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Q8VHX6 (FLNC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Filamin-C

Short name=FLN-C
Alternative name(s):
ABP-280-like protein
ABP-L
Actin-binding-like protein
Filamin-2
Gamma-filamin
Gene names
Name:Flnc
Synonyms:Abpl, Fln2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2726 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers. Ref.6

Subunit structure

Homodimer By similarity. Interacts with FLNB, KCND2, INPPL1, ITGB1A, MYOT, MYOZ1 and MYOZ3. Interacts with sarcoglycans SGCD and SGCG. Interacts (via filament repeats 17-18, 20-21 and 24) with USP25 (isoform USP25monly). Interacts with FBLIM1 By similarity. Interacts with KY. Interacts with IGFN1. Interacts with MICALL2. Ref.5 Ref.8 Ref.9

Subcellular location

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasmcytoskeleton By similarity. CytoplasmmyofibrilsarcomereZ line By similarity. Note: A small amount localizes at membranes. In striated muscle cells, it predominantly localizes in myofibrillar Z lines, while a minor fraction localizes with subsarcolemme By similarity.

Domain

The intradomain insert is specific to FLNC and mediates the targeting to developing and mature Z lines By similarity.

During myogenesis, isoform 1 is expressed the first day, then is replaced by isoform 2.

Comprised of a NH2-terminal actin-binding domain, 24 internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation.

The filamin 20 repeat mediates interaction with XIRP1 By similarity.

Post-translational modification

Ubiquitinated by FBXL22, leading to proteasomal degradation By similarity.

Sequence similarities

Belongs to the filamin family.

Contains 1 actin-binding domain.

Contains 2 CH (calponin-homology) domains.

Contains 24 filamin repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VHX6-1)

Also known as: H1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VHX6-2)

Also known as: Delta-H1;

The sequence of this isoform differs from the canonical sequence as follows:
     1735-1767: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 27262726Filamin-C
PRO_0000087302

Regions

Domain1 – 260260Actin-binding
Domain37 – 143107CH 1
Domain160 – 260101CH 2
Repeat271 – 36999Filamin 1
Repeat371 – 46999Filamin 2
Repeat470 – 56697Filamin 3
Repeat567 – 65993Filamin 4
Repeat663 – 75997Filamin 5
Repeat760 – 862103Filamin 6
Repeat863 – 96199Filamin 7
Repeat962 – 105796Filamin 8
Repeat1058 – 115093Filamin 9
Repeat1151 – 124595Filamin 10
Repeat1246 – 1345100Filamin 11
Repeat1346 – 143893Filamin 12
Repeat1439 – 153496Filamin 13
Repeat1535 – 163197Filamin 14
Repeat1636 – 1735100Filamin 15
Repeat1760 – 185596Filamin 16
Repeat1856 – 194792Filamin 17
Repeat1948 – 203487Filamin 18
Repeat2037 – 212993Filamin 19
Repeat2212 – 230796Filamin 20
Repeat2310 – 240293Filamin 21
Repeat2404 – 249794Filamin 22
Repeat2501 – 259393Filamin 23
Repeat2631 – 272595Filamin 24
Region1736 – 175924Hinge 1
Region2163 – 224482Intradomain insert
Region2404 – 2725322Interaction with INPPL1 By similarity
Region2594 – 2726133Self-association site, tail By similarity
Region2594 – 263037Hinge 2

Amino acid modifications

Modified residue11621Phosphoserine By similarity
Modified residue22341Phosphoserine Ref.7

Natural variations

Alternative sequence1735 – 176733Missing in isoform 2.
VSP_007580

Experimental info

Sequence conflict14061C → R in AAI51098. Ref.2
Sequence conflict26061E → Q in AAF97411. Ref.4
Sequence conflict26091T → S in AAF97411. Ref.4
Sequence conflict26171K → H in AAF97411. Ref.4
Sequence conflict26311K → N in AAF97411. Ref.4
Sequence conflict2663 – 26653KAG → QAR in AAF97411. Ref.4
Sequence conflict26691M → I in AAF97411. Ref.4
Sequence conflict26951V → F in AAF97411. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (H1) [UniParc].

Last modified December 12, 2006. Version 3.
Checksum: BFBE03CBFFEE3863

FASTA2,726291,119
        10         20         30         40         50         60 
MMNNSNYSDA SGLGLVDEAD EMPSTEKDLA EDAPWKKIQQ NTFTRWCNEH LKCVGKRLTD 

        70         80         90        100        110        120 
LQRDLSDGLR LIALLEVLSQ KRMYRKFHPR PNFRQMKLEN VSVALEFLER EHIKLVSIDS 

       130        140        150        160        170        180 
KAIVDGNLKL ILGLIWTLIL HYSISMPMWE DEDDEDARKQ TPKQRLLGWI QNKVPQLPIT 

       190        200        210        220        230        240 
NFNRDWQDGK ALGALVDNCA PGLCPDWEAW DPNQPVQNAR EAMQQADDWL GVPQVIAPEE 

       250        260        270        280        290        300 
IVDPNVDEHS VMTYLSQFPK AKLKPGAPVR SKQLNPKKAI AYGPGIEPQG NTVLQPAHFT 

       310        320        330        340        350        360 
VQTVDAGVGE VLVYIEDPEG HTEEAKVVPN NDKDRTYAVS YVPKVAGLHK VTVLFAGQNI 

       370        380        390        400        410        420 
ERSPFEVNVG MALGDANKVS ARGPGLEPVG NVANKPTYFD IYTAGAGTGD VAVVIVDPQG 

       430        440        450        460        470        480 
RRDTVEVALE DKGDNTFRCT YRPVMEGPHT VHVAFAGAPI TRSPFPVHVA EACNPNACRA 

       490        500        510        520        530        540 
SGRGLQPKGV RVKEVADFKV FTKGAGSGEL KVTVKGPKGT EEPVKVREAG DGVFECEYYP 

       550        560        570        580        590        600 
VVPGKYVVTI TWGGYAIPRS PFEVQVSPEA GAQKVRAWGP GLETGQVGKS ADFVVEAIGT 

       610        620        630        640        650        660 
EVGTLGFSIE GPSQAKIECD DKGDGSCDVR YWPTEPGEYA VHVICDDEDI RDSPFIAHIQ 

       670        680        690        700        710        720 
PAPPDCFPDK VKAFGPGLEP TGCIVDRPAE FTIDARAAGK GDLKLYAQDA DGCPIDIKVI 

       730        740        750        760        770        780 
PNGDGTFRCS YVPTKPIKHT IIVSWGGVNV PKSPFRVNVG EGSHPERVKV YGPGVEKTGL 

       790        800        810        820        830        840 
KANEPTYFTV DCSEAGQGDV SIGIKCAPGV VGPVEADIDF DIIKNDNDTF TVKYTPPGAG 

       850        860        870        880        890        900 
HYTIMVLFAN QEIPASPFHI KVDPSHDASK VKAEGPGLSR TGVEVGKPTH FTVLTKGAGK 

       910        920        930        940        950        960 
AKLDVHFAGA AKGEAVRDFE IIDNHDYSYT VKYTAVQQGN MAVTVTYGGD PVPKSPFVVN 

       970        980        990       1000       1010       1020 
VAPPLDLSKV KVQGLNSKVA VGQEQAFSVN TRGAGGQGQL DVRMTSPSRR PIPCKLEPGG 

      1030       1040       1050       1060       1070       1080 
GAEAQAVRYM PPEEGPYKVD ITYDGHPVPG SPFAVEGVLP PDPSKVCAYG PGLKGGLVGT 

      1090       1100       1110       1120       1130       1140 
PAPFSIDTKG AGTGGLGLTV EGPCEAKIEC QDNGDGSCAV SYLPTEPGEY TINILFAEAH 

      1150       1160       1170       1180       1190       1200 
IPGSPFKATI QPVFDPSKVR ASGPGLERGK AGEAATFTVD CSEAGEAELT IEILSDAGVK 

      1210       1220       1230       1240       1250       1260 
AEVLIQNNAD GTYHITYSPA FPGTYTITIK YGGHPIPKFP TRVHVQPAVD TSGIKVSGPG 

      1270       1280       1290       1300       1310       1320 
VEPHGVLREV TTEFTVDARS LTATGGNHVT ARVLNPSGAK TDTYVTDNGD GTYRVQYTAY 

      1330       1340       1350       1360       1370       1380 
EEGVHLVEVL YDEVAVPKSP FRVGVTEGCD PTRVRAFGPG LEGGLVNKAN RFTVETRGAG 

      1390       1400       1410       1420       1430       1440 
TGGLGLAIEG PSEAKMSCKD NKDGSCTVEY IPFTPGDYDV NITFGGQPIP GSPFRVPVKD 

