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Q8VHB5

- CAH9_MOUSE

UniProt

Q8VHB5 - CAH9_MOUSE

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Protein
Carbonic anhydrase 9
Gene
Ca9, Car9
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Reversible hydration of carbon dioxide. Participates in pH regulation By similarity.

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zinc By similarity.

Enzyme regulationi

Inhibited by acetazolamide By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 1791Proton acceptor By similarity
Metal bindingi205 – 2051Zinc; catalytic By similarity
Metal bindingi207 – 2071Zinc; catalytic By similarity
Metal bindingi230 – 2301Zinc; catalytic By similarity

GO - Molecular functioni

  1. carbonate dehydratase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. morphogenesis of an epithelium Source: MGI
  2. one-carbon metabolic process Source: InterPro
  3. response to drug Source: Ensembl
  4. response to hypoxia Source: Ensembl
  5. response to testosterone Source: Ensembl
  6. secretion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_199096. Reversible hydration of carbon dioxide.
REACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 9 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase IX
Carbonic anhydrase IX
Short name:
CA-IX
Short name:
CAIX
Membrane antigen MN homolog
Gene namesi
Name:Ca9
Synonyms:Car9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:2447188. Car9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 390359Extracellular Reviewed prediction
Add
BLAST
Transmembranei391 – 41121Helical; Reviewed prediction
Add
BLAST
Topological domaini412 – 43726Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. microvillus membrane Source: UniProtKB-SubCell
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed prediction
Add
BLAST
Chaini32 – 437406Carbonic anhydrase 9
PRO_0000004244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi98 – 981O-linked (GlcNAc...) By similarity
Disulfide bondi135 ↔ 315 By similarity
Disulfide bondi153 – 153Interchain By similarity
Glycosylationi325 – 3251N-linked (GlcNAc...) By similarity
Modified residuei427 – 4271Phosphotyrosine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ8VHB5.

PTM databases

PhosphoSiteiQ8VHB5.

Expressioni

Gene expression databases

ArrayExpressiQ8VHB5.
BgeeiQ8VHB5.
CleanExiMM_CAR9.
GenevestigatoriQ8VHB5.

Interactioni

Subunit structurei

Forms oligomers linked by disulfide bonds By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030183.

Structurei

3D structure databases

ProteinModelPortaliQ8VHB5.
SMRiQ8VHB5. Positions 118-370.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni32 – 9564Proteoglycan-like (PG) By similarity
Add
BLAST
Regioni96 – 390295Catalytic By similarity
Add
BLAST
Regioni311 – 3122Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3338.
GeneTreeiENSGT00660000095420.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiQ8VHB5.
KOiK01672.
OMAiSRYFRYE.
OrthoDBiEOG7WMCK7.
PhylomeDBiQ8VHB5.
TreeFamiTF316425.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR018429. CA9.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF18. PTHR18952:SF18. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VHB5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASLGPSPWA PLSTPAPTAQ LLLFLLLQVS AQPQGLSGMQ GEPSLGDSSS    50
GEDELGVDVL PSEEDAPEEA DPPDGEDPPE VNSEDRMEES LGLEDLSTPE 100
APEHSQGSHG DEKGGGHSHW SYGGTLLWPQ VSPACAGRFQ SPVDIRLERT 150
AFCRTLQPLE LLGYELQPLP ELSLSNNGHT VQLTLPPGLK MALGPGQEYR 200
ALQLHLHWGT SDHPGSEHTV NGHRFPAEIH VVHLSTAFSE LHEALGRPGG 250
LAVLAAFLQE SPEENSAYEQ LLSHLEEISE EGSKIEIPGL DVSALLPSDL 300
SRYYRYEGSL TTPPCSQGVI WTVFNETVKL SAKQLHTLSV SLWGPRDSRL 350
QLNFRATQPL NGRTIEASFP AAEDSSPEPV HVNSCFTAGD ILALVFGLLF 400
AVTSIAFLLQ LRRQHRHRSG TKDRVSYSPA EMTETGA 437
Length:437
Mass (Da):47,265
Last modified:May 9, 2003 - v2
Checksum:i88F23380DCD35344
GO
Isoform 2 (identifier: Q8VHB5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-282: G → V
     283-437: Missing.

Show »
Length:282
Mass (Da):30,247
Checksum:iFB5E84827B4695AA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei282 – 2821G → V in isoform 2.
VSP_007409
Alternative sequencei283 – 437155Missing in isoform 2.
VSP_007410Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY049077 Genomic DNA. Translation: AAL14193.1.
AJ245857 mRNA. Translation: CAC80975.1.
AB086322 mRNA. Translation: BAC00816.1.
CCDSiCCDS18099.1. [Q8VHB5-1]
RefSeqiNP_647466.2. NM_139305.2. [Q8VHB5-1]
UniGeneiMm.283682.

Genome annotation databases

EnsembliENSMUST00000030183; ENSMUSP00000030183; ENSMUSG00000028463. [Q8VHB5-1]
GeneIDi230099.
KEGGimmu:230099.
UCSCiuc008sqa.1. mouse. [Q8VHB5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY049077 Genomic DNA. Translation: AAL14193.1 .
AJ245857 mRNA. Translation: CAC80975.1 .
AB086322 mRNA. Translation: BAC00816.1 .
CCDSi CCDS18099.1. [Q8VHB5-1 ]
RefSeqi NP_647466.2. NM_139305.2. [Q8VHB5-1 ]
UniGenei Mm.283682.

3D structure databases

ProteinModelPortali Q8VHB5.
SMRi Q8VHB5. Positions 118-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000030183.

PTM databases

PhosphoSitei Q8VHB5.

Proteomic databases

PRIDEi Q8VHB5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030183 ; ENSMUSP00000030183 ; ENSMUSG00000028463 . [Q8VHB5-1 ]
GeneIDi 230099.
KEGGi mmu:230099.
UCSCi uc008sqa.1. mouse. [Q8VHB5-1 ]

Organism-specific databases

CTDi 230099.
MGIi MGI:2447188. Car9.

Phylogenomic databases

eggNOGi COG3338.
GeneTreei ENSGT00660000095420.
HOGENOMi HOG000112637.
HOVERGENi HBG002837.
InParanoidi Q8VHB5.
KOi K01672.
OMAi SRYFRYE.
OrthoDBi EOG7WMCK7.
PhylomeDBi Q8VHB5.
TreeFami TF316425.

Enzyme and pathway databases

Reactomei REACT_199096. Reversible hydration of carbon dioxide.
REACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.

Miscellaneous databases

NextBioi 379788.
PROi Q8VHB5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8VHB5.
Bgeei Q8VHB5.
CleanExi MM_CAR9.
Genevestigatori Q8VHB5.

Family and domain databases

Gene3Di 3.10.200.10. 1 hit.
InterProi IPR018429. CA9.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view ]
PANTHERi PTHR18952. PTHR18952. 1 hit.
PTHR18952:SF18. PTHR18952:SF18. 1 hit.
Pfami PF00194. Carb_anhydrase. 1 hit.
[Graphical view ]
SMARTi SM01057. Carb_anhydrase. 1 hit.
[Graphical view ]
SUPFAMi SSF51069. SSF51069. 1 hit.
PROSITEi PS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Monoclonal antibodies generated in carbonic anhydrase IX-deficient mice recognize different domains of tumour-associated hypoxia-induced carbonic anhydrase IX."
    Zat'ovicova M., Tarabkova K., Svastova E., Gibadulinova A., Mucha V., Jakubickova L., Biesova Z., Rafajova M., Ortova Gut M.O., Parkkila S., Parkkila A.-K., Waheed A., Sly W.S., Horak I., Pastorek J., Pastorekova S.
    J. Immunol. Methods 282:117-134(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    Strain: 129/Ola.
  2. Ortova M.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Alternative spliced mRNA coding for MN/CA9."
    Wang Y.P., Yoshikawa K., Kozaki K., Miyaishi O., Nakagawa A., Muramatsu H., Kawada Y., Uchida K., Nishikawa N., Saga S.
    Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: ICR.
    Tissue: Small intestine.

Entry informationi

Entry nameiCAH9_MOUSE
AccessioniPrimary (citable) accession number: Q8VHB5
Secondary accession number(s): Q8K1G1, Q8VDE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 9, 2003
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi