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Protein

Heterogeneous nuclear ribonucleoprotein H

Gene

Hnrnph1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG) (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • nucleotide binding Source: InterPro
  • RNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to peptide Source: RGD
  • mRNA processing Source: UniProtKB-KW
  • regulation of RNA splicing Source: UniProtKB
  • RNA processing Source: RGD
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein H
Short name:
hnRNP H
Alternative name(s):
Ratsg1
Cleaved into the following chain:
Gene namesi
Name:Hnrnph1
Synonyms:Hnrph, Hnrph1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620840. Hnrnph1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: RGD
  • nucleus Source: RGD
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003671211 – 449Heterogeneous nuclear ribonucleoprotein HAdd BLAST449
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00002885572 – 449Heterogeneous nuclear ribonucleoprotein H, N-terminally processedAdd BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein H, N-terminally processedBy similarity1
Modified residuei23PhosphoserineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei63PhosphoserineBy similarity1
Modified residuei233Dimethylated arginine; alternateBy similarity1
Modified residuei233Omega-N-methylarginine; alternateBy similarity1
Modified residuei246PhosphotyrosineBy similarity1
Modified residuei310PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ8VHV7.
PeptideAtlasiQ8VHV7.
PRIDEiQ8VHV7.

2D gel databases

World-2DPAGE0004:Q8VHV7.

PTM databases

iPTMnetiQ8VHV7.
PhosphoSitePlusiQ8VHV7.

Interactioni

Subunit structurei

Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRNPH1. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with CUGBP1; the interaction is RNA-dependent. Interacts with MBNL1; the interaction in RNA-independent (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

BioGridi250865. 1 interactor.
IntActiQ8VHV7. 1 interactor.
STRINGi10116.ENSRNOP00000055962.

Structurei

3D structure databases

ProteinModelPortaliQ8VHV7.
SMRiQ8VHV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 90RRM 1PROSITE-ProRule annotationAdd BLAST80
Domaini111 – 188RRM 2PROSITE-ProRule annotationAdd BLAST78
Repeati234 – 2491-1Add BLAST16
Domaini289 – 364RRM 3PROSITE-ProRule annotationAdd BLAST76
Repeati354 – 3722-1Add BLAST19
Repeati374 – 3922-2Add BLAST19
Repeati418 – 4331-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni234 – 4332 X 16 AA Gly-rich approximate repeatsAdd BLAST200
Regioni354 – 3922 X 19 AA perfect repeatsAdd BLAST39

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ8VHV7.
KOiK12898.
PhylomeDBiQ8VHV7.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VHV7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLGAEGGEG FVVKVRGLPW SCSADEVQRF FSDCKIQNGA QGIRFIYTRE
60 70 80 90 100
GRPSGEAFVE LESEDEVKLA LKKDRETMGH RYVEVFKSNN VEMDWVLKHT
110 120 130 140 150
GPNSPDTAND GFVRLRGLPF GCSEEEIVQF FSGLEIVPNG ITLPVDFQGR
160 170 180 190 200
STGEAFVQFA SQEIAEKALK KHKERIGHRY IEIFKSSRAE VRTHYDPPRK
210 220 230 240 250
LMAMQRPGPY DRPGAGRGYN SIGRGAGFER MRRGAYGGGY GGYDDYNGYN
260 270 280 290 300
DGYGFGSDRF GRDLNYCFSG MSDHRYGDGG STFQSTTGHC VHMRGLPYRA
310 320 330 340 350
TENDIYNFFS PLNPVRVHIE TGPDGRVTGE ADVEFATHED AVAAMSKDKA
360 370 380 390 400
NMQHRYVELF LNSTAGASGG AYEHRYVELF LNSTAGASGG AYGSQMMGGM
410 420 430 440
GLSNQSSYGG PASQQLSGGY GGGYGGQSSM SGYDQVLQEN SSDFQSNIA
Note: No experimental confirmation available.
Length:449
Mass (Da):49,188
Last modified:May 29, 2007 - v2
Checksum:i59EA20C23F9E7154
GO
Isoform 2 (identifier: Q8VHV7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     354-373: Missing.

Show »
Length:429
Mass (Da):47,064
Checksum:i94EC95ABC00F1C60
GO
Isoform 3 (identifier: Q8VHV7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.

Show »
Length:372
Mass (Da):40,606
Checksum:i0716F8281A06937B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124E → K in AAI29088 (PubMed:15489334).Curated1
Sequence conflicti321T → I in AAI29088 (PubMed:15489334).Curated1
Sequence conflicti397M → L in AAI29088 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0257121 – 77Missing in isoform 3. 1 PublicationAdd BLAST77
Alternative sequenceiVSP_025713354 – 373Missing in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367468 mRNA. Translation: AAL59557.1.
AABR03073723 Genomic DNA. No translation available.
BC129087 mRNA. Translation: AAI29088.1.
RefSeqiNP_543172.1. NM_080896.1. [Q8VHV7-3]
UniGeneiRn.23677.

Genome annotation databases

GeneIDi140931.
KEGGirno:140931.
UCSCiRGD:620840. rat. [Q8VHV7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367468 mRNA. Translation: AAL59557.1.
AABR03073723 Genomic DNA. No translation available.
BC129087 mRNA. Translation: AAI29088.1.
RefSeqiNP_543172.1. NM_080896.1. [Q8VHV7-3]
UniGeneiRn.23677.

3D structure databases

ProteinModelPortaliQ8VHV7.
SMRiQ8VHV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250865. 1 interactor.
IntActiQ8VHV7. 1 interactor.
STRINGi10116.ENSRNOP00000055962.

PTM databases

iPTMnetiQ8VHV7.
PhosphoSitePlusiQ8VHV7.

2D gel databases

World-2DPAGE0004:Q8VHV7.

Proteomic databases

PaxDbiQ8VHV7.
PeptideAtlasiQ8VHV7.
PRIDEiQ8VHV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140931.
KEGGirno:140931.
UCSCiRGD:620840. rat. [Q8VHV7-1]

Organism-specific databases

CTDi3187.
RGDi620840. Hnrnph1.

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ8VHV7.
KOiK12898.
PhylomeDBiQ8VHV7.

Miscellaneous databases

PROiQ8VHV7.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRH1_RAT
AccessioniPrimary (citable) accession number: Q8VHV7
Secondary accession number(s): A1L1J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.