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Protein

Notchless protein homolog 1

Gene

Nle1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in regulating Notch activity (Probable). Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival.Curated2 Publications

GO - Biological processi

  • hematopoietic stem cell homeostasis Source: MGI
  • inner cell mass cell differentiation Source: MGI
  • kidney development Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: MGI
  • negative regulation of mitotic cell cycle Source: MGI
  • Notch signaling pathway Source: MGI
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • ribosomal large subunit biogenesis Source: MGI
  • skeletal system morphogenesis Source: MGI
  • somitogenesis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Notch signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Notchless protein homolog 1
Gene namesi
Name:Nle1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2429770. Nle1.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality prior to E6.5 with death of inner mass cells. Embryos implant but die shortly after.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi163 – 1631S → P in l11Jus4; embryos survive through implantation but fail prior to gastrulation. 1 Publication
Mutagenesisi396 – 3961I → S in l11Jus1; embryos survive through implantation but fail prior to gastrulation. Cdkna1 expression is up-regulated while expression of several Wnt pathway members is down-regulated. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 485484Notchless protein homolog 1PRO_0000051099Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei79 – 791PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VEJ4.
MaxQBiQ8VEJ4.
PaxDbiQ8VEJ4.
PeptideAtlasiQ8VEJ4.
PRIDEiQ8VEJ4.

PTM databases

iPTMnetiQ8VEJ4.
PhosphoSiteiQ8VEJ4.

Expressioni

Tissue specificityi

Highest expression in heart, brain, lung, liver, skeletal muscle and testis with lower levels in spleen and kidney.1 Publication

Developmental stagei

Expression detected in the embryo at E7.0, E11.0 and E15.0 and also in the adult.1 Publication

Gene expression databases

BgeeiQ8VEJ4.
CleanExiMM_NLE1.
ExpressionAtlasiQ8VEJ4. baseline and differential.
GenevisibleiQ8VEJ4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8VEJ4. 1 interaction.
MINTiMINT-4098180.
STRINGi10090.ENSMUSP00000099502.

Structurei

3D structure databases

ProteinModelPortaliQ8VEJ4.
SMRiQ8VEJ4. Positions 35-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati112 – 15140WD 1Add
BLAST
Repeati154 – 19340WD 2Add
BLAST
Repeati197 – 24145WD 3Add
BLAST
Repeati244 – 28239WD 4Add
BLAST
Repeati327 – 36640WD 5Add
BLAST
Repeati370 – 40940WD 6Add
BLAST
Repeati412 – 45140WD 7Add
BLAST
Repeati454 – 48431WD 8Add
BLAST

Sequence similaritiesi

Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0271. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00830000128308.
HOGENOMiHOG000091641.
HOVERGENiHBG007602.
InParanoidiQ8VEJ4.
KOiK14855.
OMAiHINCLAW.
OrthoDBiEOG7SR4M7.
TreeFamiTF300668.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VEJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVVEEAA AGDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDKLQLVCNA
60 70 80 90 100
LLAQEEPLPL AFYVHDAEIV SSLGKTLESQ SVETEKIVDI IYQPQAVFRV
110 120 130 140 150
RAVTRCTSSL EGHSEAVISV AFSPTGKYLA SGSGDTTVRF WDLSTETPHF
160 170 180 190 200
TCKGHRHWVL SISWSPDGKK LASGCKNGQV LLWDPSTGLQ VGRTLTGHSK
210 220 230 240 250
WITGLSWEPL HMNPECRYVA SSSKDGSVRV WDTTAGRCER ILTGHTQSVT
260 270 280 290 300
CLRWGGDGLL YSASQDRTIK VWRAHDGVLC RTLQGHGHWV NTMALSTDYA
310 320 330 340 350
LRTGAFEPAE ATVNAQDLQG SLKELKERAS SRYNLVRGQG PERLVSGSDD
360 370 380 390 400
FTLFLWSPAE DKKPLARMTG HQALINQVLF SPDSRIVASA SFDKSIKLWD
410 420 430 440 450
GRTGKYLASL RGHVAAVYQI AWSADSRLLV SGSSDSTLKV WDVKAQKLAT
460 470 480
DLPGHADEVY AVDWSPDGQR VASGGKDKCL RIWRR
Length:485
Mass (Da):53,134
Last modified:July 27, 2011 - v4
Checksum:iD0E7402BB0F44416
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801V → I in AAH18399 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603745 Genomic DNA. Translation: CAI25495.1.
BC018399 mRNA. Translation: AAH18399.1.
CCDSiCCDS25152.1.
RefSeqiNP_663406.2. NM_145431.2.
UniGeneiMm.29176.

Genome annotation databases

EnsembliENSMUST00000103213; ENSMUSP00000099502; ENSMUSG00000020692.
GeneIDi217011.
KEGGimmu:217011.
UCSCiuc007knt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603745 Genomic DNA. Translation: CAI25495.1.
BC018399 mRNA. Translation: AAH18399.1.
CCDSiCCDS25152.1.
RefSeqiNP_663406.2. NM_145431.2.
UniGeneiMm.29176.

3D structure databases

ProteinModelPortaliQ8VEJ4.
SMRiQ8VEJ4. Positions 35-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VEJ4. 1 interaction.
MINTiMINT-4098180.
STRINGi10090.ENSMUSP00000099502.

PTM databases

iPTMnetiQ8VEJ4.
PhosphoSiteiQ8VEJ4.

Proteomic databases

EPDiQ8VEJ4.
MaxQBiQ8VEJ4.
PaxDbiQ8VEJ4.
PeptideAtlasiQ8VEJ4.
PRIDEiQ8VEJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103213; ENSMUSP00000099502; ENSMUSG00000020692.
GeneIDi217011.
KEGGimmu:217011.
UCSCiuc007knt.2. mouse.

Organism-specific databases

CTDi54475.
MGIiMGI:2429770. Nle1.

Phylogenomic databases

eggNOGiKOG0271. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00830000128308.
HOGENOMiHOG000091641.
HOVERGENiHBG007602.
InParanoidiQ8VEJ4.
KOiK14855.
OMAiHINCLAW.
OrthoDBiEOG7SR4M7.
TreeFamiTF300668.

Miscellaneous databases

PROiQ8VEJ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VEJ4.
CleanExiMM_NLE1.
ExpressionAtlasiQ8VEJ4. baseline and differential.
GenevisibleiQ8VEJ4. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Lung.
  3. "The murine ortholog of notchless, a direct regulator of the notch pathway in Drosophila melanogaster, is essential for survival of inner cell mass cells."
    Cormier S., Le Bras S., Souilhol C., Vandormael-Pournin S., Durand B., Babinet C., Baldacci P., Cohen-Tannoudji M.
    Mol. Cell. Biol. 26:3541-3549(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Pancreas and Spleen.
  5. "ENU mutagenesis reveals that Notchless homolog 1 (Drosophila) affects Cdkn1a and several members of the Wnt pathway during murine pre-implantation development."
    Lossie A.C., Lo C.L., Baumgarner K.M., Cramer M.J., Garner J.P., Justice M.J.
    BMC Genet. 13:106-106(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-163 AND ILE-396.
    Strain: C57BL/6J.

Entry informationi

Entry nameiNLE1_MOUSE
AccessioniPrimary (citable) accession number: Q8VEJ4
Secondary accession number(s): B1ARD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 113 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.