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Q8VHL0

- UT1_MOUSE

UniProt

Q8VHL0 - UT1_MOUSE

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Protein

Urea transporter 1

Gene
Slc14a1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei334 – 3341Important for channel permeability By similarity

GO - Molecular functioni

  1. urea channel activity Source: UniProtKB
  2. urea transmembrane transporter activity Source: MGI
  3. water transmembrane transporter activity Source: MGI

GO - Biological processi

  1. urea transmembrane transport Source: UniProtKB
  2. urea transport Source: MGI
  3. water transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Names & Taxonomyi

Protein namesi
Recommended name:
Urea transporter 1
Alternative name(s):
Solute carrier family 14 member 1
Urea transporter B
Short name:
UT-B
Urea transporter, erythrocyte
Gene namesi
Name:Slc14a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1351654. Slc14a1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein
Note: Restricted to the basolateral membrane in various portions of the urothelium.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei47 – 6418Helical; By similarityAdd
BLAST
Intramembranei68 – 8316Helical; By similarityAdd
BLAST
Transmembranei86 – 10520Helical; By similarityAdd
BLAST
Transmembranei110 – 13223Helical; By similarityAdd
BLAST
Transmembranei139 – 16325Helical; By similarityAdd
BLAST
Transmembranei168 – 18821Helical; By similarityAdd
BLAST
Intramembranei215 – 22915Helical; By similarityAdd
BLAST
Intramembranei234 – 24512Helical; By similarityAdd
BLAST
Transmembranei250 – 26920Helical; By similarityAdd
BLAST
Transmembranei279 – 29921Helical; By similarityAdd
BLAST
Transmembranei304 – 32522Helical; By similarityAdd
BLAST
Transmembranei328 – 34821Helical; By similarityAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: MGI
  3. integral component of plasma membrane Source: UniProtKB
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice exhibit grossly normal appearance, activity and behavior. Plasma sodium, potassium, chloride, bicarbonate and creatinine concentrations, as well as hematocrit, are similar to wild type animals. Urea permeability in erythrocytes is 45-fold lower than that from wild-type mice. Daily urine output is 1.5-fold greater and urine osmolarity is lower than in wild-type mice. After 24 hours of water deprivation, plasma urea concentration is 30% higher and urine urea concentration 35% lower in mutant mice than in wild-type animals. Mice lacking both Aqp1 and Slc14a1 are born at the expected Mendelian ratio, but do not thrive; half of them die within ten days after birth and none are alive after two weeks. Urine osmolality is somewhat lower than that observed with mice lacking Aqp1. Besides, erythrocyte water permeability is significantly lower than in mice lacking only Aqp1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Urea transporter 1PRO_0000065738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi206 – 2061N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated in red blood cells, as well as in most non-erythroid tissues, except in the gastrocnemius muscle and in the gastrointestinal tract, including liver, colon and stomach.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8VHL0.
PaxDbiQ8VHL0.
PRIDEiQ8VHL0.

Expressioni

Tissue specificityi

Expressed in brain, kidney, heart, liver, lung, skeletal muscle, spleen, testis, ureter and urinary bladder (at protein level). Along the gastrointestinal tract, detected in colon, jejunum and stomach (at protein level). In the kidney, expressed in some microvessels of the inner and outer medulla, but not all (at protein level). Not detected in the cortex (at protein level). Detected in the urothelium all along the urinary tract, including the papilla surface, the ureter, the bladder and the urethra (at protein level). In the brain, expressed at the border of the corpus callosum and striatum in astrocytic cellular processes surrounding blood microvessels (at protein level). Detected in erythrocytes (at protein level).2 Publications

Inductioni

Down-regulated by water deprivation in urinary bladder and ureter, but not in kidney medulla, colon, testis nor brain.1 Publication

Gene expression databases

BgeeiQ8VHL0.
GenevestigatoriQ8VHL0.

Interactioni

Subunit structurei

Homotrimer; each subunit contains a pore through which urea permeates. Identified in a complex with STOM By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025433.

Structurei

3D structure databases

ProteinModelPortaliQ8VHL0.
SMRiQ8VHL0. Positions 31-376.

