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Q8VHQ7

- SYTL4_RAT

UniProt

Q8VHQ7 - SYTL4_RAT

Protein

Synaptotagmin-like protein 4

Gene

Sytl4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri63 – 10543FYVE-typeAdd
    BLAST

    GO - Molecular functioni

    1. neurexin family protein binding Source: UniProtKB
    2. phospholipid binding Source: UniProtKB
    3. protein binding Source: RGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. exocytosis Source: UniProtKB
    2. intracellular protein transport Source: InterPro

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptotagmin-like protein 4
    Alternative name(s):
    Exophilin-2
    Granuphilin
    Gene namesi
    Name:Sytl4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620204. Sytl4.

    Subcellular locationi

    Membrane 1 Publication; Peripheral membrane protein 1 Publication. Cytoplasmic vesiclesecretory vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication
    Note: Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles.

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. extrinsic component of membrane Source: UniProtKB
    3. secretory granule membrane Source: RGD
    4. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi21 – 211V → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. 1 Publication
    Mutagenesisi43 – 431L → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 672672Synaptotagmin-like protein 4PRO_0000190218Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei204 – 2041PhosphoserineBy similarity
    Modified residuei217 – 2171PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8VHQ7.
    PRIDEiQ8VHQ7.

    Expressioni

    Tissue specificityi

    Detected in insulin-secreting cell lines.

    Gene expression databases

    GenevestigatoriQ8VHQ7.

    Interactioni

    Subunit structurei

    Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B. Interacts with MYO5A By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8VHQ7. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VHQ7.
    SMRiQ8VHQ7. Positions 43-106.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 122119RabBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini359 – 463105C2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini514 – 618105C2 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi305 – 3128Poly-Glu

    Sequence similaritiesi

    Contains 2 C2 domains.PROSITE-ProRule annotation
    Contains 1 FYVE-type zinc finger.Curated
    Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri63 – 10543FYVE-typeAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG293068.
    HOGENOMiHOG000231332.
    HOVERGENiHBG054255.
    InParanoidiQ8VHQ7.
    KOiK17598.
    PhylomeDBiQ8VHQ7.

    Family and domain databases

    Gene3Di2.60.40.150. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR028694. SYTL4.
    IPR010911. Znf_FYVE-typ.
    IPR011011. Znf_FYVE_PHD.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PANTHERiPTHR10024:SF9. PTHR10024:SF9. 1 hit.
    PfamiPF00168. C2. 2 hits.
    PF02318. FYVE_2. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 2 hits.
    SM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 2 hits.
    SSF57903. SSF57903. 1 hit.
    PROSITEiPS50004. C2. 2 hits.
    PS50916. RABBD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VHQ7-1) [UniParc]FASTAAdd to Basket

    Also known as: Granuphilin-a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSEILDLSFL SEMERDLILS VLQRDEELRK ADEKRIRRLK NELLEIKRKG    50
    AKRGSQHYSD RTCARCQEGL GRLISKSNTC VGCNHLVCRE CRVLESNGSW 100
    RCKVCSKEIE LKKATGDWFY DQKVNRFAYR TGSDIIRMSL RRKPAVNKRE 150
    TVGQSLLQQT QMGDIWPGRR IIQEQQKEQS VLFEVPKLKS GKSALEAESE 200
    SLDSYTADSD STSRRDSLDK SGLFPEWKKM SAPKSQVEKE IAPGNQNAVC 250
    GDEGDMIFKK NTRKVLRPSE YTKSVIDLRP EDVAQESGIL GDRSKSVPGL 300
    SVDMEDEEEE EEDIDHLVKL HRQKLARGSM QSGSSMSTIG SMMSLYSEAG 350
    DFGNVSVTGK IAFSLKFEQK TQTLVIHVKE CHQLAYADEA KKRSNPYVKT 400
    YLLPDKSRQG KRKTSIKRDT INPLYDETFR YEISESLLAQ RTLQFSVWHH 450
    GRFGRNTFLG EAEVHMDSWK LDKKLDHCLP LHGKISTESS PGLPAHKGEL 500
    VVSLKYIPAS KLPVGGDRKK SKGGEGGELQ VWIKEAKNLT AAKSGGTSDS 550
    FVKGYLLPMR NKASKRKTPV MKKTLNPHYN HTFVYNGVRL EDLQHMCLEL 600
    TVWDREPLAS NDFLGGVRLG VGTGISSGEV VDWMDSTGEE VSLWQKMRQY 650
    PGSWAEGTLQ LRSSMVKQKL GV 672
    Length:672
    Mass (Da):75,900
    Last modified:March 1, 2002 - v1
    Checksum:i0F994F4D85202400
    GO
    Isoform 2 (identifier: Q8VHQ7-2) [UniParc]FASTAAdd to Basket

    Also known as: Granuphilin-b

    The sequence of this isoform differs from the canonical sequence as follows:
         485-501: ISTESSPGLPAHKGELV → GSVVAKWWTGWIRLVKK
         502-672: Missing.

    Show »
    Length:501
    Mass (Da):57,326
    Checksum:iB2F11B652A96AE27
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei485 – 50117ISTES…KGELV → GSVVAKWWTGWIRLVKK in isoform 2. 1 PublicationVSP_007903Add
    BLAST
    Alternative sequencei502 – 672171Missing in isoform 2. 1 PublicationVSP_007904Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF419341 mRNA. Translation: AAL38513.1.
    AF419342 mRNA. Translation: AAL38514.1.
    RefSeqiNP_536335.1. NM_080410.1. [Q8VHQ7-1]
    UniGeneiRn.107417.

    Genome annotation databases

    GeneIDi140594.
    KEGGirno:140594.
    UCSCiRGD:620204. rat. [Q8VHQ7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF419341 mRNA. Translation: AAL38513.1 .
    AF419342 mRNA. Translation: AAL38514.1 .
    RefSeqi NP_536335.1. NM_080410.1. [Q8VHQ7-1 ]
    UniGenei Rn.107417.

    3D structure databases

    ProteinModelPortali Q8VHQ7.
    SMRi Q8VHQ7. Positions 43-106.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VHQ7. 1 interaction.

    Proteomic databases

    PaxDbi Q8VHQ7.
    PRIDEi Q8VHQ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 140594.
    KEGGi rno:140594.
    UCSCi RGD:620204. rat. [Q8VHQ7-1 ]

    Organism-specific databases

    CTDi 94121.
    RGDi 620204. Sytl4.

    Phylogenomic databases

    eggNOGi NOG293068.
    HOGENOMi HOG000231332.
    HOVERGENi HBG054255.
    InParanoidi Q8VHQ7.
    KOi K17598.
    PhylomeDBi Q8VHQ7.

    Miscellaneous databases

    NextBioi 620570.
    PROi Q8VHQ7.

    Gene expression databases

    Genevestigatori Q8VHQ7.

    Family and domain databases

    Gene3Di 2.60.40.150. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR028694. SYTL4.
    IPR010911. Znf_FYVE-typ.
    IPR011011. Znf_FYVE_PHD.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    PANTHERi PTHR10024:SF9. PTHR10024:SF9. 1 hit.
    Pfami PF00168. C2. 2 hits.
    PF02318. FYVE_2. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 2 hits.
    SM00184. RING. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 2 hits.
    SSF57903. SSF57903. 1 hit.
    PROSITEi PS50004. C2. 2 hits.
    PS50916. RABBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pancreatic beta-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosis."
      Coppola T., Frantz C., Perret-Menoud V., Gattesco S., Hirling H., Regazzi R.
      Mol. Biol. Cell 13:1906-1915(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF VAL-21 AND LEU-43, INTERACTION WITH RAB3A AND STXBP1, SUBCELLULAR LOCATION.
      Tissue: Insulinoma.

    Entry informationi

    Entry nameiSYTL4_RAT
    AccessioniPrimary (citable) accession number: Q8VHQ7
    Secondary accession number(s): Q8VHQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3