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Protein

Isoaspartyl peptidase/L-asparaginase

Gene

Asrgl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has both L-asparaginase and beta-aspartyl peptidase activity. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine (By similarity). May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions.By similarity1 Publication

Miscellaneous

In vasectomized rats, autoantibodies against Asrgl1 are present.

Catalytic activityi

L-asparagine + H2O = L-aspartate + NH3.1 Publication
Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.1 Publication

Kineticsi

  1. KM=2.4 mM for L-aspartic acid beta-(7-amido-4-methylcoumarin)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei191NucleophileBy similarity1

    GO - Molecular functioni

    • asparaginase activity Source: MGI
    • beta-aspartyl-peptidase activity Source: UniProtKB

    GO - Biological processi

    • asparagine catabolic process via L-aspartate Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase, Protease

    Enzyme and pathway databases

    ReactomeiR-RNO-71182 Phenylalanine and tyrosine catabolism
    SABIO-RKQ8VI04

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoaspartyl peptidase/L-asparaginase (EC:3.4.19.51 Publication, EC:3.5.1.11 Publication)
    Alternative name(s):
    Asparaginase-like protein 1
    Asparaginase-like sperm autoantigen
    Beta-aspartyl-peptidase
    Glial asparaginase
    Isoaspartyl dipeptidase
    L-asparagine amidohydrolase
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Asrgl1
    Synonyms:Alp, Gliap, Hiob
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 1

    Organism-specific databases

    RGDi708526 Asrgl1

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004205611 – 190Isoaspartyl peptidase/L-asparaginase alpha chainAdd BLAST190
    ChainiPRO_0000420562191 – 333Isoaspartyl peptidase/L-asparaginase beta chainAdd BLAST143

    Post-translational modificationi

    Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.By similarity

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    PaxDbiQ8VI04
    PRIDEiQ8VI04

    PTM databases

    iPTMnetiQ8VI04
    PhosphoSitePlusiQ8VI04
    SwissPalmiQ8VI04

    Expressioni

    Tissue specificityi

    Present in testis, brain, liver, kidney, heart and skeletal muscle. In brain, specifically present in the astrocytic lineage. Present in sperm (at protein level).2 Publications

    Gene expression databases

    BgeeiENSRNOG00000020202
    GenevisibleiQ8VI04 RN

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and beta chain produced by autocleavage. This heterodimer may then dimerize in turn, giving rise to a heterotetramer (By similarity).By similarity

    Protein-protein interaction databases

    IntActiQ8VI04, 1 interactor
    STRINGi10116.ENSRNOP00000027459

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VI04
    SMRiQ8VI04
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni219 – 222Substrate bindingBy similarity4
    Regioni242 – 245Substrate bindingBy similarity4

    Sequence similaritiesi

    Belongs to the Ntn-hydrolase family.Curated

    Phylogenomic databases

    eggNOGiKOG1592 Eukaryota
    COG1446 LUCA
    GeneTreeiENSGT00530000063034
    HOGENOMiHOG000174613
    HOVERGENiHBG101662
    InParanoidiQ8VI04
    KOiK13051
    OMAiGITGKWP
    OrthoDBiEOG091G0CKQ
    PhylomeDBiQ8VI04
    TreeFamiTF323960

    Family and domain databases

    CDDicd04702 ASRGL1_like, 1 hit
    InterProiView protein in InterPro
    IPR033844 ASRGL1_meta
    IPR029055 Ntn_hydrolases_N
    IPR000246 Peptidase_T2
    PANTHERiPTHR10188 PTHR10188, 1 hit
    PfamiView protein in Pfam
    PF01112 Asparaginase_2, 1 hit
    SUPFAMiSSF56235 SSF56235, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VI04-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATARPSSCG RDSVPATPRA SIDVSLVVVV HGGGASNISP GRKELVSEGI
    60 70 80 90 100
    AKAATEGYNI LKAGGSAVDA VEGAVTMLEN DPEFNAGYGS VLNADGDIEM
    110 120 130 140 150
    DASIMDGKDL SAGAVSAVRC IANPVKLARL VMEKTPHCFL TGRGAEKFAA
    160 170 180 190 200
    DMGIPQTPAE KLITERTKKH LEKEKLEKGA QKADCPKNSG TVGAVALDCK
    210 220 230 240 250
    GNLAYATSTG GIVNKMVGRV GDSPCIGAGG YADNNLGAVS TTGHGESILK
    260 270 280 290 300
    VNLARLALFH VEQGKTVDEA ATLALDYMKS KLKGLGGLIL INKTGDWVAK
    310 320 330
    WTSASMPWAA VKNGKLQAGI DLCETKTRNL PTC
    Length:333
    Mass (Da):34,410
    Last modified:March 1, 2002 - v1
    Checksum:iFD0BF38DAC6F905D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF329099 mRNA Translation: AAL41029.1
    AJ427914 mRNA Translation: CAD20833.1
    AJ427915 mRNA Translation: CAD20834.1
    RefSeqiNP_659557.1, NM_145089.4
    UniGeneiRn.22774

    Genome annotation databases

    EnsembliENSRNOT00000027459; ENSRNOP00000027459; ENSRNOG00000020202
    GeneIDi246307
    KEGGirno:246307
    UCSCiRGD:708526 rat

    Similar proteinsi

    Entry informationi

    Entry nameiASGL1_RAT
    AccessioniPrimary (citable) accession number: Q8VI04
    Secondary accession number(s): Q8CG44
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: March 1, 2002
    Last modified: May 23, 2018
    This is version 97 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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