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Protein

DNA damage-inducible transcript 4 protein

Gene

Ddit4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.By similarity3 Publications

GO - Biological processi

  • apoptotic process Source: RGD
  • brain development Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • defense response to virus Source: UniProtKB-KW
  • intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  • negative regulation of intracellular signal transduction Source: UniProtKB
  • negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
  • negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB
  • negative regulation of TOR signaling Source: UniProtKB
  • neuron differentiation Source: UniProtKB
  • neuron migration Source: UniProtKB
  • neurotrophin TRK receptor signaling pathway Source: UniProtKB
  • positive regulation of neuron death Source: UniProtKB
  • response to hypoxia Source: RGD
Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-inducible transcript 4 protein
Alternative name(s):
HIF-1 responsive protein RTP801
Protein regulated in development and DNA damage response 1
Short name:
REDD-1
Gene namesi
Name:Ddit4
Synonyms:Redd1, Rtp801
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621731. Ddit4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003071991 – 229DNA damage-inducible transcript 4 proteinAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineBy similarity1
Modified residuei20PhosphothreonineBy similarity1
Modified residuei118PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by GSK3B; this promotes proteasomal degradation.By similarity
Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8VHZ9.

PTM databases

PhosphoSitePlusiQ8VHZ9.

Expressioni

Inductioni

Strongly up-regulated by hypoxia. Up-regulated by dexamethasone in skeletal muscles. Up-regulated in various cellular models of Parkinson disease (at protein level).4 Publications

Interactioni

Subunit structurei

Monomer. Interacts with BTRC. Identified in a complex with CUL4A, DDB1 and BTRC. Interacts with TXNIP; this inhibits the proteasomal degradation of DDIT4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000703.

Family & Domainsi

Sequence similaritiesi

Belongs to the DDIT4 family.Curated

Phylogenomic databases

eggNOGiENOG410IIEE. Eukaryota.
ENOG4111N0W. LUCA.
HOGENOMiHOG000082523.
HOVERGENiHBG104439.
InParanoidiQ8VHZ9.
KOiK08270.
PhylomeDBiQ8VHZ9.

Family and domain databases

InterProiIPR012918. RTP801-like.
[Graphical view]
PANTHERiPTHR12478. PTHR12478. 1 hit.
PfamiPF07809. RTP801_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VHZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLWDRFSS SSSSSSSSRT PAADRPPRSA WGSAAREEGL DRCASLESSD
60 70 80 90 100
CESLDSSNSG FGPEEDSSYL DGVSLPDFEL LSDPEDEHLC ANLMQLLQES
110 120 130 140 150
LSQARLGSRR PARLLMPSQL LSQVGKELLR LAYSEPCGLR GALLDVCVEQ
160 170 180 190 200
GKSCHSVAQL ALDPSLVPTF QLTLVLRLDS RLWPKIQGLL SSANSSLVPG
210 220
YSQSLTLSTG FRVIKKKLYS SEQLLIEEC
Length:229
Mass (Da):24,885
Last modified:March 1, 2002 - v1
Checksum:i6513055142D7F675
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334162 mRNA. Translation: AAL38423.1.
BC062021 mRNA. Translation: AAH62021.1.
RefSeqiNP_543182.1. NM_080906.2.
UniGeneiRn.9775.

Genome annotation databases

GeneIDi140942.
KEGGirno:140942.
UCSCiRGD:621731. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334162 mRNA. Translation: AAL38423.1.
BC062021 mRNA. Translation: AAH62021.1.
RefSeqiNP_543182.1. NM_080906.2.
UniGeneiRn.9775.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000703.

PTM databases

PhosphoSitePlusiQ8VHZ9.

Proteomic databases

PaxDbiQ8VHZ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140942.
KEGGirno:140942.
UCSCiRGD:621731. rat.

Organism-specific databases

CTDi54541.
RGDi621731. Ddit4.

Phylogenomic databases

eggNOGiENOG410IIEE. Eukaryota.
ENOG4111N0W. LUCA.
HOGENOMiHOG000082523.
HOVERGENiHBG104439.
InParanoidiQ8VHZ9.
KOiK08270.
PhylomeDBiQ8VHZ9.

Miscellaneous databases

PROiQ8VHZ9.

Family and domain databases

InterProiIPR012918. RTP801-like.
[Graphical view]
PANTHERiPTHR12478. PTHR12478. 1 hit.
PfamiPF07809. RTP801_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDIT4_RAT
AccessioniPrimary (citable) accession number: Q8VHZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.