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Q8VHC8

- MA2B1_CAVPO

UniProt

Q8VHC8 - MA2B1_CAVPO

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Protein
Lysosomal alpha-mannosidase
Gene
MAN2B1, MANB
Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover By similarity.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Zinc By similarity
Metal bindingi72 – 721Zinc By similarity
Active sitei194 – 1941Nucleophile By similarity
Metal bindingi194 – 1941Zinc By similarity
Metal bindingi444 – 4441Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-mannosidase (EC:3.2.1.24)
Short name:
Laman
Alternative name(s):
Lysosomal acid alpha-mannosidase
Mannosidase alpha class 2B member 1
Mannosidase alpha-B
Gene namesi
Name:MAN2B1
Synonyms:MANB
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in MAN2B1 are the cause of lysosomal alpha-mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains.1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4747 Reviewed prediction
Add
BLAST
Chaini48 – 1007960Lysosomal alpha-mannosidase
PRO_0000012067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 356 By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi266 ↔ 271 By similarity
Glycosylationi308 – 3081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi343 – 3431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi365 – 3651N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi410 ↔ 470 By similarity
Disulfide bondi491 ↔ 499 By similarity
Glycosylationi495 – 4951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi540 – 5401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi639 – 6391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi686 – 6861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi760 – 7601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi927 – 9271N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ8VHC8.
SMRiQ8VHC8. Positions 49-338, 346-419, 429-577, 599-868, 882-1003.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0383.
GeneTreeiENSGT00510000046304.
HOGENOMiHOG000007676.
HOVERGENiHBG052391.
OrthoDBiEOG786H2P.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VHC8-1 [UniParc]FASTAAdd to Basket

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MGASVLPLGL GAGDCQSSSG RRMSACLPRT ALSFLLSLLL ATPGARAAGY     50
ETCPMVQPGM LNVHLVAHTH DDVGWLKTVD QYYWGIHNDL QQAGVQYILD 100
SVISALLAEP TRRFVYVEMA FFSRWWHQQT NETQEVVRRL VRQGRLEFAN 150
GGWVMNDEAA THYGAIVDQM TLGLRFLEDT FGSDGRPRVA WHIDPFGHSR 200
EQASLFAQMG FDGVFFGRID YQDKLVRKKR REMELVWRAS ASLKAPAADL 250
FTGVLPNNYG PPEGLCWDVL CADPPVVDDP RSPEYNAKKL VSYFLQLATA 300
QGRYYRTNHT VMTMGSDFQY ENANTWFKNL DKLIQLVNMQ QANGSRVHVL 350
YSTPACYLWE LNKANLTWPV KEDDFFPYAD GPHMFWTGYF SSRPALKRYE 400
RLSYNFLQVC NQLEAQVGPA ANVGPYGHGD SSPLNQAMAV LQHHDAVSGT 450
SKQHVADDYA RQLAAGWGPC EVLLSNALAK LSGSKETFLF CRDLNISICP 500
FSQTSERFQV LVYNPLGRKV DRMVRLPVRK GLFLIKDPGN NTVPSTVVEL 550
TSSGNPELLF PALVPALGFS VYSVTRVSDQ NPQTRSQHSR PQKYSSPVLS 600
IKNEYLRASF HPDTGLLSMI EVLDRKLTLP VNQAFFWYNA SVGDKRSSQA 650
SGAYIFRPSQ QWPFPVSHLA RTRLVKTALV QEVHQNFTAW CSQVVRLYSG 700
QRHLELEWTV GPIPVGDKWG KEIISRFDTP LETGGVFFTD SNGREVLERR 750
RDYRPSWKLN QTEPVAGNYY PVNSRIYITD GKMQLTVLTD RSQGGSSMSD 800
GSLELMVHRR LLKDDGRGVG EALQEPGSGG WVRGRHLLLL DTAREAAAEH 850
RLLAEKELLA PQLVLAPGQG PSYHHDHHEA VPRKQFSGLR RQLPPSVRLL 900
TLARWGPDTL LLRLEHQFAL GEDSSRNLSL PVTLDLQDLF STFTITRLQE 950
TTLAANQLRA SASRLKWTTE IDPISRPAVP RLDPSSITLQ PMEIRTFVAS 1000
VQWEENS 1007
Length:1,007
Mass (Da):113,184
Last modified:March 1, 2002 - v1
Checksum:iC128EB9948A44D41
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551M → I.1 Publication
Natural varianti227 – 2271R → W in AM. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY036153 mRNA. Translation: AAL58982.1.
AY036154 mRNA. Translation: AAL58983.1.
AY036155 mRNA. Translation: AAL58984.1.
RefSeqiNP_001244175.1. NM_001257246.1.

Genome annotation databases

EnsembliENSCPOT00000024185; ENSCPOP00000017509; ENSCPOG00000002295.
GeneIDi100718205.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY036153 mRNA. Translation: AAL58982.1 .
AY036154 mRNA. Translation: AAL58983.1 .
AY036155 mRNA. Translation: AAL58984.1 .
RefSeqi NP_001244175.1. NM_001257246.1.

3D structure databases

ProteinModelPortali Q8VHC8.
SMRi Q8VHC8. Positions 49-338, 346-419, 429-577, 599-868, 882-1003.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCPOT00000024185 ; ENSCPOP00000017509 ; ENSCPOG00000002295 .
GeneIDi 100718205.

Organism-specific databases

CTDi 4125.

Phylogenomic databases

eggNOGi COG0383.
GeneTreei ENSGT00510000046304.
HOGENOMi HOG000007676.
HOVERGENi HBG052391.
OrthoDBi EOG786H2P.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Alpha-mannosidosis in the guinea pig: cloning of the lysosomal alpha-mannosidase cDNA and identification of a missense mutation causing alpha-mannosidosis."
    Berg T., Hopwood J.J.
    Biochim. Biophys. Acta 1586:169-176(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT AM TRP-227, VARIANT ILE-55.

Entry informationi

Entry nameiMA2B1_CAVPO
AccessioniPrimary (citable) accession number: Q8VHC8
Secondary accession number(s): Q8VHC6, Q8VHC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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