Q8VI23 (S12A8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 12 member 8

Alternative name(s):
Cation-chloride cotransporter 9

Gene names

Name:	Slc12a8
Synonyms:	Ccc9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cation/chloride cotransporter that may play a role in the control of keratinocyte proliferation By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SLC12A transporter family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Chloride Potassium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VI23-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VI23-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MAQRSP → MSQV

Isoform 3 (identifier: Q8VI23-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.
     304-352: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8VI23-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-246: Missing.
     304-352: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Solute carrier family 12 member 8		PRO_0000305288
Regions
	Transmembrane	38 – 58	21	Helical; Potential
	Transmembrane	69 – 89	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Transmembrane	232 – 252	21	Helical; Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Transmembrane	368 – 388	21	Helical; Potential
	Transmembrane	390 – 410	21	Helical; Potential
	Transmembrane	587 – 607	21	Helical; Potential
	Transmembrane	612 – 632	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 246	246	Missing in isoform 4.		VSP_028333
	Alternative sequence	1 – 31	31	Missing in isoform 3.		VSP_028334
	Alternative sequence	1 – 6	6	MAQRSP → MSQV in isoform 2.		VSP_028335
	Alternative sequence	304 – 352	49	Missing in isoform 3 and isoform 4.		VSP_028336
Experimental info
	Sequence conflict	183	1	L → P in AAL37178. Ref.1
	Sequence conflict	236	1	F → L in AAL37178. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: 0AF9193CB067D315	FASTA	705	77,020
	10 20 30 40 50 60 MAQRSPQELF HEAAQQGILA QPQPWWKIQL FMWEPVLFGT WDGVFTSCMI NIFGVVLFLR 70 80 90 100 110 120 TGWLVGNTGV LLGLLLVSFV VLVALITVLS GIGVAEHGGI SSGGVYSMIS SVLGGQMGGT 130 140 150 160 170 180 VGLLYVFGQC VAGAMYITGF AESISDLLGL GDIWAVRGIS VAVLLALLGI NLAGVKWIIR 190 200 210 220 230 240 LQLLLLLLLA VSTLDFVVGS FTHLDPEHGF IGYSPELLQS NILPEYSPGE SFFTVFGVFF 250 260 270 280 290 300 PAATGVMAGF NMGGDLRDPA DSVPLGSLAA VGVSWFLYII FAFLLGAVCT REALRSDFLI 310 320 330 340 350 360 AEKVSLVGFL FLLGLYISSL ASCMGGLYGA PRILQCIAQD KVIPALAFLA NGKGPNKTPV 370 380 390 400 410 420 AAICLTSLVT MAFVLVGQVN VLAPVVTINF MLTYIMVDYS YFALSMAHCG LAPSPEPVPR 430 440 450 460 470 480 QGPDTLHCSE HLLQDRAPSY GSDVPARSLS EGTLLEFTKD MDQFLQPIEE LESRQLGSRE 490 500 510 520 530 540 GNNPKNQKRK GKKGAKQTLQ DSFLLDPGSP LSFPTRTSER LSVAFCGEQE SYQKQQTSRS 550 560 570 580 590 600 ESHDHLVPDL RNQPRVNRED FFLKCRLQEQ EIQRRPSVFY ACMCNPWVSL LGALASLLIM 610 620 630 640 650 660 FVIQWLYTLA SMGVAALVYF YIGQASPGLY LGSASNFSFF QWMKSFLVPS CRSLRSAQEQ 670 680 690 700 IILAPSPAKV DMAMTQLTQD NADFATRDRY HHSSFLSREQ LMPPY
	Isoform 2 [UniParc]. Checksum: 90FCDF8BF7ADF11E	FASTA	703	76,794
	10 20 30 40 50 60 MSQVQELFHE AAQQGILAQP QPWWKIQLFM WEPVLFGTWD GVFTSCMINI FGVVLFLRTG 70 80 90 100 110 120 WLVGNTGVLL GLLLVSFVVL VALITVLSGI GVAEHGGISS GGVYSMISSV LGGQMGGTVG 130 140 150 160 170 180 LLYVFGQCVA GAMYITGFAE SISDLLGLGD IWAVRGISVA VLLALLGINL AGVKWIIRLQ 190 200 210 220 230 240 LLLLLLLAVS TLDFVVGSFT HLDPEHGFIG YSPELLQSNI LPEYSPGESF FTVFGVFFPA 250 260 270 280 290 300 ATGVMAGFNM GGDLRDPADS VPLGSLAAVG VSWFLYIIFA FLLGAVCTRE ALRSDFLIAE 310 320 330 340 350 360 KVSLVGFLFL LGLYISSLAS CMGGLYGAPR ILQCIAQDKV IPALAFLANG KGPNKTPVAA 370 380 390 400 410 420 ICLTSLVTMA FVLVGQVNVL APVVTINFML TYIMVDYSYF ALSMAHCGLA PSPEPVPRQG 430 440 450 460 470 480 PDTLHCSEHL LQDRAPSYGS DVPARSLSEG TLLEFTKDMD QFLQPIEELE SRQLGSREGN 490 500 510 520 530 540 NPKNQKRKGK KGAKQTLQDS FLLDPGSPLS FPTRTSERLS VAFCGEQESY QKQQTSRSES 550 560 570 580 590 600 HDHLVPDLRN QPRVNREDFF LKCRLQEQEI QRRPSVFYAC MCNPWVSLLG ALASLLIMFV 610 620 630 640 650 660 IQWLYTLASM GVAALVYFYI GQASPGLYLG SASNFSFFQW MKSFLVPSCR SLRSAQEQII 670 680 690 700 LAPSPAKVDM AMTQLTQDNA DFATRDRYHH SSFLSREQLM PPY
