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Protein

Transcription factor IIIA

Gene

Gtf3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6225C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri68 – 9225C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri98 – 12326C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri130 – 15425C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18425C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri187 – 21125C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri215 – 23723C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri244 – 26926C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri275 – 29925C2H2-type 9PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIA
Short name:
TFIIIA
Gene namesi
Name:Gtf3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619746. Gtf3a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Transcription factor IIIAPRO_0000319867Add
BLAST

Proteomic databases

PaxDbiQ8VHT8.
PRIDEiQ8VHT8.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065623.

Structurei

3D structure databases

ProteinModelPortaliQ8VHT8.
SMRiQ8VHT8. Positions 37-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6225C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri68 – 9225C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri98 – 12326C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri130 – 15425C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri160 – 18425C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri187 – 21125C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri215 – 23723C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri244 – 26926C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri275 – 29925C2H2-type 9PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOVERGENiHBG017915.
InParanoidiQ8VHT8.
KOiK09191.
PhylomeDBiQ8VHT8.

Family and domain databases

Gene3Di3.30.160.60. 8 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VHT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRVSVAEA VSSLTIADAF VRACVGPAPP RPALPSRFIC SFPDCSASYN
60 70 80 90 100
KAWKLDAHLC KHTGERPFVC DYEGCGKAFI RDYHLSRHIL IHTGEKPFVC
110 120 130 140 150
ADNGCNQKFS TKSNLKKHIE RKHENPQKQY VCNFEGCKKA FKKHQQLRTH
160 170 180 190 200
QCQHTNEPLF RCTHEGCGKH FASPSRLKRH GKVHEGYLCQ KGCSFVGKTW
210 220 230 240 250
TELLKHTREA HKEEVTCTVC QKMFKRKDHL KQHMKTHAPE RDVYRCPREG
260 270 280 290 300
CARTYTTVFN LQSHILSFHE EKRPFVCEHA GCGKTFAMKQ SLMRHSVVHD
310 320 330 340 350
PDKKRMKLKV RPPRERRSLA SRLSGYVPPK GKQEPDCSLP NSTESSSSPE
360
ATMLAPAALL TVH
Length:363
Mass (Da):41,387
Last modified:February 26, 2008 - v2
Checksum:iAACA8D5B80124563
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 324PPRP → ARAG in AAL69685 (Ref. 1) Curated
Sequence conflicti39 – 391I → T in AAL69685 (Ref. 1) Curated
Sequence conflicti67 – 671P → S in AAL69685 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CB616240 mRNA. No translation available.
AF391798 mRNA. Translation: AAL69685.1.
RefSeqiNP_001107042.1. NM_001113570.1.
UniGeneiRn.195324.

Genome annotation databases

GeneIDi246299.
KEGGirno:246299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CB616240 mRNA. No translation available.
AF391798 mRNA. Translation: AAL69685.1.
RefSeqiNP_001107042.1. NM_001113570.1.
UniGeneiRn.195324.

3D structure databases

ProteinModelPortaliQ8VHT8.
SMRiQ8VHT8. Positions 37-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065623.

Proteomic databases

PaxDbiQ8VHT8.
PRIDEiQ8VHT8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246299.
KEGGirno:246299.

Organism-specific databases

CTDi2971.
RGDi619746. Gtf3a.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOVERGENiHBG017915.
InParanoidiQ8VHT8.
KOiK09191.
PhylomeDBiQ8VHT8.

Miscellaneous databases

NextBioi623698.
PROiQ8VHT8.

Family and domain databases

Gene3Di3.30.160.60. 8 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Amgen rat EST program."
    Fitzpatrick D.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-134.
    Tissue: Spinal ganglion.
  2. "cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA."
    Hanas J.S., Hocker J.R., Cheng Y.-G., Lerner M.R., Brackett D.J., Lightfoot S.A., Hanas R.J., Madhusudhan K.T., Moreland R.J.
    Gene 282:43-52(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-363.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiTF3A_RAT
AccessioniPrimary (citable) accession number: Q8VHT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: December 9, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.