Q8VI67 (SP7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor Sp7

Alternative name(s):
C22
Zinc finger protein osterix

Gene names

Name:	Sp7
Synonyms:	Osx

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences. Ref.1
Subunit structure	Interacts with NO66; the interaction is direct and inhibits transcription activator activity. Ref.3
Subcellular location	Nucleus Ref.1.
Tissue specificity	Osteoblast/chondrocyte specific.
Disruption phenotype	Death in the immediate postnatal period due to difficulty in breathing. Mice rapidly become cyanotic and die within 15 min of birth. New-born homozygous show severe inward bending of forelimbs and hindlimbs. They develop a normal cartilage skeleton but fail to form bone and to express osteoblast-specific marker genes. In endochondral skeletal elements, mesenchymal cells together with osteoclasts and blood vessels, invade the mineralized cartilage matrix. Ref.1
Sequence similarities	Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hematopoietic stem cell differentiation Inferred from electronic annotation. Source: Compara osteoblast differentiation Inferred from mutant phenotype Ref.1. Source: MGI positive regulation of stem cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	DNA binding Inferred from direct assay Ref.1. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Transcription factor Sp7		PRO_0000047151
Regions
	Zinc finger	291 – 315	25	C2H2-type 1
	Zinc finger	321 – 345	25	C2H2-type 2
	Zinc finger	351 – 373	23	C2H2-type 3
Experimental info
	Sequence conflict	64	1	P → L in BAC36263. Ref.2
	Sequence conflict	130	1	H → Y in BAC36774. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8VI67 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: B794988958743586	FASTA	428	44,718
	10 20 30 40 50 60 MASSLLEEEA HYGSSPLAML TAACSKFGGS SPLRDSTTLG KGGTKKPYAD LSAPKTMGDA 70 80 90 100 110 120 YPAPFSSTNG LLSPAGSPPA PASGYANDYP PFPHSFPGPT GAQDPGLLVP KGHSSSDCLP 130 140 150 160 170 180 SVYTSLDMTH PYGSWYKAGI HAGISPGPGN TPTPWWDMHP GGNWLGGGQG QGDGLQGTLS 190 200 210 220 230 240 TGPAQPPLNP QLPTYPSDFA PLNPAPYPAP HLLQPGPQHV LPQDVYKPKA VGNSGQLEGS 250 260 270 280 290 300 GAAKPPRGAG TGGSGGYAGS GAGRSTCDCP NCQELERLGA AAAGLRKKPI HSCHIPGCGK 310 320 330 340 350 360 VYGKASHLKA HLRWHTGERP FVCNWLFCGK RFTRSDELER HVRTHTREKK FTCLLCSKRF 370 380 390 400 410 420 TRSDHLSKHQ RTHGEPGPGP PPSGPKELGE GRSVGEEEAN QPPRSSTSPA PPEKAHGGSP EQSNLLEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation." Nakashima K., Zhou X., Kunkel G., Zhang Z., Deng J.M., Behringer R.R., de Crombrugghe B. Cell 108:17-29(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head and Olfactory neuron.
[3]	"Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase." Sinha K.M., Yasuda H., Coombes M.M., Dent S.Y., de Crombrugghe B. EMBO J. 29:68-79(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NO66.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF184902 mRNA. Translation: AAL60067.1. AK032521 mRNA. Translation: BAC27908.1. AK076229 mRNA. Translation: BAC36263.1. AK077375 mRNA. Translation: BAC36774.1.
IPI	IPI00128835.
RefSeq	NP_569725.1. NM_130458.3.
UniGene	Mm.263284.
3D structure databases
ProteinModelPortal	Q8VI67.
SMR	Q8VI67. Positions 262-379.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46090N.
STRING	10090.ENSMUSP00000077596.
PTM databases
PhosphoSite	Q8VI67.
Proteomic databases
PRIDE	Q8VI67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000078508; ENSMUSP00000077596; ENSMUSG00000060284.
GeneID	170574.
KEGG	mmu:170574.
Organism-specific databases
CTD	121340.
MGI	MGI:2153568. Sp7.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00670000097746.
HOGENOM	HOG000231067.
HOVERGEN	HBG036899.
InParanoid	Q8VI67.
KO	K09197.
OMA	YPAPFSS.
OrthoDB	EOG4FTW0R.
Gene expression databases
ArrayExpress	Q8VI67.
Bgee	Q8VI67.
CleanEx	MM_SP7.
Genevestigator	Q8VI67.
GermOnline	ENSMUSG00000060284. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	370123.
SOURCE	Search...

Entry information

Entry name

SP7_MOUSE

Accession

Primary (citable) accession number: Q8VI67
Secondary accession number(s): Q8C5R3, Q8C6A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program