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Protein

Angiomotin

Gene

Amot

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • blood vessel endothelial cell migration Source: MGI
  • blood vessel morphogenesis Source: MGI
  • cell migration involved in gastrulation Source: MGI
  • cellular protein localization Source: MGI
  • chemotaxis Source: MGI
  • establishment of cell polarity involved in ameboidal cell migration Source: MGI
  • gastrulation with mouth forming second Source: MGI
  • hippo signaling Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of angiogenesis Source: MGI
  • negative regulation of GTPase activity Source: MGI
  • negative regulation of vascular permeability Source: MGI
  • positive regulation of blood vessel endothelial cell migration Source: MGI
  • positive regulation of embryonic development Source: MGI
  • regulation of cell migration Source: MGI
  • regulation of small GTPase mediated signal transduction Source: MGI
  • vasculogenesis Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiomotin
Gene namesi
Name:Amot
Synonyms:Kiaa1071
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:108440. Amot.

Subcellular locationi

  • Cell junctiontight junction By similarity

  • Note: Localized on the cell surface. May act as a transmembrane protein (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11261126AngiomotinPRO_0000190669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki573 – 573Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki574 – 574Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei693 – 6931PhosphoserineCombined sources

Post-translational modificationi

Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8VHG2.
PaxDbiQ8VHG2.
PeptideAtlasiQ8VHG2.
PRIDEiQ8VHG2.

PTM databases

iPTMnetiQ8VHG2.
PhosphoSiteiQ8VHG2.

Expressioni

Tissue specificityi

Expressed in brain, skeletal muscle and placenta.1 Publication

Gene expression databases

BgeeiQ8VHG2.
CleanExiMM_AMOT.
ExpressionAtlasiQ8VHG2. baseline and differential.
GenevisibleiQ8VHG2. MM.

Interactioni

Subunit structurei

Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with MAGI1 and angiostatin (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205274. 17 interactions.
IntActiQ8VHG2. 9 interactions.
MINTiMINT-4120151.
STRINGi10090.ENSMUSP00000108455.

Structurei

3D structure databases

ProteinModelPortaliQ8VHG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili408 – 668261Sequence analysisAdd
BLAST
Coiled coili700 – 73031Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1123 – 11264PDZ-bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi855 – 988134Ala-richAdd
BLAST

Domaini

The angiostatin binding domain (850-1047) allows the binding to angiostatin.
The coiled coil domain interacts directly with the BAR domain of ARHGAP17.By similarity

Sequence similaritiesi

Belongs to the angiomotin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJU5. Eukaryota.
ENOG410XSY2. LUCA.
GeneTreeiENSGT00530000063846.
HOVERGENiHBG066485.
InParanoidiQ8VHG2.
KOiK16819.
OMAiELDGCYE.
PhylomeDBiQ8VHG2.
TreeFamiTF333368.

