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Q8VHB5

- CAH9_MOUSE

UniProt

Q8VHB5 - CAH9_MOUSE

Protein

Carbonic anhydrase 9

Gene

Ca9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (09 May 2003)
      Previous versions | rss
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    Functioni

    Reversible hydration of carbon dioxide. Participates in pH regulation By similarity.By similarity

    Catalytic activityi

    H2CO3 = CO2 + H2O.

    Cofactori

    Zinc.By similarity

    Enzyme regulationi

    Inhibited by acetazolamide.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei179 – 1791Proton acceptorBy similarity
    Metal bindingi205 – 2051Zinc; catalyticBy similarity
    Metal bindingi207 – 2071Zinc; catalyticBy similarity
    Metal bindingi230 – 2301Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. carbonate dehydratase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. morphogenesis of an epithelium Source: MGI
    2. one-carbon metabolic process Source: InterPro
    3. response to drug Source: Ensembl
    4. response to hypoxia Source: Ensembl
    5. response to testosterone Source: Ensembl
    6. secretion Source: MGI

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199096. Reversible hydration of carbon dioxide.
    REACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbonic anhydrase 9 (EC:4.2.1.1)
    Alternative name(s):
    Carbonate dehydratase IX
    Carbonic anhydrase IX
    Short name:
    CA-IX
    Short name:
    CAIX
    Membrane antigen MN homolog
    Gene namesi
    Name:Ca9
    Synonyms:Car9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2447188. Car9.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. microvillus membrane Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 437406Carbonic anhydrase 9PRO_0000004244Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi98 – 981O-linked (GlcNAc...)By similarity
    Disulfide bondi135 ↔ 315By similarity
    Disulfide bondi153 – 153InterchainBy similarity
    Glycosylationi325 – 3251N-linked (GlcNAc...)By similarity
    Modified residuei427 – 4271PhosphotyrosineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ8VHB5.

    PTM databases

    PhosphoSiteiQ8VHB5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8VHB5.
    BgeeiQ8VHB5.
    CleanExiMM_CAR9.
    GenevestigatoriQ8VHB5.

    Interactioni

    Subunit structurei

    Forms oligomers linked by disulfide bonds.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000030183.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VHB5.
    SMRiQ8VHB5. Positions 118-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 390359ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini412 – 43726CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei391 – 41121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni32 – 9564Proteoglycan-like (PG)By similarityAdd
    BLAST
    Regioni96 – 390295CatalyticBy similarityAdd
    BLAST
    Regioni311 – 3122Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the alpha-carbonic anhydrase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3338.
    GeneTreeiENSGT00660000095420.
    HOGENOMiHOG000112637.
    HOVERGENiHBG002837.
    InParanoidiQ8VHB5.
    KOiK01672.
    OMAiSRYFRYE.
    OrthoDBiEOG7WMCK7.
    PhylomeDBiQ8VHB5.
    TreeFamiTF316425.

    Family and domain databases

    Gene3Di3.10.200.10. 1 hit.
    InterProiIPR018429. CA9.
    IPR001148. Carbonic_anhydrase_a.
    IPR023561. Carbonic_anhydrase_a-class.
    IPR018338. Carbonic_anhydrase_a-class_CS.
    [Graphical view]
    PANTHERiPTHR18952. PTHR18952. 1 hit.
    PTHR18952:SF18. PTHR18952:SF18. 1 hit.
    PfamiPF00194. Carb_anhydrase. 1 hit.
    [Graphical view]
    SMARTiSM01057. Carb_anhydrase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51069. SSF51069. 1 hit.
    PROSITEiPS00162. ALPHA_CA_1. 1 hit.
    PS51144. ALPHA_CA_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VHB5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASLGPSPWA PLSTPAPTAQ LLLFLLLQVS AQPQGLSGMQ GEPSLGDSSS    50
    GEDELGVDVL PSEEDAPEEA DPPDGEDPPE VNSEDRMEES LGLEDLSTPE 100
    APEHSQGSHG DEKGGGHSHW SYGGTLLWPQ VSPACAGRFQ SPVDIRLERT 150
    AFCRTLQPLE LLGYELQPLP ELSLSNNGHT VQLTLPPGLK MALGPGQEYR 200
    ALQLHLHWGT SDHPGSEHTV NGHRFPAEIH VVHLSTAFSE LHEALGRPGG 250
    LAVLAAFLQE SPEENSAYEQ LLSHLEEISE EGSKIEIPGL DVSALLPSDL 300
    SRYYRYEGSL TTPPCSQGVI WTVFNETVKL SAKQLHTLSV SLWGPRDSRL 350
    QLNFRATQPL NGRTIEASFP AAEDSSPEPV HVNSCFTAGD ILALVFGLLF 400
    AVTSIAFLLQ LRRQHRHRSG TKDRVSYSPA EMTETGA 437
    Length:437
    Mass (Da):47,265
    Last modified:May 9, 2003 - v2
    Checksum:i88F23380DCD35344
    GO
    Isoform 2 (identifier: Q8VHB5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         282-282: G → V
         283-437: Missing.

    Show »
    Length:282
    Mass (Da):30,247
    Checksum:iFB5E84827B4695AA
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei282 – 2821G → V in isoform 2. 1 PublicationVSP_007409
    Alternative sequencei283 – 437155Missing in isoform 2. 1 PublicationVSP_007410Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY049077 Genomic DNA. Translation: AAL14193.1.
    AJ245857 mRNA. Translation: CAC80975.1.
    AB086322 mRNA. Translation: BAC00816.1.
    CCDSiCCDS18099.1. [Q8VHB5-1]
    RefSeqiNP_647466.2. NM_139305.2. [Q8VHB5-1]
    UniGeneiMm.283682.

    Genome annotation databases

    EnsembliENSMUST00000030183; ENSMUSP00000030183; ENSMUSG00000028463. [Q8VHB5-1]
    GeneIDi230099.
    KEGGimmu:230099.
    UCSCiuc008sqa.1. mouse. [Q8VHB5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY049077 Genomic DNA. Translation: AAL14193.1 .
    AJ245857 mRNA. Translation: CAC80975.1 .
    AB086322 mRNA. Translation: BAC00816.1 .
    CCDSi CCDS18099.1. [Q8VHB5-1 ]
    RefSeqi NP_647466.2. NM_139305.2. [Q8VHB5-1 ]
    UniGenei Mm.283682.

    3D structure databases

    ProteinModelPortali Q8VHB5.
    SMRi Q8VHB5. Positions 118-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000030183.

    PTM databases

    PhosphoSitei Q8VHB5.

    Proteomic databases

    PRIDEi Q8VHB5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030183 ; ENSMUSP00000030183 ; ENSMUSG00000028463 . [Q8VHB5-1 ]
    GeneIDi 230099.
    KEGGi mmu:230099.
    UCSCi uc008sqa.1. mouse. [Q8VHB5-1 ]

    Organism-specific databases

    CTDi 230099.
    MGIi MGI:2447188. Car9.

    Phylogenomic databases

    eggNOGi COG3338.
    GeneTreei ENSGT00660000095420.
    HOGENOMi HOG000112637.
    HOVERGENi HBG002837.
    InParanoidi Q8VHB5.
    KOi K01672.
    OMAi SRYFRYE.
    OrthoDBi EOG7WMCK7.
    PhylomeDBi Q8VHB5.
    TreeFami TF316425.

    Enzyme and pathway databases

    Reactomei REACT_199096. Reversible hydration of carbon dioxide.
    REACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.

    Miscellaneous databases

    NextBioi 379788.
    PROi Q8VHB5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VHB5.
    Bgeei Q8VHB5.
    CleanExi MM_CAR9.
    Genevestigatori Q8VHB5.

    Family and domain databases

    Gene3Di 3.10.200.10. 1 hit.
    InterProi IPR018429. CA9.
    IPR001148. Carbonic_anhydrase_a.
    IPR023561. Carbonic_anhydrase_a-class.
    IPR018338. Carbonic_anhydrase_a-class_CS.
    [Graphical view ]
    PANTHERi PTHR18952. PTHR18952. 1 hit.
    PTHR18952:SF18. PTHR18952:SF18. 1 hit.
    Pfami PF00194. Carb_anhydrase. 1 hit.
    [Graphical view ]
    SMARTi SM01057. Carb_anhydrase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51069. SSF51069. 1 hit.
    PROSITEi PS00162. ALPHA_CA_1. 1 hit.
    PS51144. ALPHA_CA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Monoclonal antibodies generated in carbonic anhydrase IX-deficient mice recognize different domains of tumour-associated hypoxia-induced carbonic anhydrase IX."
      Zat'ovicova M., Tarabkova K., Svastova E., Gibadulinova A., Mucha V., Jakubickova L., Biesova Z., Rafajova M., Ortova Gut M.O., Parkkila S., Parkkila A.-K., Waheed A., Sly W.S., Horak I., Pastorek J., Pastorekova S.
      J. Immunol. Methods 282:117-134(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
      Strain: 129/Ola.
    2. Ortova M.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Alternative spliced mRNA coding for MN/CA9."
      Wang Y.P., Yoshikawa K., Kozaki K., Miyaishi O., Nakagawa A., Muramatsu H., Kawada Y., Uchida K., Nishikawa N., Saga S.
      Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: ICR.
      Tissue: Small intestine.

    Entry informationi

    Entry nameiCAH9_MOUSE
    AccessioniPrimary (citable) accession number: Q8VHB5
    Secondary accession number(s): Q8K1G1, Q8VDE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 9, 2003
    Last sequence update: May 9, 2003
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3