Q8VHL0 (UT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urea transporter 1

Alternative name(s):
Solute carrier family 14 member 1
Urea transporter B
Short name=UT-B
Urea transporter, erythrocyte

Gene names

Name:

Slc14a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-affinity facilitative urea transporter that allow rapid equilibration between the urinary space and the hyperosmotic interstitium. the rate of urea conduction is increased by hypoosmotic stress By similarity. Mediates urea transport in erythrocytes. Ref.1
Subunit structure	Homotrimer By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. Note: Restricted to the basolateral membrane in various portions of the urothelium. Ref.2
Tissue specificity	Expressed in brain, kidney, heart, liver, lung, skeletal muscle, spleen, testis, ureter and urinary bladder (at protein level). Along the gastrointestinal tract, detected in colon, jejunum and stomach (at protein level). In the kidney, expressed in some microvessels of the inner and outer medulla, but not all (at protein level). Not detected in the cortex (at protein level). Detected in the urothelium all along the urinary tract, including the papilla surface, the ureter, the bladder and the urethra (at protein level). In the brain, expressed at the border of the corpus callosum and striatum in astrocytic cellular processes surrounding blood microvessels (at protein level). Ref.1 Ref.2
Induction	Down-regulated by water deprivation in urinary bladder and ureter, but not in kidney medulla, colon, testis nor brain. Ref.2
Post-translational modification	N-glycosylated in red blood cells, as well as in most non-erythroid tissues, except in the gastrocnemius muscle and in the gastrointestinal tract, including liver, colon and stomach. Ref.2
Disruption phenotype	Mutant mice exhibit grossly normal appearance, activity and behavior. Plasma sodium, potassium, chloride, bicarbonate and creatinine concentrations, as well as hematocrit, are similar to wild type animals. Urea permeability in erythrocytes is 45-fold lower than that from wild-type mice. Daily urine output was 1.5-fold greater and urine osmolarity was lower than in wild-type mice. After 24 hours of water deprivation, plasma urea concentration is 30% higher and urine urea concentration 35% lower in mutant mice than in wild-type animals. Ref.1

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement Ref.1. Source: MGI plasma membrane Inferred from direct assay PubMed 12133842. Source: MGI
Molecular_function	urea transmembrane transporter activity Inferred from mutant phenotype Ref.1. Source: MGI water transmembrane transporter activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VHL0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VHL0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNGQSLTGGTDDAHHGPLWIDPFGNRGDKAAPEGFRRLSLALAQRWREQEPEEEIAM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Urea transporter 1		PRO_0000065738
Regions
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Transmembrane	250 – 270	21	Helical; Potential
	Transmembrane	276 – 296	21	Helical; Potential
	Transmembrane	305 – 325	21	Helical; Potential
	Transmembrane	327 – 347	21	Helical; Potential
Amino acid modifications
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1	1	M → MNGQSLTGGTDDAHHGPLWI DPFGNRGDKAAPEGFRRLSL ALAQRWREQEPEEEIAM in isoform 2.		VSP_041574
Experimental info
	Sequence conflict	8	1	V → A in AAL47138. Ref.1
	Sequence conflict	50	1	V → A in AAI00571. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: E66ED087341C07B7	FASTA	384	42,126
	10 20 30 40 50 60 MEDSPTMVKV DRGENQILSC RGRRCGFKVL GYVTGDMKEF ANWLKDKPVV LQFMDWILRG 70 80 90 100 110 120 ISQVVFVSNP ISGILILVGL LVQNPWWALC GCVGTVVSTL TALLLSQDRS AIAAGLQGYN 130 140 150 160 170 180 ATLVGILMAV FSNKGDYFWW LIFPVSAMSM TCPVFSSALS SVLSKWDLPV FTLPFNMALS 190 200 210 220 230 240 MYLSATGHYN TFFPSKLFTP VSSVPNITWS ELSALELLKS LPVGVGQIYG CDNPWTGGIF 250 260 270 280 290 300 LCAILLSSPL MCLHAAIGSL LGVIAGLSLA APFEDIYFGL WGFNSSLACI AIGGMFMALT 310 320 330 340 350 360 WQTHLLALAC ALFTAYFGAC MAHLMAVVHL PACTWSFCLA TLLFLLLTTK NPNIYRMPLS 370 380 KVTYSEENRI FYLQNKKRMV ESPL
	Isoform 2 [UniParc]. Checksum: BA248DED75D9772A	FASTA	440	48,338
	10 20 30 40 50 60 MNGQSLTGGT DDAHHGPLWI DPFGNRGDKA APEGFRRLSL ALAQRWREQE PEEEIAMEDS 70 80 90 100 110 120 PTMVKVDRGE NQILSCRGRR CGFKVLGYVT GDMKEFANWL KDKPVVLQFM DWILRGISQV 130 140 150 160 170 180 VFVSNPISGI LILVGLLVQN PWWALCGCVG TVVSTLTALL LSQDRSAIAA GLQGYNATLV 190 200 210 220 230 240 GILMAVFSNK GDYFWWLIFP VSAMSMTCPV FSSALSSVLS KWDLPVFTLP FNMALSMYLS 250 260 270 280 290 300 ATGHYNTFFP SKLFTPVSSV PNITWSELSA LELLKSLPVG VGQIYGCDNP WTGGIFLCAI 310 320 330 340 350 360 LLSSPLMCLH AAIGSLLGVI AGLSLAAPFE DIYFGLWGFN SSLACIAIGG MFMALTWQTH 370 380 390 400 410 420 LLALACALFT AYFGACMAHL MAVVHLPACT WSFCLATLLF LLLTTKNPNI YRMPLSKVTY 430 440 SEENRIFYLQ NKKRMVESPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Urea-selective concentrating defect in transgenic mice lacking urea transporter UT-B." Yang B., Bankir L., Gillespie A., Epstein C.J., Verkman A.S. J. Biol. Chem. 277:10633-10637(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: C57BL/6. Tissue: Kidney.
[2]	"UT-B1 urea transporter is expressed along the urinary and gastrointestinal tracts of the mouse." Lucien N., Bruneval P., Lasbennes F., Belair M.F., Mandet C., Cartron J.P., Bailly P., Trinh-Trang-Tan M.M. Am. J. Physiol. 288:R1046-R1056(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, INDUCTION. Strain: BALB/c. Tissue: Kidney.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Embryo and Thymus.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6 and C57BL/6NCr. Tissue: Eye, Head and Hematopoietic stem cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF448798 mRNA. Translation: AAL47138.1. AJ420967 mRNA. Translation: CAD12807.1. AK012066 mRNA. Translation: BAB28004.1. AK041979 mRNA. Translation: BAC31119.1. AK153891 mRNA. Translation: BAE32238.1. CH466528 Genomic DNA. Translation: EDL09437.1. CH466528 Genomic DNA. Translation: EDL09438.1. BC058594 mRNA. Translation: AAH58594.2. BC086673 mRNA. Translation: AAH86673.1. BC100570 mRNA. Translation: AAI00571.2.
IPI	IPI00420812. IPI00652728.
RefSeq	NP_001164481.1. NM_001171010.1. NP_001164482.1. NM_001171011.1. NP_082398.1. NM_028122.4.
UniGene	Mm.33832.
3D structure databases
ProteinModelPortal	Q8VHL0.
SMR	Q8VHL0. Positions 31-376.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000025433.
Proteomic databases
PaxDb	Q8VHL0.
PRIDE	Q8VHL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000091813; ENSMUSP00000089421; ENSMUSG00000059336. ENSMUST00000160292; ENSMUSP00000125114; ENSMUSG00000059336. ENSMUST00000160639; ENSMUSP00000125367; ENSMUSG00000059336.
GeneID	108052.
KEGG	mmu:108052.
Organism-specific databases
CTD	6563.
MGI	MGI:1351654. Slc14a1.
Phylogenomic databases
eggNOG	COG4413.
GeneTree	ENSGT00390000018729.
HOGENOM	HOG000065705.
HOVERGEN	HBG000540.
InParanoid	Q3U542.
KO	K08716.
OMA	NRIFYLQ.
OrthoDB	EOG48D0VG.
Gene expression databases
Bgee	Q8VHL0.
Genevestigator	Q8VHL0.
GermOnline	ENSMUSG00000059336. Mus musculus.
Family and domain databases
InterPro	IPR004937. Urea_transporter. [Graphical view]
PANTHER	PTHR10464. PTHR10464. 1 hit.
Pfam	PF03253. UT. 1 hit. [Graphical view]
PIRSF	PIRSF016502. Urea_transporter. 1 hit.
ProtoNet	Search...
Other
NextBio	359947.
SOURCE	Search...

Entry information

Entry name

UT1_MOUSE

Accession

Primary (citable) accession number: Q8VHL0
Secondary accession number(s): Q3U542 , Q497G1, Q5RJG2, Q6PDP4, Q9CZX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program