ID   A6HZ42_RAT              Unreviewed;       149 AA.
AC   A6HZ42; Q8VHC1;
DT   28-JUN-2023, integrated into UniProtKB/TrEMBL.
DT   28-JUN-2023, sequence version 1.
DT   27-MAR-2024, entry version 5.
DE   SubName: Full=Cystatin E/M {ECO:0000313|Ensembl:ENSRNOP00000027727.1};
DE   SubName: Full=Cystatin N {ECO:0000313|EMBL:AAL02328.1};
GN   Name=Cst6 {ECO:0000313|Ensembl:ENSRNOP00000027727.1,
GN   ECO:0000313|RGD:628799};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116 {ECO:0000313|EMBL:AAL02328.1};
RN   [1] {ECO:0000313|EMBL:AAL02328.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Hong J., Rosner M.R.;
RT   "Characterization of a noval cysteine proteinase inhibitor, cystatin N.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSRNOP00000027727.1, ECO:0000313|Proteomes:UP000002494}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000027727.1,
RC   ECO:0000313|Proteomes:UP000002494};
RX   PubMed=15057822; DOI=10.1038/nature02426;
RG   Rat Genome Sequencing Project Consortium;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [3] {ECO:0000313|Ensembl:ENSRNOP00000027727.1}
RP   IDENTIFICATION.
RC   STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000027727.1};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the cystatin family.
CC       {ECO:0000256|ARBA:ARBA00009403}.
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DR   EMBL; AY046415; AAL02328.1; -; mRNA.
DR   RefSeq; NP_598250.1; NM_133566.1.
DR   STRING; 10116.ENSRNOP00000027727; -.
DR   MEROPS; I25.006; -.
DR   PaxDb; 10116-ENSRNOP00000027727; -.
DR   Ensembl; ENSRNOT00000027727.3; ENSRNOP00000027727.1; ENSRNOG00000020455.3.
DR   GeneID; 171096; -.
DR   KEGG; rno:171096; -.
DR   UCSC; RGD:628799; rat.
DR   AGR; RGD:628799; -.
DR   CTD; 1474; -.
DR   RGD; 628799; Cst6.
DR   eggNOG; ENOG502SC50; Eukaryota.
DR   GeneTree; ENSGT00940000161375; -.
DR   HOGENOM; CLU_118168_0_1_1; -.
DR   OMA; ESTECRK; -.
DR   OrthoDB; 3667533at2759; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000020455; Expressed in pancreas and 15 other cell types or tissues.
DR   GO; GO:0001533; C:cornified envelope; ISO:RGD.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0008544; P:epidermis development; ISO:RGD.
DR   CDD; cd00042; CY; 1.
DR   Gene3D; 3.10.450.10; -; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   PANTHER; PTHR47033; CYSTATIN-M; 1.
DR   PANTHER; PTHR47033:SF1; CYSTATIN-M; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; Cystatin/monellin; 1.
PE   2: Evidence at transcript level;
KW   Protease inhibitor {ECO:0000256|ARBA:ARBA00022704};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002494};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP};
KW   Thiol protease inhibitor {ECO:0000256|ARBA:ARBA00022704}.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           29..149
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5041156368"
FT   DOMAIN          33..147
FT                   /note="Cystatin"
FT                   /evidence="ECO:0000259|SMART:SM00043"
SQ   SEQUENCE   149 AA;  16792 MW;  190DD8A89EC133C0 CRC64;
     MERPHFPLAM GLGLLAFCLL TLSPDARAEL RSRRTGERQN LSPDDPRVLK AAQAAVASYN
     MDSNSLYYFR DTKVIDAKCQ LVAGIKYYMT VDIESTECRK TRVSGDHVDL TTCPLAAGVQ
     QEKLRCNFEL LEVPWKNTTQ LLKHDCVQV
//