      1450       1460       1470       1480       1490       1500 
VVDPGKVKCS GPGLGTGVRA RVPQTFTVDC SQAGRAPLQV AVLGPTGVAE PVEVRDNGDG 

      1510       1520       1530       1540       1550       1560 
THTVHYTPAT DGPYTVAVKY ADQEVPRSPF KIKVLPSHDA SKVRASGPGL NASGIPASLP 

      1570       1580       1590       1600       1610       1620 
VEFTIDARDA GQGLLTVQIL DPEGKPKKAN IRDNGDGTYT VSYLPDMSGR YTITIKYGGD 

      1630       1640       1650       1660       1670       1680 
EIPYSPFRIH ALPTGDASKC LVTVSIGGHG LGACLGPRIQ IGEETVITVD AKAAGKGKVT 

      1690       1700       1710       1720       1730       1740 
CTVSTPDGAE LDVDVVENHD GTFDIYYTAP EPGKYVITIR FGGEHIPNSP FHVLACDPLP 

      1750       1760       1770       1780       1790       1800 
HVEEPAEMLQ MRQPYAPLRP GTCPTHWATE EPVVPVEPLE SMLRPFNLVI PFTVQKGELT 

      1810       1820       1830       1840       1850       1860 
GEVRMPSGKT ARPNITDNKD GTITVRYAPT EKGLHQMGIK YDGNHIPGSP LQFYVDAINS 

      1870       1880       1890       1900       1910       1920 
RHVSAYGPGL SHGMVNKPAT FTIVTKDAGE GGLSLAVEGP SKAEITCKDN KDGTCTVSYL 

      1930       1940       1950       1960       1970       1980 
PTAPGDYSII VRFDDKHIPG SPFTAKITGD DSMRTSQLNV GTSTDVSLKI TEGDLSQLTA 

      1990       2000       2010       2020       2030       2040 
SIRAPSGNEE PCLLKRLPNR HIGISFTPKE VGEHVVSVRK SGKHVTNSPF KILVGPSEIG 

      2050       2060       2070       2080       2090       2100 
DASKVRVWGK GLSEGQTFQV AEFIVDTRNA GYGGLGLSIE GPSKVDINCE DMEDGTCKVT 

      2110       2120       2130       2140       2150       2160 
YCPTEPGTYI INIKFADKHV PGSPFTVKVT GEGRMKESIT RRRQAPSIAT IGSTCDLNLK 

      2170       2180       2190       2200       2210       2220 
IPGNWFQMVS AQERLTRTFT RSSHTYTRTE RTEISKTRGG ETKREVRVEE STQVGGDPFP 

      2230       2240       2250       2260       2270       2280 
AVFGDFLGRE RLGSFGSITR QQEGEASSQD MTAQVTSPSG KTEAAEIVEG EDSAYSVRFV 

      2290       2300       2310       2320       2330       2340 
PQEMGPHTVT VKYRGQHVPG SPFQFTVGPL GEGGAHKVRA GGTGLERGVA GVPAEFSIWT 

      2350       2360       2370       2380       2390       2400 
REAGAGGLSI AVEGPSKAEI AFEDRKDGSC GVSYVVQEPG DYEVSIKFND EHIPDSPFVV 

      2410       2420       2430       2440       2450       2460 
PVASLSDDAR RLTVTSLQET GLKVNQPASF AVQLNGARGV IDARVHTPSG AVEECYVSEL 

      2470       2480       2490       2500       2510       2520 
DSDKHTIRFI PHENGVHSID VKFNGAHIPG SPFKIRVGEQ SQAGDPGLVS AYGPGLEGGT 

      2530       2540       2550       2560       2570       2580 
TGVSSEFIVN TQNAGSGALS VTIDGPSKVQ LDCRECPEGH VVTYTPMAPG NYLIAIKYGG 

      2590       2600       2610       2620       2630       2640 
PQHIVGSPFK AKVTGPRLSG GHSLHETSTV LVETVTKSSS SRGASYSSIP KFSSDASKVV 

      2650       2660       2670       2680       2690       2700 
TRGPGLSQAF VGQKNSFTVD CSKAGTNMMM VGVHGPKTPC EEVYVKHMGN RVYNVTYTVK 

      2710       2720 
EKGDYILIVK WGDESVPGSP FKVNVP 

« Hide

Isoform 2 (Delta-H1) [UniParc].

