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Protein

Transmembrane protease serine 11D

Gene

Tmprss11d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play some biological role in the host defense system on the mucous membrane independently of or in cooperation with other substances in airway mucous or bronchial secretions (By similarity). Plays a role in the proteolytic processing of ACE2. Isoform 2 may play a key role in regulating adrenal proliferation by specifically cleaving N-POMC.By similarity1 Publication

Catalytic activityi

Preferentially cleaves the C-terminal side of arginine residues at the P1 position of certain peptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Charge relay systemBy similarity
Active sitei271 – 2711Charge relay systemBy similarity
Active sitei367 – 3671Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protease serine 11D (EC:3.4.21.-)
Alternative name(s):
Adrenal secretory serine protease
Short name:
AsP
Airway trypsin-like protease
Short name:
AT
Cleaved into the following 2 chains:
Gene namesi
Name:Tmprss11d
Synonyms:Mat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2385221. Tmprss11d.

Subcellular locationi

Chain Transmembrane protease serine 11D catalytic chain :
  • Secreted By similarity

  • Note: Activated by cleavage and secreted.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717CytoplasmicSequence analysisAdd
BLAST
Transmembranei18 – 3821Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini39 – 417379ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Transmembrane protease serine 11D non-catalytic chainPRO_0000027887Add
BLAST
Chaini186 – 417232Transmembrane protease serine 11D catalytic chainPRO_0000027888Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi172 ↔ 291Interchain (between non-catalytic and catalytic chains)PROSITE-ProRule annotation
Disulfide bondi211 ↔ 227PROSITE-ProRule annotation
Disulfide bondi336 ↔ 352PROSITE-ProRule annotation
Disulfide bondi363 ↔ 392PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ8VHK8.
PRIDEiQ8VHK8.

Expressioni

Tissue specificityi

Highly expressed in the esophagus, tongue, and trachea, low expression was seen in heart, lung, and adrenal gland. Isoform 2 is also highly expressed in the adrenal gland.1 Publication

Gene expression databases

BgeeiQ8VHK8.
CleanExiMM_TMPRSS11D.
GenevisibleiQ8VHK8. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031175.

Structurei

Secondary structure

1
417
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi46 – 6015Combined sources
Helixi63 – 664Combined sources
Helixi71 – 8919Combined sources
Turni92 – 965Combined sources
Beta strandi97 – 10812Combined sources
Beta strandi111 – 12212Combined sources
Helixi128 – 13912Combined sources
Helixi140 – 1423Combined sources
Beta strandi144 – 1507Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E7VX-ray1.92A44-164[»]
ProteinModelPortaliQ8VHK8.
SMRiQ8VHK8. Positions 45-163, 186-417.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VHK8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 162117SEAPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 417232Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SEA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000251823.
HOVERGENiHBG013304.
InParanoidiQ8VHK8.
KOiK09641.
OMAiSNLRNQF.
OrthoDBiEOG75B84T.
PhylomeDBiQ8VHK8.
TreeFamiTF351684.

Family and domain databases

Gene3Di3.30.70.960. 1 hit.
InterProiIPR017329. Pept_S1A_HAT/DESC1.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF01390. SEA. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF037941. TMPRSS11ABCDE. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF82671. SSF82671. 1 hit.
PROSITEiPS50024. SEA. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VHK8-1) [UniParc]FASTAAdd to basket

Also known as: MAT1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRPRPMLSP SRFFTPFAVA FVVIITVGLL AMMAGLLIHF LAFDKKAYFY
60 70 80 90 100
HSSFQILNVE YTEALNSPAT HEYRTLSERI EAMITDEFRG SSLKSEFIRT
110 120 130 140 150
HVVKLRKEGT GVVADVVMKF RSSKRNNRKV MKTRIQSVLR RLSSSGNLEI
160 170 180 190 200
APSNEITSLT DQDTENVLTQ ECGARPDLIT LSEERIIGGM QAEPGDWPWQ
210 220 230 240 250
VSLQLNNVHH CGGALISNMW VLTAAHCFKS YPNPQYWTAT FGVSTMSPRL
260 270 280 290 300
RVRVRAILAH DGYSSVTRDN DIAVVQLDRS VAFSRNIHRV CLPAATQNII
310 320 330 340 350
PGSVAYVTGW GSLTYGGNAV TNLRQGEVRI ISSEECNTPA GYSGSVLPGM
360 370 380 390 400
LCAGMRSGAV DACQGDSGGP LVQEDSRRLW FVVGIVSWGY QCGLPNKPGV
410
YTRVTAYRNW IRQQTGI
Length:417
Mass (Da):46,254
Last modified:July 5, 2005 - v2
Checksum:iC7FACD21C06FEAEA
GO
Isoform 2 (identifier: Q8VHK8-2) [UniParc]FASTAAdd to basket

Also known as: MAT2

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     139-158: LRRLSSSGNLEIAPSNEITS → MIFSFCFVDFVLTFSFLMIA

Show »
Length:279
Mass (Da):30,593
Checksum:i7A8CF885250910F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431F → L in AAL47139 (PubMed:14691009).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 138138Missing in isoform 2. 1 PublicationVSP_014519Add
BLAST
Alternative sequencei139 – 15820LRRLS…NEITS → MIFSFCFVDFVLTFSFLMIA in isoform 2. 1 PublicationVSP_014520Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448809 mRNA. Translation: AAL47139.1.
AF539752 mRNA. Translation: AAP97729.1.
AB053953 mRNA. Translation: BAD89353.1.
BC020151 mRNA. Translation: AAH20151.1.
CCDSiCCDS39124.1. [Q8VHK8-1]
RefSeqiNP_663536.1. NM_145561.2. [Q8VHK8-1]
UniGeneiMm.240139.

Genome annotation databases

EnsembliENSMUST00000031175; ENSMUSP00000031175; ENSMUSG00000061259. [Q8VHK8-1]
ENSMUST00000122377; ENSMUSP00000113079; ENSMUSG00000061259. [Q8VHK8-2]
GeneIDi231382.
KEGGimmu:231382.
UCSCiuc008xxo.1. mouse. [Q8VHK8-2]
uc008xxp.1. mouse. [Q8VHK8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448809 mRNA. Translation: AAL47139.1.
AF539752 mRNA. Translation: AAP97729.1.
AB053953 mRNA. Translation: BAD89353.1.
BC020151 mRNA. Translation: AAH20151.1.
CCDSiCCDS39124.1. [Q8VHK8-1]
RefSeqiNP_663536.1. NM_145561.2. [Q8VHK8-1]
UniGeneiMm.240139.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E7VX-ray1.92A44-164[»]
ProteinModelPortaliQ8VHK8.
SMRiQ8VHK8. Positions 45-163, 186-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031175.

Proteomic databases

PaxDbiQ8VHK8.
PRIDEiQ8VHK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031175; ENSMUSP00000031175; ENSMUSG00000061259. [Q8VHK8-1]
ENSMUST00000122377; ENSMUSP00000113079; ENSMUSG00000061259. [Q8VHK8-2]
GeneIDi231382.
KEGGimmu:231382.
UCSCiuc008xxo.1. mouse. [Q8VHK8-2]
uc008xxp.1. mouse. [Q8VHK8-1]

Organism-specific databases

CTDi9407.
MGIiMGI:2385221. Tmprss11d.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000251823.
HOVERGENiHBG013304.
InParanoidiQ8VHK8.
KOiK09641.
OMAiSNLRNQF.
OrthoDBiEOG75B84T.
PhylomeDBiQ8VHK8.
TreeFamiTF351684.

Miscellaneous databases

EvolutionaryTraceiQ8VHK8.
NextBioi380532.
PROiQ8VHK8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VHK8.
CleanExiMM_TMPRSS11D.
GenevisibleiQ8VHK8. MM.

Family and domain databases

Gene3Di3.30.70.960. 1 hit.
InterProiIPR017329. Pept_S1A_HAT/DESC1.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF01390. SEA. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF037941. TMPRSS11ABCDE. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF82671. SSF82671. 1 hit.
PROSITEiPS50024. SEA. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The adrenal secretory serine protease AsP is a short secretory isoform of the transmembrane airway trypsin-like protease."
    Hansen I.A., Fassnacht M., Hahner S., Hammer F., Schammann M., Meyer S.R., Bicknell A.B., Allolio B.
    Endocrinology 145:1898-1905(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    Strain: Czech II.
    Tissue: Tongue and Trachea.
  2. "Molecular cloning and expression of murine homologue of human airway trypsin-like protease gene."
    Eguchi H., Yamamura S., Yamaoka K., Masegi T., Kamimura T., Yasuoka S.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary tumor.
  4. "TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein."
    Heurich A., Hofmann-Winkler H., Gierer S., Liepold T., Jahn O., Poehlmann S.
    J. Virol. 88:1293-1307(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Crystal structure of SEA domain of transmembrane protease from Mus musculus."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUL-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 44-164.

Entry informationi

Entry nameiTM11D_MOUSE
AccessioniPrimary (citable) accession number: Q8VHK8
Secondary accession number(s): Q7TNX3, Q8VDV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: May 11, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.