Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Paxillin

Gene

Pxn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).By similarity

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • BH4 domain binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • neuropilin binding Source: BHF-UCL
  • receptor signaling complex scaffold activity Source: MGI
  • vinculin binding Source: MGI

GO - Biological processi

  • activation of MAPK activity Source: MGI
  • branching morphogenesis of an epithelial tube Source: MGI
  • cytoskeleton organization Source: MGI
  • endothelial cell migration Source: MGI
  • focal adhesion assembly Source: MGI
  • growth hormone receptor signaling pathway Source: MGI
  • integrin-mediated signaling pathway Source: MGI
  • lamellipodium assembly Source: MGI
  • peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of angiogenesis Source: BHF-UCL
  • positive regulation of protein kinase activity Source: MGI
  • positive regulation of stress fiber assembly Source: MGI
  • regulation of cell shape Source: MGI
  • substrate adhesion-dependent cell spreading Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: UniProtKB

Keywordsi

Biological processCell adhesion
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-180292 GAB1 signalosome
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-445355 Smooth Muscle Contraction
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Names & Taxonomyi

Protein namesi
Recommended name:
Paxillin
Gene namesi
Name:Pxn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:108295 Pxn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000758541 – 591PaxillinAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei31Phosphotyrosine; by PTK6By similarity1
Modified residuei83PhosphoserineCombined sources1
Modified residuei88PhosphotyrosineCombined sources1
Modified residuei106PhosphoserineBy similarity1
Modified residuei118Phosphotyrosine; by PTK6Combined sources1 Publication1
Modified residuei119PhosphoserineBy similarity1
Modified residuei126PhosphoserineCombined sources1
Modified residuei130PhosphoserineCombined sources1
Modified residuei132PhosphothreonineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei143PhosphoserineCombined sources1
Modified residuei181PhosphotyrosineBy similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei244Phosphoserine; by CDK5By similarity1
Modified residuei250PhosphoserineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei272PhosphoserineCombined sources1
Modified residuei303PhosphoserineBy similarity1
Modified residuei322PhosphoserineCombined sources1
Modified residuei332PhosphoserineBy similarity1
Modified residuei340PhosphoserineCombined sources1
Modified residuei533PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation (By similarity). Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion (By similarity). Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8VI36
PeptideAtlasiQ8VI36
PRIDEiQ8VI36

PTM databases

iPTMnetiQ8VI36
PhosphoSitePlusiQ8VI36
SwissPalmiQ8VI36

Miscellaneous databases

PMAP-CutDBiQ8VI36

Expressioni

Gene expression databases

BgeeiENSMUSG00000029528
CleanExiMM_PXN
ExpressionAtlasiQ8VI36 baseline and differential
GenevisibleiQ8VI36 MM

Interactioni

Subunit structurei

Interacts with VCL and SRC (via SH3 domain). Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2. Interacts with RNF5 and PDCD10 (By similarity). Interacts with SORBS1, PARVA and PARVB. Interacts with NEK3 and this interaction is prolactin-dependent (By similarity). Interacts with PTK2/FAK1. Interacts with PTK6 (By similarity). Interacts with PTK2B/PYK2. Interacts with CD36. Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • BH4 domain binding Source: MGI
  • neuropilin binding Source: BHF-UCL
  • receptor signaling complex scaffold activity Source: MGI
  • vinculin binding Source: MGI

Protein-protein interaction databases

BioGridi202525, 18 interactors
IntActiQ8VI36, 20 interactors
MINTiQ8VI36
STRINGi10090.ENSMUSP00000083709

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 9Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W93X-ray2.00D/E/F1-20[»]
ProteinModelPortaliQ8VI36
SMRiQ8VI36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini356 – 415LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST60
Domaini416 – 473LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST58
Domaini474 – 533LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST60
Domaini534 – 591LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 15LD motif 1Add BLAST13
Motifi144 – 156LD motif 2Add BLAST13
Motifi216 – 228LD motif 3Add BLAST13
Motifi265 – 276LD motif 4Add BLAST12
Motifi333 – 345LD motif 5Add BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 53Pro-rich8

