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Q8VI23 (S12A8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 12 member 8
Alternative name(s):
Cation-chloride cotransporter 9
Gene names
Name:Slc12a8
Synonyms:Ccc9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cation/chloride cotransporter that may play a role in the control of keratinocyte proliferation By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the SLC12A transporter family.

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Symport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandChloride
Potassium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpotassium ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionsymporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VI23-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VI23-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MAQRSP → MSQV
Isoform 3 (identifier: Q8VI23-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.
     304-352: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8VI23-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-246: Missing.
     304-352: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 705705Solute carrier family 12 member 8
PRO_0000305288

Regions

Transmembrane38 – 5821Helical; Potential
Transmembrane69 – 8921Helical; Potential
Transmembrane92 – 11221Helical; Potential
Transmembrane121 – 14121Helical; Potential
Transmembrane159 – 17921Helical; Potential
Transmembrane181 – 20121Helical; Potential
Transmembrane232 – 25221Helical; Potential
Transmembrane268 – 28821Helical; Potential
Transmembrane306 – 32621Helical; Potential
Transmembrane368 – 38821Helical; Potential
Transmembrane390 – 41021Helical; Potential
Transmembrane587 – 60721Helical; Potential
Transmembrane612 – 63221Helical; Potential

Natural variations

Alternative sequence1 – 246246Missing in isoform 4.
VSP_028333
Alternative sequence1 – 3131Missing in isoform 3.
VSP_028334
Alternative sequence1 – 66MAQRSP → MSQV in isoform 2.
VSP_028335
Alternative sequence304 – 35249Missing in isoform 3 and isoform 4.
VSP_028336

Experimental info

Sequence conflict1831L → P in AAL37178. Ref.1
Sequence conflict2361F → L in AAL37178. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 0AF9193CB067D315

FASTA70577,020
        10         20         30         40         50         60 
MAQRSPQELF HEAAQQGILA QPQPWWKIQL FMWEPVLFGT WDGVFTSCMI NIFGVVLFLR 

        70         80         90        100        110        120 
TGWLVGNTGV LLGLLLVSFV VLVALITVLS GIGVAEHGGI SSGGVYSMIS SVLGGQMGGT 

       130        140        150        160        170        180 
VGLLYVFGQC VAGAMYITGF AESISDLLGL GDIWAVRGIS VAVLLALLGI NLAGVKWIIR 

       190        200        210        220        230        240 
LQLLLLLLLA VSTLDFVVGS FTHLDPEHGF IGYSPELLQS NILPEYSPGE SFFTVFGVFF 

       250        260        270        280        290        300 
PAATGVMAGF NMGGDLRDPA DSVPLGSLAA VGVSWFLYII FAFLLGAVCT REALRSDFLI 

       310        320        330        340        350        360 
AEKVSLVGFL FLLGLYISSL ASCMGGLYGA PRILQCIAQD KVIPALAFLA NGKGPNKTPV 

       370        380        390        400        410        420 
AAICLTSLVT MAFVLVGQVN VLAPVVTINF MLTYIMVDYS YFALSMAHCG LAPSPEPVPR 

       430        440        450        460        470        480 
QGPDTLHCSE HLLQDRAPSY GSDVPARSLS EGTLLEFTKD MDQFLQPIEE LESRQLGSRE 

       490        500        510        520        530        540 
GNNPKNQKRK GKKGAKQTLQ DSFLLDPGSP LSFPTRTSER LSVAFCGEQE SYQKQQTSRS 

       550        560        570        580        590        600 
ESHDHLVPDL RNQPRVNRED FFLKCRLQEQ EIQRRPSVFY ACMCNPWVSL LGALASLLIM 

       610        620        630        640        650        660 
FVIQWLYTLA SMGVAALVYF YIGQASPGLY LGSASNFSFF QWMKSFLVPS CRSLRSAQEQ 

       670        680        690        700 
IILAPSPAKV DMAMTQLTQD NADFATRDRY HHSSFLSREQ LMPPY 

« Hide

Isoform 2 [UniParc].

Checksum: 90FCDF8BF7ADF11E
Show »

FASTA70376,794
Isoform 3 [UniParc].

Checksum: D0C2AED2F5DEBE7B
Show »

FASTA62568,291
Isoform 4 [UniParc].

Checksum: CF8D2962EAAC808F
Show »

FASTA41045,717

References

« Hide 'large scale' references
[1]"Cloning and characterization of mouse CCC9 (SLC12A9), a new member of the cation-chloride cotransporter gene family."
Mount D.B.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Strain: C57BL/6J.
Tissue: Ovary.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: FVB/N.
Tissue: Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF319951 mRNA. Translation: AAL37178.1.
AK165080 mRNA. Translation: BAE38029.1.
AC121570 Genomic DNA. No translation available.
AC127412 Genomic DNA. No translation available.
BC030926 mRNA. Translation: AAH30926.1.
RefSeqNP_001077371.1. NM_001083902.1.
NP_599012.2. NM_134251.2.
XP_006521883.1. XM_006521820.1.
UniGeneMm.35253.

3D structure databases

ProteinModelPortalQ8VI23.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8VI23.

Proteomic databases

PRIDEQ8VI23.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000059056; ENSMUSP00000062337; ENSMUSG00000035506. [Q8VI23-1]
ENSMUST00000119173; ENSMUSP00000113633; ENSMUSG00000035506. [Q8VI23-3]
ENSMUST00000121925; ENSMUSP00000112439; ENSMUSG00000035506. [Q8VI23-1]
GeneID171286.
KEGGmmu:171286.
UCSCuc007zad.1. mouse. [Q8VI23-1]
uc007zae.1. mouse. [Q8VI23-3]
uc007zag.1. mouse. [Q8VI23-4]

Organism-specific databases

CTD84561.
MGIMGI:2443672. Slc12a8.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00560000076892.
HOGENOMHOG000045198.
HOVERGENHBG108430.
InParanoidQ8VI23.
KOK14428.
OMAGRGPNKV.
OrthoDBEOG7VB2DV.
PhylomeDBQ8VI23.
TreeFamTF313191.

Gene expression databases

ArrayExpressQ8VI23.
BgeeQ8VI23.
GenevestigatorQ8VI23.

Family and domain databases

InterProIPR004841. AA-permease/SLC12A_dom.
[Graphical view]
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio370967.
PROQ8VI23.
SOURCESearch...

Entry information

Entry nameS12A8_MOUSE
AccessionPrimary (citable) accession number: Q8VI23
Secondary accession number(s): Q3TNQ9, Q8K0M9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot