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Protein

Transcription initiation factor TFIID subunit 9

Gene

Taf9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 9
Alternative name(s):
RNA polymerase II TBP-associated factor subunit G
Transcription initiation factor TFIID 31 kDa subunit
Short name:
TAFII-31
Short name:
TAFII31
Transcription initiation factor TFIID 32 kDa subunit
Short name:
TAFII-32
Short name:
TAFII32
Gene namesi
Name:Taf9
Synonyms:Taf2g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1888697. Taf9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Transcription initiation factor TFIID subunit 9PRO_0000118889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51N6-acetyllysineBy similarity
Modified residuei149 – 1491PhosphoserineBy similarity
Modified residuei152 – 1521PhosphoserineBy similarity
Modified residuei155 – 1551PhosphoserineCombined sources
Modified residuei158 – 1581PhosphoserineBy similarity
Modified residuei159 – 1591PhosphothreonineCombined sources
Modified residuei161 – 1611PhosphothreonineCombined sources
Modified residuei164 – 1641PhosphothreonineCombined sources
Modified residuei178 – 1781PhosphothreonineBy similarity
Modified residuei181 – 1811PhosphoserineBy similarity
Modified residuei196 – 1961PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VI33.
MaxQBiQ8VI33.
PaxDbiQ8VI33.
PeptideAtlasiQ8VI33.
PRIDEiQ8VI33.

PTM databases

iPTMnetiQ8VI33.
PhosphoSiteiQ8VI33.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052293.
ExpressionAtlasiQ8VI33. baseline and differential.
GenevisibleiQ8VI33. MM.

Interactioni

Subunit structurei

Component of TFIID, the TATA-binding protein-free TAF complex (TFTC), the PCAF complex and the STAGA transcription coactivator-HAT complex. The PCAF complex consists at least of TADA2L/ADA2, SUPT3H/SPT3, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. The STAGA transcription coactivator-HAT complex consists at least of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Binds N-terminal domain of p53/TP53 which is essential for transcription. Binds TFIIB and the Herpes simplex virus activator VP16. Forms a heterodimer with TAF6/TAFII80 in a complex with the TAF4B/TAFII105-TAF12/TAFII20 heterodimer. Also interacts with TAF5. Binds directly DNA. Increased DNA binding when complexed with TAF6/TAFII80 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi223860. 2 interactions.
DIPiDIP-48737N.
IntActiQ8VI33. 4 interactions.
MINTiMINT-4609777.
STRINGi10090.ENSMUSP00000081772.

Structurei

3D structure databases

ProteinModelPortaliQ8VI33.
SMRiQ8VI33. Positions 13-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi248 – 26215Poly-AspAdd
BLAST

Sequence similaritiesi

Belongs to the TAF9 family.Curated

Phylogenomic databases

eggNOGiKOG3334. Eukaryota.
COG5094. LUCA.
GeneTreeiENSGT00390000001626.
HOGENOMiHOG000231730.
HOVERGENiHBG002304.
InParanoidiQ8VI33.
KOiK14535.
OrthoDBiEOG091G0RPE.
PhylomeDBiQ8VI33.
TreeFamiTF351417.

Family and domain databases

CDDicd07979. TAF9. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003162. TFIID-31.
[Graphical view]
PfamiPF02291. TFIID-31kDa. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VI33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGKMASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI
60 70 80 90 100
LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPRD FLLDIARQRN
110 120 130 140 150
QTPLPLIKPY SGPRLPPDRY CLTAPNYRLK SLQKKAPAPA GRITVPRLSV
160 170 180 190 200
GSVSSRPSTP TLGTPTPQTM SVSTKVGTPM SLTGQRFTVQ MPASQSPAVK
210 220 230 240 250
ASIPATSTVQ NVLINPSLIG SKNILITTNM VSQNTAESAN ALKRKREDDD
260
DDDDDDDDDD YDNM
Length:264
Mass (Da):28,979
Last modified:March 1, 2002 - v1
Checksum:iEFB2AE27AEFEE794
GO

Sequence cautioni

The sequence BAB26216 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611T → P in AAH43028 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305839 mRNA. Translation: AAL47693.1.
AK009322 mRNA. Translation: BAB26216.2. Different initiation.
BC042723 mRNA. Translation: AAH42723.1.
BC043028 mRNA. Translation: AAH43028.1.
BC108348 mRNA. Translation: AAI08349.1.
CCDSiCCDS26735.1.
RefSeqiNP_001015889.1. NM_001015889.2.
NP_081415.1. NM_027139.5.
UniGeneiMm.301148.

Genome annotation databases

EnsembliENSMUST00000084721; ENSMUSP00000081772; ENSMUSG00000078941.
ENSMUST00000167256; ENSMUSP00000132143; ENSMUSG00000052293.
ENSMUST00000190594; ENSMUSP00000140244; ENSMUSG00000052293.
GeneIDi108143.
KEGGimmu:108143.
UCSCiuc007rrf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305839 mRNA. Translation: AAL47693.1.
AK009322 mRNA. Translation: BAB26216.2. Different initiation.
BC042723 mRNA. Translation: AAH42723.1.
BC043028 mRNA. Translation: AAH43028.1.
BC108348 mRNA. Translation: AAI08349.1.
CCDSiCCDS26735.1.
RefSeqiNP_001015889.1. NM_001015889.2.
NP_081415.1. NM_027139.5.
UniGeneiMm.301148.

3D structure databases

ProteinModelPortaliQ8VI33.
SMRiQ8VI33. Positions 13-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223860. 2 interactions.
DIPiDIP-48737N.
IntActiQ8VI33. 4 interactions.
MINTiMINT-4609777.
STRINGi10090.ENSMUSP00000081772.

PTM databases

iPTMnetiQ8VI33.
PhosphoSiteiQ8VI33.

Proteomic databases

EPDiQ8VI33.
MaxQBiQ8VI33.
PaxDbiQ8VI33.
PeptideAtlasiQ8VI33.
PRIDEiQ8VI33.

Protocols and materials databases

DNASUi108143.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084721; ENSMUSP00000081772; ENSMUSG00000078941.
ENSMUST00000167256; ENSMUSP00000132143; ENSMUSG00000052293.
ENSMUST00000190594; ENSMUSP00000140244; ENSMUSG00000052293.
GeneIDi108143.
KEGGimmu:108143.
UCSCiuc007rrf.2. mouse.

Organism-specific databases

CTDi6880.
MGIiMGI:1888697. Taf9.

Phylogenomic databases

eggNOGiKOG3334. Eukaryota.
COG5094. LUCA.
GeneTreeiENSGT00390000001626.
HOGENOMiHOG000231730.
HOVERGENiHBG002304.
InParanoidiQ8VI33.
KOiK14535.
OrthoDBiEOG091G0RPE.
PhylomeDBiQ8VI33.
TreeFamiTF351417.

Enzyme and pathway databases

ReactomeiR-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

PROiQ8VI33.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052293.
ExpressionAtlasiQ8VI33. baseline and differential.
GenevisibleiQ8VI33. MM.

Family and domain databases

CDDicd07979. TAF9. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003162. TFIID-31.
[Graphical view]
PfamiPF02291. TFIID-31kDa. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF9_MOUSE
AccessioniPrimary (citable) accession number: Q8VI33
Secondary accession number(s): Q32P09, Q80XS3, Q9CV61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

AK6 and TAF9 were initially considered as products of the same gene since they share two exons. However, they are translated from different initiation codons and reading frames and encode unrelated proteins. This arrangement is conserved in some mammalian species.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.