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Q8VHB5 (CAH9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbonic anhydrase 9

EC=4.2.1.1
Alternative name(s):
Carbonate dehydratase IX
Carbonic anhydrase IX
Short name=CA-IX
Short name=CAIX
Membrane antigen MN homolog
Gene names
Name:Ca9
Synonyms:Car9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Reversible hydration of carbon dioxide. Participates in pH regulation By similarity.

Catalytic activity

H2CO3 = CO2 + H2O.

Cofactor

Zinc By similarity.

Enzyme regulation

Inhibited by acetazolamide By similarity.

Subunit structure

Forms oligomers linked by disulfide bonds By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Cell projectionmicrovillus membrane; Single-pass type I membrane protein By similarity.

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VHB5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VHB5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     282-282: G → V
     283-437: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 437406Carbonic anhydrase 9
PRO_0000004244

Regions

Topological domain32 – 390359Extracellular Potential
Transmembrane391 – 41121Helical; Potential
Topological domain412 – 43726Cytoplasmic Potential
Region32 – 9564Proteoglycan-like (PG) By similarity
Region96 – 390295Catalytic By similarity
Region311 – 3122Substrate binding By similarity

Sites

Active site1791Proton acceptor By similarity
Metal binding2051Zinc; catalytic By similarity
Metal binding2071Zinc; catalytic By similarity
Metal binding2301Zinc; catalytic By similarity

Amino acid modifications

Modified residue4271Phosphotyrosine By similarity
Glycosylation981O-linked (GlcNAc...) By similarity
Glycosylation3251N-linked (GlcNAc...) By similarity
Disulfide bond135 ↔ 315 By similarity
Disulfide bond153Interchain By similarity

Natural variations

Alternative sequence2821G → V in isoform 2.
VSP_007409
Alternative sequence283 – 437155Missing in isoform 2.
VSP_007410

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 9, 2003. Version 2.
Checksum: 88F23380DCD35344

FASTA43747,265
        10         20         30         40         50         60 
MASLGPSPWA PLSTPAPTAQ LLLFLLLQVS AQPQGLSGMQ GEPSLGDSSS GEDELGVDVL 

        70         80         90        100        110        120 
PSEEDAPEEA DPPDGEDPPE VNSEDRMEES LGLEDLSTPE APEHSQGSHG DEKGGGHSHW 

       130        140        150        160        170        180 
SYGGTLLWPQ VSPACAGRFQ SPVDIRLERT AFCRTLQPLE LLGYELQPLP ELSLSNNGHT 

       190        200        210        220        230        240 
VQLTLPPGLK MALGPGQEYR ALQLHLHWGT SDHPGSEHTV NGHRFPAEIH VVHLSTAFSE 

       250        260        270        280        290        300 
LHEALGRPGG LAVLAAFLQE SPEENSAYEQ LLSHLEEISE EGSKIEIPGL DVSALLPSDL 

       310        320        330        340        350        360 
SRYYRYEGSL TTPPCSQGVI WTVFNETVKL SAKQLHTLSV SLWGPRDSRL QLNFRATQPL 

       370        380        390        400        410        420 
NGRTIEASFP AAEDSSPEPV HVNSCFTAGD ILALVFGLLF AVTSIAFLLQ LRRQHRHRSG 

       430 
TKDRVSYSPA EMTETGA 

« Hide

Isoform 2 [UniParc].

Checksum: FB5E84827B4695AA
Show »

FASTA28230,247

References

[1]"Monoclonal antibodies generated in carbonic anhydrase IX-deficient mice recognize different domains of tumour-associated hypoxia-induced carbonic anhydrase IX."
Zat'ovicova M., Tarabkova K., Svastova E., Gibadulinova A., Mucha V., Jakubickova L., Biesova Z., Rafajova M., Ortova Gut M.O., Parkkila S., Parkkila A.-K., Waheed A., Sly W.S., Horak I., Pastorek J., Pastorekova S.
J. Immunol. Methods 282:117-134(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
Strain: 129/Ola.
[2]Ortova M.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Alternative spliced mRNA coding for MN/CA9."
Wang Y.P., Yoshikawa K., Kozaki K., Miyaishi O., Nakagawa A., Muramatsu H., Kawada Y., Uchida K., Nishikawa N., Saga S.
Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: ICR.
Tissue: Small intestine.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY049077 Genomic DNA. Translation: AAL14193.1.
AJ245857 mRNA. Translation: CAC80975.1.
AB086322 mRNA. Translation: BAC00816.1.
CCDSCCDS18099.1. [Q8VHB5-1]
RefSeqNP_647466.2. NM_139305.2. [Q8VHB5-1]
UniGeneMm.283682.

3D structure databases

ProteinModelPortalQ8VHB5.
SMRQ8VHB5. Positions 118-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000030183.

PTM databases

PhosphoSiteQ8VHB5.

Proteomic databases

PRIDEQ8VHB5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030183; ENSMUSP00000030183; ENSMUSG00000028463. [Q8VHB5-1]
GeneID230099.
KEGGmmu:230099.
UCSCuc008sqa.1. mouse. [Q8VHB5-1]

Organism-specific databases

CTD230099.
MGIMGI:2447188. Car9.

Phylogenomic databases

eggNOGCOG3338.
GeneTreeENSGT00660000095420.
HOGENOMHOG000112637.
HOVERGENHBG002837.
InParanoidQ8VHB5.
KOK01672.
OMASRYFRYE.
OrthoDBEOG7WMCK7.
PhylomeDBQ8VHB5.
TreeFamTF316425.

Gene expression databases

ArrayExpressQ8VHB5.
BgeeQ8VHB5.
CleanExMM_CAR9.
GenevestigatorQ8VHB5.

Family and domain databases

Gene3D3.10.200.10. 1 hit.
InterProIPR018429. CA9.
IPR001148. Carbonic_anhydrase_a.
IPR023561. Carbonic_anhydrase_a-class.
IPR018338. Carbonic_anhydrase_a-class_CS.
[Graphical view]
PANTHERPTHR18952. PTHR18952. 1 hit.
PTHR18952:SF18. PTHR18952:SF18. 1 hit.
PfamPF00194. Carb_anhydrase. 1 hit.
[Graphical view]
SMARTSM01057. Carb_anhydrase. 1 hit.
[Graphical view]
SUPFAMSSF51069. SSF51069. 1 hit.
PROSITEPS00162. ALPHA_CA_1. 1 hit.
PS51144. ALPHA_CA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio379788.
PROQ8VHB5.
SOURCESearch...

Entry information

Entry nameCAH9_MOUSE
AccessionPrimary (citable) accession number: Q8VHB5
Secondary accession number(s): Q8K1G1, Q8VDE4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 9, 2003
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot