Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8VI67

- SP7_MOUSE

UniProt

Q8VI67 - SP7_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcription factor Sp7

Gene

Sp7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri291 – 31525C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri321 – 34525C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri351 – 37323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DEAD/H-box RNA helicase binding Source: BHF-UCL
  2. DNA binding Source: MGI
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. hematopoietic stem cell differentiation Source: Ensembl
  2. osteoblast differentiation Source: MGI
  3. positive regulation of stem cell differentiation Source: Ensembl
  4. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. regulation of transcription from RNA polymerase II promoter Source: MGI
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Sp7
Alternative name(s):
C22
Zinc finger protein osterix
Gene namesi
Name:Sp7
Synonyms:Osx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2153568. Sp7.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Death in the immediate postnatal period due to difficulty in breathing. Mice rapidly become cyanotic and die within 15 min of birth. New-born homozygous show severe inward bending of forelimbs and hindlimbs. They develop a normal cartilage skeleton but fail to form bone and to express osteoblast-specific marker genes. In endochondral skeletal elements, mesenchymal cells together with osteoclasts and blood vessels, invade the mineralized cartilage matrix.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Transcription factor Sp7PRO_0000047151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki55 – 55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki227 – 227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitination at leads to proteasomal degradation. SP7 is a short-live protein with an endogenous half-life of approximately 12 hours (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiQ8VI67.

PTM databases

PhosphoSiteiQ8VI67.

Expressioni

Tissue specificityi

Osteoblast/chondrocyte specific.

Gene expression databases

BgeeiQ8VI67.
CleanExiMM_SP7.
ExpressionAtlasiQ8VI67. baseline.
GenevestigatoriQ8VI67.

Interactioni

Subunit structurei

Interacts with NO66; the interaction is direct and inhibits transcription activator activity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NO66Q9H6W36EBI-7608836,EBI-2513645From a different organism.
No66Q9JJF33EBI-7608836,EBI-7608809

Protein-protein interaction databases

BioGridi228354. 6 interactions.
DIPiDIP-46090N.
IntActiQ8VI67. 4 interactions.
MINTiMINT-7709305.
STRINGi10090.ENSMUSP00000077596.

Structurei

3D structure databases

ProteinModelPortaliQ8VI67.
SMRiQ8VI67. Positions 287-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri291 – 31525C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri321 – 34525C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri351 – 37323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118984.
HOGENOMiHOG000231067.
HOVERGENiHBG036899.
InParanoidiQ8VI67.
KOiK09197.
OMAiPKTMGDA.
OrthoDBiEOG73FQMR.
PhylomeDBiQ8VI67.
TreeFamiTF350150.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VI67-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD
60 70 80 90 100
LSAPKTMGDA YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT
110 120 130 140 150
GAQDPGLLVP KGHSSSDCLP SVYTSLDMTH PYGSWYKAGI HAGISPGPGN
160 170 180 190 200
TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS TGPAQPPLNP QLPTYPSDFA
210 220 230 240 250
PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS GAAKPPRGAG
260 270 280 290 300
TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK
310 320 330 340 350
VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK
360 370 380 390 400
FTCLLCSKRF TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN
410 420
QPPRSSTSPA PPEKAHGGSP EQSNLLEI
Length:428
Mass (Da):44,718
Last modified:March 1, 2002 - v1
Checksum:iB794988958743586
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641P → L in BAC36263. (PubMed:16141072)Curated
Sequence conflicti130 – 1301H → Y in BAC36774. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184902 mRNA. Translation: AAL60067.1.
AK032521 mRNA. Translation: BAC27908.1.
AK076229 mRNA. Translation: BAC36263.1.
AK077375 mRNA. Translation: BAC36774.1.
CCDSiCCDS37228.1.
RefSeqiNP_569725.1. NM_130458.3.
XP_006520582.1. XM_006520519.1.
UniGeneiMm.263284.

Genome annotation databases

EnsembliENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284.
GeneIDi170574.
KEGGimmu:170574.
UCSCiuc007xvm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184902 mRNA. Translation: AAL60067.1 .
AK032521 mRNA. Translation: BAC27908.1 .
AK076229 mRNA. Translation: BAC36263.1 .
AK077375 mRNA. Translation: BAC36774.1 .
CCDSi CCDS37228.1.
RefSeqi NP_569725.1. NM_130458.3.
XP_006520582.1. XM_006520519.1.
UniGenei Mm.263284.

3D structure databases

ProteinModelPortali Q8VI67.
SMRi Q8VI67. Positions 287-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 228354. 6 interactions.
DIPi DIP-46090N.
IntActi Q8VI67. 4 interactions.
MINTi MINT-7709305.
STRINGi 10090.ENSMUSP00000077596.

PTM databases

PhosphoSitei Q8VI67.

Proteomic databases

PRIDEi Q8VI67.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000078508 ; ENSMUSP00000077596 ; ENSMUSG00000060284 .
GeneIDi 170574.
KEGGi mmu:170574.
UCSCi uc007xvm.1. mouse.

Organism-specific databases

CTDi 121340.
MGIi MGI:2153568. Sp7.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00760000118984.
HOGENOMi HOG000231067.
HOVERGENi HBG036899.
InParanoidi Q8VI67.
KOi K09197.
OMAi PKTMGDA.
OrthoDBi EOG73FQMR.
PhylomeDBi Q8VI67.
TreeFami TF350150.

Miscellaneous databases

NextBioi 370123.
PROi Q8VI67.
SOURCEi Search...

Gene expression databases

Bgeei Q8VI67.
CleanExi MM_SP7.
ExpressionAtlasi Q8VI67. baseline.
Genevestigatori Q8VI67.

Family and domain databases

Gene3Di 3.30.160.60. 3 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 3 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation."
    Nakashima K., Zhou X., Kunkel G., Zhang Z., Deng J.M., Behringer R.R., de Crombrugghe B.
    Cell 108:17-29(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Olfactory neuron.
  3. "Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase."
    Sinha K.M., Yasuda H., Coombes M.M., Dent S.Y., de Crombrugghe B.
    EMBO J. 29:68-79(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NO66.

Entry informationi

Entry nameiSP7_MOUSE
AccessioniPrimary (citable) accession number: Q8VI67
Secondary accession number(s): Q8C5R3, Q8C6A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3