Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

MAP/microtubule affinity-regulating kinase 3

Gene

Mark3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by phosphorylation on Thr-211. Inhibited by phosphorylation on Thr-617 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851ATPPROSITE-ProRule annotation
Active sitei178 – 1781Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 709ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MAP/microtubule affinity-regulating kinase 3 (EC:2.7.11.1)
Gene namesi
Name:Mark3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619883. Mark3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 797797MAP/microtubule affinity-regulating kinase 3PRO_0000086306Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421PhosphoserineBy similarity
Modified residuei211 – 2111Phosphothreonine; by LKB1By similarity
Modified residuei368 – 3681PhosphoserineBy similarity
Modified residuei374 – 3741PhosphoserineCombined sources
Modified residuei380 – 3801PhosphoserineBy similarity
Modified residuei383 – 3831PhosphoserineBy similarity
Modified residuei400 – 4001PhosphoserineBy similarity
Modified residuei419 – 4191PhosphoserineBy similarity
Modified residuei469 – 4691PhosphoserineCombined sources
Modified residuei593 – 5931PhosphoserineBy similarity
Modified residuei596 – 5961PhosphoserineBy similarity
Modified residuei602 – 6021PhosphothreonineBy similarity
Modified residuei617 – 6171Phosphothreonine; by PKC/PRKCZBy similarity
Modified residuei651 – 6511PhosphoserineBy similarity
Modified residuei687 – 6871PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-617 by PRKCZ/aPKC inhibits the kinase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VHF0.
PRIDEiQ8VHF0.

PTM databases

iPTMnetiQ8VHF0.
PhosphoSiteiQ8VHF0.

Interactioni

Protein-protein interaction databases

BioGridi250933. 1 interaction.
IntActiQ8VHF0. 1 interaction.
STRINGi10116.ENSRNOP00000014395.

Structurei

3D structure databases

ProteinModelPortaliQ8VHF0.
SMRiQ8VHF0. Positions 47-367, 701-797.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 307252Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini326 – 36540UBAPROSITE-ProRule annotationAdd
BLAST
Domaini748 – 79750KA1PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi374 – 41239Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 KA1 (kinase-associated) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0586. Eukaryota.
ENOG410XNQ0. LUCA.
HOGENOMiHOG000233025.
HOVERGENiHBG052453.
InParanoidiQ8VHF0.
KOiK08798.
PhylomeDBiQ8VHF0.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VHF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHIS HGDGRQEVTS RTGRSGARCR NSIASCADEQ
60 70 80 90 100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL
110 120 130 140 150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM
160 170 180 190 200
KEKEARAKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKITDFGFS
210 220 230 240 250
NEFTVGSKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS
260 270 280 290 300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPVKRGTLEQ
310 320 330 340 350
IMKDRWINAG HEEEELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS
360 370 380 390 400
KMKYDEITAT YLLLGRKSAE LDASDSSSSS NLSLAKVRPS SDLSNSTGQS
410 420 430 440 450
PHHKGQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGV
460 470 480 490 500
PSRKSGSSAV GGKGIAPASP MLGNASNPNK ADIPERKKSP AVPSSNAASG
510 520 530 540 550
GMTRRNTYVC SERCAADRHS VIQNGKESSL TEVFAYAASP ASLCATSTCR
560 570 580 590 600
LRHQRSMSVS ASGHPKMVLP PIDSEGDTFK AITTPDQRTP VASTHSISSA
610 620 630 640 650
TTPDRIRFPR GTASRSTFHG QPRERRTATY NGPPASPSLS HEATPLSQTR
660 670 680 690 700
SRGSTNLFSK LTSKLTRRLP TEYERNGRYE GSSRNVSSEQ KDENREAKPR
710 720 730 740 750
SLRFTWSMKT TSSMDPSDMM REIRKVLDAN TCDYEQRERF LLFCVHGDGH
760 770 780 790
AESLVQWEME VCKLPRLSLN GVRFKRISGT SIAFKNIASK IANELKL
Length:797
Mass (Da):88,751
Last modified:March 1, 2002 - v1
Checksum:iE9834D5DB613ED37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465412 mRNA. Translation: AAL69981.1.
RefSeqiNP_570105.1. NM_130749.1.
UniGeneiRn.23232.

Genome annotation databases

GeneIDi170577.
KEGGirno:170577.
UCSCiRGD:619883. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465412 mRNA. Translation: AAL69981.1.
RefSeqiNP_570105.1. NM_130749.1.
UniGeneiRn.23232.

3D structure databases

ProteinModelPortaliQ8VHF0.
SMRiQ8VHF0. Positions 47-367, 701-797.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250933. 1 interaction.
IntActiQ8VHF0. 1 interaction.
STRINGi10116.ENSRNOP00000014395.

PTM databases

iPTMnetiQ8VHF0.
PhosphoSiteiQ8VHF0.

Proteomic databases

PaxDbiQ8VHF0.
PRIDEiQ8VHF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170577.
KEGGirno:170577.
UCSCiRGD:619883. rat.

Organism-specific databases

CTDi4140.
RGDi619883. Mark3.

Phylogenomic databases

eggNOGiKOG0586. Eukaryota.
ENOG410XNQ0. LUCA.
HOGENOMiHOG000233025.
HOVERGENiHBG052453.
InParanoidiQ8VHF0.
KOiK08798.
PhylomeDBiQ8VHF0.

Miscellaneous databases

PROiQ8VHF0.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a third microtubule affinity-regulating kinase from rat brain."
    Drewes G.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-374 AND SER-469, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMARK3_RAT
AccessioniPrimary (citable) accession number: Q8VHF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 1, 2002
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.