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Protein

Hepatoma-derived growth factor

Gene

Hdgf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19HeparinBy similarity1
Binding sitei21HeparinBy similarity1
Binding sitei72HeparinBy similarity1
Binding sitei75HeparinBy similarity1
Binding sitei79HeparinBy similarity1
Binding sitei80HeparinBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • protein localization to nucleus Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Heparin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatoma-derived growth factor
Short name:
HDGF
Gene namesi
Name:Hdgf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70961. Hdgf.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917021 – 237Hepatoma-derived growth factorAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44N6-acetyllysineBy similarity1
Cross-linki80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei132PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei200PhosphothreonineCombined sources1
Modified residuei202PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei236PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated with SUMO1. Sumoylation prevents binding to chromatin (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8VHK7.
PRIDEiQ8VHK7.

PTM databases

iPTMnetiQ8VHK7.
PhosphoSitePlusiQ8VHK7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012836.

Interactioni

Subunit structurei

Monomer, and domain-swapped homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017234.

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 20Combined sources6
Beta strandi23 – 32Combined sources10
Beta strandi45 – 49Combined sources5
Turni50 – 53Combined sources4
Beta strandi54 – 58Combined sources5
Helixi60 – 62Combined sources3
Beta strandi63 – 65Combined sources3
Helixi66 – 73Combined sources8
Beta strandi78 – 81Combined sources4
Helixi82 – 91Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B8ANMR-A1-110[»]
ProteinModelPortaliQ8VHK7.
SMRiQ8VHK7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VHK7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 69PWWPPROSITE-ProRule annotationAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi75 – 80Nuclear localization signalBy similarity6
Motifi155 – 170Bipartite nuclear localization signalBy similarityAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi111 – 227Glu-richAdd BLAST117

Domaini

The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.By similarity

Sequence similaritiesi

Belongs to the HDGF family.Curated
Contains 1 PWWP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410Y5WD. LUCA.
HOGENOMiHOG000112874.
HOVERGENiHBG010574.
InParanoidiQ8VHK7.
KOiK16641.
PhylomeDBiQ8VHK7.

Family and domain databases

InterProiIPR000313. PWWP_dom.
[Graphical view]
PfamiPF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VHK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF
60 70 80 90 100
GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY
110 120 130 140 150
QSSQKKSCAE EPEVEPEAHE GDGDKKGNAE GSSDEEGKLV IDEPAKEKNE
160 170 180 190 200
KGMLKRRAGD MLEDSPKRPK ESGDHEEEEK EIAALEGERP LPVEMEKNST
210 220 230
PSEPDSGQGP PPEEEEGEEE AAKEEAEAQG VRDHESL
Length:237
Mass (Da):26,488
Last modified:September 27, 2004 - v2
Checksum:iC120F798BD40D360
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231V → I in AAL47132 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448810 mRNA. Translation: AAL47132.1.
AF389348 mRNA. Translation: AAK72966.1.
BC070943 mRNA. Translation: AAH70943.1.
RefSeqiNP_446159.1. NM_053707.2.
UniGeneiRn.860.

Genome annotation databases

GeneIDi114499.
KEGGirno:114499.
UCSCiRGD:70961. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448810 mRNA. Translation: AAL47132.1.
AF389348 mRNA. Translation: AAK72966.1.
BC070943 mRNA. Translation: AAH70943.1.
RefSeqiNP_446159.1. NM_053707.2.
UniGeneiRn.860.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B8ANMR-A1-110[»]
ProteinModelPortaliQ8VHK7.
SMRiQ8VHK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017234.

PTM databases

iPTMnetiQ8VHK7.
PhosphoSitePlusiQ8VHK7.

Proteomic databases

PaxDbiQ8VHK7.
PRIDEiQ8VHK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114499.
KEGGirno:114499.
UCSCiRGD:70961. rat.

Organism-specific databases

CTDi3068.
RGDi70961. Hdgf.

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410Y5WD. LUCA.
HOGENOMiHOG000112874.
HOVERGENiHBG010574.
InParanoidiQ8VHK7.
KOiK16641.
PhylomeDBiQ8VHK7.

Miscellaneous databases

EvolutionaryTraceiQ8VHK7.
PROiQ8VHK7.

Gene expression databases

BgeeiENSRNOG00000012836.

Family and domain databases

InterProiIPR000313. PWWP_dom.
[Graphical view]
PfamiPF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHDGF_RAT
AccessioniPrimary (citable) accession number: Q8VHK7
Secondary accession number(s): Q923W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: September 27, 2004
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.