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Protein

Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

Gene

Gbgt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of Forssman glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to GalNAcb-1,3Gala-1,4Galb-1,4GlcCer (Gb4Cer). Forssman glycolipid (also called Forssman antigen; FG) probably serves for adherence of some pathogens. Conversely, it diminishes Shiga toxins susceptibility.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide.By similarity

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi206ManganeseBy similarity1
Metal bindingi208ManganeseBy similarity1
Active sitei298NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycolipid biosynthetic process Source: GO_Central
  • lipid glycosylation Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (EC:2.4.1.88By similarity)
Alternative name(s):
Forssman glycolipid synthase
Gene namesi
Name:Gbgt1
Synonyms:Fgs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2449143. Gbgt1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 347LumenalSequence analysisAdd BLAST320

GO - Cellular componenti

  • Golgi apparatus Source: GO_Central
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • vesicle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001572961 – 347Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi108N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VI38.
PRIDEiQ8VI38.

PTM databases

PhosphoSitePlusiQ8VI38.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026829.
GenevisibleiQ8VI38. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028172.

Structurei

3D structure databases

ProteinModelPortaliQ8VI38.
SMRiQ8VI38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 121Substrate bindingBy similarity6
Regioni206 – 208Substrate bindingBy similarity3
Regioni228 – 231Substrate bindingBy similarity4

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis (By similarity).By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFQJ. Eukaryota.
ENOG4111IPQ. LUCA.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8VI38.
KOiK00722.
OMAiCPEIFNM.
OrthoDBiEOG091G09TO.
PhylomeDBiQ8VI38.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VI38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPRLAQGL AFFLLGGTGL WVLWKFIKDW LLVSYIPYYL PCPEFFNMKL
60 70 80 90 100
PFRKEKPLQP VTQLQYPQPK LLEHGPTELL TLTPWLAPIV SEGTFDPELL
110 120 130 140 150
KSMYQPLNLT IGVTVFAVGK YTCFIQRFLE SAEEFFMRGY QVHYYLFTHD
160 170 180 190 200
PTAVPRVPLG PGRLLSIIPI QGYSRWEEIS MRRMETINKH IAKRAHKEVD
210 220 230 240 250
YLFCVDVDMV FRNPWGPETL GDLVAAIHPG YFAVPRRKFP YERRQVSSAF
260 270 280 290 300
VADNEGDFYY GGALFGGRVA RVYEFTRACH MAILADKANS IMAAWQEESH
310 320 330 340
LNRHFIWHKP SKVLSPEYLW DERKPRPRSL KMIRFSSVKK NANWLRT
Length:347
Mass (Da):40,502
Last modified:July 10, 2007 - v2
Checksum:i69C7E141334A5F44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337S → P in AAL37034 (PubMed:14573676).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292399 mRNA. Translation: AAL37034.1.
AB084789 mRNA. Translation: BAC23060.2.
AL772249 Genomic DNA. Translation: CAM24089.1.
BC106831 mRNA. Translation: AAI06832.1.
CCDSiCCDS15839.1.
RefSeqiNP_631936.2. NM_139197.2.
XP_006497981.1. XM_006497918.2.
UniGeneiMm.484270.

Genome annotation databases

EnsembliENSMUST00000028172; ENSMUSP00000028172; ENSMUSG00000026829.
ENSMUST00000163121; ENSMUSP00000127071; ENSMUSG00000026829.
GeneIDi227671.
KEGGimmu:227671.
UCSCiuc008iyl.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292399 mRNA. Translation: AAL37034.1.
AB084789 mRNA. Translation: BAC23060.2.
AL772249 Genomic DNA. Translation: CAM24089.1.
BC106831 mRNA. Translation: AAI06832.1.
CCDSiCCDS15839.1.
RefSeqiNP_631936.2. NM_139197.2.
XP_006497981.1. XM_006497918.2.
UniGeneiMm.484270.

3D structure databases

ProteinModelPortaliQ8VI38.
SMRiQ8VI38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028172.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

PTM databases

PhosphoSitePlusiQ8VI38.

Proteomic databases

PaxDbiQ8VI38.
PRIDEiQ8VI38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028172; ENSMUSP00000028172; ENSMUSG00000026829.
ENSMUST00000163121; ENSMUSP00000127071; ENSMUSG00000026829.
GeneIDi227671.
KEGGimmu:227671.
UCSCiuc008iyl.2. mouse.

Organism-specific databases

CTDi26301.
MGIiMGI:2449143. Gbgt1.

Phylogenomic databases

eggNOGiENOG410IFQJ. Eukaryota.
ENOG4111IPQ. LUCA.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8VI38.
KOiK00722.
OMAiCPEIFNM.
OrthoDBiEOG091G09TO.
PhylomeDBiQ8VI38.
TreeFamiTF330991.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ8VI38.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026829.
GenevisibleiQ8VI38. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGBGT1_MOUSE
AccessioniPrimary (citable) accession number: Q8VI38
Secondary accession number(s): Q3KPA1, Q8CJF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 10, 2007
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.