Reviewed, UniProtKB/Swiss-Prot Q8VHE9 (RETST_RAT)

Names and origin

Protein names

Recommended name:
    All-trans-retinol 13,14-reductase
    EC=1.3.99.23
Alternative name(s):
    All-trans-13,14-dihydroretinol saturase
      Short name=RetSat
    RMT-7

Gene names

Name:	Retsat
Synonyms:	Rmt7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Retinol saturase carrying out the saturation of the 13-14 double bond of all-trans-retinol to produce all-trans-13,14-dihydroretinol. Has activity toward all-trans-retinol as substrate. Does not use all-trans-retinoic acid nor 9-cis, 11-cis or 13-cis-retinol isomers as substrates. May play a role in the metabolism of vitamin A By similarity.
Catalytic activity	All-trans-13,14-dihydroretinol + acceptor = all-trans-retinol + reduced acceptor.
Cofactor	NAD, NADP, or FAD By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein By similarity.
Tissue specificity	Highly expressed in liver, kidney and heart. Ref.1
Induction	Down-regulated in mammary adenocarcinomas. Ref.1
Sequence similarities	Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from sequence or structural similarity. Source: HGNC retinol metabolic process Inferred from sequence or structural similarity. Source: HGNC
Cellular component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: HGNC extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear outer membrane Inferred from sequence or structural similarity. Source: HGNC
Molecular function	all-trans-retinol 13,14-reductase activity Inferred from sequence or structural similarity. Source: HGNC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 609	588	All-trans-retinol 13,14-reductase		PRO_0000225668
Regions
	Nucleotide binding	69 – 97	29	FAD or NAD or NADP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8VHE9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D492DC98E8D23963	FASTA	609	67,531
	10 20 30 40 50 60 MWITALLLVV LLLVVVHRVY VGLFTGSSPN PFAEDVKRPP EPLVTDKEAR KKVLKQAFSV 70 80 90 100 110 120 SRVPEKLDAV VIGSGIGGLA SAAVLAKAGK RVLVLEQHTK AGGCCHTFGE NGLEFDTGIH 130 140 150 160 170 180 YIGRMREGNI GRFILDQITE GQLDWAPMAS PFDLMILEGP NGRKEFPMYS GRKEYIQGLK 190 200 210 220 230 240 EKFPKEEAVI DKYMELVKVV AHGVSHAILL KFLPLPLTQL LNKFGLLTRF SPFCRASTQS 250 260 270 280 290 300 LAEVLKQLGA SPELQAVLSY ILPTYGVTPS HTTFSLHALL VDHYIQGAYY PRRGSSEIAF 310 320 330 340 350 360 HTIPLIQRAG GAVLTRATVQ SVLLDSAGRA CGVSVKKGQE LVNIYCPVVI SNAGMFNTYQ 370 380 390 400 410 420 HLLPESVRYL PDVKKQLTMV KPGLSMLSIF ICLKGTKEEL KLQSTNYYVY FDTDMDKAME 430 440 450 460 470 480 CYVSMPKEKA PEHIPLLFIP FPSSKDPTWE DRFPDRSTMT VLVPTAFEWF EEWQEEPKGK 490 500 510 520 530 540 RGVDYETLKN TFREASMSVI MKLFPQLEGK VESVTGGSPL TNQYYLAAHR GATYGADHDL 550 560 570 580 590 600 ARLHPHAMAS LRAQTPIPNL YLTGQDIFTC GLMGALQGAL LCSSAILKRN LYSDLQALGS KVRAQKKKK

References

[1]

"Altered gene expression profile in chemically induced rat mammary adenocarcinomas and its modulation by an aromatase inhibitor." Wang Y., Hu L., Yao R., Wang M., Crist K.A., Grubbs C.J., Johanning G.L., Lubet R.A., You M. Oncogene 20:7710-7721(2001) [PubMed: 11753649] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: Sprague-Dawley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF465614 mRNA. Translation: AAL73494.1.
IPI	IPI00206948.
RefSeq	NP_659552.1.
UniGene	Rn.55275
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VHE9.
Proteomic databases
PRIDE	Q8VHE9.
Genome annotation databases
Ensembl	ENSRNOT00000019571; ENSRNOP00000019571; ENSRNOG00000014090; Rattus norvegicus. [Genome view]
GeneID	246298.
KEGG	rno:246298.
UCSC	NM_145084. rat.
Organism-specific databases
CTD	246298.
RGD	628802. Retsat.
Phylogenomic databases
HOVERGEN	Q8VHE9.
Enzyme and pathway databases
BRENDA	1.3.99.23. 248.
Gene expression databases
ArrayExpress	Q8VHE9.
Genevestigator	Q8VHE9.
GermOnline	ENSRNOG00000014090. Rattus norvegicus.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
ProDom	PD139017. Phytn_dehydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	623694.

Entry information

Entry name

RETST_RAT

Accession

Primary (citable) accession number: Q8VHE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 1, 2002
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)