	Isoform 3 [UniParc]. Checksum: D0C2AED2F5DEBE7B	FASTA	625	68,291
	10 20 30 40 50 60 MWEPVLFGTW DGVFTSCMIN IFGVVLFLRT GWLVGNTGVL LGLLLVSFVV LVALITVLSG 70 80 90 100 110 120 IGVAEHGGIS SGGVYSMISS VLGGQMGGTV GLLYVFGQCV AGAMYITGFA ESISDLLGLG 130 140 150 160 170 180 DIWAVRGISV AVLLALLGIN LAGVKWIIRL QLLLLLLLAV STLDFVVGSF THLDPEHGFI 190 200 210 220 230 240 GYSPELLQSN ILPEYSPGES FFTVFGVFFP AATGVMAGFN MGGDLRDPAD SVPLGSLAAV 250 260 270 280 290 300 GVSWFLYIIF AFLLGAVCTR EALRSDFLIA EKKGPNKTPV AAICLTSLVT MAFVLVGQVN 310 320 330 340 350 360 VLAPVVTINF MLTYIMVDYS YFALSMAHCG LAPSPEPVPR QGPDTLHCSE HLLQDRAPSY 370 380 390 400 410 420 GSDVPARSLS EGTLLEFTKD MDQFLQPIEE LESRQLGSRE GNNPKNQKRK GKKGAKQTLQ 430 440 450 460 470 480 DSFLLDPGSP LSFPTRTSER LSVAFCGEQE SYQKQQTSRS ESHDHLVPDL RNQPRVNRED 490 500 510 520 530 540 FFLKCRLQEQ EIQRRPSVFY ACMCNPWVSL LGALASLLIM FVIQWLYTLA SMGVAALVYF 550 560 570 580 590 600 YIGQASPGLY LGSASNFSFF QWMKSFLVPS CRSLRSAQEQ IILAPSPAKV DMAMTQLTQD 610 620 NADFATRDRY HHSSFLSREQ LMPPY
	Isoform 4 [UniParc]. Checksum: CF8D2962EAAC808F	FASTA	410	45,717
	10 20 30 40 50 60 MAGFNMGGDL RDPADSVPLG SLAAVGVSWF LYIIFAFLLG AVCTREALRS DFLIAEKKGP 70 80 90 100 110 120 NKTPVAAICL TSLVTMAFVL VGQVNVLAPV VTINFMLTYI MVDYSYFALS MAHCGLAPSP 130 140 150 160 170 180 EPVPRQGPDT LHCSEHLLQD RAPSYGSDVP ARSLSEGTLL EFTKDMDQFL QPIEELESRQ 190 200 210 220 230 240 LGSREGNNPK NQKRKGKKGA KQTLQDSFLL DPGSPLSFPT RTSERLSVAF CGEQESYQKQ 250 260 270 280 290 300 QTSRSESHDH LVPDLRNQPR VNREDFFLKC RLQEQEIQRR PSVFYACMCN PWVSLLGALA 310 320 330 340 350 360 SLLIMFVIQW LYTLASMGVA ALVYFYIGQA SPGLYLGSAS NFSFFQWMKS FLVPSCRSLR 370 380 390 400 410 SAQEQIILAP SPAKVDMAMT QLTQDNADFA TRDRYHHSSF LSREQLMPPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of mouse CCC9 (SLC12A9), a new member of the cation-chloride cotransporter gene family." Mount D.B. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Strain: C57BL/6J. Tissue: Ovary.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF319951 mRNA. Translation: AAL37178.1. AK165080 mRNA. Translation: BAE38029.1. AC121570 Genomic DNA. No translation available. AC127412 Genomic DNA. No translation available. BC030926 mRNA. Translation: AAH30926.1.
IPI	IPI00128648. IPI00169592. IPI00867905. IPI00867927.
RefSeq	NP_001077371.1. NM_001083902.1. NP_599012.2. NM_134251.2.
UniGene	Mm.35253.
3D structure databases
ProteinModelPortal	Q8VI23.
ModBase	Search...
PTM databases
PhosphoSite	Q8VI23.
Proteomic databases
PRIDE	Q8VI23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000059056; ENSMUSP00000062337; ENSMUSG00000035506. ENSMUST00000119173; ENSMUSP00000113633; ENSMUSG00000035506. ENSMUST00000121925; ENSMUSP00000112439; ENSMUSG00000035506.
GeneID	171286.
KEGG	mmu:171286.
UCSC	uc007zad.1. mouse. uc007zae.1. mouse. uc007zag.1. mouse.
Organism-specific databases
CTD	84561.
MGI	MGI:2443672. Slc12a8.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00560000077088.
HOGENOM	HOG000045198.
HOVERGEN	HBG108430.
InParanoid	Q8VI23.
KO	K14428.
OMA	GRGPNKV.
OrthoDB	EOG4X0MS2.
Gene expression databases
ArrayExpress	Q8VI23.
Bgee	Q8VI23.
Genevestigator	Q8VI23.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. [Graphical view]
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	370967.
SOURCE	Search...

Entry information

Entry name

S12A8_MOUSE

Accession

Primary (citable) accession number: Q8VI23
Secondary accession number(s): Q3TNQ9, Q8K0M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program