Family and domain databases

InterProiIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PfamiPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSiPR01807. ANGIOMOTIN.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VHG2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSSDDQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNSSPFST
60 70 80 90 100
GSGNQGPQND VLSSQDHHQQ QLVAHPARQE PQGQEIQSEN GVMEKQLSPR
110 120 130 140 150
MQNNEELPTY EEAKVQSQYF RGQQHASVGA AFYVTGVTNQ KMRTEGRPSV
160 170 180 190 200
QRLTPGKMHQ DEGLRDLKQG HVRSLSERLM QMSLATSGVK AHPPVTSAPL
210 220 230 240 250
SPPQPNDLYK NATSSSEFYK AQGPPPSQHS LKGMEHRGPP PEYPFKGVPS
260 270 280 290 300
QSVVCKSQEP GHFYSEHRLN QPGRTEGQLM RYQHPPEYGA ARATQDISSL
310 320 330 340 350
SLSARNSQPH SPTSSLTAGA SSLPLLQSPP STRLPPGQHL VSNQGDHSAH
360 370 380 390 400
LSRHQQHLLS SQSHQGDHYR HAQASLTSAQ QQPGEAYSAM PRAQQSASYQ
410 420 430 440 450
PMPADPFAMV SRAQQMVEIL SDENRNLRQE LDGCYEKVAR LQKVETEIQR
460 470 480 490 500
VSEAYENLVK SSSKREALEK AMRNKLEGEI RRMHDFNRDL RDRLETANKQ
510 520 530 540 550
LAEKEYEGSE DTRKTISQLF AKHKENQREK EKLEAELATA RSTNEDQRRH
560 570 580 590 600
IEIRDQALSN AQAKVVKLEE ELKKKQVYVD KVEKMQQALV QLQAACEKRE
610 620 630 640 650
QLEHRLRTRL ERELESLRIQ QRQGNSQPTN ASEYNAAALM ELLREKEERI
660 670 680 690 700
LALEADMTKW EQKYLEENVM RHFALDAAAT VAAQRDTTVI SHSPNTSYDT
710 720 730 740 750
ALEARIQKEE EEILMANKRC LDMEGRIKTL HAQIIEKDAM IKVLQQRSRK
760 770 780 790 800
EPSKTEQLSS MRPAKSLMSI SNAGSGLLAH SSTLTGAPIM EEKRDDKSWK
810 820 830 840 850
GSLGVLLGGD YRVEPVPSTP SPVPPSTPLL SAHSKTGSRD CSTQTERGPE
860 870 880 890 900
STKTAAVTPI SAPMAGPVAA AAPAAAINAT AATNTATAAT NTTIMVAAAP
910 920 930 940 950
VAVAAVAAPA AAAATPSPAN AAALAAAAAP ATSVSAATSV SAANSISPAA
960 970 980 990 1000
PVAPAAVVPP AAPVSPAAAV QIPAAASLTP ATVSPTAATA TAAVAAATTA
1010 1020 1030 1040 1050
AITAAAAAAT TAIQVAPATS APVPSPASIP APATAQASAP TPTQASTPAP
1060 1070 1080 1090 1100
TEPPSPVPTP TPALVQTEGP ANPGASSGPR RLSTPNLMCN PDKPDAPAFH
1110 1120
SSTLERKTPI QILGQEPDAE MVEYLI
Length:1,126
Mass (Da):120,915
Last modified:July 24, 2007 - v3
Checksum:i530D84854E943CD4
GO
Isoform 2 (identifier: Q8VHG2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     805-853: VLLGGDYRVE...QTERGPESTK → KEKESNRSKG...ESPLSMELDL
     854-1126: Missing.

Show »
Length:853
Mass (Da):95,963
Checksum:i75463F4AC487A03D
GO

Sequence cautioni

The sequence AAH57638.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAL73436.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAM22158.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAM22159.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti298 – 2981S → Y in AAL73436 (PubMed:12406577).Curated
Sequence conflicti342 – 3421S → P in AAL73436 (PubMed:12406577).Curated
Sequence conflicti342 – 3421S → P in AAH57638 (PubMed:15489334).Curated
Sequence conflicti409 – 4091M → I in AAL73436 (PubMed:12406577).Curated
Sequence conflicti409 – 4091M → I in AAH57638 (PubMed:15489334).Curated
Sequence conflicti525 – 55632ENQRE…EIRDQ → FCQPYNPAERKAEVRGGRFT IEAQRGHIKIRAR in AAL73436 (PubMed:12406577).CuratedAdd
BLAST
Sequence conflicti565 – 5662VV → W in AAL73436 (PubMed:12406577).Curated
Sequence conflicti735 – 7351I → M in AAL73436 (PubMed:12406577).Curated
Sequence conflicti752 – 7543PSK → RE in AAL73436 (PubMed:12406577).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei805 – 85349VLLGG…PESTK → KEKESNRSKGTVTDLESVLT LLHTARKRDNGPGSREENLE SPLSMELDL in isoform 2. 1 PublicationVSP_027108Add
BLAST
Alternative sequencei854 – 1126273Missing in isoform 2. 1 PublicationVSP_027109Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL807753 Genomic DNA. Translation: CAM22158.1. Different initiation.
AL807753 Genomic DNA. Translation: CAM22159.1. Different initiation.
BC057638 mRNA. Translation: AAH57638.1. Different initiation.
AF461135 mRNA. Translation: AAL73436.1. Different initiation.
AK129277 mRNA. Translation: BAC98087.1.
CCDSiCCDS72443.1. [Q8VHG2-1]
RefSeqiNP_001277203.1. NM_001290274.1. [Q8VHG2-2]
NP_695231.3. NM_153319.3. [Q8VHG2-1]
XP_006528927.1. XM_006528864.2. [Q8VHG2-1]
XP_011246130.1. XM_011247828.1. [Q8VHG2-1]
UniGeneiMm.100068.

