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Q8VI38

- GBGT1_MOUSE

UniProt

Q8VI38 - GBGT1_MOUSE

Protein

Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

Gene

Gbgt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of Forssman glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to GalNAcb-1,3Gala-1,4Galb-1,4GlcCer (Gb4Cer). Forssman glycolipid (also called Forssman antigen; FG) probably serves for adherence of some pathogens. Conversely, it diminishes Shiga toxins susceptibility.1 Publication

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide.

    Cofactori

    Manganese.By similarity

    Pathwayi

    GO - Molecular functioni

    1. globoside alpha-N-acetylgalactosaminyltransferase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. lipid glycosylation Source: UniProtKB
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT6. Glycosyltransferase Family 6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (EC:2.4.1.88)
    Alternative name(s):
    Forssman glycolipid synthase
    Gene namesi
    Name:Gbgt1
    Synonyms:Fgs
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2449143. Gbgt1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 347347Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1PRO_0000157296Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8VI38.

    Expressioni

    Gene expression databases

    BgeeiQ8VI38.
    GenevestigatoriQ8VI38.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VI38.
    SMRiQ8VI38. Positions 63-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini28 – 347320LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis By similarity.By similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43612.
    GeneTreeiENSGT00400000022032.
    HOGENOMiHOG000234339.
    HOVERGENiHBG003563.
    InParanoidiQ8VI38.
    KOiK00722.
    OMAiPCPEIFN.
    OrthoDBiEOG72JWGX.
    PhylomeDBiQ8VI38.
    TreeFamiTF330991.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10462. PTHR10462. 1 hit.
    PfamiPF03414. Glyco_transf_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8VI38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRPRLAQGL AFFLLGGTGL WVLWKFIKDW LLVSYIPYYL PCPEFFNMKL    50
    PFRKEKPLQP VTQLQYPQPK LLEHGPTELL TLTPWLAPIV SEGTFDPELL 100
    KSMYQPLNLT IGVTVFAVGK YTCFIQRFLE SAEEFFMRGY QVHYYLFTHD 150
    PTAVPRVPLG PGRLLSIIPI QGYSRWEEIS MRRMETINKH IAKRAHKEVD 200
    YLFCVDVDMV FRNPWGPETL GDLVAAIHPG YFAVPRRKFP YERRQVSSAF 250
    VADNEGDFYY GGALFGGRVA RVYEFTRACH MAILADKANS IMAAWQEESH 300
    LNRHFIWHKP SKVLSPEYLW DERKPRPRSL KMIRFSSVKK NANWLRT 347
    Length:347
    Mass (Da):40,502
    Last modified:July 10, 2007 - v2
    Checksum:i69C7E141334A5F44
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti337 – 3371S → P in AAL37034. (PubMed:14573676)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF292399 mRNA. Translation: AAL37034.1.
    AB084789 mRNA. Translation: BAC23060.2.
    AL772249 Genomic DNA. Translation: CAM24089.1.
    BC106831 mRNA. Translation: AAI06832.1.
    CCDSiCCDS15839.1.
    RefSeqiNP_631936.2. NM_139197.2.
    XP_006497981.1. XM_006497918.1.
    XP_006497982.1. XM_006497919.1.
    XP_006497983.1. XM_006497920.1.
    UniGeneiMm.484270.

    Genome annotation databases

    EnsembliENSMUST00000028172; ENSMUSP00000028172; ENSMUSG00000026829.
    ENSMUST00000163121; ENSMUSP00000127071; ENSMUSG00000026829.
    GeneIDi227671.
    KEGGimmu:227671.
    UCSCiuc008iyl.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF292399 mRNA. Translation: AAL37034.1 .
    AB084789 mRNA. Translation: BAC23060.2 .
    AL772249 Genomic DNA. Translation: CAM24089.1 .
    BC106831 mRNA. Translation: AAI06832.1 .
    CCDSi CCDS15839.1.
    RefSeqi NP_631936.2. NM_139197.2.
    XP_006497981.1. XM_006497918.1.
    XP_006497982.1. XM_006497919.1.
    XP_006497983.1. XM_006497920.1.
    UniGenei Mm.484270.

    3D structure databases

    ProteinModelPortali Q8VI38.
    SMRi Q8VI38. Positions 63-338.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT6. Glycosyltransferase Family 6.

    Proteomic databases

    PRIDEi Q8VI38.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028172 ; ENSMUSP00000028172 ; ENSMUSG00000026829 .
    ENSMUST00000163121 ; ENSMUSP00000127071 ; ENSMUSG00000026829 .
    GeneIDi 227671.
    KEGGi mmu:227671.
    UCSCi uc008iyl.2. mouse.

    Organism-specific databases

    CTDi 26301.
    MGIi MGI:2449143. Gbgt1.

    Phylogenomic databases

    eggNOGi NOG43612.
    GeneTreei ENSGT00400000022032.
    HOGENOMi HOG000234339.
    HOVERGENi HBG003563.
    InParanoidi Q8VI38.
    KOi K00722.
    OMAi PCPEIFN.
    OrthoDBi EOG72JWGX.
    PhylomeDBi Q8VI38.
    TreeFami TF330991.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 378732.
    PROi Q8VI38.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VI38.
    Genevestigatori Q8VI38.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10462. PTHR10462. 1 hit.
    Pfami PF03414. Glyco_transf_6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Forssman synthetase expression results in diminished shiga toxin susceptibility: a role for glycolipids in determining host-microbe interactions."
      Elliott S.P., Yu M., Xu H., Haslam D.B.
      Infect. Immun. 71:6543-6552(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "Preferential expression of Forssman glycolipid in undifferentiated ES cells and its chromosomal localization in mouse."
      Watanobe T., Shimizu M., Nakano M., Handa S., Yoshida M., Kushi Y.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiGBGT1_MOUSE
    AccessioniPrimary (citable) accession number: Q8VI38
    Secondary accession number(s): Q3KPA1, Q8CJF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3