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Q8VHQ7

- SYTL4_RAT

UniProt

Q8VHQ7 - SYTL4_RAT

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Protein

Synaptotagmin-like protein 4

Gene

Sytl4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri63 – 10543FYVE-typeAdd
BLAST

GO - Molecular functioni

  1. neurexin family protein binding Source: UniProtKB
  2. phospholipid binding Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. exocytosis Source: UniProtKB
  2. intracellular protein transport Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-like protein 4
Alternative name(s):
Exophilin-2
Granuphilin
Gene namesi
Name:Sytl4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620204. Sytl4.

Subcellular locationi

Membrane 1 Publication; Peripheral membrane protein 1 Publication. Cytoplasmic vesiclesecretory vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles.

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. extrinsic component of membrane Source: UniProtKB
  3. secretory granule membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 211V → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. 1 Publication
Mutagenesisi43 – 431L → A: Strongly reduces interaction with RAB3A and modulation of exocytosis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 672672Synaptotagmin-like protein 4PRO_0000190218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei204 – 2041PhosphoserineBy similarity
Modified residuei217 – 2171PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VHQ7.
PRIDEiQ8VHQ7.

Expressioni

Tissue specificityi

Detected in insulin-secreting cell lines.

Gene expression databases

GenevestigatoriQ8VHQ7.

Interactioni

Subunit structurei

Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B. Interacts with MYO5A (By similarity).By similarity

Protein-protein interaction databases

BioGridi250808. 1 interaction.
IntActiQ8VHQ7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8VHQ7.
SMRiQ8VHQ7. Positions 43-106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 122119RabBDPROSITE-ProRule annotationAdd
BLAST
Domaini359 – 463105C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini514 – 618105C2 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi305 – 3128Poly-Glu

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.Curated
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri63 – 10543FYVE-typeAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG293068.
HOGENOMiHOG000231332.
HOVERGENiHBG054255.
InParanoidiQ8VHQ7.
KOiK17598.
PhylomeDBiQ8VHQ7.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR028694. SYTL4.
IPR010911. Znf_FYVE-typ.
IPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF9. PTHR10024:SF9. 1 hit.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VHQ7-1) [UniParc]FASTAAdd to Basket

Also known as: Granuphilin-a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEILDLSFL SEMERDLILS VLQRDEELRK ADEKRIRRLK NELLEIKRKG
60 70 80 90 100
AKRGSQHYSD RTCARCQEGL GRLISKSNTC VGCNHLVCRE CRVLESNGSW
110 120 130 140 150
RCKVCSKEIE LKKATGDWFY DQKVNRFAYR TGSDIIRMSL RRKPAVNKRE
160 170 180 190 200
TVGQSLLQQT QMGDIWPGRR IIQEQQKEQS VLFEVPKLKS GKSALEAESE
210 220 230 240 250
SLDSYTADSD STSRRDSLDK SGLFPEWKKM SAPKSQVEKE IAPGNQNAVC
260 270 280 290 300
GDEGDMIFKK NTRKVLRPSE YTKSVIDLRP EDVAQESGIL GDRSKSVPGL
310 320 330 340 350
SVDMEDEEEE EEDIDHLVKL HRQKLARGSM QSGSSMSTIG SMMSLYSEAG
360 370 380 390 400
DFGNVSVTGK IAFSLKFEQK TQTLVIHVKE CHQLAYADEA KKRSNPYVKT
410 420 430 440 450
YLLPDKSRQG KRKTSIKRDT INPLYDETFR YEISESLLAQ RTLQFSVWHH
460 470 480 490 500
GRFGRNTFLG EAEVHMDSWK LDKKLDHCLP LHGKISTESS PGLPAHKGEL
510 520 530 540 550
VVSLKYIPAS KLPVGGDRKK SKGGEGGELQ VWIKEAKNLT AAKSGGTSDS
560 570 580 590 600
FVKGYLLPMR NKASKRKTPV MKKTLNPHYN HTFVYNGVRL EDLQHMCLEL
610 620 630 640 650
TVWDREPLAS NDFLGGVRLG VGTGISSGEV VDWMDSTGEE VSLWQKMRQY
660 670
PGSWAEGTLQ LRSSMVKQKL GV
Length:672
Mass (Da):75,900
Last modified:March 1, 2002 - v1
Checksum:i0F994F4D85202400
GO
Isoform 2 (identifier: Q8VHQ7-2) [UniParc]FASTAAdd to Basket

Also known as: Granuphilin-b

The sequence of this isoform differs from the canonical sequence as follows:
     485-501: ISTESSPGLPAHKGELV → GSVVAKWWTGWIRLVKK
     502-672: Missing.

Show »
Length:501
Mass (Da):57,326
Checksum:iB2F11B652A96AE27
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei485 – 50117ISTES…KGELV → GSVVAKWWTGWIRLVKK in isoform 2. 1 PublicationVSP_007903Add
BLAST
Alternative sequencei502 – 672171Missing in isoform 2. 1 PublicationVSP_007904Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419341 mRNA. Translation: AAL38513.1.
AF419342 mRNA. Translation: AAL38514.1.
RefSeqiNP_536335.1. NM_080410.1. [Q8VHQ7-1]
UniGeneiRn.107417.

Genome annotation databases

GeneIDi140594.
KEGGirno:140594.
UCSCiRGD:620204. rat. [Q8VHQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419341 mRNA. Translation: AAL38513.1 .
AF419342 mRNA. Translation: AAL38514.1 .
RefSeqi NP_536335.1. NM_080410.1. [Q8VHQ7-1 ]
UniGenei Rn.107417.

3D structure databases

ProteinModelPortali Q8VHQ7.
SMRi Q8VHQ7. Positions 43-106.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250808. 1 interaction.
IntActi Q8VHQ7. 1 interaction.

Proteomic databases

PaxDbi Q8VHQ7.
PRIDEi Q8VHQ7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 140594.
KEGGi rno:140594.
UCSCi RGD:620204. rat. [Q8VHQ7-1 ]

Organism-specific databases

CTDi 94121.
RGDi 620204. Sytl4.

Phylogenomic databases

eggNOGi NOG293068.
HOGENOMi HOG000231332.
HOVERGENi HBG054255.
InParanoidi Q8VHQ7.
KOi K17598.
PhylomeDBi Q8VHQ7.

Miscellaneous databases

NextBioi 620570.
PROi Q8VHQ7.

Gene expression databases

Genevestigatori Q8VHQ7.

Family and domain databases

Gene3Di 2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR000008. C2_dom.
IPR028694. SYTL4.
IPR010911. Znf_FYVE-typ.
IPR011011. Znf_FYVE_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
PANTHERi PTHR10024:SF9. PTHR10024:SF9. 1 hit.
Pfami PF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 2 hits.
SM00184. RING. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEi PS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Pancreatic beta-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosis."
    Coppola T., Frantz C., Perret-Menoud V., Gattesco S., Hirling H., Regazzi R.
    Mol. Biol. Cell 13:1906-1915(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF VAL-21 AND LEU-43, INTERACTION WITH RAB3A AND STXBP1, SUBCELLULAR LOCATION.
    Tissue: Insulinoma.

Entry informationi

Entry nameiSYTL4_RAT
AccessioniPrimary (citable) accession number: Q8VHQ7
Secondary accession number(s): Q8VHQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3