Genome annotation databases

EnsembliENSMUST00000112836; ENSMUSP00000108455; ENSMUSG00000041688. [Q8VHG2-1]
GeneIDi27494.
KEGGimmu:27494.
UCSCiuc009una.2. mouse. [Q8VHG2-1]
uc009unb.2. mouse. [Q8VHG2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL807753 Genomic DNA. Translation: CAM22158.1. Different initiation.
AL807753 Genomic DNA. Translation: CAM22159.1. Different initiation.
BC057638 mRNA. Translation: AAH57638.1. Different initiation.
AF461135 mRNA. Translation: AAL73436.1. Different initiation.
AK129277 mRNA. Translation: BAC98087.1.
CCDSiCCDS72443.1. [Q8VHG2-1]
RefSeqiNP_001277203.1. NM_001290274.1. [Q8VHG2-2]
NP_695231.3. NM_153319.3. [Q8VHG2-1]
XP_006528927.1. XM_006528864.2. [Q8VHG2-1]
XP_011246130.1. XM_011247828.1. [Q8VHG2-1]
UniGeneiMm.100068.

3D structure databases

ProteinModelPortaliQ8VHG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205274. 17 interactions.
IntActiQ8VHG2. 9 interactions.
MINTiMINT-4120151.
STRINGi10090.ENSMUSP00000108455.

PTM databases

iPTMnetiQ8VHG2.
PhosphoSiteiQ8VHG2.

Proteomic databases

MaxQBiQ8VHG2.
PaxDbiQ8VHG2.
PeptideAtlasiQ8VHG2.
PRIDEiQ8VHG2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112836; ENSMUSP00000108455; ENSMUSG00000041688. [Q8VHG2-1]
GeneIDi27494.
KEGGimmu:27494.
UCSCiuc009una.2. mouse. [Q8VHG2-1]
uc009unb.2. mouse. [Q8VHG2-2]

Organism-specific databases

CTDi154796.
MGIiMGI:108440. Amot.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IJU5. Eukaryota.
ENOG410XSY2. LUCA.
GeneTreeiENSGT00530000063846.
HOVERGENiHBG066485.
InParanoidiQ8VHG2.
KOiK16819.
OMAiELDGCYE.
PhylomeDBiQ8VHG2.
TreeFamiTF333368.

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.

Miscellaneous databases

ChiTaRSiAmot. mouse.
PROiQ8VHG2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VHG2.
CleanExiMM_AMOT.
ExpressionAtlasiQ8VHG2. baseline and differential.
GenevisibleiQ8VHG2. MM.

Family and domain databases

InterProiIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PfamiPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSiPR01807. ANGIOMOTIN.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 183-1126 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Angiomotin belongs to a novel protein family with conserved coiled-coil and PDZ binding domains."
    Bratt A., Wilson W.J., Troyanovsky B., Aase K., Kessler R., Van Meir E.G., Holmgren L.
    Gene 298:69-77(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 199-1126 (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: B6CBAF2.
    Tissue: Placenta.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 588-1126 (ISOFORM 1).
    Tissue: Embryonic tail.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-693, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung and Pancreas.

Entry informationi

Entry nameiAMOT_MOUSE
AccessioniPrimary (citable) accession number: Q8VHG2
Secondary accession number(s): A2AMJ9
, A2AMK0, Q6PFB8, Q6ZPZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 24, 2007
Last modified: July 6, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

'Motus' means 'motility' in Latin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.