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Q8VHL1 (SETD7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase SETD7

EC=2.1.1.43
Alternative name(s):
Histone H3-K4 methyltransferase SETD7
Short name=H3-K4-HMTase SETD7
SET domain-containing protein 7
SET7/9
Gene names
Name:Setd7
Synonyms:Kiaa1717, Set7, Set9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Ref.5

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subunit structure

Interacts with IPF1/PDX-1 By similarity.

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Developmental stage

Expressed during all pre-implementation stages in both male and female embryos. Ref.6

Domain

The SET domain is necessary but not sufficient for histone methyltransferase activity By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.

Contains 3 MORN repeats.

Contains 1 SET domain.

Sequence caution

The sequence BAC98238.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Histone-lysine N-methyltransferase SETD7
PRO_0000186055

Regions

Repeat36 – 5823MORN 1
Repeat59 – 8123MORN 2
Repeat106 – 12823MORN 3
Domain214 – 336123SET
Region226 – 2283S-adenosyl-L-methionine binding By similarity
Region256 – 2583Substrate binding By similarity
Region266 – 2683Substrate binding By similarity
Region296 – 2972S-adenosyl-L-methionine binding By similarity
Compositional bias51 – 544Poly-Phe

Sites

Binding site2451Substrate By similarity
Binding site3171Substrate By similarity
Binding site3351Substrate; via carbonyl oxygen By similarity
Binding site3561S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Experimental info

Sequence conflict771E → V in AAH50190. Ref.4
Sequence conflict1651L → I in AAL56578. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8VHL1 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: C826EAFCB4B9D345

FASTA36640,506
        10         20         30         40         50         60 
MDSDDEVVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE 

        70         80         90        100        110        120 
GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDS DGRLIFKGQY KDNNRHGVCW 

       130        140        150        160        170        180 
IHYPDGGSLV GEVNEDGEMT GEKIAYVYPD QRTALYGKFI DGEMLEGKLA TLMATEEGRP 

       190        200        210        220        230        240 
HFEVTSGSSV YHFDKSTSSC ISSDALLPDP YESERVYVAD SLISSAGEGL FSKVAVGPNT 

       250        260        270        280        290        300 
VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT 

       310        320        330        340        350        360 
PNCVYDLFVH PRFGPIKCIR TLRAVEAEEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF 


QATQQK 

« Hide

References

« Hide 'large scale' references
[1]"Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase."
Wang H., Cao R., Xia L., Erdjument-Bromage H., Borchers C., Tempst P., Zhang Y.
Mol. Cell 8:1207-1217(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreas.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryonic tail.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and NOD.
Tissue: Brain and Cerebellum.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: 129/Sv X 129SvCp.
Tissue: Embryonic stem cell.
[5]"Covalent histone modifications underlie the developmental regulation of insulin gene transcription in pancreatic beta cells."
Chakrabarti S.K., Francis J., Ziesmann S.M., Garmey J.C., Mirmira R.G.
J. Biol. Chem. 278:23617-23623(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The analysis of X-chromosome inactivation-related gene expression from single mouse embryo with sex-determination."
Jeong K.S., Park J.H., Lee S.
Biochem. Biophys. Res. Commun. 333:803-807(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF448509 mRNA. Translation: AAL56578.1.
AK129428 mRNA. Translation: BAC98238.1. Different initiation.
AK048924 mRNA. Translation: BAC33493.1.
AK147422 mRNA. Translation: BAE27903.1.
AK147413 mRNA. Translation: BAE27897.1.
AK147667 mRNA. Translation: BAE28059.1.
AK170161 mRNA. Translation: BAE41607.1.
BC050190 mRNA. Translation: AAH50190.1.
RefSeqNP_542983.3. NM_080793.5.
UniGeneMm.192111.

3D structure databases

ProteinModelPortalQ8VHL1.
SMRQ8VHL1. Positions 11-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid215865. 1 interaction.
IntActQ8VHL1. 1 interaction.
MINTMINT-4114741.

PTM databases

PhosphoSiteQ8VHL1.

Proteomic databases

PaxDbQ8VHL1.
PRIDEQ8VHL1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037141; ENSMUSP00000043492; ENSMUSG00000037111.
GeneID73251.
KEGGmmu:73251.
UCSCuc008peb.1. mouse.

Organism-specific databases

CTD80854.
MGIMGI:1920501. Setd7.
RougeSearch...

Phylogenomic databases

eggNOGCOG4642.
GeneTreeENSGT00390000004827.
HOGENOMHOG000074731.
HOVERGENHBG028309.
InParanoidQ8VHL1.
KOK11431.
OMAGSSVYHF.
OrthoDBEOG7M98G8.
PhylomeDBQ8VHL1.
TreeFamTF106392.

Gene expression databases

BgeeQ8VHL1.
GenevestigatorQ8VHL1.

Family and domain databases

InterProIPR017155. Hist-Lys_N-MeTrfase_SET.
IPR003409. MORN.
IPR001214. SET_dom.
[Graphical view]
PfamPF02493. MORN. 4 hits.
PF00856. SET. 1 hit.
[Graphical view]
PIRSFPIRSF037249. Histone_Lys_mtfrase_SET. 1 hit.
SMARTSM00317. SET. 1 hit.
[Graphical view]
PROSITEPS51577. SAM_MT43_SET7. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSETD7. mouse.
NextBio337766.
PROQ8VHL1.
SOURCESearch...

Entry information

Entry nameSETD7_MOUSE
AccessionPrimary (citable) accession number: Q8VHL1
Secondary accession number(s): Q6ZPJ6, Q80UU3, Q8C7Y6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot