Q8VHT7 (TF3A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor IIIA
Short name=TFIIIA

Gene names

Name:

Gtf3a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 9 C2H2-type zinc fingers.
Caution	It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.
Sequence caution	The sequence AAL69686.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB28476.2 differs from that shown. Reason: Erroneous initiation. The sequence BAC25209.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding RNA-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay Ref.1. Source: MGI RNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Transcription factor IIIA		PRO_0000319866
Regions
	Zinc finger	38 – 62	25	C2H2-type 1
	Zinc finger	68 – 92	25	C2H2-type 2
	Zinc finger	98 – 123	26	C2H2-type 3
	Zinc finger	130 – 154	25	C2H2-type 4
	Zinc finger	160 – 184	25	C2H2-type 5
	Zinc finger	187 – 211	25	C2H2-type 6
	Zinc finger	215 – 237	23	C2H2-type 7
	Zinc finger	244 – 269	26	C2H2-type 8
	Zinc finger	275 – 299	25	C2H2-type 9
Experimental info
	Sequence conflict	75 – 77	3	CGK → HAS in AAH32292. Ref.3
	Sequence conflict	131	1	V → L in AAL69686. Ref.1
	Sequence conflict	149 – 173	25	THQCQ…KHFAS → PISASTPASRSSGVPTRDAG STLPR in BAC25209. Ref.2
	Sequence conflict	194	1	S → P in BAC25209. Ref.2
	Sequence conflict	198	1	K → I in BAC25209. Ref.2
	Sequence conflict	359	1	A → T in AAH32292. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8VHT7 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: FEC01712C5C8D4DA	FASTA	364	41,571
	10 20 30 40 50 60 MEPRVSVAEA VSSLTIADAF VGACEGPAPP RPALPSRFIC SFPDCSASYN KAWKLDAHLC 70 80 90 100 110 120 KHTGERPFVC DYEGCGKAFI RDYHLSRHVL IHTGEKPFVC ADDGCNQKFN TKSNLKKHIE 130 140 150 160 170 180 RKHGNPQKQY VCSYEGCKKA FKKHQQLRTH QCQHTSEPLF RCTHEGCGKH FASPSRLKRH 190 200 210 220 230 240 GKVHEGYLCQ KGCSFMGKTW TELLKHMREA HKEDITCNVC QRMFKRRDYL KQHMKTHAPE 250 260 270 280 290 300 RDVYRCPRQG CGRTYTTVFN LQSHILSFHE EKRPFVCEHA GCGKTFAMKQ SLMRHSVVHD 310 320 330 340 350 360 PDKKRMKLKV RAPRERRSLA SRLSGYFPPK RKQEPDYSLP NASAESSSSP EAQLPPPAAL LTVC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA." Hanas J.S., Hocker J.R., Cheng Y.-G., Lerner M.R., Brackett D.J., Lightfoot S.A., Hanas R.J., Madhusudhan K.T., Moreland R.J. Gene 282:43-52(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Small intestine.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-364. Strain: FVB/N-3. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF391799 mRNA. Translation: AAL69686.1. Different initiation. AK008247 mRNA. Translation: BAC25209.1. Different initiation. AK012797 mRNA. Translation: BAB28476.2. Different initiation. BC032292 mRNA. Translation: AAH32292.1.
IPI	IPI00880317.
RefSeq	NP_079928.2. NM_025652.3.
UniGene	Mm.196528.
3D structure databases
ProteinModelPortal	Q8VHT7.
SMR	Q8VHT7. Positions 37-333.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000119607.
PTM databases
PhosphoSite	Q8VHT7.
Proteomic databases
PRIDE	Q8VHT7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	66596.
KEGG	mmu:66596.
UCSC	uc009ano.2. mouse.
Organism-specific databases
CTD	2971.
MGI	MGI:1913846. Gtf3a.
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000207184.
HOVERGEN	HBG017915.
InParanoid	Q8VHT7.
KO	K09191.
Gene expression databases
CleanEx	MM_GTF3A.
Genevestigator	Q8VHT7.
Family and domain databases
Gene3D	3.30.160.60. 8 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 3 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 9 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 8 hits. PS50157. ZINC_FINGER_C2H2_2. 8 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	322116.
SOURCE	Search...

Entry information

Entry name

TF3A_MOUSE

Accession

Primary (citable) accession number: Q8VHT7
Secondary accession number(s): Q8BJ79, Q8K270, Q9CSH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	May 29, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program