Family & Domainsi

Sequence similaritiesi

Belongs to the urea transporter family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4413.
GeneTreeiENSGT00390000018729.
HOGENOMiHOG000065705.
HOVERGENiHBG000540.
InParanoidiQ3U542.
KOiK08716.
OMAiPNIYKMP.
OrthoDBiEOG7GBFX8.
TreeFamiTF332858.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR004937. Urea_transporter.
[Graphical view]
PANTHERiPTHR10464. PTHR10464. 1 hit.
PfamiPF03253. UT. 1 hit.
[Graphical view]
PIRSFiPIRSF016502. Urea_transporter. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VHL0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEDSPTMVKV DRGENQILSC RGRRCGFKVL GYVTGDMKEF ANWLKDKPVV    50
LQFMDWILRG ISQVVFVSNP ISGILILVGL LVQNPWWALC GCVGTVVSTL 100
TALLLSQDRS AIAAGLQGYN ATLVGILMAV FSNKGDYFWW LIFPVSAMSM 150
TCPVFSSALS SVLSKWDLPV FTLPFNMALS MYLSATGHYN TFFPSKLFTP 200
VSSVPNITWS ELSALELLKS LPVGVGQIYG CDNPWTGGIF LCAILLSSPL 250
MCLHAAIGSL LGVIAGLSLA APFEDIYFGL WGFNSSLACI AIGGMFMALT 300
WQTHLLALAC ALFTAYFGAC MAHLMAVVHL PACTWSFCLA TLLFLLLTTK 350
NPNIYRMPLS KVTYSEENRI FYLQNKKRMV ESPL 384
Length:384
Mass (Da):42,126
Last modified:June 28, 2011 - v2
Checksum:iE66ED087341C07B7
GO
Isoform 2 (identifier: Q8VHL0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNGQSLTGGTDDAHHGPLWIDPFGNRGDKAAPEGFRRLSLALAQRWREQEPEEEIAM

Show »
Length:440
Mass (Da):48,338
Checksum:iBA248DED75D9772A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MNGQSLTGGTDDAHHGPLWI DPFGNRGDKAAPEGFRRLSL ALAQRWREQEPEEEIAM in isoform 2. VSP_041574

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81V → A in AAL47138. 1 Publication
Sequence conflicti50 – 501V → A in AAI00571. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448798 mRNA. Translation: AAL47138.1.
AJ420967 mRNA. Translation: CAD12807.1.
AK012066 mRNA. Translation: BAB28004.1.
AK041979 mRNA. Translation: BAC31119.1.
AK153891 mRNA. Translation: BAE32238.1.
CH466528 Genomic DNA. Translation: EDL09437.1.
CH466528 Genomic DNA. Translation: EDL09438.1.
BC058594 mRNA. Translation: AAH58594.2.
BC086673 mRNA. Translation: AAH86673.1.
BC100570 mRNA. Translation: AAI00571.2.
CCDSiCCDS29360.1. [Q8VHL0-1]
CCDS50330.1. [Q8VHL0-2]
RefSeqiNP_001164481.1. NM_001171010.1. [Q8VHL0-2]
NP_001164482.1. NM_001171011.1. [Q8VHL0-1]
NP_082398.1. NM_028122.4. [Q8VHL0-1]
UniGeneiMm.33832.

