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Q8VI24

- SATB2_MOUSE

UniProt

Q8VI24 - SATB2_MOUSE

Protein

DNA-binding protein SATB2

Gene

Satb2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating Bcl11b expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi350 – 43788CUT 1PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi473 – 56088CUT 2PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi615 – 67460HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: MGI
    4. sequence-specific DNA binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. cartilage development Source: MGI
    2. cellular response to organic substance Source: MGI
    3. chromatin remodeling Source: MGI
    4. commitment of neuronal cell to specific neuron type in forebrain Source: MGI
    5. embryonic pattern specification Source: MGI
    6. embryonic skeletal system morphogenesis Source: MGI
    7. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    8. neuron migration Source: MGI
    9. osteoblast development Source: MGI
    10. palate development Source: MGI
    11. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    12. regulation of gene expression Source: MGI
    13. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein SATB2
    Alternative name(s):
    Special AT-rich sequence-binding protein 2
    Gene namesi
    Name:Satb2
    Synonyms:Kiaa1034
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2679336. Satb2.

    Subcellular locationi

    Nucleus matrix PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. histone deacetylase complex Source: MGI
    3. nuclear chromatin Source: MGI
    4. nuclear matrix Source: UniProtKB-SubCell
    5. nucleus Source: MGI
    6. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Malformations in 2 of the major axonal tracts interconnecting the cortical hemispheres: the corpus callosum (c.c) and the anterior commissure (a.c). Misrouted afferent and efferent cortical axon connections. Impaired migration of upper-layer neurons. Ectopic expression of Ctip2. Craniofacial abnormalities and defects in osteoblast differentiation and function.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 733733DNA-binding protein SATB2PRO_0000202401Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki233 – 233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Cross-linki350 – 350Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

    Post-translational modificationi

    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiQ8VI24.
    PaxDbiQ8VI24.
    PRIDEiQ8VI24.

    PTM databases

    PhosphoSiteiQ8VI24.

    Expressioni

    Tissue specificityi

    Expressed in cortical neurons that extend axons across the corpus callosum. Also expressed in branchial arches and in cells of the osteoblast lineage, but not in chondrocytes and osteoclasts.2 Publications

    Developmental stagei

    Expression first detected at 10.5 dpc in the maxillary component of the first pharyngeal arch and the lateral aspect of the frontonasal process in the regions that will subsequently fuse to form the primary palate. At 11 - 11.5 dpc, the expression pattern demarcates the region of the medial aspect of the maxillary process within the primitive oral cavity, which will form the palate shelf. By 12.5 dpc, symmetrical expression is seen in the medial edges of the developing palate shelves and this continues until 13.5 dpc when the strongest expression is in the mesenchyme underlying the medial edge epithelia. By the time of palatal shelf fusion at 14.5 dpc the expression is dramatically down-regulated. No expression detected elsewhere in the embryo at any stage examined.1 Publication

    Gene expression databases

    ArrayExpressiQ8VI24.
    BgeeiQ8VI24.
    CleanExiMM_SATB2.
    GenevestigatoriQ8VI24.

    Interactioni

    Subunit structurei

    Interacts with PIAS1 By similarity. Interacts with ATF4 and RUNX2; resulting in enhanced DNA binding and transactivation by these transcription factors.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi229351. 6 interactions.
    DIPiDIP-60021N.
    IntActiQ8VI24. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VI24.
    SMRiQ8VI24. Positions 57-157, 165-231, 350-437, 473-560, 615-674.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CUT homeobox family.Curated
    Contains 2 CUT DNA-binding domains.PROSITE-ProRule annotation
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox, Repeat

    Phylogenomic databases

    eggNOGiNOG313826.
    GeneTreeiENSGT00390000008096.
    HOGENOMiHOG000004805.
    HOVERGENiHBG054240.
    InParanoidiQ8VI24.
    OMAiPPAEDSC.
    OrthoDBiEOG7FBRH5.
    PhylomeDBiQ8VI24.
    TreeFamiTF332714.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 2 hits.
    InterProiIPR003350. Hmoeo_CUT.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    [Graphical view]
    PfamiPF02376. CUT. 2 hits.
    PF00046. Homeobox. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 2 hits.
    PROSITEiPS51042. CUT. 2 hits.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VI24-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERRSESPCL RDSPDRRSGS PDVKGPPPVK VARLEQNGSP MGARGRPNGA    50
    VAKAVGGLMI PVFCVVEQLD GSLEYDNREE HAEFVLVRKD VLFSQLVETA 100
    LLALGYSHSS AAQAQGIIKL GRWNPLPLSY VTDAPDATVA DMLQDVYHVV 150
    TLKIQLQSCS KLEDLPAEQW NHATVRNALK ELLKEMNQST LAKECPLSQS 200
    MISSIVNSTY YANVSATKCQ EFGRWYKKYK KIKVERVERE NLSDYCVLGQ 250
    RPMHLPNMNQ LASLGKTNEQ SPHSQIHHST PIRNQVPALQ PIMSPGLLSP 300
    QLSPQLVRQQ IAMAHLINQQ IAVSRLLAHQ HPQAINQQFL NHPPIPRAVK 350
    PEPTNSSVEV SPDIYQQVRD ELKRASVSQA VFARVAFNRT QGLLSEILRK 400
    EEDPRTASQS LLVNLRAMQN FLNLPEVERD RIYQDERERS MNPNVSMVSS 450
    ASSSPSSSRT PQAKTSTPTT DLPIKVDGAN VNITAAIYDE IQQEMKRAKV 500
    SQALFAKVAA NKSQGWLCEL LRWKENPSPE NRTLWENLCT IRRFLNLPQH 550
    ERDVIYEEES RHHHSERMQH VVQLPPEPVQ VLHRQQSQPT KESSPPREEA 600
    PPPPPPTEDS CAKKPRSRTK ISLEALGILQ SFIHDVGLYP DQEAIHTLSA 650
    QLDLPKHTII KFFQNQRYHV KHHGKLKEHL GSAVDVAEYK DEELLTESEE 700
    NDSEEGSEEM YKVEAEEENA DKSKAAPAET DQR 733
    Length:733
    Mass (Da):82,559
    Last modified:March 1, 2002 - v1
    Checksum:i153CFD1CC3491F25
    GO
    Isoform 2 (identifier: Q8VI24-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         58-116: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:674
    Mass (Da):76,039
    Checksum:i199D04A796B2FB77
    GO

    Sequence cautioni

    The sequence BAC98080.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei58 – 11659Missing in isoform 2. 1 PublicationVSP_008967Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF319623 mRNA. Translation: AAL37172.1.
    AK129270 mRNA. Translation: BAC98080.1. Different initiation.
    CCDSiCCDS14965.1. [Q8VI24-1]
    RefSeqiNP_631885.1. NM_139146.2. [Q8VI24-1]
    UniGeneiMm.145599.

    Genome annotation databases

    EnsembliENSMUST00000042857; ENSMUSP00000046067; ENSMUSG00000038331. [Q8VI24-2]
    ENSMUST00000114415; ENSMUSP00000110057; ENSMUSG00000038331. [Q8VI24-1]
    GeneIDi212712.
    KEGGimmu:212712.
    UCSCiuc007bas.1. mouse. [Q8VI24-1]
    uc011wlc.1. mouse. [Q8VI24-2]

    Keywords - Coding sequence diversityi

    Alternative splicing, Chromosomal rearrangement

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF319623 mRNA. Translation: AAL37172.1 .
    AK129270 mRNA. Translation: BAC98080.1 . Different initiation.
    CCDSi CCDS14965.1. [Q8VI24-1 ]
    RefSeqi NP_631885.1. NM_139146.2. [Q8VI24-1 ]
    UniGenei Mm.145599.

    3D structure databases

    ProteinModelPortali Q8VI24.
    SMRi Q8VI24. Positions 57-157, 165-231, 350-437, 473-560, 615-674.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229351. 6 interactions.
    DIPi DIP-60021N.
    IntActi Q8VI24. 1 interaction.

    PTM databases

    PhosphoSitei Q8VI24.

    Proteomic databases

    MaxQBi Q8VI24.
    PaxDbi Q8VI24.
    PRIDEi Q8VI24.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042857 ; ENSMUSP00000046067 ; ENSMUSG00000038331 . [Q8VI24-2 ]
    ENSMUST00000114415 ; ENSMUSP00000110057 ; ENSMUSG00000038331 . [Q8VI24-1 ]
    GeneIDi 212712.
    KEGGi mmu:212712.
    UCSCi uc007bas.1. mouse. [Q8VI24-1 ]
    uc011wlc.1. mouse. [Q8VI24-2 ]

    Organism-specific databases

    CTDi 23314.
    MGIi MGI:2679336. Satb2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG313826.
    GeneTreei ENSGT00390000008096.
    HOGENOMi HOG000004805.
    HOVERGENi HBG054240.
    InParanoidi Q8VI24.
    OMAi PPAEDSC.
    OrthoDBi EOG7FBRH5.
    PhylomeDBi Q8VI24.
    TreeFami TF332714.

    Miscellaneous databases

    NextBioi 373676.
    PROi Q8VI24.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VI24.
    Bgeei Q8VI24.
    CleanExi MM_SATB2.
    Genevestigatori Q8VI24.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 2 hits.
    InterProi IPR003350. Hmoeo_CUT.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    [Graphical view ]
    Pfami PF02376. CUT. 2 hits.
    PF00046. Homeobox. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 2 hits.
    PROSITEi PS51042. CUT. 2 hits.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryonic tail.
    3. "SATB2 is a multifunctional determinant of craniofacial patterning and osteoblast differentiation."
      Dobreva G., Chahrour M., Dautzenberg M., Chirivella L., Kanzler B., Farinas I., Karsenty G., Grosschedl R.
      Cell 125:971-986(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH ATF4 AND RUNX2, TISSUE SPECIFICITY.
    4. "Satb2 regulates callosal projection neuron identity in the developing cerebral cortex."
      Alcamo E.A., Chirivella L., Dautzenberg M., Dobreva G., Farinas I., Grosschedl R., McConnell S.K.
      Neuron 57:364-377(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    5. "Satb2 is a postmitotic determinant for upper-layer neuron specification in the neocortex."
      Britanova O., de Juan Romero C., Cheung A., Kwan K.Y., Schwark M., Gyorgy A., Vogel T., Akopov S., Mitkovski M., Agoston D., Sestan N., Molnar Z., Tarabykin V.
      Neuron 57:378-392(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiSATB2_MOUSE
    AccessioniPrimary (citable) accession number: Q8VI24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2003
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3