Sequence similaritiesi

Belongs to the paxillin family.Curated

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703 Eukaryota
ENOG410XRD4 LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000018764
HOVERGENiHBG001512
InParanoidiQ8VI36
KOiK05760
PhylomeDBiQ8VI36

Family and domain databases

InterProiView protein in InterPro
IPR001904 Paxillin
IPR017305 Tgfb1i1/Leupaxin/TGFB1I1
IPR001781 Znf_LIM
PANTHERiPTHR24216:SF11 PTHR24216:SF11, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
PIRSFiPIRSF037881 Leupaxin, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: Q8VI36-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDLDALLAD LESTTSHISK RPVFLSEEPP YSYPTGNHTY QEIAVPPPVP
60 70 80 90 100
PPPSSEALNG TVLDPLDQWQ PSGSRYAHQQ PPSPLPVYSS SAKNSSASNT
110 120 130 140 150
QDGVGSLCSR AGEEEHVYSF PNKQKSAEPS PTVMSSSLGS NLSELDRLLL
160 170 180 190 200
ELNAVQHSPP GFPADEAESS PPLPGALSPL YGIPENNTPL GGKAGPLVKE
210 220 230 240 250
KPKRNGGRGL EDVRPSVESL LDELESSVPS PVPAITVNQG EMSSPQRVTS
260 270 280 290 300
SQQQTRISAS SATRELDELM ASLSDFKMQG LEQRVDGERP WAAGWPPSSR
310 320 330 340 350
QSSPEGQDEG GFMAQGKTGS SSPPGGLSKP GSQLDSMLGS LQSDLNKLGV
360 370 380 390 400
ATVAKGVCGA CKKPIAGQVV TAMGKTWHPE HFVCTHCQEE IGSRNFFERD
410 420 430 440 450
GQPYCEKDYH SLFSPRCYYC NGPILDKVVT ALDRTWHPEH FFCAQCGAFF
460 470 480 490 500
GPEGFHEKDG KAYCRKDYFD MFAPKCGGCA RAILENYISA LNTLWHPECF
510 520 530 540 550
VCRECFTPFV NGSFFEHDGQ PYCEVHYHER RGSLCSGCQK PITGRCITAM
560 570 580 590
AKKFHPEHFV CAFCLKQLNK GTFKEQNDKP YCQSCFVKLF C
Length:591
Mass (Da):64,476
Last modified:March 1, 2002 - v1
Checksum:iB41A1892E6F60544
GO
Isoform Alpha (identifier: Q8VI36-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-311: Missing.

Show »
Length:557
Mass (Da):60,811
Checksum:i483D880CF16289B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71P → R in BAE42452 (PubMed:16141072).Curated1
Sequence conflicti212D → N in BAE34151 (PubMed:16141072).Curated1
Sequence conflicti294G → S in BAE34151 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016357278 – 311Missing in isoform Alpha. 2 PublicationsAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF293883 mRNA Translation: AAL71910.1
AF293882 mRNA Translation: AAL71909.1
AK149933 mRNA Translation: BAE29176.1
AK157688 mRNA Translation: BAE34151.1
AK167299 mRNA Translation: BAE39404.1
AK171436 mRNA Translation: BAE42452.1
CCDSiCCDS19593.1 [Q8VI36-2]
CCDS39229.1 [Q8VI36-1]
RefSeqiNP_035353.1, NM_011223.3 [Q8VI36-2]
NP_598676.2, NM_133915.3
UniGeneiMm.18714

Genome annotation databases

EnsembliENSMUST00000067268; ENSMUSP00000069624; ENSMUSG00000029528 [Q8VI36-2]
GeneIDi19303
KEGGimmu:19303
UCSCiuc008zeb.2 mouse [Q8VI36-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPAXI_MOUSE
AccessioniPrimary (citable) accession number: Q8VI36
Secondary accession number(s): Q3TB62, Q3TZQ6, Q8VI37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health