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Protein

Carbonic anhydrase 9

Gene

Ca9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Reversible hydration of carbon dioxide. Participates in pH regulation (By similarity).By similarity

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zn2+By similarity

Enzyme regulationi

Inhibited by acetazolamide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei179Proton acceptorBy similarity1
Metal bindingi205Zinc; catalyticBy similarity1
Metal bindingi207Zinc; catalyticBy similarity1
Metal bindingi230Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • morphogenesis of an epithelium Source: MGI
  • one-carbon metabolic process Source: InterPro
  • response to drug Source: Ensembl
  • response to hypoxia Source: Ensembl
  • response to testosterone Source: Ensembl
  • secretion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1475029. Reversible hydration of carbon dioxide.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 9 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase IX
Carbonic anhydrase IX
Short name:
CA-IX
Short name:
CAIX
Membrane antigen MN homolog
Gene namesi
Name:Ca9
Synonyms:Car9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2447188. Car9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 390ExtracellularSequence analysisAdd BLAST359
Transmembranei391 – 411HelicalSequence analysisAdd BLAST21
Topological domaini412 – 437CytoplasmicSequence analysisAdd BLAST26

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000000424432 – 437Carbonic anhydrase 9Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi98O-linked (GlcNAc...)By similarity1
Disulfide bondi135 ↔ 315By similarity
Disulfide bondi153InterchainBy similarity
Glycosylationi325N-linked (GlcNAc...)By similarity1
Modified residuei427PhosphotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8VHB5.
PRIDEiQ8VHB5.

PTM databases

PhosphoSitePlusiQ8VHB5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028463.
CleanExiMM_CAR9.
ExpressionAtlasiQ8VHB5. baseline and differential.
GenevisibleiQ8VHB5. MM.

Interactioni

Subunit structurei

Forms oligomers linked by disulfide bonds.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030183.

Structurei

3D structure databases

ProteinModelPortaliQ8VHB5.
SMRiQ8VHB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini118 – 369Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 95Proteoglycan-like (PG)By similarityAdd BLAST64
Regioni96 – 390CatalyticBy similarityAdd BLAST295
Regioni311 – 312Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the alpha-carbonic anhydrase family.Curated
Contains 1 alpha-carbonic anhydrase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0382. Eukaryota.
COG3338. LUCA.
GeneTreeiENSGT00760000118915.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiQ8VHB5.
KOiK01672.
OMAiSRYFRYE.
OrthoDBiEOG091G0XFM.
PhylomeDBiQ8VHB5.
TreeFamiTF316425.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR018429. CA9.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF18. PTHR18952:SF18. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VHB5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLGPSPWA PLSTPAPTAQ LLLFLLLQVS AQPQGLSGMQ GEPSLGDSSS
60 70 80 90 100
GEDELGVDVL PSEEDAPEEA DPPDGEDPPE VNSEDRMEES LGLEDLSTPE
110 120 130 140 150
APEHSQGSHG DEKGGGHSHW SYGGTLLWPQ VSPACAGRFQ SPVDIRLERT
160 170 180 190 200
AFCRTLQPLE LLGYELQPLP ELSLSNNGHT VQLTLPPGLK MALGPGQEYR
210 220 230 240 250
ALQLHLHWGT SDHPGSEHTV NGHRFPAEIH VVHLSTAFSE LHEALGRPGG
260 270 280 290 300
LAVLAAFLQE SPEENSAYEQ LLSHLEEISE EGSKIEIPGL DVSALLPSDL
310 320 330 340 350
SRYYRYEGSL TTPPCSQGVI WTVFNETVKL SAKQLHTLSV SLWGPRDSRL
360 370 380 390 400
QLNFRATQPL NGRTIEASFP AAEDSSPEPV HVNSCFTAGD ILALVFGLLF
410 420 430
AVTSIAFLLQ LRRQHRHRSG TKDRVSYSPA EMTETGA
Length:437
Mass (Da):47,265
Last modified:May 9, 2003 - v2
Checksum:i88F23380DCD35344
GO
Isoform 2 (identifier: Q8VHB5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-282: G → V
     283-437: Missing.

Show »
Length:282
Mass (Da):30,247
Checksum:iFB5E84827B4695AA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007409282G → V in isoform 2. 1 Publication1
Alternative sequenceiVSP_007410283 – 437Missing in isoform 2. 1 PublicationAdd BLAST155

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049077 Genomic DNA. Translation: AAL14193.1.
AJ245857 mRNA. Translation: CAC80975.1.
AB086322 mRNA. Translation: BAC00816.1.
CCDSiCCDS18099.1. [Q8VHB5-1]
RefSeqiNP_647466.2. NM_139305.2. [Q8VHB5-1]
UniGeneiMm.283682.

Genome annotation databases

EnsembliENSMUST00000030183; ENSMUSP00000030183; ENSMUSG00000028463. [Q8VHB5-1]
GeneIDi230099.
KEGGimmu:230099.
UCSCiuc008sqa.1. mouse. [Q8VHB5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049077 Genomic DNA. Translation: AAL14193.1.
AJ245857 mRNA. Translation: CAC80975.1.
AB086322 mRNA. Translation: BAC00816.1.
CCDSiCCDS18099.1. [Q8VHB5-1]
RefSeqiNP_647466.2. NM_139305.2. [Q8VHB5-1]
UniGeneiMm.283682.

3D structure databases

ProteinModelPortaliQ8VHB5.
SMRiQ8VHB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030183.

PTM databases

PhosphoSitePlusiQ8VHB5.

Proteomic databases

PaxDbiQ8VHB5.
PRIDEiQ8VHB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030183; ENSMUSP00000030183; ENSMUSG00000028463. [Q8VHB5-1]
GeneIDi230099.
KEGGimmu:230099.
UCSCiuc008sqa.1. mouse. [Q8VHB5-1]

Organism-specific databases

CTDi230099.
MGIiMGI:2447188. Car9.

Phylogenomic databases

eggNOGiKOG0382. Eukaryota.
COG3338. LUCA.
GeneTreeiENSGT00760000118915.
HOGENOMiHOG000112637.
HOVERGENiHBG002837.
InParanoidiQ8VHB5.
KOiK01672.
OMAiSRYFRYE.
OrthoDBiEOG091G0XFM.
PhylomeDBiQ8VHB5.
TreeFamiTF316425.

Enzyme and pathway databases

ReactomeiR-MMU-1475029. Reversible hydration of carbon dioxide.

Miscellaneous databases

PROiQ8VHB5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028463.
CleanExiMM_CAR9.
ExpressionAtlasiQ8VHB5. baseline and differential.
GenevisibleiQ8VHB5. MM.

Family and domain databases

Gene3Di3.10.200.10. 1 hit.
InterProiIPR018429. CA9.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view]
PANTHERiPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF18. PTHR18952:SF18. 1 hit.
PfamiPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTiSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMiSSF51069. SSF51069. 1 hit.
PROSITEiPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAH9_MOUSE
AccessioniPrimary (citable) accession number: Q8VHB5
Secondary accession number(s): Q8K1G1, Q8VDE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 9, 2003
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.