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Q8VI67 (SP7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor Sp7
Alternative name(s):
C22
Zinc finger protein osterix
Gene names
Name:Sp7
Synonyms:Osx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences. Ref.1

Subunit structure

Interacts with NO66; the interaction is direct and inhibits transcription activator activity. Ref.3

Subcellular location

Nucleus Ref.1.

Tissue specificity

Osteoblast/chondrocyte specific.

Disruption phenotype

Death in the immediate postnatal period due to difficulty in breathing. Mice rapidly become cyanotic and die within 15 min of birth. New-born homozygous show severe inward bending of forelimbs and hindlimbs. They develop a normal cartilage skeleton but fail to form bone and to express osteoblast-specific marker genes. In endochondral skeletal elements, mesenchymal cells together with osteoclasts and blood vessels, invade the mineralized cartilage matrix. Ref.1

Sequence similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NO66Q9H6W36EBI-7608836,EBI-2513645From a different organism.
No66Q9JJF33EBI-7608836,EBI-7608809

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Transcription factor Sp7
PRO_0000047151

Regions

Zinc finger291 – 31525C2H2-type 1
Zinc finger321 – 34525C2H2-type 2
Zinc finger351 – 37323C2H2-type 3

Experimental info

Sequence conflict641P → L in BAC36263. Ref.2
Sequence conflict1301H → Y in BAC36774. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8VI67 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: B794988958743586

FASTA42844,718
        10         20         30         40         50         60 
MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD LSAPKTMGDA 

        70         80         90        100        110        120 
YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT GAQDPGLLVP KGHSSSDCLP 

       130        140        150        160        170        180 
SVYTSLDMTH PYGSWYKAGI HAGISPGPGN TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS 

       190        200        210        220        230        240 
TGPAQPPLNP QLPTYPSDFA PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS 

       250        260        270        280        290        300 
GAAKPPRGAG TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK 

       310        320        330        340        350        360 
VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK FTCLLCSKRF 

       370        380        390        400        410        420 
TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN QPPRSSTSPA PPEKAHGGSP 


EQSNLLEI 

« Hide

References

« Hide 'large scale' references
[1]"The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation."
Nakashima K., Zhou X., Kunkel G., Zhang Z., Deng J.M., Behringer R.R., de Crombrugghe B.
Cell 108:17-29(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head and Olfactory neuron.
[3]"Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase."
Sinha K.M., Yasuda H., Coombes M.M., Dent S.Y., de Crombrugghe B.
EMBO J. 29:68-79(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NO66.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF184902 mRNA. Translation: AAL60067.1.
AK032521 mRNA. Translation: BAC27908.1.
AK076229 mRNA. Translation: BAC36263.1.
AK077375 mRNA. Translation: BAC36774.1.
RefSeqNP_569725.1. NM_130458.3.
XP_006520582.1. XM_006520519.1.
UniGeneMm.263284.

3D structure databases

ProteinModelPortalQ8VI67.
SMRQ8VI67. Positions 254-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid228354. 6 interactions.
DIPDIP-46090N.
IntActQ8VI67. 4 interactions.
MINTMINT-7709305.
STRING10090.ENSMUSP00000077596.

PTM databases

PhosphoSiteQ8VI67.

Proteomic databases

PRIDEQ8VI67.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284.
GeneID170574.
KEGGmmu:170574.
UCSCuc007xvm.1. mouse.

Organism-specific databases

CTD121340.
MGIMGI:2153568. Sp7.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00750000117314.
HOGENOMHOG000231067.
HOVERGENHBG036899.
InParanoidQ8VI67.
KOK09197.
OMAPKTMGDA.
OrthoDBEOG73FQMR.
PhylomeDBQ8VI67.
TreeFamTF350150.

Gene expression databases

BgeeQ8VI67.
CleanExMM_SP7.
GenevestigatorQ8VI67.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio370123.
PROQ8VI67.
SOURCESearch...

Entry information

Entry nameSP7_MOUSE
AccessionPrimary (citable) accession number: Q8VI67
Secondary accession number(s): Q8C5R3, Q8C6A7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot