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Protein

Protein phosphatase 1 regulatory inhibitor subunit 16B

Gene

Ppp1r16b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory inhibitor subunit 16B
Alternative name(s):
CAAX box protein TIMAP
TGF-beta-inhibited membrane-associated protein
Gene namesi
Name:Ppp1r16b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2151841. Ppp1r16b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Protein phosphatase 1 regulatory inhibitor subunit 16BPRO_0000067044Add
BLAST
Propeptidei566 – 5683Removed in mature formSequence analysisPRO_0000396711

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei69 – 691PhosphoserineCombined sources
Modified residuei350 – 3501PhosphoserineBy similarity
Modified residuei477 – 4771PhosphoserineBy similarity
Lipidationi564 – 5641S-palmitoyl cysteineBy similarity
Modified residuei565 – 5651Cysteine methyl esterBy similarity
Lipidationi565 – 5651S-farnesyl cysteineBy similarity

Post-translational modificationi

Phosphorylated by PKA and, after PKA priming, by GSK3B. Phosphorylation by GSK3B reduces its association with PP1C and enhances PP1C activity. Dephosphorylation by its associated PP1C results in enhanced association with PP1C, but reduced PP1C activity (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ8VHQ3.
PaxDbiQ8VHQ3.
PRIDEiQ8VHQ3.

PTM databases

iPTMnetiQ8VHQ3.
PhosphoSiteiQ8VHQ3.

Expressioni

Gene expression databases

BgeeiQ8VHQ3.
ExpressionAtlasiQ8VHQ3. baseline and differential.
GenevisibleiQ8VHQ3. MM.

Interactioni

Subunit structurei

Interacts with PPP1CA, PPP1CB and MSN. Interacts (via its fourth ankyrin repeat) with the mature dimeric form of RPSA/LAMR1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039540.

Structurei

3D structure databases

ProteinModelPortaliQ8VHQ3.
SMRiQ8VHQ3. Positions 22-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati100 – 12930ANK 1Add
BLAST
Repeati133 – 16230ANK 2Add
BLAST
Repeati228 – 25730ANK 3Add
BLAST
Repeati261 – 29030ANK 4Add
BLAST
Repeati531 – 56030ANK 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili15 – 5541Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ8VHQ3.
KOiK17459.
OMAiNAKDNEL.
OrthoDBiEOG7P5T0H.
PhylomeDBiQ8VHQ3.
TreeFamiTF316803.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VHQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHVDLLTE LQLLEKVPTL ERLRAAQKRR AQQLKKWAQY EQDLLHRKRK
60 70 80 90 100
HERKRSTGGR RKKVSFEASV ALLEASLRND AEEVRYFLKN KVSPDLCNED
110 120 130 140 150
GLTALHQCCI DNFEEIVKLL LSHGANVNAK DNELWTPLHA AATCGHINLV
160 170 180 190 200
KILVQYGADL LAVNSDGNMP YDLCEDEPTL DVIETCMAYQ GITQEKINEM
210 220 230 240 250
RAAPEQKMIS DIHCMIAAGQ DLDWIDGQGA TLLHIAGANG YLRAAELLLD
260 270 280 290 300
HGVRVDVKDW DGWEPLHAAA FWGQMPMAEL LVSHGASLSA RTSMDEMPID
310 320 330 340 350
LCEEEEFKVL LLELKHKHDV IMKSQLRHKS SLSRRTSSAG SRGKVVRRAS
360 370 380 390 400
LSDRTNLYRK EYEGEAILWQ QRSAAEDQRT STYNGDIRET RTDQENKDPN
410 420 430 440 450
PRLEKPVLLS EFSTKISRGE LDGPVENGLR APVSTYQYAL ANGDIWKMHE
460 470 480 490 500
MPDYSMAYGN PGVADVPPPW SGFKEQSPQT LLELKRQRAA AKLLSHPFLS
510 520 530 540 550
THLGSSVARS GESSSEGKAP LIGGRTSPYS SNGTSVYYTV TSGDPPLLKF
560
KAPMEEMEEK VHGCCRIS
Length:568
Mass (Da):63,571
Last modified:March 1, 2002 - v1
Checksum:i5D8446C81DEBA3D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF423761 mRNA. Translation: AAL62093.1.
BC054764 mRNA. Translation: AAH54764.1.
BC057542 mRNA. Translation: AAH57542.1.
CCDSiCCDS16991.1.
RefSeqiNP_001153134.1. NM_001159662.1.
NP_694729.1. NM_153089.4.
XP_011237794.1. XM_011239492.1.
UniGeneiMm.150540.
Mm.491639.

Genome annotation databases

EnsembliENSMUST00000045503; ENSMUSP00000039540; ENSMUSG00000037754.
ENSMUST00000052927; ENSMUSP00000062615; ENSMUSG00000037754.
ENSMUST00000103116; ENSMUSP00000099405; ENSMUSG00000037754.
GeneIDi228852.
KEGGimmu:228852.
UCSCiuc008nqo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF423761 mRNA. Translation: AAL62093.1.
BC054764 mRNA. Translation: AAH54764.1.
BC057542 mRNA. Translation: AAH57542.1.
CCDSiCCDS16991.1.
RefSeqiNP_001153134.1. NM_001159662.1.
NP_694729.1. NM_153089.4.
XP_011237794.1. XM_011239492.1.
UniGeneiMm.150540.
Mm.491639.

3D structure databases

ProteinModelPortaliQ8VHQ3.
SMRiQ8VHQ3. Positions 22-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039540.

PTM databases

iPTMnetiQ8VHQ3.
PhosphoSiteiQ8VHQ3.

Proteomic databases

MaxQBiQ8VHQ3.
PaxDbiQ8VHQ3.
PRIDEiQ8VHQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045503; ENSMUSP00000039540; ENSMUSG00000037754.
ENSMUST00000052927; ENSMUSP00000062615; ENSMUSG00000037754.
ENSMUST00000103116; ENSMUSP00000099405; ENSMUSG00000037754.
GeneIDi228852.
KEGGimmu:228852.
UCSCiuc008nqo.2. mouse.

Organism-specific databases

CTDi26051.
MGIiMGI:2151841. Ppp1r16b.

Phylogenomic databases

eggNOGiKOG0505. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000236276.
HOVERGENiHBG053644.
InParanoidiQ8VHQ3.
KOiK17459.
OMAiNAKDNEL.
OrthoDBiEOG7P5T0H.
PhylomeDBiQ8VHQ3.
TreeFamiTF316803.

Miscellaneous databases

ChiTaRSiPpp1r16b. mouse.
PROiQ8VHQ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VHQ3.
ExpressionAtlasiQ8VHQ3. baseline and differential.
GenevisibleiQ8VHQ3. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017417. Pase-1_reg_su_16AB.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038159. PP1_16AB_vert. 1 hit.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "mPPP1R16B is a novel mouse protein phosphatase 1 targeting subunit whose mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain."
    Magdaleno S., Northcutt G.M., Curran T., Kurschner C.
    Gene Expr. Patterns 1:143-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.

Entry informationi

Entry nameiPP16B_MOUSE
AccessioniPrimary (citable) accession number: Q8VHQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Repressed by TGF-beta1 which requires denovo protein synthesis and histone deacetylase.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.