Q8VHR5 (P66B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional repressor p66-beta

Alternative name(s):
GATA zinc finger domain-containing protein 2B
p66/p68

Gene names

Name:

Gatad2b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A By similarity. Targets MBD3 to discrete loci in the nucleus By similarity.
Subunit structure	Binds MBD2 and MBD3. Interaction with MBD2 is required for the enhancement of MBD2-mediated repression and for targeting to the chromatin By similarity. Component of the MeCP1 histone deacetylase complex By similarity. Interacts with histone tails, including that of histones H2A, H2B, H3 and H4 By similarity.
Subcellular location	Nucleus speckle By similarity. Note: Speckled nuclear localization requires both CR1 and CR2 regions By similarity.
Domain	Both CR1 and CR2 regions are required for speckled nuclear localization By similarity.
Sequence similarities	Contains 1 GATA-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VHR5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VHR5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     475-485: EIEQRLQQQAA → VRTLTPTCTVI
     486-594: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Transcriptional repressor p66-beta		PRO_0000083503
Regions
	Zinc finger	415 – 468	54	GATA-type
	Region	166 – 191	26	CR1
	Region	341 – 481	141	CR2
	Coiled coil	141 – 195	55	Potential
	Coiled coil	450 – 483	34	Potential
Amino acid modifications
	Modified residue	121	1	Phosphothreonine Ref.4 Ref.5 Ref.6
	Modified residue	123	1	Phosphoserine Ref.4 Ref.5 Ref.6
	Modified residue	130	1	Phosphoserine Ref.4
	Modified residue	135	1	Phosphoserine By similarity
	Modified residue	136	1	Phosphoserine Ref.4
	Modified residue	334	1	Phosphoserine By similarity
	Modified residue	339	1	Phosphoserine By similarity
	Modified residue	487	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	475 – 485	11	EIEQRLQQQAA → VRTLTPTCTVI in isoform 2.		VSP_010930
	Alternative sequence	486 – 594	109	Missing in isoform 2.		VSP_010931

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C57330259EADAAE6	FASTA	594	65,411
	10 20 30 40 50 60 MDRMTEDALR LNLLKRSLDP ADERDDVLAK RLKMEGHEAM ERLKMLALLK RKDLANLEVP 70 80 90 100 110 120 HELPTKQDGS GVKGYEEKLN GNLRPHGDNN RTAGRPGKEN INDEPVDMSA RRSEPDRGRL 130 140 150 160 170 180 TPSPDIIVLS DNEASSPRSS SRMEERLKAA NLEMFKGKGM EERQQLIKQL RDELRLEEAR 190 200 210 220 230 240 LVLLKKLRQS QLQKENVVQK TPVVQNAASI VQPSPAHVGQ QGLSKLPSRP GAQGIEPQNM 250 260 270 280 290 300 RTLQGHSVIR SATNTTLPHM LMSQRVIAPN PAQLQGQRGP PKPGIVRTTT PNMNPAISYQ 310 320 330 340 350 360 PQSSSSVPCQ RTTSSAIYMN LASHIQPGTV NRVSSPLPSP SAMSDAANSQ AAAKLALRKQ 370 380 390 400 410 420 LEKTLLEIPP PKPPAPLLHF LPSAANSEFI YMVGLEEVVQ SVIDSQGKNC ASLLRVEPFV 430 440 450 460 470 480 CAQCRTDFTP HWKQEKNGKI LCEQCMTSNQ KKALKAEHTN RLKNAFVKAL QQEQEIEQRL 490 500 510 520 530 540 QQQAALSPTT APAVSSVSKQ ETIMRHHTLR QAPQPQSSLQ RGIPTSARSM LSNFAQAPQL 550 560 570 580 590 SVPGGLLGMP GVNIAYLNTG IGGHKAPSLA DRQREYLLDM IPPRSISQSI SGQK
	Isoform 2 [UniParc]. Checksum: 319D9E907558D734	FASTA	485	53,734
	10 20 30 40 50 60 MDRMTEDALR LNLLKRSLDP ADERDDVLAK RLKMEGHEAM ERLKMLALLK RKDLANLEVP 70 80 90 100 110 120 HELPTKQDGS GVKGYEEKLN GNLRPHGDNN RTAGRPGKEN INDEPVDMSA RRSEPDRGRL 130 140 150 160 170 180 TPSPDIIVLS DNEASSPRSS SRMEERLKAA NLEMFKGKGM EERQQLIKQL RDELRLEEAR 190 200 210 220 230 240 LVLLKKLRQS QLQKENVVQK TPVVQNAASI VQPSPAHVGQ QGLSKLPSRP GAQGIEPQNM 250 260 270 280 290 300 RTLQGHSVIR SATNTTLPHM LMSQRVIAPN PAQLQGQRGP PKPGIVRTTT PNMNPAISYQ 310 320 330 340 350 360 PQSSSSVPCQ RTTSSAIYMN LASHIQPGTV NRVSSPLPSP SAMSDAANSQ AAAKLALRKQ 370 380 390 400 410 420 LEKTLLEIPP PKPPAPLLHF LPSAANSEFI YMVGLEEVVQ SVIDSQGKNC ASLLRVEPFV 430 440 450 460 470 480 CAQCRTDFTP HWKQEKNGKI LCEQCMTSNQ KKALKAEHTN RLKNAFVKAL QQEQVRTLTP TCTVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and functional characterization of the p66/p68 components of the MeCP1 complex." Feng Q., Cao R., Xia L., Erdjument-Bromage H., Tempst P., Zhang Y. Mol. Cell. Biol. 22:536-546(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Thymus.
[3]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-487, MASS SPECTROMETRY. Tissue: Embryonic brain.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-121; SER-123; SER-130 AND SER-136, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-121 AND SER-123, MASS SPECTROMETRY. Tissue: Melanoma.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-121 AND SER-123, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF411837 mRNA. Translation: AAL39081.1. AK041594 mRNA. Translation: BAC30997.1.
IPI	IPI00128615. IPI00453989.
RefSeq	NP_647465.1. NM_139304.1.
UniGene	Mm.270999.
3D structure databases
ProteinModelPortal	Q8VHR5.
SMR	Q8VHR5. Positions 161-200.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8VHR5. 1 interaction.
STRING	10090.ENSMUSP00000041370.
PTM databases
PhosphoSite	Q8VHR5.
Proteomic databases
PaxDb	Q8VHR5.
PRIDE	Q8VHR5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049382; ENSMUSP00000041370; ENSMUSG00000042390.
GeneID	229542.
KEGG	mmu:229542.
UCSC	uc008qca.1. mouse. uc008qcb.1. mouse.
Organism-specific databases
CTD	57459.
MGI	MGI:2443225. Gatad2b.
Phylogenomic databases
eggNOG	NOG293566.
GeneTree	ENSGT00390000004097.
HOGENOM	HOG000074070.
HOVERGEN	HBG053401.
InParanoid	Q8VHR5.
OMA	PCQRTTS.
OrthoDB	EOG4CVG6R.
Gene expression databases
ArrayExpress	Q8VHR5.
Bgee	Q8VHR5.
CleanEx	MM_GATAD2B.
Genevestigator	Q8VHR5.
GermOnline	ENSMUSG00000042390. Mus musculus.
Family and domain databases
Gene3D	3.30.50.10. 1 hit.
InterPro	IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00320. GATA. 1 hit. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. False negative. PS50114. GATA_ZN_FINGER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GATAD2B. mouse.
NextBio	379495.
SOURCE	Search...

Entry information

Entry name

P66B_MOUSE

Accession

Primary (citable) accession number: Q8VHR5
Secondary accession number(s): Q8C9Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program