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Q8VHJ4 (TM11D_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transmembrane protease serine 11D
EC=3.4.21.-
Alternative name(s):
Adrenal secretory serine protease
Short name=AsP
Airway trypsin-like protease
Short name=AT

Cleaved into the following 2 chains:

  1. Transmembrane protease serine 11D non-catalytic chain
  2. Transmembrane protease serine 11D catalytic chain
Gene names
Name:Tmprss11d
Synonyms:Rat
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play some biological role in the host defense system on the mucous membrane, independently of, or in cooperation with other substances in airway mucous or bronchial secretions By similarity. Isoform 2 may play a key role in regulating adrenal proliferation by specifically cleaving N-POMC. Ref.1

Catalytic activity

Preferentially cleaves the C-terminal side of arginine residues at the P1 position of certain peptides.

Subunit structure

Monomer By similarity.

Subcellular location

Cell membrane; Single-pass type II membrane protein By similarity.

Transmembrane protease serine 11D catalytic chain: Secreted By similarity. Note: Activated by cleavage and secreted By similarity.

Tissue specificity

Isoform 1 and isoform 2 are expressed in the esophagus, tongue and trachea. Isoform 2 is also highly expressed in the adrenal cortex and heart. Ref.1 Ref.2

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Contains 1 SEA domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VHJ4-1)

Also known as: RAT1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VHJ4-2)

Also known as: RAT2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     139-158: LQRLSSSGNLEIAPSNGITS → MSFSFCFVDLLLVLSFLTLA
Note: Secreted and retained on the cell surface after secretion.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Transmembrane protease serine 11D non-catalytic chain
PRO_0000027889
Chain186 – 417232Transmembrane protease serine 11D catalytic chain
PRO_0000027890

Regions

Topological domain1 – 1717Cytoplasmic Potential
Transmembrane18 – 3821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain39 – 417379Extracellular Potential
Domain41 – 165125SEA
Domain186 – 416231Peptidase S1

Sites

Active site2261Charge relay system By similarity
Active site2711Charge relay system By similarity
Active site3671Charge relay system By similarity

Amino acid modifications

Disulfide bond172 ↔ 291Interchain (between non-catalytic and catalytic chains) By similarity
Disulfide bond211 ↔ 227 By similarity
Disulfide bond336 ↔ 352 By similarity
Disulfide bond363 ↔ 392 By similarity

Natural variations

Alternative sequence1 – 138138Missing in isoform 2.
VSP_014521
Alternative sequence139 – 15820LQRLS…NGITS → MSFSFCFVDLLLVLSFLTLA in isoform 2.
VSP_014522

Experimental info

Sequence conflict3001M → I in AAF13253. Ref.1
Sequence conflict3071V → A in AAF13253. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (RAT1) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: DB9504158B018E21

FASTA41746,288
        10         20         30         40         50         60 
MYRPRSMVSP SRFFNPFMVA LIVIITVGLL AMTAGLLIHF LAFDKRAYFY HSNFHILNVD 

        70         80         90        100        110        120 
YTEALNSPAT HEYRTLSERI ESMITDAFRE SNLRSEFIRT HVVKLRKEGS GVVADVVMKF 

       130        140        150        160        170        180 
RSSKRNNKKA IKTRIQSVLQ RLSSSGNLEI APSNGITSLT DQDTENVLTQ ECGARPDLIT 

       190        200        210        220        230        240 
LSEERIIGGT QAETGDWPWQ VSLQLNNVHH CGGTLISNLW VLTAAHCFRS YSNPQQWTAT 

       250        260        270        280        290        300 
FGVSTISPRL RVRVRAILAH AEYNSITRDN DIAVVQLDRP VTFTRNIHRV CLPAATQNIM 

       310        320        330        340        350        360 
PDSVAYVTGW GSLTYGGNTV TNLQQGEVRI VSSEVCNEPA GYGGSVLPGM LCAGVRSGAV 

       370        380        390        400        410 
DACQGDSGGP LVQEDTRRLW FVVGIVSWGY QCGLPNKPGV YTRVTAYRNW IRQQTGI

« Hide

Isoform 2 (RAT2) [UniParc].

Checksum: 8745B04284971A81
Show »

FASTA27930,569

References

[1]"Characterization of a serine protease that cleaves pro-gamma-melanotropin at the adrenal to stimulate growth."
Bicknell A.B., Lomthaisong K., Woods R.J., Hutchinson E.G., Bennett H.P.J., Gladwell R.T., Lowry P.J.
Cell 105:903-912(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
Strain: New England Deaconess Hospital.
[2]"The adrenal secretory serine protease AsP is a short secretory isoform of the transmembrane airway trypsin-like protease."
Hansen I.A., Fassnacht M., Hahner S., Hammer F., Schammann M., Meyer S.R., Bicknell A.B., Allolio B.
Endocrinology 145:1898-1905(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
Strain: Wistar.
Tissue: Trachea.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF198087 mRNA. Translation: AAF13253.1.
AF453776 mRNA. Translation: AAL50817.1.
IPIIPI00389746.
IPI00608155.
RefSeqNP_001028824.1. NM_001033652.1.
NP_072152.1. NM_022630.1.
UniGeneRn.48747.

3D structure databases

ProteinModelPortalQ8VHJ4.
SMRQ8VHJ4. Positions 45-163.
ModBaseSearch...

Protein family/group databases

MEROPSS01.047.

Proteomic databases

PRIDEQ8VHJ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID64565.
KEGGrno:64565.
UCSCRGD:620654. rat.

Organism-specific databases

CTD9407.
RGD620654. Tmprss11d.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251823.
HOVERGENHBG013304.
KOK09641.

Gene expression databases

ArrayExpressQ8VHJ4.
GenevestigatorQ8VHJ4.
GermOnlineENSRNOG00000002006. Rattus norvegicus.

Family and domain databases

InterProIPR017329. Pept_S1A_HAT/DESC1.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000082. SEA.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF01390. SEA. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFPIRSF037941. TMPRSS11ABCDE. 1 hit.
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00200. SEA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS50024. SEA. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613502.

Entry information

Entry nameTM11D_RAT
AccessionPrimary (citable) accession number: Q8VHJ4
Secondary accession number(s): Q9QZ74
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: March 1, 2002
Last modified: April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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