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Q8VI38 (GBGT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

EC=2.4.1.88
Alternative name(s):
Forssman glycolipid synthase
Gene names
Name:Gbgt1
Synonyms:Fgs
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of Forssman glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to GalNAcb-1,3Gala-1,4Galb-1,4GlcCer (Gb4Cer). Forssman glycolipid (also called Forssman antigen; FG) probably serves for adherence of some pathogens. Conversely, it diminishes Shiga toxins susceptibility. Ref.1

Catalytic activity

UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Domain

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis By similarity.

Sequence similarities

Belongs to the glycosyltransferase 6 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
PRO_0000157296

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain28 – 347320Lumenal Potential

Amino acid modifications

Glycosylation1081N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3371S → P in AAL37034. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8VI38 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 69C7E141334A5F44

FASTA34740,502
        10         20         30         40         50         60 
MTRPRLAQGL AFFLLGGTGL WVLWKFIKDW LLVSYIPYYL PCPEFFNMKL PFRKEKPLQP 

        70         80         90        100        110        120 
VTQLQYPQPK LLEHGPTELL TLTPWLAPIV SEGTFDPELL KSMYQPLNLT IGVTVFAVGK 

       130        140        150        160        170        180 
YTCFIQRFLE SAEEFFMRGY QVHYYLFTHD PTAVPRVPLG PGRLLSIIPI QGYSRWEEIS 

       190        200        210        220        230        240 
MRRMETINKH IAKRAHKEVD YLFCVDVDMV FRNPWGPETL GDLVAAIHPG YFAVPRRKFP 

       250        260        270        280        290        300 
YERRQVSSAF VADNEGDFYY GGALFGGRVA RVYEFTRACH MAILADKANS IMAAWQEESH 

       310        320        330        340 
LNRHFIWHKP SKVLSPEYLW DERKPRPRSL KMIRFSSVKK NANWLRT 

« Hide

References

« Hide 'large scale' references
[1]"Forssman synthetase expression results in diminished shiga toxin susceptibility: a role for glycolipids in determining host-microbe interactions."
Elliott S.P., Yu M., Xu H., Haslam D.B.
Infect. Immun. 71:6543-6552(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Preferential expression of Forssman glycolipid in undifferentiated ES cells and its chromosomal localization in mouse."
Watanobe T., Shimizu M., Nakano M., Handa S., Yoshida M., Kushi Y.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF292399 mRNA. Translation: AAL37034.1.
AB084789 mRNA. Translation: BAC23060.2.
AL772249 Genomic DNA. Translation: CAM24089.1.
BC106831 mRNA. Translation: AAI06832.1.
CCDSCCDS15839.1.
RefSeqNP_631936.2. NM_139197.2.
XP_006497981.1. XM_006497918.1.
XP_006497982.1. XM_006497919.1.
XP_006497983.1. XM_006497920.1.
UniGeneMm.484270.

3D structure databases

ProteinModelPortalQ8VI38.
SMRQ8VI38. Positions 63-338.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT6. Glycosyltransferase Family 6.

Proteomic databases

PRIDEQ8VI38.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028172; ENSMUSP00000028172; ENSMUSG00000026829.
ENSMUST00000163121; ENSMUSP00000127071; ENSMUSG00000026829.
GeneID227671.
KEGGmmu:227671.
UCSCuc008iyl.2. mouse.

Organism-specific databases

CTD26301.
MGIMGI:2449143. Gbgt1.

Phylogenomic databases

eggNOGNOG43612.
GeneTreeENSGT00400000022032.
HOGENOMHOG000234339.
HOVERGENHBG003563.
InParanoidQ8VI38.
KOK00722.
OMAPCPEIFN.
OrthoDBEOG72JWGX.
PhylomeDBQ8VI38.
TreeFamTF330991.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ8VI38.
GenevestigatorQ8VI38.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR10462. PTHR10462. 1 hit.
PfamPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio378732.
PROQ8VI38.
SOURCESearch...

Entry information

Entry nameGBGT1_MOUSE
AccessionPrimary (citable) accession number: Q8VI38
Secondary accession number(s): Q3KPA1, Q8CJF3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 10, 2007
Last modified: July 9, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot