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Q8VHQ7 (SYTL4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Synaptotagmin-like protein 4
Alternative name(s):
Exophilin-2
Granuphilin
Gene names
Name:Sytl4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length672 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner By similarity.

Subunit structure

Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B By similarity.

Subcellular location

Membrane; Peripheral membrane protein. Cytoplasmic vesicle › secretory vesicle membrane; Peripheral membrane protein. Note: Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles. Ref.1

Tissue specificity

Detected in insulin-secreting cell lines.

Sequence similarities

Contains 2 C2 domains.

Contains 1 FYVE-type zinc finger.

Contains 1 RabBD (Rab-binding) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VHQ7-1)

Also known as: Granuphilin-a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VHQ7-2)

Also known as: Granuphilin-b;

The sequence of this isoform differs from the canonical sequence as follows:
     485-501: ISTESSPGLPAHKGELV → GSVVAKWWTGWIRLVKK
     502-672: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 672672Synaptotagmin-like protein 4
PRO_0000190218

Regions

Domain4 – 122119RabBD
Domain359 – 463105C2 1
Domain514 – 618105C2 2
Zinc finger63 – 10543FYVE-type
Compositional bias305 – 3128Poly-Glu

Amino acid modifications

Modified residue2041Phosphoserine By similarity
Modified residue2171Phosphoserine By similarity

Natural variations

Alternative sequence485 – 50117ISTES…KGELV → GSVVAKWWTGWIRLVKK in isoform 2.
VSP_007903
Alternative sequence502 – 672171Missing in isoform 2.
VSP_007904

Experimental info

Mutagenesis211V → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. Ref.1
Mutagenesis431L → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Granuphilin-a) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 0F994F4D85202400

FASTA67275,900
        10         20         30         40         50         60 
MSEILDLSFL SEMERDLILS VLQRDEELRK ADEKRIRRLK NELLEIKRKG AKRGSQHYSD 

        70         80         90        100        110        120 
RTCARCQEGL GRLISKSNTC VGCNHLVCRE CRVLESNGSW RCKVCSKEIE LKKATGDWFY 

       130        140        150        160        170        180 
DQKVNRFAYR TGSDIIRMSL RRKPAVNKRE TVGQSLLQQT QMGDIWPGRR IIQEQQKEQS 

       190        200        210        220        230        240 
VLFEVPKLKS GKSALEAESE SLDSYTADSD STSRRDSLDK SGLFPEWKKM SAPKSQVEKE 

       250        260        270        280        290        300 
IAPGNQNAVC GDEGDMIFKK NTRKVLRPSE YTKSVIDLRP EDVAQESGIL GDRSKSVPGL 

       310        320        330        340        350        360 
SVDMEDEEEE EEDIDHLVKL HRQKLARGSM QSGSSMSTIG SMMSLYSEAG DFGNVSVTGK 

       370        380        390        400        410        420 
IAFSLKFEQK TQTLVIHVKE CHQLAYADEA KKRSNPYVKT YLLPDKSRQG KRKTSIKRDT 

       430        440        450        460        470        480 
INPLYDETFR YEISESLLAQ RTLQFSVWHH GRFGRNTFLG EAEVHMDSWK LDKKLDHCLP 

       490        500        510        520        530        540 
LHGKISTESS PGLPAHKGEL VVSLKYIPAS KLPVGGDRKK SKGGEGGELQ VWIKEAKNLT 

       550        560        570        580        590        600 
AAKSGGTSDS FVKGYLLPMR NKASKRKTPV MKKTLNPHYN HTFVYNGVRL EDLQHMCLEL 

       610        620        630        640        650        660 
TVWDREPLAS NDFLGGVRLG VGTGISSGEV VDWMDSTGEE VSLWQKMRQY PGSWAEGTLQ 

       670 
LRSSMVKQKL GV

« Hide

Isoform 2 (Granuphilin-b) [UniParc].

Checksum: B2F11B652A96AE27
Show »

FASTA50157,326

References

[1]"Pancreatic beta-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosis."
Coppola T., Frantz C., Perret-Menoud V., Gattesco S., Hirling H., Regazzi R.
Mol. Biol. Cell 13:1906-1915(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF VAL-21 AND LEU-43, INTERACTION WITH RAB3A AND STXBP1, SUBCELLULAR LOCATION.
Tissue: Insulinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF419341 mRNA. Translation: AAL38513.1.
AF419342 mRNA. Translation: AAL38514.1.
IPIIPI00207254.
IPI00207255.
RefSeqNP_536335.1. NM_080410.1.
UniGeneRn.107417.

3D structure databases

ProteinModelPortalQ8VHQ7.
SMRQ8VHQ7. Positions 43-106.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8VHQ7. 1 interaction.

Proteomic databases

PaxDbQ8VHQ7.
PRIDEQ8VHQ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID140594.
KEGGrno:140594.
UCSCRGD:620204. rat.

Organism-specific databases

CTD94121.
RGD620204. Sytl4.

Phylogenomic databases

eggNOGNOG293068.
HOGENOMHOG000231332.
HOVERGENHBG054255.
InParanoidQ8VHQ7.
OrthoDBEOG4Z62NS.

Gene expression databases

ArrayExpressQ8VHQ7.
GenevestigatorQ8VHQ7.
GermOnlineENSRNOG00000003526. Rattus norvegicus.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR018029. C2_membr_targeting.
IPR010911. Rab-bd_domain.
IPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00168. C2. 2 hits.
[Graphical view]
SMARTSM00239. C2. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF49562. C2_CaLB. 2 hits.
SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITEPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio620570.

Entry information

Entry nameSYTL4_RAT
AccessionPrimary (citable) accession number: Q8VHQ7
Secondary accession number(s): Q8VHQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 1, 2002
Last modified: April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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