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Protein

Extracellular sulfatase Sulf-1

Gene

Sulf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the desulfation of sulfated glycosaminoglycans in the biosynthetic pathway and may play a role as an extracellular regulator of proteoglycan mediated signaling.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511CalciumBy similarity
Metal bindingi52 – 521CalciumBy similarity
Metal bindingi87 – 871Calcium; via 3-oxoalanineBy similarity
Metal bindingi316 – 3161CalciumBy similarity
Metal bindingi317 – 3171CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular sulfatase Sulf-1 (EC:3.1.6.-)
Alternative name(s):
RSulfFP1
Sulfatase FP
Gene namesi
Name:Sulf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708554. Sulf1.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • extracellular space Source: UniProtKB
  • Golgi apparatus Source: RGD
  • Golgi stack Source: UniProtKB
  • membrane raft Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 870848Extracellular sulfatase Sulf-1PRO_0000033436Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence analysis
Modified residuei87 – 8713-oxoalanine (Cys)By similarity
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence analysis
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence analysis
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence analysis
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence analysis
Glycosylationi772 – 7721N-linked (GlcNAc...)Sequence analysis
Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VI60.

Expressioni

Tissue specificityi

Strongly expressed in the floor plate, choroid plexus and cartilage. Weaker expression seen in the eye, lung brain, spinal cord, urinary bladder, testis, ovary and uterus.

Developmental stagei

Abundantly and specifically expressed in the floor plate region of the spinal cord at embryonic day 13 (E13) and E15 and at lower levels at postnatal day 1 (P1). A high level expression was also observed in the ventral hindbrain and midbrain. No expression was seen in the notochord at these stages. Prominent expression in the nervous system was also seen in the choroid plexus. At E13, expression was also seen in the dorsomedial forebrain and roof of the hind brain. Robust and specific expression was detected in the choroid plexus epithelium of the lateral, 3rd and 4th ventricles at E15 and P1 and persisted into adulthood. In the CNS, expression was detected in thalamic and hypothalamic regions at E13, E15 and P1. In embryos, it was highly expressed in the limb buds, face region, large blood vessels, olfactory pits and pleural epithelium. In particular, expression was robust and specific in bone-forming regions of the face and in the mesenchymal cells surrounding the developing cartilages of the extremities.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012611.

Structurei

3D structure databases

ProteinModelPortaliQ8VI60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3731. Eukaryota.
COG3119. LUCA.
HOGENOMiHOG000290161.
HOVERGENiHBG056431.
InParanoidiQ8VI60.
KOiK14607.
PhylomeDBiQ8VI60.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR014615. Extracellular_sulfatase.
IPR024609. Extracellular_sulfatase_C.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF12548. DUF3740. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036665. Sulf1. 1 hit.
SUPFAMiSSF53649. SSF53649. 3 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VI60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNSCWALLL AVLGAELLGG FCSTMRSQRF RGRVQQERKN IRPNIILVLT
60 70 80 90 100
DDQDVELGSL QVMNKTRKIM EHGGATFTNA FVTTPMCCPS RSSMLTGKYV
110 120 130 140 150
HNHNVYTNNE NCSSPSWQAL HEPRTFAVYL NNTGYRTAFF GKYLNEYNGS
160 170 180 190 200
YIPPGWREWL GLIKNSRFYN YTVCRNGIKE KHGFDYAKDY FTDLITNESI
210 220 230 240 250
NYFKMSKRMY PHRPVMMVIS HAAPHGPEDS APQFSKLYPN ASQHITPSYN
260 270 280 290 300
YAPNMDKHWI MQYTGPMLPI HMEFTNVLQR KRLQTLMSVD DSVERLYNML
310 320 330 340 350
VETGELGNTY IIYTADHGYH IGQFGLVKGK SMPYDFDIRV PFFIRGPSIE
360 370 380 390 400
PGSIVPQIVL NIDLAPTILD IAGLDTPSDV DGKSVLKLLD LEKPGNRFRT
410 420 430 440 450
NKKAKIWRDT FLVERGKFLR KKEESSKNIQ QSNHLPKYER VKELCQQARY
460 470 480 490 500
QTACEQPGQN WQCIEDTSGK LRIHKCKGPS DLLTVRQNAR NLYSRGLQDK
510 520 530 540 550
DKECHCRESG YRPSRSQRKK ERQFLRNQGT PKYKPRFVHT RQTRSLSVEF
560 570 580 590 600
EGEIYDINLE EEELQVLPPR SVAKRHDEGH QGFGGHQAAA GDFRNEMLAD
610 620 630 640 650
SNSAVGLPTT VRVTHKCFIL PNDTIHCERE LYQSARAWKD HKAYIDKEIE
660 670 680 690 700
VLQDKIKNLR EVRGHLKKRK PEECSCGKQS YYNKEKGVKR QEKLKSHLHP
710 720 730 740 750
FKEAAAQEVD SKLQLFKEHR RRKKERKEKK RQRKGEECSL PGLTCFTHDN
760 770 780 790 800
NHWQTAPFWN LGSFCACTSS NNNTYWCLRT VNETHNFLFC EFATGFLEYF
810 820 830 840 850
DMNTDPYQLT NTVHTVERGI LNQLHIQLME LRSCQGYKQC NPRPKSLDVG
860 870
TKEGGNYDPH RGQLWDGWEG
Length:870
Mass (Da):100,860
Last modified:March 1, 2002 - v1
Checksum:i037813868C08967F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230072 mRNA. Translation: AAL71906.1.
RefSeqiNP_599205.1. NM_134378.2.
UniGeneiRn.161961.

Genome annotation databases

GeneIDi171396.
KEGGirno:171396.
UCSCiRGD:708554. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230072 mRNA. Translation: AAL71906.1.
RefSeqiNP_599205.1. NM_134378.2.
UniGeneiRn.161961.

3D structure databases

ProteinModelPortaliQ8VI60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012611.

Proteomic databases

PaxDbiQ8VI60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171396.
KEGGirno:171396.
UCSCiRGD:708554. rat.

Organism-specific databases

CTDi23213.
RGDi708554. Sulf1.

Phylogenomic databases

eggNOGiKOG3731. Eukaryota.
COG3119. LUCA.
HOGENOMiHOG000290161.
HOVERGENiHBG056431.
InParanoidiQ8VI60.
KOiK14607.
PhylomeDBiQ8VI60.

Miscellaneous databases

PROiQ8VI60.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR014615. Extracellular_sulfatase.
IPR024609. Extracellular_sulfatase_C.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF12548. DUF3740. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036665. Sulf1. 1 hit.
SUPFAMiSSF53649. SSF53649. 3 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of a novel nonlysosomal sulphatase expressed in the floor plate, choroid plexus and cartilage."
    Ohto T., Uchida H., Yamazaki H., Keino-Masu K., Matsui A., Masu M.
    Genes Cells 7:173-185(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Embryo.
  2. Erratum
    Ohto T., Uchida H., Yamazaki H., Keino-Masu K., Matsui A., Masu M.
    Genes Cells 7:521-522(2002)

Entry informationi

Entry nameiSULF1_RAT
AccessioniPrimary (citable) accession number: Q8VI60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2002
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.