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Protein

Synaptotagmin-like protein 4

Gene

Sytl4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 105FYVE-typeAdd BLAST43

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-like protein 4
Alternative name(s):
Exophilin-2
Granuphilin
Gene namesi
Name:Sytl4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620204. Sytl4.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • exocytic vesicle Source: GO_Central
  • extrinsic component of membrane Source: UniProtKB
  • presynapse Source: GOC
  • secretory granule membrane Source: RGD
  • transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21V → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. 1 Publication1
Mutagenesisi43L → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902181 – 672Synaptotagmin-like protein 4Add BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphoserineBy similarity1
Modified residuei204PhosphoserineBy similarity1
Modified residuei217PhosphoserineCombined sources1
Modified residuei221PhosphoserineBy similarity1
Modified residuei274PhosphoserineCombined sources1
Modified residuei489PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VHQ7.
PRIDEiQ8VHQ7.

PTM databases

iPTMnetiQ8VHQ7.
PhosphoSitePlusiQ8VHQ7.

Expressioni

Tissue specificityi

Detected in insulin-secreting cell lines.

Interactioni

Subunit structurei

Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B. Interacts with MYO5A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250808. 1 interactor.
IntActiQ8VHQ7. 1 interactor.
STRINGi10116.ENSRNOP00000004909.

Structurei

3D structure databases

ProteinModelPortaliQ8VHQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 122RabBDPROSITE-ProRule annotationAdd BLAST119
Domaini359 – 463C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini514 – 618C2 2PROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi305 – 312Poly-Glu8

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.Curated
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 105FYVE-typeAdd BLAST43

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IT3S. Eukaryota.
ENOG410Y1U8. LUCA.
HOGENOMiHOG000231332.
HOVERGENiHBG054255.
InParanoidiQ8VHQ7.
KOiK17598.
PhylomeDBiQ8VHQ7.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028694. SYTL4.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF9. PTHR10024:SF9. 3 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VHQ7-1) [UniParc]FASTAAdd to basket
Also known as: Granuphilin-a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEILDLSFL SEMERDLILS VLQRDEELRK ADEKRIRRLK NELLEIKRKG
60 70 80 90 100
AKRGSQHYSD RTCARCQEGL GRLISKSNTC VGCNHLVCRE CRVLESNGSW
110 120 130 140 150
RCKVCSKEIE LKKATGDWFY DQKVNRFAYR TGSDIIRMSL RRKPAVNKRE
160 170 180 190 200
TVGQSLLQQT QMGDIWPGRR IIQEQQKEQS VLFEVPKLKS GKSALEAESE
210 220 230 240 250
SLDSYTADSD STSRRDSLDK SGLFPEWKKM SAPKSQVEKE IAPGNQNAVC
260 270 280 290 300
GDEGDMIFKK NTRKVLRPSE YTKSVIDLRP EDVAQESGIL GDRSKSVPGL
310 320 330 340 350
SVDMEDEEEE EEDIDHLVKL HRQKLARGSM QSGSSMSTIG SMMSLYSEAG
360 370 380 390 400
DFGNVSVTGK IAFSLKFEQK TQTLVIHVKE CHQLAYADEA KKRSNPYVKT
410 420 430 440 450
YLLPDKSRQG KRKTSIKRDT INPLYDETFR YEISESLLAQ RTLQFSVWHH
460 470 480 490 500
GRFGRNTFLG EAEVHMDSWK LDKKLDHCLP LHGKISTESS PGLPAHKGEL
510 520 530 540 550
VVSLKYIPAS KLPVGGDRKK SKGGEGGELQ VWIKEAKNLT AAKSGGTSDS
560 570 580 590 600
FVKGYLLPMR NKASKRKTPV MKKTLNPHYN HTFVYNGVRL EDLQHMCLEL
610 620 630 640 650
TVWDREPLAS NDFLGGVRLG VGTGISSGEV VDWMDSTGEE VSLWQKMRQY
660 670
PGSWAEGTLQ LRSSMVKQKL GV
Length:672
Mass (Da):75,900
Last modified:March 1, 2002 - v1
Checksum:i0F994F4D85202400
GO
Isoform 2 (identifier: Q8VHQ7-2) [UniParc]FASTAAdd to basket
Also known as: Granuphilin-b

The sequence of this isoform differs from the canonical sequence as follows:
     485-501: ISTESSPGLPAHKGELV → GSVVAKWWTGWIRLVKK
     502-672: Missing.

Show »
Length:501
Mass (Da):57,326
Checksum:iB2F11B652A96AE27
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007903485 – 501ISTES…KGELV → GSVVAKWWTGWIRLVKK in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_007904502 – 672Missing in isoform 2. 1 PublicationAdd BLAST171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419341 mRNA. Translation: AAL38513.1.
AF419342 mRNA. Translation: AAL38514.1.
RefSeqiNP_536335.1. NM_080410.1. [Q8VHQ7-1]
UniGeneiRn.107417.

Genome annotation databases

GeneIDi140594.
KEGGirno:140594.
UCSCiRGD:620204. rat. [Q8VHQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419341 mRNA. Translation: AAL38513.1.
AF419342 mRNA. Translation: AAL38514.1.
RefSeqiNP_536335.1. NM_080410.1. [Q8VHQ7-1]
UniGeneiRn.107417.

3D structure databases

ProteinModelPortaliQ8VHQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250808. 1 interactor.
IntActiQ8VHQ7. 1 interactor.
STRINGi10116.ENSRNOP00000004909.

PTM databases

iPTMnetiQ8VHQ7.
PhosphoSitePlusiQ8VHQ7.

Proteomic databases

PaxDbiQ8VHQ7.
PRIDEiQ8VHQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140594.
KEGGirno:140594.
UCSCiRGD:620204. rat. [Q8VHQ7-1]

Organism-specific databases

CTDi94121.
RGDi620204. Sytl4.

Phylogenomic databases

eggNOGiENOG410IT3S. Eukaryota.
ENOG410Y1U8. LUCA.
HOGENOMiHOG000231332.
HOVERGENiHBG054255.
InParanoidiQ8VHQ7.
KOiK17598.
PhylomeDBiQ8VHQ7.

Miscellaneous databases

PROiQ8VHQ7.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028694. SYTL4.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF9. PTHR10024:SF9. 3 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYTL4_RAT
AccessioniPrimary (citable) accession number: Q8VHQ7
Secondary accession number(s): Q8VHQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.