similarity.
Subcellular location	Secreted By similarity. Note: Secretion is dependent on O-glycosylation By similarity.
Tissue specificity	Expressed in the parathyroid. Ref.3
Post-translational modification	Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases By similarity. O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23 By similarity.
Sequence similarities	Belongs to the heparin-binding growth factors family.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cellular phosphate ion homeostasis Inferred from electronic annotation. Source: Compara negative regulation of bone mineralization Inferred from electronic annotation. Source: Compara negative regulation of hormone secretion Inferred from direct assay Ref.3. Source: UniProtKB negative regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara phosphate-containing compound metabolic process Inferred from expression pattern PubMed 15531762. Source: RGD positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of vitamin D 24-hydroxylase activity Inferred from electronic annotation. Source: Compara regulation of phosphate transport Inferred from electronic annotation. Source: Compara vitamin D catabolic process Inferred from electronic annotation. Source: Compara vitamin D metabolic process Inferred from expression pattern PubMed 15531762. Source: RGD
Cellular_component	extracellular region part Inferred from direct assay PubMed 15531762. Source: RGD
Molecular_function	fibroblast growth factor receptor binding Traceable author statement PubMed 15531762. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 251	227	Fibroblast growth factor 23		PRO_0000009000
Amino acid modifications
	Glycosylation	178	1	O-linked (GalNAc) By similarity
	Disulfide bond	95 ↔ 113		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VI82 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 35A229E1B3900593	FASTA	251	27,912
	10 20 30 40 50 60 MLGACLRLLV GALCTVCSLG TARAYSDTSP LLGSNWGSLT HLYTATARNS YHLQIHRDGH 70 80 90 100 110 120 VDGTPHQTIY SALMITSEDA GSVVIIGAMT RRFLCMDLRG NIFGSYHFSP ENCRFRQWTL 130 140 150 160 170 180 ENGYDVYLSP KHHYLVSLGR SKRIFQPGTN PPPFSQFLAR RNEVPLLHFY TARPRRHTRS 190 200 210 220 230 240 AEDPPERDPL NVLKPRPRAT PIPVSCSREL PSAEEGGPAA SDPLGVLRRG RGDARRGAGG 250 TDRCRPFPRF V

References

[1]	"Rattus norvegicus fgf23." Itoh N. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Klotho converts canonical FGF receptor into a specific receptor for FGF23." Urakawa I., Yamazaki Y., Shimada T., Iijima K., Hasegawa H., Okawa K., Fujita T., Fukumoto S., Yamashita T. Nature 444:770-774(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"The parathyroid is a target organ for FGF23 in rats." Ben-Dov I.Z., Galitzer H., Lavi-Moshayoff V., Goetz R., Kuro-o M., Mohammadi M., Sirkis R., Naveh-Many T., Silver J. J. Clin. Invest. 117:4003-4008(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB078777 mRNA. Translation: BAB84108.1.
IPI	IPI00207491.
RefSeq	NP_570110.1. NM_130754.1.
UniGene	Rn.154421.
3D structure databases
ProteinModelPortal	Q8VI82.
SMR	Q8VI82. Positions 29-169.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000026888.
Proteomic databases
PRIDE	Q8VI82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026888; ENSRNOP00000026888; ENSRNOG00000019855.
GeneID	170583.
KEGG	rno:170583.
UCSC	RGD:620178. rat.
Organism-specific databases
CTD	8074.
RGD	620178. Fgf23.
Phylogenomic databases
eggNOG	NOG270405.
GeneTree	ENSGT00530000063469.
HOGENOM	HOG000112573.
HOVERGEN	HBG051613.
InParanoid	Q8VI82.
KO	K04358.
OMA	NTPRPRR.
OrthoDB	EOG4MKNH6.
Gene expression databases
Genevestigator	Q8VI82.
GermOnline	ENSRNOG00000019855. Rattus norvegicus.
Family and domain databases
InterPro	IPR008996. Cytokine_IL1-like. IPR002348. IL1_HBGF. [Graphical view]
PANTHER	PTHR11486. PTHR11486. 1 hit.
Pfam	PF00167. FGF. 1 hit. [Graphical view]
SMART	SM00442. FGF. 1 hit. [Graphical view]
SUPFAM	SSF50353. Cytok_IL1_like. 1 hit.
PROSITE	PS00247. HBGF_FGF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	621084.

Entry information

Entry name

FGF23_RAT

Accession

Primary (citable) accession number: Q8VI82

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program