Checksum: 3CB7C227D50195F4
Show »

FASTA2,693287,365

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain and Embryo.
[3]"Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin (beta) subunits."
van Der Flier A., Kuikman I., Kramer D., Geerts D., Kreft M., Takafuta T., Shapiro S.S., Sonnenberg A.
J. Cell Biol. 156:361-376(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1687-1800 (ISOFORMS 1 AND 2), DEVELOPMENTAL STAGE.
Strain: C3H.
[4]"Filamin isogene expression during mouse myogenesis."
Chiang W., Greaser M.L., Lyons G.E.
Dev. Dyn. 217:99-108(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2535-2726.
Strain: C3H.
Tissue: Myotube.
[5]"Filamin C interacts with the muscular dystrophy KY protein and is abnormally distributed in mouse KY deficient muscle fibres."
Beatham J., Romero R., Townsend S.K.M., Hacker T., van der Ven P.F.M., Blanco G.
Hum. Mol. Genet. 13:2863-2874(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KY.
[6]"Loss of FilaminC (FLNc) results in severe defects in myogenesis and myotube structure."
Dalkilic I., Schienda J., Thompson T.G., Kunkel L.M.
Mol. Cell. Biol. 26:6522-6534(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2234, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[8]"Identification of a Z-band associated protein complex involving KY, FLNC and IGFN1."
Baker J., Riley G., Romero M.R., Haynes A.R., Hilton H., Simon M., Hancock J., Tateossian H., Ripoll V.M., Blanco G.
Exp. Cell Res. 316:1856-1870(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IGFN1.
[9]"Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins."
Sakane A., Alamir Mahmoud Abdallah A., Nakano K., Honda K., Kitamura T., Imoto I., Matsushita N., Sasaki T.
Genes Cells 18:810-822(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MICALL2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC044807 Genomic DNA. No translation available.
BC052186 mRNA. Translation: AAH52186.1.
BC060276 mRNA. Translation: AAH60276.1.
BC151097 mRNA. Translation: AAI51098.1.
BC158128 mRNA. Translation: AAI58129.1.
AF353670 mRNA. Translation: AAL68446.1.
AF119148 mRNA. Translation: AAF97411.1.
RefSeqNP_001074654.1. NM_001081185.1.
XP_006505227.1. XM_006505164.1.
UniGeneMm.39046.

3D structure databases

ProteinModelPortalQ8VHX6.
SMRQ8VHX6. Positions 23-2726.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid213053. 5 interactions.
IntActQ8VHX6. 8 interactions.
MINTMINT-1520143.
STRING10090.ENSMUSP00000064163.

PTM databases

PhosphoSiteQ8VHX6.

Proteomic databases

PaxDbQ8VHX6.
PRIDEQ8VHX6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090474; ENSMUSP00000087960; ENSMUSG00000068699. [Q8VHX6-1]
GeneID68794.
KEGGmmu:68794.
UCSCuc009bdn.1. mouse. [Q8VHX6-1]
uc009bdo.2. mouse. [Q8VHX6-2]

Organism-specific databases

CTD2318.
MGIMGI:95557. Flnc.

Phylogenomic databases

eggNOGCOG5069.
GeneTreeENSGT00660000095431.
HOGENOMHOG000044235.
HOVERGENHBG004163.
KOK04437.
PhylomeDBQ8VHX6.

Gene expression databases

ArrayExpressQ8VHX6.
BgeeQ8VHX6.
CleanExMM_FLNC.
GenevestigatorQ8VHX6.

Family and domain databases

Gene3D1.10.418.10. 2 hits.
2.60.40.10. 24 hits.
InterProIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR003961. Fibronectin_type3.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR028559. FLN.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR11915:SF172. PTHR11915:SF172. 1 hit.
PfamPF00307. CH. 2 hits.
PF00630. Filamin. 23 hits.
[Graphical view]
SMARTSM00033. CH. 2 hits.
SM00060. FN3. 2 hits.
SM00557. IG_FLMN. 24 hits.
[Graphical view]
SUPFAMSSF47576. SSF47576. 1 hit.
SSF81296. SSF81296. 24 hits.
PROSITEPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS50194. FILAMIN_REPEAT. 24 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio327935.
PROQ8VHX6.
SOURCESearch...

Entry information

Entry nameFLNC_MOUSE
AccessionPrimary (citable) accession number: Q8VHX6
Secondary accession number(s): B2RY80 expand/collapse secondary AC list , B9EKT2, Q6PAI6, Q9JJ38
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot