Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hyaluronidase-3

Gene

Hyal3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.35. 3474.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-3 (EC:3.2.1.35)
Short name:
Hyal-3
Alternative name(s):
Hyaluronoglucosaminidase-3
Gene namesi
Name:Hyal3
Synonyms:Hyl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1330288. Hyal3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 412390Hyaluronidase-3PRO_0000248201Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 332By similarity
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi206 ↔ 221By similarity
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi357 ↔ 368By similarity
Disulfide bondi362 ↔ 396By similarity
Disulfide bondi398 ↔ 407By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8VEI3.

Expressioni

Tissue specificityi

Liver, kidney, skin, brain, stomach and testis.1 Publication

Gene expression databases

BgeeiQ8VEI3.
CleanExiMM_HYAL3.
ExpressionAtlasiQ8VEI3. baseline and differential.
GenevisibleiQ8VEI3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042667.

Structurei

3D structure databases

ProteinModelPortaliQ8VEI3.
SMRiQ8VEI3. Positions 26-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini353 – 40856EGF-likeAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiNOG69101.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ8VEI3.
KOiK01197.
OMAiWEEWCPL.
OrthoDBiEOG74J97S.
PhylomeDBiQ8VEI3.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR027260. Hyaluronidase-3.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PTHR11769:SF19. PTHR11769:SF19. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500776. Hyaluronidase_3. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VEI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMHLGLMMV VGLTLCLMHG QALLQVPEHP FSVVWNVPSA RCKAHFGVHL
60 70 80 90 100
PLDALGIVAN HGQHFHGQNI SIFYKNQFGL YPYFGPRGTA HNGGIPQAVS
110 120 130 140 150
LDHHLARAAH QILHSLGSSF AGLAVLDWEE WYPLWAGNWG PHRQVYLAAS
160 170 180 190 200
WVWTQQMFPG LDPQEQLHKA HTSFEQAARA LMEYTLQLGR TLRPSGLWGF
210 220 230 240 250
YRYPACGNGW HKMASNYTGH CHAAITTRNT QLRWLWAASS ALFPSIYLPP
260 270 280 290 300
RLPLAYRQAF VRHRLEEAFR VALLEHSHPL PVLAYSRLTH RSSGRFLSLD
310 320 330 340 350
DLMQTIGVSA ALGTAGVVLW GDLSFSSSEE KCWRLHDYLV GTLGPYVINV
360 370 380 390 400
TKAAMACSHQ RCHGHGRCAR KDPGQMEAFL HLQPDDSLGA WNSFRCHCYS
410
GWAGPTCLEP KP
Length:412
Mass (Da):46,132
Last modified:July 27, 2011 - v2
Checksum:iB4EB888C556BE920
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti228 – 2281R → Q in AAL06145 (PubMed:11929860).Curated
Sequence conflicti228 – 2281R → Q in AAH18457 (PubMed:15489334).Curated
Sequence conflicti296 – 2961F → S in AAL54883 (Ref. 3) Curated
Sequence conflicti296 – 2961F → S in AAL57175 (Ref. 3) Curated
Sequence conflicti354 – 3541A → D in AAL06145 (PubMed:11929860).Curated
Sequence conflicti354 – 3541A → D in AAH18457 (PubMed:15489334).Curated
Sequence conflicti397 – 3971H → R in AAL54882 (Ref. 2) Curated
Sequence conflicti397 – 3971H → R in AAL54883 (Ref. 3) Curated
Sequence conflicti397 – 3971H → R in AAL57175 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY048681 mRNA. Translation: AAL06145.1.
AF074489 mRNA. Translation: AAL54882.1.
AF075576 Genomic DNA. Translation: AAL54883.1.
AF338323 Genomic DNA. Translation: AAL57175.1.
AL672219 Genomic DNA. Translation: CAP19324.1.
BC018457 mRNA. Translation: AAH18457.1.
CCDSiCCDS23499.1.
RefSeqiNP_821139.2. NM_178020.3.
UniGeneiMm.447916.

Genome annotation databases

EnsembliENSMUST00000040059; ENSMUSP00000042667; ENSMUSG00000036091.
ENSMUST00000148440; ENSMUSP00000119499; ENSMUSG00000036091.
GeneIDi109685.
KEGGimmu:109685.
UCSCiuc009rmc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY048681 mRNA. Translation: AAL06145.1.
AF074489 mRNA. Translation: AAL54882.1.
AF075576 Genomic DNA. Translation: AAL54883.1.
AF338323 Genomic DNA. Translation: AAL57175.1.
AL672219 Genomic DNA. Translation: CAP19324.1.
BC018457 mRNA. Translation: AAH18457.1.
CCDSiCCDS23499.1.
RefSeqiNP_821139.2. NM_178020.3.
UniGeneiMm.447916.

3D structure databases

ProteinModelPortaliQ8VEI3.
SMRiQ8VEI3. Positions 26-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042667.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Proteomic databases

PRIDEiQ8VEI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040059; ENSMUSP00000042667; ENSMUSG00000036091.
ENSMUST00000148440; ENSMUSP00000119499; ENSMUSG00000036091.
GeneIDi109685.
KEGGimmu:109685.
UCSCiuc009rmc.2. mouse.

Organism-specific databases

CTDi8372.
MGIiMGI:1330288. Hyal3.

Phylogenomic databases

eggNOGiNOG69101.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ8VEI3.
KOiK01197.
OMAiWEEWCPL.
OrthoDBiEOG74J97S.
PhylomeDBiQ8VEI3.
TreeFamiTF321598.

Enzyme and pathway databases

BRENDAi3.2.1.35. 3474.

Miscellaneous databases

NextBioi362575.
PROiQ8VEI3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VEI3.
CleanExiMM_HYAL3.
ExpressionAtlasiQ8VEI3. baseline and differential.
GenevisibleiQ8VEI3. MM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR027260. Hyaluronidase-3.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PTHR11769:SF19. PTHR11769:SF19. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500776. Hyaluronidase_3. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the murine hyaluronidase gene region reveals complex organization and cotranscription of Hyal1 with downstream genes, Fus2 and Hyal3."
    Shuttleworth T.L., Wilson M.D., Wicklow B.A., Wilkins J.A., Triggs-Raine B.L.
    J. Biol. Chem. 277:23008-23018(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: CD-1.
  2. "Cloning and characterization of human and mouse HYAL3, a third somatic hyaluronidase paralog."
    Csoka A.B.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Genomic sequence of the mouse Hyal1 locus encoding the mouse Hyal1, Fus2, and Hyal3 genes."
    Csoka A.B.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiHYAL3_MOUSE
AccessioniPrimary (citable) accession number: Q8VEI3
Secondary accession number(s): B1AV95, Q8VBX7, Q8VI77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.