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Q8VI38

- GBGT1_MOUSE

UniProt

Q8VI38 - GBGT1_MOUSE

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Protein

Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

Gene

Gbgt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of Forssman glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to GalNAcb-1,3Gala-1,4Galb-1,4GlcCer (Gb4Cer). Forssman glycolipid (also called Forssman antigen; FG) probably serves for adherence of some pathogens. Conversely, it diminishes Shiga toxins susceptibility.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide.

Cofactori

Manganese.By similarity

Pathwayi

GO - Molecular functioni

  1. globoside alpha-N-acetylgalactosaminyltransferase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lipid glycosylation Source: UniProtKB
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (EC:2.4.1.88)
Alternative name(s):
Forssman glycolipid synthase
Gene namesi
Name:Gbgt1
Synonyms:Fgs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2449143. Gbgt1.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1PRO_0000157296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8VI38.

Expressioni

Gene expression databases

BgeeiQ8VI38.
GenevestigatoriQ8VI38.

Structurei

3D structure databases

ProteinModelPortaliQ8VI38.
SMRiQ8VI38. Positions 63-338.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Topological domaini28 – 347320LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis (By similarity).By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43612.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ8VI38.
KOiK00722.
OMAiPCPEIFN.
OrthoDBiEOG72JWGX.
PhylomeDBiQ8VI38.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VI38-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRPRLAQGL AFFLLGGTGL WVLWKFIKDW LLVSYIPYYL PCPEFFNMKL
60 70 80 90 100
PFRKEKPLQP VTQLQYPQPK LLEHGPTELL TLTPWLAPIV SEGTFDPELL
110 120 130 140 150
KSMYQPLNLT IGVTVFAVGK YTCFIQRFLE SAEEFFMRGY QVHYYLFTHD
160 170 180 190 200
PTAVPRVPLG PGRLLSIIPI QGYSRWEEIS MRRMETINKH IAKRAHKEVD
210 220 230 240 250
YLFCVDVDMV FRNPWGPETL GDLVAAIHPG YFAVPRRKFP YERRQVSSAF
260 270 280 290 300
VADNEGDFYY GGALFGGRVA RVYEFTRACH MAILADKANS IMAAWQEESH
310 320 330 340
LNRHFIWHKP SKVLSPEYLW DERKPRPRSL KMIRFSSVKK NANWLRT
Length:347
Mass (Da):40,502
Last modified:July 10, 2007 - v2
Checksum:i69C7E141334A5F44
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371S → P in AAL37034. (PubMed:14573676)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF292399 mRNA. Translation: AAL37034.1.
AB084789 mRNA. Translation: BAC23060.2.
AL772249 Genomic DNA. Translation: CAM24089.1.
BC106831 mRNA. Translation: AAI06832.1.
CCDSiCCDS15839.1.
RefSeqiNP_631936.2. NM_139197.2.
XP_006497981.1. XM_006497918.1.
XP_006497982.1. XM_006497919.1.
XP_006497983.1. XM_006497920.1.
UniGeneiMm.484270.

Genome annotation databases

EnsembliENSMUST00000028172; ENSMUSP00000028172; ENSMUSG00000026829.
ENSMUST00000163121; ENSMUSP00000127071; ENSMUSG00000026829.
GeneIDi227671.
KEGGimmu:227671.
UCSCiuc008iyl.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF292399 mRNA. Translation: AAL37034.1 .
AB084789 mRNA. Translation: BAC23060.2 .
AL772249 Genomic DNA. Translation: CAM24089.1 .
BC106831 mRNA. Translation: AAI06832.1 .
CCDSi CCDS15839.1.
RefSeqi NP_631936.2. NM_139197.2.
XP_006497981.1. XM_006497918.1.
XP_006497982.1. XM_006497919.1.
XP_006497983.1. XM_006497920.1.
UniGenei Mm.484270.

3D structure databases

ProteinModelPortali Q8VI38.
SMRi Q8VI38. Positions 63-338.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT6. Glycosyltransferase Family 6.

Proteomic databases

PRIDEi Q8VI38.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028172 ; ENSMUSP00000028172 ; ENSMUSG00000026829 .
ENSMUST00000163121 ; ENSMUSP00000127071 ; ENSMUSG00000026829 .
GeneIDi 227671.
KEGGi mmu:227671.
UCSCi uc008iyl.2. mouse.

Organism-specific databases

CTDi 26301.
MGIi MGI:2449143. Gbgt1.

Phylogenomic databases

eggNOGi NOG43612.
GeneTreei ENSGT00400000022032.
HOGENOMi HOG000234339.
HOVERGENi HBG003563.
InParanoidi Q8VI38.
KOi K00722.
OMAi PCPEIFN.
OrthoDBi EOG72JWGX.
PhylomeDBi Q8VI38.
TreeFami TF330991.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 378732.
PROi Q8VI38.
SOURCEi Search...

Gene expression databases

Bgeei Q8VI38.
Genevestigatori Q8VI38.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10462. PTHR10462. 1 hit.
Pfami PF03414. Glyco_transf_6. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Forssman synthetase expression results in diminished shiga toxin susceptibility: a role for glycolipids in determining host-microbe interactions."
    Elliott S.P., Yu M., Xu H., Haslam D.B.
    Infect. Immun. 71:6543-6552(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "Preferential expression of Forssman glycolipid in undifferentiated ES cells and its chromosomal localization in mouse."
    Watanobe T., Shimizu M., Nakano M., Handa S., Yoshida M., Kushi Y.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiGBGT1_MOUSE
AccessioniPrimary (citable) accession number: Q8VI38
Secondary accession number(s): Q3KPA1, Q8CJF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 10, 2007
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3