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Protein

Hepatoma-derived growth factor

Gene

Hdgf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191HeparinBy similarity
Binding sitei21 – 211HeparinBy similarity
Binding sitei72 – 721HeparinBy similarity
Binding sitei75 – 751HeparinBy similarity
Binding sitei79 – 791HeparinBy similarity
Binding sitei80 – 801HeparinBy similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • protein localization to nucleus Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Heparin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatoma-derived growth factor
Short name:
HDGF
Gene namesi
Name:Hdgf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70961. Hdgf.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Hepatoma-derived growth factorPRO_0000191702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441N6-acetyllysineBy similarity
Cross-linki80 – 80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki80 – 80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei132 – 1321PhosphoserineCombined sources
Modified residuei133 – 1331PhosphoserineCombined sources
Modified residuei165 – 1651PhosphoserineCombined sources
Modified residuei199 – 1991PhosphoserineCombined sources
Modified residuei200 – 2001PhosphothreonineCombined sources
Modified residuei202 – 2021PhosphoserineCombined sources
Modified residuei206 – 2061PhosphoserineCombined sources
Modified residuei236 – 2361PhosphoserineBy similarity

Post-translational modificationi

Sumoylated with SUMO1. Sumoylation prevents binding to chromatin (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8VHK7.
PRIDEiQ8VHK7.

PTM databases

iPTMnetiQ8VHK7.
PhosphoSiteiQ8VHK7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012836.

Interactioni

Subunit structurei

Monomer, and domain-swapped homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017234.

Structurei

Secondary structure

1
237
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 206Combined sources
Beta strandi23 – 3210Combined sources
Beta strandi45 – 495Combined sources
Turni50 – 534Combined sources
Beta strandi54 – 585Combined sources
Helixi60 – 623Combined sources
Beta strandi63 – 653Combined sources
Helixi66 – 738Combined sources
Beta strandi78 – 814Combined sources
Helixi82 – 9110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B8ANMR-A1-110[»]
ProteinModelPortaliQ8VHK7.
SMRiQ8VHK7. Positions 1-110.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VHK7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 6958PWWPPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi75 – 806Nuclear localization signalBy similarity
Motifi155 – 17016Bipartite nuclear localization signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi111 – 227117Glu-richAdd
BLAST

Domaini

The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.By similarity

Sequence similaritiesi

Belongs to the HDGF family.Curated
Contains 1 PWWP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410Y5WD. LUCA.
HOGENOMiHOG000112874.
HOVERGENiHBG010574.
InParanoidiQ8VHK7.
KOiK16641.
PhylomeDBiQ8VHK7.

Family and domain databases

InterProiIPR000313. PWWP_dom.
[Graphical view]
PfamiPF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VHK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF
60 70 80 90 100
GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY
110 120 130 140 150
QSSQKKSCAE EPEVEPEAHE GDGDKKGNAE GSSDEEGKLV IDEPAKEKNE
160 170 180 190 200
KGMLKRRAGD MLEDSPKRPK ESGDHEEEEK EIAALEGERP LPVEMEKNST
210 220 230
PSEPDSGQGP PPEEEEGEEE AAKEEAEAQG VRDHESL
Length:237
Mass (Da):26,488
Last modified:September 27, 2004 - v2
Checksum:iC120F798BD40D360
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311V → I in AAL47132 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448810 mRNA. Translation: AAL47132.1.
AF389348 mRNA. Translation: AAK72966.1.
BC070943 mRNA. Translation: AAH70943.1.
RefSeqiNP_446159.1. NM_053707.2.
UniGeneiRn.860.

Genome annotation databases

GeneIDi114499.
KEGGirno:114499.
UCSCiRGD:70961. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448810 mRNA. Translation: AAL47132.1.
AF389348 mRNA. Translation: AAK72966.1.
BC070943 mRNA. Translation: AAH70943.1.
RefSeqiNP_446159.1. NM_053707.2.
UniGeneiRn.860.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B8ANMR-A1-110[»]
ProteinModelPortaliQ8VHK7.
SMRiQ8VHK7. Positions 1-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017234.

PTM databases

iPTMnetiQ8VHK7.
PhosphoSiteiQ8VHK7.

Proteomic databases

PaxDbiQ8VHK7.
PRIDEiQ8VHK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114499.
KEGGirno:114499.
UCSCiRGD:70961. rat.

Organism-specific databases

CTDi3068.
RGDi70961. Hdgf.

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410Y5WD. LUCA.
HOGENOMiHOG000112874.
HOVERGENiHBG010574.
InParanoidiQ8VHK7.
KOiK16641.
PhylomeDBiQ8VHK7.

Miscellaneous databases

EvolutionaryTraceiQ8VHK7.
PROiQ8VHK7.

Gene expression databases

BgeeiENSRNOG00000012836.

Family and domain databases

InterProiIPR000313. PWWP_dom.
[Graphical view]
PfamiPF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHDGF_RAT
AccessioniPrimary (citable) accession number: Q8VHK7
Secondary accession number(s): Q923W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: September 27, 2004
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.