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Protein

Protein phosphatase 1 regulatory inhibitor subunit 16B

Gene

Ppp1r16b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (PubMed:21907835). Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory inhibitor subunit 16B
Alternative name(s):
CAAX box protein TIMAP
TGF-beta-inhibited membrane-associated protein
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2151841. Ppp1r16b.

Subcellular locationi

  • Cell membrane By similarity
  • Cell membrane Curated; Lipid-anchor Curated
  • Nucleus By similarity
  • Cell projection By similarity

  • Note: Colocalizes with RPSA/LAMR1 in the cell membrane (By similarity). Localizes to the perinuclear region (By similarity). Colocalizes with PTEN at the tip of EC projections (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670441 – 565Protein phosphatase 1 regulatory inhibitor subunit 16BAdd BLAST565
PropeptideiPRO_0000396711566 – 568Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphoserineCombined sources1
Modified residuei350PhosphoserineBy similarity1
Modified residuei477PhosphoserineBy similarity1
Lipidationi564S-palmitoyl cysteineBy similarity1
Modified residuei565Cysteine methyl esterBy similarity1
Lipidationi565S-farnesyl cysteineBy similarity1

Post-translational modificationi

Phosphorylated by PKA and, after PKA priming, by GSK3B. Phosphorylation by GSK3B reduces its association with PP1C and enhances PP1C activity. Dephosphorylation by its associated PP1C results in enhanced association with PP1C, but reduced PP1C activity (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ8VHQ3.
PaxDbiQ8VHQ3.
PRIDEiQ8VHQ3.

PTM databases

iPTMnetiQ8VHQ3.
PhosphoSitePlusiQ8VHQ3.

Expressioni

Inductioni

Inhibited by TGF-beta1 (Probable). Down-regulated by LPS (PubMed:21907835).Curated1 Publication

Gene expression databases

BgeeiENSMUSG00000037754.
ExpressionAtlasiQ8VHQ3. baseline and differential.
GenevisibleiQ8VHQ3. MM.

Interactioni

Subunit structurei

Interacts with PPP1CA, PPP1CB and MSN. Interacts (via its fourth ankyrin repeat) with the mature dimeric form of RPSA/LAMR1 (By similarity). Interacts with EEF1A1 (By similarity). Interacts with PTEN (By similarity). Interacts with ECE1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039540.

Structurei

3D structure databases

ProteinModelPortaliQ8VHQ3.
SMRiQ8VHQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati100 – 129ANK 1Add BLAST30
Repeati133 – 162ANK 2Add BLAST30
Repeati228 – 257ANK 3Add BLAST30
Repeati261 – 290ANK 4Add BLAST30
Repeati531 – 560ANK 5Add BLAST30

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili15 – 55Sequence analysisAdd BLAST41

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ8VHQ3.
KOiK17459.
OMAiATCGHIN.
OrthoDBiEOG091G06S2.
PhylomeDBiQ8VHQ3.
TreeFamiTF316803.

Family and domain databases

CDDicd00204. ANK. 2 hits.
Gene3Di1.25.40.20. 2 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 5 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VHQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHVDLLTE LQLLEKVPTL ERLRAAQKRR AQQLKKWAQY EQDLLHRKRK
60 70 80 90 100
HERKRSTGGR RKKVSFEASV ALLEASLRND AEEVRYFLKN KVSPDLCNED
110 120 130 140 150
GLTALHQCCI DNFEEIVKLL LSHGANVNAK DNELWTPLHA AATCGHINLV
160 170 180 190 200
KILVQYGADL LAVNSDGNMP YDLCEDEPTL DVIETCMAYQ GITQEKINEM
210 220 230 240 250
RAAPEQKMIS DIHCMIAAGQ DLDWIDGQGA TLLHIAGANG YLRAAELLLD
260 270 280 290 300
HGVRVDVKDW DGWEPLHAAA FWGQMPMAEL LVSHGASLSA RTSMDEMPID
310 320 330 340 350
LCEEEEFKVL LLELKHKHDV IMKSQLRHKS SLSRRTSSAG SRGKVVRRAS
360 370 380 390 400
LSDRTNLYRK EYEGEAILWQ QRSAAEDQRT STYNGDIRET RTDQENKDPN
410 420 430 440 450
PRLEKPVLLS EFSTKISRGE LDGPVENGLR APVSTYQYAL ANGDIWKMHE
460 470 480 490 500
MPDYSMAYGN PGVADVPPPW SGFKEQSPQT LLELKRQRAA AKLLSHPFLS
510 520 530 540 550
THLGSSVARS GESSSEGKAP LIGGRTSPYS SNGTSVYYTV TSGDPPLLKF
560
KAPMEEMEEK VHGCCRIS
Length:568
Mass (Da):63,571
Last modified:March 1, 2002 - v1
Checksum:i5D8446C81DEBA3D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF423761 mRNA. Translation: AAL62093.1.
BC054764 mRNA. Translation: AAH54764.1.
BC057542 mRNA. Translation: AAH57542.1.
CCDSiCCDS16991.1.
RefSeqiNP_001153134.1. NM_001159662.1.
NP_694729.1. NM_153089.4.
XP_011237794.1. XM_011239492.2.
UniGeneiMm.150540.
Mm.491639.

Genome annotation databases

EnsembliENSMUST00000045503; ENSMUSP00000039540; ENSMUSG00000037754.
ENSMUST00000052927; ENSMUSP00000062615; ENSMUSG00000037754.
ENSMUST00000103116; ENSMUSP00000099405; ENSMUSG00000037754.
GeneIDi228852.
KEGGimmu:228852.
UCSCiuc008nqo.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiPP16B_MOUSE
AccessioniPrimary (citable) accession number: Q8VHQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2002
Last modified: August 30, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot