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Protein

Arsenite methyltransferase

Gene

As3mt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH.

Catalytic activityi

S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate.
S-adenosyl-L-methionine + methylarsonite = S-adenosyl-L-homocysteine + dimethylarsinate.

GO - Molecular functioni

  • arsenite methyltransferase activity Source: UniProtKB
  • methylarsonite methyltransferase activity Source: UniProtKB
  • protein methyltransferase activity Source: RGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: RGD

GO - Biological processi

  • arsonoacetate metabolic process Source: UniProtKB
  • response to arsenic-containing substance Source: MGI
  • toxin metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.137. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite methyltransferase (EC:2.1.1.137)
Alternative name(s):
Methylarsonite methyltransferase
S-adenosyl-L-methionine:arsenic(III) methyltransferase
Gene namesi
Name:As3mt
Synonyms:Cyt19
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621325. As3mt.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi156 – 1561C → S: Complete loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Arsenite methyltransferasePRO_0000204449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VHT6.
PRIDEiQ8VHT6.

PTM databases

iPTMnetiQ8VHT6.
PhosphoSiteiQ8VHT6.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027223.

Structurei

3D structure databases

ProteinModelPortaliQ8VHT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410IFW3. Eukaryota.
ENOG410XSKB. LUCA.
HOGENOMiHOG000229966.
HOVERGENiHBG050585.
InParanoidiQ8VHT6.
KOiK07755.
PhylomeDBiQ8VHT6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026669. Arsenite_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10108:SF883. PTHR10108:SF883. 1 hit.
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8VHT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPRDAEIH KDVQNYYGNV LKTSADLQTN ACVTPAKGVP EYIRKSLQNV
60 70 80 90 100
HEEVISRYYG CGLVVPEHLE NCRILDLGSG SGRDCYVLSQ LVGQKGHITG
110 120 130 140 150
IDMTKVQVEV AKAYLEYHTE KFGFQTPNVT FLHGQIEMLA EAGIQKESYD
160 170 180 190 200
IVISNCVINL VPDKQKVLRE VYQVLKYGGE LYFSDVYASL EVSEDIKSHK
210 220 230 240 250
VLWGECLGGA LYWKDLAVIA KKIGFCPPRL VTANIITVGN KELERVLGDC
260 270 280 290 300
RFVSATFRLF KLPKTEPAGR CQVVYNGGIM GHEKELIFDA NFTFKEGEAV
310 320 330 340 350
EVDEETAAIL RNSRFAHDFL FTPVEASLLA PQTKVIIRDP FKLAEESDKM
360
KPRCAPEGTG GCCGKRKSC
Length:369
Mass (Da):41,056
Last modified:March 1, 2002 - v1
Checksum:iBD092F7317B138E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393243 mRNA. Translation: AAL61609.1.
RefSeqiNP_543166.1. NM_080890.1.
UniGeneiRn.95453.

Genome annotation databases

GeneIDi140925.
KEGGirno:140925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393243 mRNA. Translation: AAL61609.1.
RefSeqiNP_543166.1. NM_080890.1.
UniGeneiRn.95453.

3D structure databases

ProteinModelPortaliQ8VHT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027223.

PTM databases

iPTMnetiQ8VHT6.
PhosphoSiteiQ8VHT6.

Proteomic databases

PaxDbiQ8VHT6.
PRIDEiQ8VHT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140925.
KEGGirno:140925.

Organism-specific databases

CTDi57412.
RGDi621325. As3mt.

Phylogenomic databases

eggNOGiENOG410IFW3. Eukaryota.
ENOG410XSKB. LUCA.
HOGENOMiHOG000229966.
HOVERGENiHBG050585.
InParanoidiQ8VHT6.
KOiK07755.
PhylomeDBiQ8VHT6.

Enzyme and pathway databases

BRENDAi2.1.1.137. 5301.

Miscellaneous databases

PROiQ8VHT6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026669. Arsenite_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10108:SF883. PTHR10108:SF883. 1 hit.
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiAS3MT_RAT
AccessioniPrimary (citable) accession number: Q8VHT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.