Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8VHF5

- CISY_RAT

UniProt

Q8VHF5 - CISY_RAT

Protein

Citrate synthase, mitochondrial

Gene

Cs

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei301 – 3011PROSITE-ProRule annotation
    Active sitei347 – 3471PROSITE-ProRule annotation
    Active sitei402 – 4021PROSITE-ProRule annotation

    GO - Molecular functioni

    1. citrate (Si)-synthase activity Source: UniProtKB

    GO - Biological processi

    1. acetyl-CoA metabolic process Source: RGD
    2. carbohydrate metabolic process Source: UniProtKB
    3. cellular carbohydrate metabolic process Source: InterPro
    4. citrate metabolic process Source: RGD
    5. oxaloacetate metabolic process Source: RGD
    6. tricarboxylic acid cycle Source: RGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase, mitochondrial (EC:2.3.3.1)
    Alternative name(s):
    Citrate (Si)-synthase
    Gene namesi
    Name:Cs
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620330. Cs.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionBy similarityAdd
    BLAST
    Chaini28 – 466439Citrate synthase, mitochondrialPRO_0000253899Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei57 – 571N6-succinyllysineBy similarity
    Modified residuei76 – 761N6-acetyllysine; alternateBy similarity
    Modified residuei76 – 761N6-succinyllysine; alternateBy similarity
    Modified residuei103 – 1031N6-succinyllysineBy similarity
    Modified residuei193 – 1931N6-succinyllysineBy similarity
    Modified residuei321 – 3211N6-acetyllysine; alternateBy similarity
    Modified residuei321 – 3211N6-succinyllysine; alternateBy similarity
    Modified residuei327 – 3271N6-acetyllysine; alternateBy similarity
    Modified residuei327 – 3271N6-succinyllysine; alternateBy similarity
    Modified residuei375 – 3751N6-acetyllysine; alternateBy similarity
    Modified residuei375 – 3751N6-succinyllysine; alternateBy similarity
    Modified residuei382 – 3821N6-acetyllysineBy similarity
    Modified residuei393 – 3931N6-acetyllysine; alternateBy similarity
    Modified residuei393 – 3931N6-succinyllysine; alternateBy similarity
    Modified residuei395 – 3951N6,N6,N6-trimethyllysineBy similarity
    Modified residuei450 – 4501N6-succinyllysineBy similarity
    Modified residuei459 – 4591N6-acetyllysine; alternateBy similarity
    Modified residuei459 – 4591N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    PaxDbiQ8VHF5.
    PRIDEiQ8VHF5.

    2D gel databases

    World-2DPAGE0004:Q8VHF5.

    PTM databases

    PhosphoSiteiQ8VHF5.

    Expressioni

    Tissue specificityi

    Expressed in the head region and flagellum of epididymal sperm.1 Publication

    Gene expression databases

    GenevestigatoriQ8VHF5.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ8VHF5. 3 interactions.
    MINTiMINT-4609680.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VHF5.
    SMRiQ8VHF5. Positions 28-464.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0372.
    HOGENOMiHOG000130831.
    HOVERGENiHBG005336.
    InParanoidiQ8VHF5.
    KOiK01647.
    PhylomeDBiQ8VHF5.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VHF5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLTAAARL LGAKNSSCLV LAARHASASS TNLKDILSNL IPKEQARVKT    50
    FRQQHGKTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE 100
    CQKLLPKAKG GEEPLPEGLF WLLVTGQMPT EEQVSWLSQE WAKRAALPSH 150
    VVTMLDNFPT NLHPMSQLSA AITALNSESN FARAYAEGIN RTKYWELIYE 200
    DCMDLIAKLP CVAAKIYRNL YREGSSIGAI DSKLDWSHNF TNMLCYTEPQ 250
    FTELMRLYLT IHSDHDGGNV SAHTSHLVGS ALSDTYLSFA AAMNGLAGPL 300
    HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL 350
    RKTDPRYSCQ REFALKHLPK DPMFKLVAQL YKIVPNILLE QGKAKNPWPN 400
    VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK 450
    SMSTDGLMKF VDSKSG 466
    Length:466
    Mass (Da):51,867
    Last modified:March 1, 2002 - v1
    Checksum:iAF8D38379CD38124
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF461496 mRNA. Translation: AAL66372.1.
    RefSeqiNP_570111.1. NM_130755.1.
    UniGeneiRn.66581.

    Genome annotation databases

    GeneIDi170587.
    KEGGirno:170587.
    UCSCiRGD:620330. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF461496 mRNA. Translation: AAL66372.1 .
    RefSeqi NP_570111.1. NM_130755.1.
    UniGenei Rn.66581.

    3D structure databases

    ProteinModelPortali Q8VHF5.
    SMRi Q8VHF5. Positions 28-464.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VHF5. 3 interactions.
    MINTi MINT-4609680.

    PTM databases

    PhosphoSitei Q8VHF5.

    2D gel databases

    World-2DPAGE 0004:Q8VHF5.

    Proteomic databases

    PaxDbi Q8VHF5.
    PRIDEi Q8VHF5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 170587.
    KEGGi rno:170587.
    UCSCi RGD:620330. rat.

    Organism-specific databases

    CTDi 1431.
    RGDi 620330. Cs.

    Phylogenomic databases

    eggNOGi COG0372.
    HOGENOMi HOG000130831.
    HOVERGENi HBG005336.
    InParanoidi Q8VHF5.
    KOi K01647.
    PhylomeDBi Q8VHF5.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00717 .

    Miscellaneous databases

    NextBioi 621088.
    PROi Q8VHF5.

    Gene expression databases

    Genevestigatori Q8VHF5.

    Family and domain databases

    Gene3Di 1.10.580.10. 1 hit.
    InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    TIGRFAMsi TIGR01793. cit_synth_euk. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rattus norvegicus citrate synthase cDNA."
      Rossignol F., Jouaville L., Mounier R., Clottes E.
      Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 77-92; 184-191; 209-215; 223-233; 330-340; 383-393 AND 429-440, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
      Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
      Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCISY_RAT
    AccessioniPrimary (citable) accession number: Q8VHF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3