Genome annotation databases

EnsembliENSMUST00000091813; ENSMUSP00000089421; ENSMUSG00000059336. [Q8VHL0-1]
ENSMUST00000160292; ENSMUSP00000125114; ENSMUSG00000059336. [Q8VHL0-2]
ENSMUST00000160639; ENSMUSP00000125367; ENSMUSG00000059336. [Q8VHL0-1]
GeneIDi108052.
KEGGimmu:108052.
UCSCiuc008fsc.2. mouse. [Q8VHL0-1]
uc008fsd.2. mouse. [Q8VHL0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448798 mRNA. Translation: AAL47138.1 .
AJ420967 mRNA. Translation: CAD12807.1 .
AK012066 mRNA. Translation: BAB28004.1 .
AK041979 mRNA. Translation: BAC31119.1 .
AK153891 mRNA. Translation: BAE32238.1 .
CH466528 Genomic DNA. Translation: EDL09437.1 .
CH466528 Genomic DNA. Translation: EDL09438.1 .
BC058594 mRNA. Translation: AAH58594.2 .
BC086673 mRNA. Translation: AAH86673.1 .
BC100570 mRNA. Translation: AAI00571.2 .
CCDSi CCDS29360.1. [Q8VHL0-1 ]
CCDS50330.1. [Q8VHL0-2 ]
RefSeqi NP_001164481.1. NM_001171010.1. [Q8VHL0-2 ]
NP_001164482.1. NM_001171011.1. [Q8VHL0-1 ]
NP_082398.1. NM_028122.4. [Q8VHL0-1 ]
UniGenei Mm.33832.

3D structure databases

ProteinModelPortali Q8VHL0.
SMRi Q8VHL0. Positions 31-376.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000025433.

Chemistry

ChEMBLi CHEMBL2163171.

Proteomic databases

MaxQBi Q8VHL0.
PaxDbi Q8VHL0.
PRIDEi Q8VHL0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000091813 ; ENSMUSP00000089421 ; ENSMUSG00000059336 . [Q8VHL0-1 ]
ENSMUST00000160292 ; ENSMUSP00000125114 ; ENSMUSG00000059336 . [Q8VHL0-2 ]
ENSMUST00000160639 ; ENSMUSP00000125367 ; ENSMUSG00000059336 . [Q8VHL0-1 ]
GeneIDi 108052.
KEGGi mmu:108052.
UCSCi uc008fsc.2. mouse. [Q8VHL0-1 ]
uc008fsd.2. mouse. [Q8VHL0-2 ]

Organism-specific databases

CTDi 6563.
MGIi MGI:1351654. Slc14a1.

Phylogenomic databases

eggNOGi COG4413.
GeneTreei ENSGT00390000018729.
HOGENOMi HOG000065705.
HOVERGENi HBG000540.
InParanoidi Q3U542.
KOi K08716.
OMAi PNIYKMP.
OrthoDBi EOG7GBFX8.
TreeFami TF332858.

Enzyme and pathway databases

Reactomei REACT_217615. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

NextBioi 359947.
PROi Q8VHL0.
SOURCEi Search...

Gene expression databases

Bgeei Q8VHL0.
Genevestigatori Q8VHL0.

Family and domain databases

Gene3Di 1.10.3430.10. 1 hit.
InterProi IPR029020. Ammonium/urea_transptr.
IPR004937. Urea_transporter.
[Graphical view ]
PANTHERi PTHR10464. PTHR10464. 1 hit.
Pfami PF03253. UT. 1 hit.
[Graphical view ]
PIRSFi PIRSF016502. Urea_transporter. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Urea-selective concentrating defect in transgenic mice lacking urea transporter UT-B."
    Yang B., Bankir L., Gillespie A., Epstein C.J., Verkman A.S.
    J. Biol. Chem. 277:10633-10637(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "UT-B1 urea transporter is expressed along the urinary and gastrointestinal tracts of the mouse."
    Lucien N., Bruneval P., Lasbennes F., Belair M.F., Mandet C., Cartron J.P., Bailly P., Trinh-Trang-Tan M.M.
    Am. J. Physiol. 288:R1046-R1056(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, INDUCTION.
    Strain: BALB/c.
    Tissue: Kidney.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Embryo and Thymus.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6 and C57BL/6NCr.
    Tissue: Eye, Head and Hematopoietic stem cell.
  6. "Analysis of double knockout mice lacking aquaporin-1 and urea transporter UT-B. Evidence for UT-B-facilitated water transport in erythrocytes."
    Yang B., Verkman A.S.
    J. Biol. Chem. 277:36782-36786(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiUT1_MOUSE
AccessioniPrimary (citable) accession number: Q8VHL0
Secondary accession number(s): Q3U542
, Q497G1, Q5RJG2, Q6PDP4, Q9CZX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: June 28, 2011
Last modified: September 3, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi