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Protein

Calcium-binding protein 4

Gene

Cabp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in normal synaptic function through regulation of Ca2+ influx and neurotransmitter release in photoreceptor synaptic terminals and in auditory transmission. Modulator of CACNA1D and CACNA1F, suppressing the calcium-dependent inactivation and shifting the activation range to more hyperpolarized voltages.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi138 – 1491PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi215 – 2262PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi252 – 2633PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • photoreceptor cell morphogenesis Source: MGI
  • phototransduction Source: MGI
  • retinal bipolar neuron differentiation Source: MGI
  • retinal cone cell development Source: MGI
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein 4
Short name:
CaBP4
Gene namesi
Name:Cabp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1920910. Cabp4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37S → A: Decreased phosphorylation. 1 Publication1
Mutagenesisi46S → A: No effect on phosphorylation. 1 Publication1
Mutagenesisi50 – 51SS → AA: No effect on phosphorylation. 1 Publication2
Mutagenesisi149E → Q: Loss of calcium binding; when associated with Q-226 and Q-263. 1 Publication1
Mutagenesisi223T → A: No effect on phosphorylation. 1 Publication1
Mutagenesisi226E → Q: Loss of calcium binding; when associated with Q-149 and Q-263. 1 Publication1
Mutagenesisi263E → Q: Loss of calcium binding; when associated with Q-149 and Q-226. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000735221 – 271Calcium-binding protein 4Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei37Phosphoserine; by PKC/PRKCZ1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation levels change with the light conditions and regulate the activity, but has no effect on calcium binding.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VHC5.
PRIDEiQ8VHC5.

PTM databases

iPTMnetiQ8VHC5.
PhosphoSitePlusiQ8VHC5.

Expressioni

Tissue specificityi

Expressed in retina and in the inner hair cells (IHC) of the cochlea.4 Publications

Gene expression databases

BgeeiENSMUSG00000024842.
CleanExiMM_CABP4.
GenevisibleiQ8VHC5. MM.

Interactioni

Subunit structurei

Interacts with CACNA1F and CACNA1D (via IQ domain) in a calcium independent manner. Interacts (via N-terminus) with UNC119.4 Publications

Protein-protein interaction databases

DIPiDIP-60739N.
STRINGi10090.ENSMUSP00000025761.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi124 – 137Combined sources14
Helixi148 – 150Combined sources3
Helixi151 – 155Combined sources5
Turni156 – 158Combined sources3
Helixi164 – 171Combined sources8
Beta strandi174 – 177Combined sources4
Helixi183 – 190Combined sources8
Helixi204 – 214Combined sources11
Helixi224 – 230Combined sources7
Turni233 – 235Combined sources3
Helixi242 – 251Combined sources10
Beta strandi256 – 259Combined sources4
Helixi261 – 268Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M28NMR-A101-271[»]
2M29NMR-A101-271[»]
ProteinModelPortaliQ8VHC5.
SMRiQ8VHC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 160EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini161 – 196EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini202 – 237EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini239 – 271EF-hand 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ8VHC5.
OMAiRELGDCM.
OrthoDBiEOG091G0PB4.
PhylomeDBiQ8VHC5.
TreeFamiTF334804.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR033014. CABP4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR23050:SF196. PTHR23050:SF196. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VHC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATEHNVQLV PGSQKIPKGV VSPRSAAEGP ALTRRRSKKE SWHPGSQKAS
60 70 80 90 100
SGDQSSSQGS EASGSSKHPP RTKVGQEEPS SAPARPASHR HSHRHRSDPQ
110 120 130 140 150
QDAAQRTYGP LLNRMFGKDR ELGPEELEEL QAAFEEFDTD QDGYIGYREL
160 170 180 190 200
GDCMRTLGYM PTEMELLEVS QHVKMRMGGF VDFEEFVELI SPKLREETAH
210 220 230 240 250
MLGVRELRIA FREFDKDRDG RITVAELRQA APALLGEPLE GTELDEMLRE
260 270
MDLNGDGTID FDEFVMMLST G
Length:271
Mass (Da):30,268
Last modified:March 1, 2002 - v1
Checksum:i3B6C7883C3DB8C1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039218 mRNA. Translation: AAK83463.1.
BC049263 mRNA. Translation: AAH49263.1.
CCDSiCCDS29416.1.
RefSeqiNP_653115.1. NM_144532.2.
UniGeneiMm.379226.

Genome annotation databases

EnsembliENSMUST00000025761; ENSMUSP00000025761; ENSMUSG00000024842.
ENSMUST00000181080; ENSMUSP00000137950; ENSMUSG00000097687.
GeneIDi73660.
KEGGimmu:73660.
UCSCiuc008fyw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039218 mRNA. Translation: AAK83463.1.
BC049263 mRNA. Translation: AAH49263.1.
CCDSiCCDS29416.1.
RefSeqiNP_653115.1. NM_144532.2.
UniGeneiMm.379226.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M28NMR-A101-271[»]
2M29NMR-A101-271[»]
ProteinModelPortaliQ8VHC5.
SMRiQ8VHC5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60739N.
STRINGi10090.ENSMUSP00000025761.

PTM databases

iPTMnetiQ8VHC5.
PhosphoSitePlusiQ8VHC5.

Proteomic databases

PaxDbiQ8VHC5.
PRIDEiQ8VHC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025761; ENSMUSP00000025761; ENSMUSG00000024842.
ENSMUST00000181080; ENSMUSP00000137950; ENSMUSG00000097687.
GeneIDi73660.
KEGGimmu:73660.
UCSCiuc008fyw.1. mouse.

Organism-specific databases

CTDi57010.
MGIiMGI:1920910. Cabp4.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ8VHC5.
OMAiRELGDCM.
OrthoDBiEOG091G0PB4.
PhylomeDBiQ8VHC5.
TreeFamiTF334804.

Miscellaneous databases

PROiQ8VHC5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024842.
CleanExiMM_CABP4.
GenevisibleiQ8VHC5. MM.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR033014. CABP4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR23050:SF196. PTHR23050:SF196. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCABP4_MOUSE
AccessioniPrimary (citable) accession number: Q8VHC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.